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1.
Zootaxa ; 5220(1): 1-81, 2022 Dec 15.
Article in English | MEDLINE | ID: mdl-37044546

ABSTRACT

The species- and genus-level taxonomy of Australian species assigned to Opilo Latreille is assessed and revised in the context of a morphological species concept. Australian species are deemed not congeneric with Opilo mollis (Linnaeus), the type species of Opilo Latreille. Species are grouped into genera by degree of difference observed in states of the tegmen, median lobe, antennal scape, basitarsi, hindwing, elytral striae and pronotal disc. Examination of 1,259 specimens resulted in recognition of 6 genera and 44 species, 36 of which are new to science. The following new genera are proposed: Ancyropilus gen. nov.; Culcipternulus gen. nov.; Infectostriatus gen. nov.; Monilonotum gen. nov.; Notopilo gen. nov.; Platynotum gen. nov.. Three infraspecific taxa described as 'varieties', viz, Opilo congruus var. abdominalis Schenkling, 1901, Notoxus congruus var. femoralis Westwood, 1849 and Opilo pascoei var. doddi Schenkling, 1906, are elevated to species status, with Opilo abdominalis (occupied by Opilo abdominalis Fairmaire, 1891) requiring a replacement name. The 44 species recognised in this revision are: Ancyropilus brigalowae sp. nov.; Ancyropilus emmotti sp. nov.; Ancyropilus exossuarius sp. nov.; Ancyropilus hornensis sp. nov.; Ancyropilus labris sp. nov.; Ancyropilus monteithi sp. nov.; Ancyropilus noonbahensis sp. nov.; Ancyropilus packsaddlensis sp. nov.; Ancyropilus simplex sp. nov.; Ancyropilus tricolor sp. nov.; Culcipternulus mareebensis sp. nov.; Infectostriatus absentis sp. nov.; Infectostriatus differens sp. nov.; Monilonotum bunyense sp. nov.; Monilonotum doddi (Schenkling, 1906) stat. rev., comb. nov. (transferred from Opilo); Monilonotum eburneocincta (Gorham, 1878) comb. nov. (transferred from Opilo); Monilonotum pascoei (Gorham, 1876) comb. nov. (transferred from Opilo); Monilonotum rufiventre (Gerstmeier, 1990) comb. nov. (transferred from Olesterus); Monilonotum sundholmi sp. nov.; Notopilo beswickensis sp. nov.; Notopilo brevistriatus sp. nov.; Notopilo calicis sp. nov.; Notopilo cambageicola sp. nov.; Notopilo confusus sp. nov.; Notopilo congruus (Newman) comb. nov. (transferred from Opilo); Notopilo elstoni sp. nov.; Notopilo eremosus sp. nov.; Notopilo gerstmeieri sp. nov.; Notopilo interfabulatus sp. nov.; Notopilo katherinensis sp. nov.; Notopilo lawnhillensis sp. nov.; Notopilo magnus sp. nov.; Notopilo obesus sp. nov.; Notopilo reduncus stat, rev., nom. nov. (replacement name for Opilo abdominalis Schenkling, 1901); Notopilo tanybasilaris sp. nov.; Notopilo tompricensis sp. nov.; Notopilo variipes (Chevrolat) comb. nov. (transferred from Opilo); Notopilo xanthoimprocerus sp. nov.; Notopilo xanthoprolatus sp. nov.; Platynotum bulli sp. nov.; Platynotum culgoense sp. nov.; Platynotum femorale (Westwood) stat. rev., comb. nov. (transferred from Opilo); Platynotum foveosetosa sp. nov.; Platynotum gracile sp. nov.. The available names Opilus congruus Newman, 1842 and Opilus femoralis Westwood, 1849, are respectively corrected to Opilo congruus Newman, 1842 and Opilo femoralis Westwood, 1849 in accordance with Article 11.9.3.2 of the Code (ICZN 1999). Lectotypes are designated for Opilo abdominalis Schenkling, 1901, Opilo congruus Newman, 1842, Opilo femoralis Westwood, 1849, Opilo variipes Chevrolat, Opilo doddi Schenkling, 1906 and Opilo pascoei Gorham, 1876.


