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1.
J Environ Manage ; 354: 120415, 2024 Mar.
Article in English | MEDLINE | ID: mdl-38417359

ABSTRACT

Aboveground biomass (AGB) in grasslands directly reflects the net primary productivity, making it a sensitive indicator of grassland resource quality and ecological degradation. Accurately estimating AGB over large regions to reveal long-term AGB evolution trends remains a formidable challenge. In this study, we divided Inner Mongolia Autonomous Region (IMAR) grasslands into three study regions based on their spatial distribution of grassland types. We combined remote sensing data with ground-based sample data collected over the past 19 years from 6114 field plots using the Google Earth Engine platform. We constructed random forest (RF) and traditional regression AGB inversion models for each region and selected the best-performing model through accuracy assessment to estimate IMAR grassland AGB for the period 2000-2022. We also examined the trends in AGB changes and identified the driving forces affecting IMAR grasslands through the application of Theil-Sen estimation, Mann-Kendall trend analysis, and the Geodetector model. The main findings are as follows: (1) Compared with the univariate parametric traditional regression model, the AGB monitoring accuracy of the multivariate non-parametric RF model in the three study regions increased by 5.94%, 5.08% and 19.14%, respectively. (2) The average AGB per unit area of IMAR grasslands from 2000 to 2022 was 731.41 kg/hm2, with alpine meadow having the highest average AGB (1271.70 kg/hm2) and temperate grassland desertification having the lowest (469.06 kg/hm2). IMAR grasslands exhibited an overall increasing trend in AGB over the past 23 years (6.01 kg/hm2•yr), with the increasing trend covering 83.52% of the grassland area and the decreasing trend covering 16.48%. (3) Spatially, IMAR grassland AGB showed a gradual decline from northeast to southwest and exhibited an increasing trend with increasing longitude (45.423 kg/hm2 per degree) and latitude (71.9 kg/hm2 per degree). (4) Meteorological factors were the most significant factors affecting IMAR grassland AGB, with precipitation (five-year average q value of 0.61) being the most prominent. In the western part of IMAR, where precipitation is consistently limited throughout the year, the primary drivers of influence were human activities, with particular emphasis on the number of livestock (with a five-year average q value of 0.44). It is evident that reducing human activity disturbance and pressure in fragile grassland areas or implementing near-natural restoration measures will be beneficial for the sustainable development of grassland ecosystems. The results of this research hold substantial reference importance for the protection and restoration of grasslands, the supervision and administration of grassland resources, as well as the development of policies related to grassland management.


Subject(s)
Ecosystem , Grassland , Animals , Humans , Biomass , China , Livestock
2.
Opt Express ; 31(24): 40249-40260, 2023 Nov 20.
Article in English | MEDLINE | ID: mdl-38041330

ABSTRACT

An adaptive Fourier neural operator (AFNO)-transformer model was developed to retrieve land surface temperature (LST) data from infrared atmospheric sounding interferometer (IASI) observations. A weight selection scheme based on linearization of the radiative transfer equation was proposed to solve the hyperspectral data channel redundancy problem. The IASI brightness temperatures and Advanced Very High Resolution Radiometer onboard MetOp (AVHRR/MetOp) LST product were selected to construct the training and test datasets. The AFNO-transformer performed effective token mixing through self-attention and effectively solved the global convolution problem in the Fourier domain, which can better learn complex nonlinear equations and achieve time-series forecasting. The root mean square error indicated that the LST in Eastern Spain and North Africa could be retrieved with an error of less than 2.5 K compared with the AVHRR/MetOp LST product. Moreover, the validation results from other time period data showed that the retrieval accuracy of this model can be less than 3 K. The proposed model provides a novel approach for hyperspectral LST retrieval.

3.
Front Microbiol ; 14: 1232279, 2023.
Article in English | MEDLINE | ID: mdl-37577430

ABSTRACT

Potato virus Y (PVY) infection causes necrosis and curling of leaves, which seriously affect the yield and quality of Solanaceous crops. The roles of nutrient elements in the regulation of plant resistance to virus infection has been widely reported, while the mechanisms are poorly studied. Previous studies in our laboratory have demonstrated that foliar spraying of MgSO4 could induce Nicotiana tabacum resistance to PVY by increasing the activity of defense-related enzymes. Consistent with the results, we found that exogenous magnesium (Mg) had a certain effect on N. tabacum anti-PVY infection. Meanwhile, Illumina RNA sequencing revealed that Mg induced resistance to PVY infection was mainly by regulating carbohydrate metabolism and transportation, nitrogen metabolism, Ca2+ signal transduction and oxidative phosphorylation. Moreover, we used virus-induced gene silencing assays to verify the function of homologs of five N. tabacum genes involved in above pathways in N. benthamiana. The results showed that NbTPS and NbGBE were conducive to PVY infection, while NbPPases and NbNR were related to resistance to PVY infection. These results suggested a novel strategy for resistance to PVY infection and provided a theoretical basis for virus-resistance breeding.

