ABSTRACT
OBJECTIVE: To investigate the retrograde amnesia changes within different injury levels of cerebral concussion in rats. METHODS: A metallic pendulum striker device of brain injury was deployed to duplicate CC models of different injury levels within Sprague-Dawley (S-D) rats. The investigated animals were divided into two groups according to classification standard, that is, Pure Cerebral Concussion (PCC) group and Complicated Cerebral Concussion (CCC) group. One control group was used, and each group included 8 animals. The retrograde amnesia of each group was assessed by Morris Water Maze (MWM) Test from 3 days preinjury to 7 days postconcussion. RESULTS: Compared with the control group, the retrograde amnesia was detected within 3 days in PCC group, and 5 days in CCC group after injury. At the same time, the two groups both manifested space recognition deficit. CONCLUSION: The retrograde amnesia existed in both pure cerebral concussion group and complicated cerebral concussion. Furthermore, the lasting time of retrograde amnesia in animals correlates to the injury level of brain concussion.
Subject(s)
Amnesia, Retrograde/psychology , Brain Concussion/psychology , Maze Learning , Memory , Amnesia, Retrograde/etiology , Animals , Brain Concussion/complications , Brain Concussion/pathology , Disease Models, Animal , Female , Injury Severity Score , Male , Rats , Rats, Sprague-Dawley , Time FactorsABSTRACT
OBJECTIVE: To investigate the effect of continuous renal replacement treatment (CRRT) stresses reaction in patients with severe trauma and its clinical significance. METHODS: Twenty-nine patients with severe trauma were randomly divided into two groups: treatment group (15 cases, treated with continuous veno-venous hemofiltration (CVVH) within 12 hours after trauma) and control group (14 cases, not treated with CVVH). The levels of cortical, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in plasma were determined at 12 hours, 24 hours, 48 hours, 72 hours after trauma, and the temperature, heart beat, respiratory rate, blood pressure were observed. RESULTS: The levels of cortical, IL-6 in both groups increased to some degree in 12 hours. In control group, the level of cortical increased gradually, peaking at 48 hours, and it was still higher than normal at 72 hours. The level of cortical decreased in the treatment group gradually after 24 hours, approaching normal level at 72 hours. When compared with the control group, the levels of cortical in plasma were lower obviously in the treatment group at 24 hours, 48 hours, 72 hours (all P<0.001). The level of IL-6 decreased obviously after 12 hours and was lower evidently at 24 hours,48 hours, 72 hours (all P<0.05). The clinic symptoms were improved obviously. The level of TNF-alpha was increased at each time point in both groups, but there was no significant difference between two groups. CONCLUSION: CRRT can eliminate effectively the stress hormone, and reduce stress reaction obviously. The change in IL-6 is more marked than that of cortical.