Subject(s)
Coleoptera , Animals , Australia , Coleoptera/classification
2.
Cladistics ; 37(3): 276-297, 2021 06.
Article in English | MEDLINE | ID: mdl-34478201

ABSTRACT

Bombyliidae is a very species-rich and widespread family of parasitoid flies with more than 250 genera classified into 17 extant subfamilies. However, little is known about their evolutionary history or how their present-day diversity was shaped. Transcriptomes of 15 species and anchored hybrid enrichment (AHE) sequence captures of 86 species, representing 94 bee fly species and 14 subfamilies, were used to reconstruct the phylogeny of Bombyliidae. We integrated data from transcriptomes across each of the main lineages in our AHE tree to build a data set with more genes (550 loci versus 216 loci) and higher support levels. Our overall results show strong congruence with the current classification of the family, with 11 out of 14 included subfamilies recovered as monophyletic. Heterotropinae and Mythicomyiinae are successive sister groups to the remainder of the family. We examined the evolution of key morphological characters through our phylogenetic hypotheses and show that neither the "sand chamber subfamilies" nor the "Tomophthalmae" are monophyletic in our phylogenomic analyses. Based on our results, we reinstate two tribes at the subfamily level (Phthiriinae stat. rev. and Ecliminae stat. rev.) and we include the genus Sericosoma Macquart (previously incertae sedis) in the subfamily Oniromyiinae, bringing the total number of bee fly subfamilies to 19. Our dating analyses indicate a Jurassic origin of the family (165-194 Ma), with the sand chamber evolving early in bee fly evolution, in the late Jurassic or mid-Cretaceous (100-165 Ma). We hypothesize that the angiosperm radiation and the hothouse climate established during the late Cretaceous accelerated the diversification of bee flies, by providing an expanded range of resources for the parasitoid larvae and nectarivorous adults.


Subject(s)
Bees/classification , Biodiversity , Evolution, Molecular , Larva/physiology , Phylogeny , Transcriptome , Animals , Bees/genetics , Bees/physiology , Larva/genetics , Sequence Analysis, DNA
3.
Zootaxa ; 4456(1): 1-71, 2018 Aug 06.
Article in English | MEDLINE | ID: mdl-30314190

ABSTRACT

The synchronous firefly genus Pteroptyx Olivier is reassessed from morphological, molecular, and habitat perspectives in Malaysia, and includes some reliably associated females described from morphological features and internal female reproductive anatomy. Phylogenetic analyses using combined morphological and molecular data (where available) for 158 taxa supported all the major features of the existing taxonomic categories within the Indopacific Luciolinae. They revealed a distinct Pteroptyx clade as a morphologically variable genus with Poluninius selangoriensis Ballantyne being newly synonymised with Luciola testacea Motschulsky, the type species, which is redescribed from the type series. Pteroptyx gelasina Ballantyne was shown to be distinct and three of the four morphological subdivisions within Pteroptyx malaccae (Gorham) considered useful. A new species Pt. balingiana Jusoh sp. nov. is described from Sarawak. A second specimen of Pt. gombakia Ballantyne is described and figured.        Some females were reliably associated with identified males by molecular data, but investigation of their morphology showed consistent features that were for the most part not useful for species delineation, which still relies on association with the males and colour patterns. All females investigated had bursa plates.Habitat details for most Pteroptyx revealed an association with a riparian environment likely to support mangroves but not necessarily an obligatory association with mangroves or any particular species. Pteroptyx galbina Jusoh was found up to 30 km from the sea, and Pt. bearni Olivier displays in a variety of flowering plants alongside rivers, including mangroves.Keys to species and diagnoses of all species with coloured plates are given.


