Studies on bovine Breda virus.

Diarrheic feces from 21 calves were examined by electron microscopy and 16 contained particles morphologically similar to those of Breda virus. The particles were spherical or elongated, 60-270 nm in greatest dimension and had surface spikes 9-13 nm long. Convalescent serum from a human patient with Breda virus-associated diarrhea reacted with one of the bovine viruses by immune electron microscopy, suggesting a serological resemblance between human and bovine Breda-like viruses. Immune electron-microscopy and immunofluorescence demonstrated that isolates of bovine Breda virus from the U.S.A. were related to the French virus. One of the viruses had a density in sucrose solution of 1.16, similar to the value for Berne virus.

[Viral gastroenteritis]. / Les gastroenterites virales.

Many virus are able to induce gastroenteritis, especially in young children with immunological or nutritional defects. The most frequently observed, using ELISA, are Rotavirus. The others : Norwalk agent, Coronavirus, Breda virus, are identified using electron microscope. Using symptomatic therapy, prognosis is related to precocious hydratation.

An enveloped virus in stools of children and adults with gastroenteritis that resembles the Breda virus of calves.

Pleomorphic virus-like particles about 100 nm in diameter with a fringe of closely applied peplomers (7-9 nm in length) were observed by electron microscopy in the stools of 20 children and adults with gastroenteritis. In most of the samples no other viral or bacterial pathogens were detected. In form and under immune electron microscopy these virus-like particles resembled the Breda virus isolated from diarrhoeic calves. These objects may be a viral pathogen of humans.