ABSTRACT
In this study, we investigated the use of a natural process called microbial induced calcium carbonate precipitation (MICP) to 'grow' bio-bricks using the urea present in human urine. We first collected fresh urine and stabilized the urine with calcium hydroxide. This prevented any significant loss of urea which allowed it to then be used for the MICP process. We used Sporosarcina pasteurii bacteria to help drive the MICP process. The bacteria degraded the urea present in the urine to form carbonate ions which then combined with the calcium ions present in the urine solution to produce calcium carbonate. This calcium carbonate was then used as a bio-cement to glue loose sand particles together in the shape of a brick. The maximum compressive strength we obtained for a bio-brick was 2.7â¯MPa which compares well with conventionally made bricks. We successfully showed that human urine can be used to manufacture bio-bricks thus offering an additional use of human urine.
Subject(s)
Bacillus , Sporosarcina , Calcium Carbonate , Chemical Precipitation , Construction Materials , Humans , UreaABSTRACT
Restriction fragment length polymorphism (RFLP) in the large ribosomal RNA region of the mitochondrial DNA (mtDNA) was developed as a genetic marker for investigating mitochondrial transmission in sexual crosses of the human pathogenic basidiomycetous yeast Cryptococcus neoformans. Strain JEC20 of C. neoformans var. neoformans (mat a) was mated with six strains of C. neoformans var. grubii (mat alpha). Successful mating was indicated by the formation of hyphae and basidiospores. These basidiospores were examined for mtDNA RFLP genotypes. All 570 basidiospores examined from the six crosses showed the mtDNA genotype of strain JEC20. The failure to recover the C. neoformans var. grubii mtDNA in any cross indicates that the C. neoformans var. grubii mtDNA is either selectively eliminated in the newly formed dikaryon or selectively excluded in the immediate dikaryotic hyphae of the newly formed dikaryon.