Subject(s)
Coleoptera , Fireflies , Animals , Ecosystem , Female , Malaysia , Male , Phylogeny
4.
Zootaxa ; 4170(2): 201-249, 2016 Sep 23.
Article in English | MEDLINE | ID: mdl-27701260

ABSTRACT

Sclerotia Ballantyne gen. nov. is established for seven species. Sclerotia aquatilis (Thancharoen, 2007), Scl. brahmina (Bourgeois, 1890), Scl. carinata (Gorham, 1880), Scl. flavida (Hope, 1845), Scl. seriata (Olivier, 1891), and Scl. substriata (Gorham, 1880) are transferred from Luciola Laporte. Sclerotia fui sp. nov. from China is described as new. Luciola cingulata Olivier, 1885 is synonymised with Luciola substriata Gorham. Luciola formosana Pic, 1916 is removed from synonymy with Luciola substriata and newly synonymised with Luciola flavida (Hope). Males are characterised by linear elytral punctation, a set of three sclerites surrounding the aedeagal sheath and a light organ in ventrite 7 with emarginated anterior margin. Associated females lack bursa plates. Larvae associated for three species are back swimmers, having heavily sclerotised exoskeleton and a metapneustic respiratory system. Males and associated females are keyed. Analysis of molecular evidence for Chinese populations of three species indicates monophyly of Sclerotia Ballantyne gen. nov. and distinct separation from other genera.Triangulara Pimpasalee gen. nov. is described from Tri. frontoflava Pimpasalee gen. et sp. nov. from Thailand. It is superficially similar to Sclerotia gen. nov. but larger, and specimens in collections appear to have been confused with the similarly coloured Scl. substriata (Gorham) comb. nov. Males differ in having an elongate triangular light organ in ventrite 7 and are without sclerites around the aedeagal sheath. Larvae are not reliably associated but may be aquatic.


Subject(s)
Fireflies/anatomy & histology , Fireflies/classification , Animals , China , Female , Fireflies/growth & development , Larva/anatomy & histology , Male , Species Specificity , Swimming , Thailand
5.
Zootaxa ; 3959: 1-84, 2015 May 20.
Article in English | MEDLINE | ID: mdl-26249372

ABSTRACT

The Luciolinae firefly genus Pyrophanes is redescribed and a key is provided for 7 species, viz. appendiculata Olivier, beccarii Olivier, quadrimaculata Olivier, semilimbata (Olivier) and similis Olivier, with Py. elongata Ballantyne sp. nov. and Py. similisimma Ballantyne sp. nov. described as new. Macropterous females associated for several species have 2 pairs of broad bursa plates. Following an enlarged phylogenetic analysis of morphological characters, an expanded generic description of Pteroptyx Olivier now accommodates four new species with metafemoral combs. Three of these have an entire light organ in ventrite 7 but are without the generic hallmark of deflexed elytral apices in the male. The first species of Pteroptyx to possess these features are Pt. sayangia Ballantyne sp. nov., Pt. galbina Jusoh sp. nov. and Pt. surabayia Ballantyne sp. nov. Based on a single unusual specimen Pt. gombakia Ballantyne sp. nov. is here described with deflexed elytral apices and bipartite light organ in ventrite 7, but with exceptional incurving lobes along the posterior margin of ventrite 7, which is a characteristic of Pyrophanes. The description of Luciola testacea Motschulsky is discussed and many morphological characters of its type specimen are correctly outlined. Its place as the type for Pteroptyx is addressed. Inflata gen. nov. Boontop is erected for specimens of Luciola indica Motschulsky as Inflata indica (Motsch.) comb. nov., and is described from males and reliably associated females. Discussion overviews the present situation regarding Luciolinae taxonomy of southeast Asian and the Australopacific regions.


Subject(s)
Fireflies/classification , Animal Distribution , Animal Structures/anatomy & histology , Animal Structures/growth & development , Animals , Asia , Body Size , Female , Fireflies/anatomy & histology , Fireflies/genetics , Fireflies/growth & development , Male , Organ Size , Phylogeny
6.
Zootaxa ; 3881(4): 301-27, 2014 Nov 06.
Article in English | MEDLINE | ID: mdl-25543638

ABSTRACT

The endemic Australian flower chafer genus Metallesthes Kraatz, 1880 (Coleoptera: Scarabaeidae: Cetoniinae) is revised. Metallesthes anneliesae Moeseneder & Hutchinson new species is described from southern Queensland and New South Wales. Metallesthes unicolor (Macleay, 1863) revised status is raised from synonymy with Metallesthes metallescens (White, 1859). Metallesthes metallescens and Metallesthes unicolor are redescribed and their holotypes are figured. A specimen bearing a Nonfried type label is designated as the lectotype of Metallesthes subpilosa Nonfried, 1891. Metallesthes subpilosa new synonymy is synonymised with Pseudoclithria ruficornis (Westwood, 1874). A key to the species of the genus is provided. Distribution maps are shown and known host plants are listed.


