ABSTRACT
A lupinosis-associated myopathy occurred in 26 of 48 sheep given a crude toxic extract of Phomopsis leptostromiformis, and in 18 of 34 sheep that grazed a toxic lupin stubble. Treatment with selenium or alpha-tocopherol alone neither prevented nor cured the myopathy, but selenium and alpha-tocopherol together may have been partially effective. Among the group of 48 intoxicated sheep, those with myopathy had a significantly lower mean terminal concentration of alpha-tocopherol in their livers than those with no myopathy. There was no relationship between the severity of liver injury and the occurrence of the myopathy. It was considered that this lupinosis-associated myopathy may have a similar pathogenesis to nutritional myopathy. Data on plasma creatine phosphokinase and erythrocyte glutathione peroxidase activities, plasma alpha-tocopherol concentrations and terminal tissue concentrations of selenium and alpha-tocopherol are presented.
Subject(s)
Fabaceae/microbiology , Mitosporic Fungi , Muscular Diseases/veterinary , Mycotoxins/poisoning , Plants, Medicinal , Sheep Diseases/etiology , Animals , Creatine Kinase/blood , Erythrocytes/enzymology , Glutathione Peroxidase/blood , Liver/chemistry , Liver/enzymology , Liver/pathology , Male , Muscles/chemistry , Muscles/pathology , Muscular Diseases/drug therapy , Muscular Diseases/etiology , Muscular Diseases/prevention & control , Myocardium/chemistry , Plant Poisoning/etiology , Plant Poisoning/veterinary , Random Allocation , Selenium/analysis , Selenium/blood , Selenium/therapeutic use , Sheep , Sheep Diseases/drug therapy , Sheep Diseases/prevention & control , Vitamin E/analysis , Vitamin E/blood , Vitamin E/therapeutic useABSTRACT
SDS-polyacrylamide gradient gel electrophoresis is presented as a technique with potential for the identification of maximum processing temperature in cooked meat products derived from several species. The method is applicable to the examination of these products where evidence to confirm heat inactivation of viruses may be required for quarantine purposes. The methodology for sample preparation, electrophoresis and visualization of separated proteins and an estimate of molecular size of individual proteins is given.