Subject(s)
Animal Distribution , Coleoptera/anatomy & histology , Coleoptera/classification , Animals , Coleoptera/physiology , Female , Male , New South Wales , Queensland , Species Specificity
7.
Zootaxa ; (3802): 553-82, 2014 May 28.
Article in English | MEDLINE | ID: mdl-24871028

ABSTRACT

We describe, diagnose and illustrate eight new species in the genus Anabarhynchus Macquart, 1848 as follows: Anabarhynchus cretatus sp. n., Anabarhynchus darembal sp. n., Anabarhynchus iancommoni sp. n., Anabarhynchus longiseta sp. n. Anabarhynchus lyncurium sp. n., Anabarhynchus moretonensis sp. n., Anabarhynchus neboensis sp. n. and Anabarhynchus wintertoni sp. n. These represent all new species in collections from south east Queensland. These new species bring the total number of described Australian species in the genus to 112.


Subject(s)
Diptera/anatomy & histology , Diptera/classification , Animals , Female , Male , Queensland
8.
Zootaxa ; 3721: 1-48, 2013.
Article in English | MEDLINE | ID: mdl-26120657

ABSTRACT

Abscondita, a new genus of fireflies from South-east Asia, is described from males and females of Abs. anceyi (Olivier 1883), Abs. cerata (Olivier 1911), Abs. chinensis (L. 1767), Abs. perplexa (Walker 1858), Abs. promelaena (Walker 1858) and Abs. terminalis (Olivier 1883), all transferred from Luciola Laporte. Both L. dubia Olivier 1903 and L. dejeani Gemminger 1870 are synonymised with Luciola perplexa (Walker), and L. aegrota Olivier 1891 and L. melaspis Bourgeois 1909 with L. promelaena Walker. Females are characterised by their bursa plates. Larvae are associated and described for Abs. anceyi (Olivier), Abs. chinensis (L.) and Abs. terminalis (Olivier). Taxonomic issues regarding the identification of species with very similar colouration of pale dorsum and black tipped elytra are addressed and in some cases resolved. A neotype for Luciola chinensis (L.) is erected and Luciola praeusta (Kiesenwetter 1874) is synonymised with L. chinensis (L.). Descriptions of life histories, biology and flashing patterns of populations of Abs. chinensis and Abs. terminalis from central China are included. A bs. terminalis is the first Asian firefly known to possess multiple flash trains where males are documented to display with repeating flash trains.


Subject(s)
Fireflies/classification , Animal Structures/anatomy & histology , Animal Structures/growth & development , Animals , Asia , Body Size , Female , Fireflies/anatomy & histology , Fireflies/genetics , Fireflies/growth & development , Larva/anatomy & histology , Larva/classification , Larva/genetics , Larva/growth & development , Life Cycle Stages , Male , Phylogeny , Sexual Behavior, Animal
9.
Zootaxa ; 3681: 371-94, 2013.
Article in English | MEDLINE | ID: mdl-25232616

ABSTRACT

The Australian endemic genus Enhypnon Carter (Coleoptera, Zopheridae) is revised, including the description of six new species: E. cordicollis sp. n., E. kosciuszko sp. n., E. simplex sp. n., E. squamosum sp. n., E. latitarsis sp. n., and E. tuberculatum sp. n., and the re-description of four species: E. costatum (Carter, 1919) comb. n. (from Mnionophilus), E. horridum (Carter, 1919), E. laticeps Carter, 1919 and E. punctatum Carter, 1927. A lectotype and paralectotype are herein designated for E. laticeps. A key to species is provided. All species are illustrated with line-drawings and high-resolution photographs. Detailed locality records are given for each species and distribution maps are provided. Distribution and habitat preference data are discussed.


Subject(s)
Coleoptera/classification , Animal Distribution , Animal Structures/anatomy & histology , Animals , Australia , Coleoptera/anatomy & histology , Ecosystem , Female , Male
10.
Zootaxa ; 3653: 1-162, 2013.
Article in English | MEDLINE | ID: mdl-25340191

ABSTRACT

This revision completes a taxonomic survey of fireflies (Coleoptera: Lampyridae) in the area encompassed by Australia, the Republic of Palau, Federated States of Micronesia, Papua New Guinea, Indonesia (West Irian/Papua), Solomon Islands, New Caledonia, Vanuatu and Fiji. It finalises the taxonomic issues arising from the 1969­70 voyage of the scientific vessel Alpha Helix to New Guinea. The firefly fauna of this area is exclusively Luciolinae. The scope of the revision was extended to include all known Luciolinae genera and certain species from SE Asia, and a phylogenetic analysis of 436 morphological characters of males, females, and associated larvae includes 142 Luciolinae species (Ballantyne & Lambkin 2009, and Fu et al. 2012a). The phylogenetic analyses infer four major groups within the Luciolinae. The monotypic Missimia Ballantyne is sister to all remaining Luciolinae and forms a grade to Aquatica Fu etBallantyne. The large clade of Curtos Motschulsky, Photuroluciola Pic, Colophotia Motschulsky, Poluninius gen. nov., Pyrophanes Olivier, Pteroptyx s. str. Olivier, Medeopteryx gen. nov., Trisinuata gen. nov., and Australoluciola gen. nov.forms a grade to the clade of Luciola s. str. Laporte (including Bourgeoisia Olivier). The monotypic Emeia Fu et al.forms a grade with a clade of Luciola and Pygoluciola Wittmer, sister to a large clade of Convexa Ballantyne, Pacifica gen. nov., Magnalata Ballantyne, Lloydiella Ballantyne, Asymmetricata Ballantyne, Pygatyphella s. str. Ballantyne, Atyphella Olliff, Aquilonia Ballantyne, and Gilvainsula Ballantyne. Luciola is paraphyletic, found in up to six clades across the tree. Together with Luciola, Magnalata, Aquilonia, and Gilvainsula render Atyphella paraphyletic. The new genera described here are all monophyletic and supported in the phylogenetic analyses that also provide evidence for the inclusion of taxa within them. Twenty-three genera including five new ones, and ten new species, are recognised and keys are presented for the males and females. Certain females are characterised by the nature of their bursa plates. Australoluciola gen. nov. is proposed for ten species from Australia and New Guinea, seven transferred from Luciola and three new, with species keyed from males, all of which have an entire light organ in ventrite 7. Aus. anthracina (Olivier), Aus. aspera (Olivier), Aus. australis (F.), Aus. flavicollis (MacLeay), Aus. foveicollis (Olivier), Aus. nigra (Olivier) and Aus. orapallida (Ballantyne) are transferred from Luciola with males assigned to Aus. aspera(Olivier), and a lectotype designated for Luciola foveicollis Olivier; Aus. baduria sp. nov., Aus. fuscamagna sp. nov.,Aus. fuscaparva sp. nov., Aus. japenensis sp. nov. and Aus. pharusaurea sp. nov. are described. Females of Aus. australis and Aus. flavicollis have two pairs of wide bursa plates.  The bent-winged fireflies of New Guinea and Australia are removed from Pteroptyx Olivier and assigned to Medeopteryx gen. nov. and Trisinuata gen. nov. Medeopteryx gen. nov. is erected for 17 species including two new; all have ventrite 7 with an entire light organ, trisinuate posterior margin and short posterolateral projections; the following 14 species in which males have deflexed elytral apices are transferred from Pteroptyx Olivier: M. amilae (Satô), M. antennata (Olivier), M. corusca (Ballantyne), M. cribellata (Olivier), M. effulgens (Ballantyne), M. elucens (Ballantyne), M. flagrans (Ballantyne), M. fulminea (Ballantyne), M. hanedai (Ballantyne), M. platygaster (Lea), M. similisantennata(Ballantyne), M. sublustris (Ballantyne), M. tarsalis (Olivier), and M. torricelliensis (Ballantyne). M. clipeata sp. nov. is described. Two species without deflexed elytral apices include M. pupilla (Olivier) which is transferred from Luciola, and M. similispupillae sp. nov. A Lectotype is designated for Luciola pupilla (Olivier). Females of M. corusca(Ballantyne), M. cribellata (Olivier), M. effulgens (Ballantyne), and M. similispupillae sp. nov. have two pairs of wide bursa plates. The second genus including species in which the males have deflexed elytral apices is Trisinuata gen. nov., where all males have light organ in ventrite 7 bipartite and posterolateral projections expanded; it is proposed for eight New Guinean species: T. microthorax (Olivier), T. minor (Ballantyne), T. papuae (McDermott) and T. similispapuae(Ballantyne) are transferred from Pteroptyx Olivier, T. papuana (Olivier) previously known only from a female, has males associated and is transferred from Luciola, and T. caudabifurca sp. nov., T. dimidiata sp. nov. and T. apicula sp. nov. are described. Females of T. similispapuae (Ballantyne) have two pairs of wide bursa plates. Luciola s. str. is defined by scoring the type species L. italica (L), Bourgeoisia Olivier and Lampyroidea (based on its type species syriaca Costa) both of which are submerged into Luciola; Luciola s. str is addressed here from four Pacific Island species: L. hypocrita Olivier, L. antipodum Bourgeois both transferred from Bourgeoisia; L. aquilaclarasp. nov. and L. oculofissa sp. nov. are described. L. oculofissa sp. nov. is the only Luciolinae male known to lack light organs. Females of L. italica and L. hypocrita lack bursa plates.Pacifica gen. nov. is proposed for five species from the Solomon Islands transferred from Pygatyphella(Ballantyne), and which the phylogenetic analysis shows to be distinctive viz. P. limbatifusca (Ballantyne), P. limbatipennis (Pic), P. plagiata (Blanchard), P. russellia (Ballantyne), and P. salomonis (Olivier). A monotypic genus Poluninius gen. nov. is proposed for Pol. selangoriensis sp. nov. from Selangor, Malaysia. The genera Colophotia, Pteroptyx, Pyrophanes, and Pygoluciola are treated in an abbreviated fashion with generic diagnoses, lists of, and keys to, species. Pteroptyx bearni Olivier and P. tener Olivier are characterised from type specimens and female bursae and P. similis Ballantyne is synonymised with P. bearni. Luciola semilimbata Olivier is transferred to Pyrophanes, and Luciola cowleyi Blackburn to Pygoluciola. The following species are treated as species incertae sedis: L. melancholica Olivier, L. ruficollis Guérin-Ménéville. The New Guinean records of Luciola tenuicornis Olivier, L. timida Olivier and Photinus cinctellus Motschulsky are suspect. Fifteen of the species treated here are recognised by flashing patterns. The functions of the terminal abdominal modifications, origins of the Australopacific firefly fauna, and use of female and larval characters in interpretations of relationships are considered.


Subject(s)
Fireflies/classification , Animals , Australia , Female , Fireflies/anatomy & histology , Fireflies/genetics , Indonesia , Larva , Male , Micronesia , Papua New Guinea , Phylogeny
11.
Zookeys ; (240): 1-119, 2012.
Article in English | MEDLINE | ID: mdl-23226714

ABSTRACT

The previously monotypic genus Manestella Metz, 2003 is revised with a single species, Manestella tristriata (Mann, 1933), redescribed and an additional 14 new species described: Manestella caesiasp. n., Manestella campestrissp. n., Manestella canitiessp. n., Manestella cooloolasp. n., Manestella fumosasp. n., Manestella incompletasp. n., Manestella nubissp. n., Manestella obscurasp. n., Manestella ocellarissp. n., Manestella personasp. n., Manestella poecilothoraxsp. n., Manestella umbrapennissp. n., Manestella vastasp. n. and Manestella vesperasp. n. The putative sister genus to Manestella, Medomegagen. n., is described containing six new species: Medomega averyisp. n., Medomega bailmeupsp. n., Medomega chlamydossp. n., Medomega danielsisp. n., Medomega gigasathesp. n., and Medomega nebriassp. n. Complete taxonomic descriptions were generated from a character matrix developed in Lucid Builder from which natural language descriptions (NLD) were parsed. Images of all species of Manestella and Medomegagen. n. are included, along with dichotomous keys to species.

12.
Gene ; 511(2): 131-42, 2012 Dec 15.
Article in English | MEDLINE | ID: mdl-23043935

ABSTRACT

Members of the Calliphoridae (blowflies) are significant for medical and veterinary management, due to the ability of some species to consume living flesh as larvae, and for forensic investigations due to the ability of others to develop in corpses. Due to the difficulty of accurately identifying larval blowflies to species there is a need for DNA-based diagnostics for this family, however the widely used DNA-barcoding marker, cox1, has been shown to fail for several groups within this family. Additionally, many phylogenetic relationships within the Calliphoridae are still unresolved, particularly deeper level relationships. Sequencing whole mt genomes has been demonstrated both as an effective method for identifying the most informative diagnostic markers and for resolving phylogenetic relationships. Twenty-seven complete, or nearly so, mt genomes were sequenced representing 13 species, seven genera and four calliphorid subfamilies and a member of the related family Tachinidae. PCR and sequencing primers developed for sequencing one calliphorid species could be reused to sequence related species within the same superfamily with success rates ranging from 61% to 100%, demonstrating the speed and efficiency with which an mt genome dataset can be assembled. Comparison of molecular divergences for each of the 13 protein-coding genes and 2 ribosomal RNA genes, at a range of taxonomic scales identified novel targets for developing as diagnostic markers which were 117-200% more variable than the markers which have been used previously in calliphorids. Phylogenetic analysis of whole mt genome sequences resulted in much stronger support for family and subfamily-level relationships. The Calliphoridae are polyphyletic, with the Polleninae more closely related to the Tachinidae, and the Sarcophagidae are the sister group of the remaining calliphorids. Within the Calliphoridae, there was strong support for the monophyly of the Chrysomyinae and Luciliinae and for the sister-grouping of Luciliinae with Calliphorinae. Relationships within Chrysomya were not well resolved. Whole mt genome data, supported the previously demonstrated paraphyly of Lucilia cuprina with respect to L. sericata and allowed us to conclude that it is due to hybrid introgression prior to the last common ancestor of modern sericata populations, rather than due to recent hybridisation, nuclear pseudogenes or incomplete lineage sorting.


Subject(s)
DNA Barcoding, Taxonomic , Diptera/genetics , Animals , Polymerase Chain Reaction
13.
Zookeys ; (150): 231-80, 2011.
Article in English | MEDLINE | ID: mdl-22207816

ABSTRACT

Bush Blitz is a three-year multimillion dollar program to document the plants and animals in hundreds of properties across Australia's National Reserve System. The core focus is on nature discovery - identifying and describing new species of plants and animals. The Bush Blitz program has enabled the collection and description of beeflies (Diptera, Bombyliidae) from surveys in Western Australia and Queensland. Three new species of Australian beeflies belonging to the Exoprosopini are described; Palirika mackenziei Lambkin sp. n., Palirika culgoafloodplainensis Lambkin sp. n., and Larrpana bushblitz Lambkin sp. n. Phylogenetic analysis of 40 Australian exoprosopine species belonging to the Balaana generic-group Lambkin & Yeates 2003 supports the placement of the three new species into existing genera, and the erection and description of the new genus Ngalki Lambkin gen. n. for Ngalki trigonium (Lambkin & Yeates 2003) comb. n. Revised keys are provided for the genera of the Australian Balaana genus-group and the species of Palirika Lambkin & Yeates, 2003 and Larrpana Lambkin & Yeates 2003. With the description of the three new species and the transferral of Munjua trigona Lambkin & Yeates 2003 into the new genus Ngalki Lambkin gen. n., three genera are rediagnosed; Munjua Lambkin & Yeates 2003, Palirika and Larrpana.

14.
Proc Natl Acad Sci U S A ; 108(14): 5690-5, 2011 Apr 05.
Article in English | MEDLINE | ID: mdl-21402926

ABSTRACT

Flies are one of four superradiations of insects (along with beetles, wasps, and moths) that account for the majority of animal life on Earth. Diptera includes species known for their ubiquity (Musca domestica house fly), their role as pests (Anopheles gambiae malaria mosquito), and their value as model organisms across the biological sciences (Drosophila melanogaster). A resolved phylogeny for flies provides a framework for genomic, developmental, and evolutionary studies by facilitating comparisons across model organisms, yet recent research has suggested that fly relationships have been obscured by multiple episodes of rapid diversification. We provide a phylogenomic estimate of fly relationships based on molecules and morphology from 149 of 157 families, including 30 kb from 14 nuclear loci and complete mitochondrial genomes combined with 371 morphological characters. Multiple analyses show support for traditional groups (Brachycera, Cyclorrhapha, and Schizophora) and corroborate contentious findings, such as the anomalous Deuterophlebiidae as the sister group to all remaining Diptera. Our findings reveal that the closest relatives of the Drosophilidae are highly modified parasites (including the wingless Braulidae) of bees and other insects. Furthermore, we use micro-RNAs to resolve a node with implications for the evolution of embryonic development in Diptera. We demonstrate that flies experienced three episodes of rapid radiation--lower Diptera (220 Ma), lower Brachycera (180 Ma), and Schizophora (65 Ma)--and a number of life history transitions to hematophagy, phytophagy, and parasitism in the history of fly evolution over 260 million y.


Subject(s)
Adaptation, Biological/genetics , Biological Evolution , Diptera/anatomy & histology , Diptera/genetics , Phylogeny , Animals , Base Sequence , Bayes Theorem , Gene Library , Likelihood Functions , MicroRNAs/genetics , Models, Genetic , Molecular Sequence Data , Sequence Analysis, DNA , Species Specificity
15.
Mol Phylogenet Evol ; 29(1): 120-5, 2003 Oct.
Article in English | MEDLINE | ID: mdl-12967613

ABSTRACT

Fragments of two mitochondrial genes, cytochrome b (CytB) and Cytochrome c oxidase subunit I (COI) have been used as phylogenetic markers in Sergentia (Chironomidae, Diptera). The concatenated (1241 bp) sequences from both genes were used to infer the phylogenetic relationships among seven Sergentia species. Five of the species belong to the endemic fauna of Lake Baikal. Alignments of the nucleotide sequences were used for the construction of trees using Neighbor-Joining and maximum parsimony methods. Both methods yielded similar results. Monophyly of both Sergentia and the Baikalian endemic species was well supported. The date of origin of the endemic group of Sergentia was estimated as 25.7 MYA which closely coincides with the start of geological changes in the Baikal area. A cytological tree, based on 12 chromosomal characteristics, for the same set of Sergentia species showed a great similarity to the molecular phylogeny.


Subject(s)
Chironomidae/genetics , Animals , Chironomidae/classification , Cytochromes b/genetics , DNA, Mitochondrial/genetics , Electron Transport Complex IV/genetics , Evolution, Molecular , Genes, Insect , Phylogeny , Russia
16.
Cladistics ; 18(4): 436-444, 2002 Aug.
Article in English | MEDLINE | ID: mdl-34911216

ABSTRACT

Partitioned Bremer support (PBS) is a valuable means of assessing congruence in combined data sets, but some aspects require clarification. When more than one equally parsimonious tree is found during the constrained search for trees lacking the node of interest, averaging PBS for each data set across these trees can conceal conflict, and PBS should ideally be examined for each constrained tree. Similarly, when multiple most parsimonious trees (MPTs) are generated during analysis of the combined data, PBS is usually calculated on the consensus tree. However, extra information can be obtained if PBS is calculated on each of the MPTs or even suboptimal trees.

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