4.
Clin. transl. oncol. (Print) ; 25(6): 1545-1553, jun. 2023.
Article in English | IBECS | ID: ibc-221189

ABSTRACT

Acute myeloid leukemia (AML) is an aggressive hematologic cancer in adults. Some patients exhibit restricted T cell infiltration and do not respond to routine treatments. This may be prevented by enhancing adaptive immunity by stimulating innate immune cells inside the tumor microenvironment (TME). To activate the adaptive immunological reaction against tumors, type I interferons (IFNs) can promote the presentation of tumor-specific cytotoxic T lymphocyte (CTL) cell recruitment. During the activation of innate immunity, cyclic di-nucleotides (CDNs) bind to and stimulate the stimulator of interferon genes (STING), a protein localized inside the endoplasmic reticulum (ER) membrane, resulting in the expression of type I IFNs. The efficacy of STING agonists as effective stimulators of the anti-tumor response in AML is being investigated in numerous clinical studies. Therefore, the purpose of this investigation was to thoroughly review existing knowledge in this field and provide perspective into the clinical potential of STING agonists in AML (AU)


Subject(s)
Humans , Immunity, Innate , Interferons/administration & dosage , Leukemia, Myeloid, Acute/genetics , Leukemia, Myeloid, Acute/drug therapy , Antineoplastic Agents, Immunological/therapeutic use , Nucleotides, Cyclic , Tumor Microenvironment , Adaptive Immunity
5.
Front Plant Sci ; 14: 1163679, 2023.
Article in English | MEDLINE | ID: mdl-37063211

ABSTRACT

Potato virus Y (PVY) mainly infects Solanaceous crops, resulting in considerable losses in the yield and quality. Iron (Fe) is involved in various biological processes in plants, but its roles in resistance to PVY infection has not been reported. In this study, foliar application of Fe could effectively inhibit early infection of PVY, and a full-length transcriptome and Illumina RNA sequencing was performed to investigate its modes of action in PVY-infected Nicotiana tabacum. The results showed that 18,074 alternative splicing variants, 3,654 fusion transcripts, 3,086 long non-coding RNAs and 14,403 differentially expressed genes (DEGs) were identified. Specifically, Fe application down-regulated the expression levels of the DEGs related to phospholipid hydrolysis, phospholipid signal, cell wall biosynthesis, transcription factors (TFs) and photosystem I composition, while those involved with photosynthetic electron transport chain (PETC) were up-regulated at 1 day post inoculation (dpi). At 3 dpi, these DEGs related to photosystem II composition, PETC, molecular chaperones, protein degradation and some TFs were up-regulated, while those associated with light-harvesting, phospholipid hydrolysis, cell wall biosynthesis were down-regulated. At 9 dpi, Fe application had little effects on resistance to PVY infection and transcript profiles. Functional analysis of these potentially critical DEGs was thereafter performed using virus-induced gene silencing approaches and the results showed that NbCat-6A positively regulates PVY infection, while the reduced expressions of NbWRKY26, NbnsLTP, NbFAD3 and NbHSP90 significantly promote PVY infection in N. benthamiana. Our results elucidated the regulatory network of Fe-mediated resistance to PVY infection in plants, and the functional candidate genes also provide important theoretical bases to further improve host resistance against PVY infection.

6.
Clin Transl Oncol ; 25(6): 1545-1553, 2023 Jun.
Article in English | MEDLINE | ID: mdl-36587109

ABSTRACT

Acute myeloid leukemia (AML) is an aggressive hematologic cancer in adults. Some patients exhibit restricted T cell infiltration and do not respond to routine treatments. This may be prevented by enhancing adaptive immunity by stimulating innate immune cells inside the tumor microenvironment (TME). To activate the adaptive immunological reaction against tumors, type I interferons (IFNs) can promote the presentation of tumor-specific cytotoxic T lymphocyte (CTL) cell recruitment. During the activation of innate immunity, cyclic di-nucleotides (CDNs) bind to and stimulate the stimulator of interferon genes (STING), a protein localized inside the endoplasmic reticulum (ER) membrane, resulting in the expression of type I IFNs. The efficacy of STING agonists as effective stimulators of the anti-tumor response in AML is being investigated in numerous clinical studies. Therefore, the purpose of this investigation was to thoroughly review existing knowledge in this field and provide perspective into the clinical potential of STING agonists in AML.


Subject(s)
Immunity, Innate , Leukemia, Myeloid, Acute , Humans , Leukemia, Myeloid, Acute/drug therapy , Leukemia, Myeloid, Acute/genetics , Nucleotides, Cyclic , Adaptive Immunity , Interferons , Immunotherapy/methods , Tumor Microenvironment
7.
CNS Neurosci Ther ; 29(1): 78-90, 2023 01.
Article in English | MEDLINE | ID: mdl-36221783

ABSTRACT

INTRODUCTION: SHANK3 is an important excitatory postsynaptic scaffold protein, and its mutations lead to genetic cause of neurodevelopmental diseases including autism spectrum disorders (ASD), Philan McDermid syndrome (PMS), and intellectual disability (ID). Early prevention and treatment are important for Shank3 gene mutation disease. Swimming has been proven to have a positive effect on neurodegenerative diseases. METHODS: Shank3 gene exon 11-21 knockout rats were intervened by a 40 min/day, 5 day/week for 8-week protocol. After the intervention, the rats were tested to behavioral measures such as learning and memory, and the volume and H-spectrum of the brain were measured using MRI; hippocampal dendritic spines were measured using Golgi staining and laser confocal. RESULTS: The results showed that Shank3-deficient rats had significant deficits in social memory, object recognition, and water maze learning decreased hippocampal volume and number of neurons, and lower levels of related scaffold proteins and receptor proteins were found in Shank3-deficient rats. CONCLUSION: It is suggested that early swimming exercise has a positive effect on Shank3 gene-deficient rats, which provides a new therapeutic strategy for the prevention and recovery of neurodevelopmental disorders.


Subject(s)
Autism Spectrum Disorder , Autistic Disorder , Physical Conditioning, Animal , Animals , Rats , Autism Spectrum Disorder/genetics , Autism Spectrum Disorder/therapy , Autistic Disorder/genetics , Autistic Disorder/therapy , Behavior, Animal , Mutation , Nerve Tissue Proteins/genetics , Swimming
8.
Anal Methods ; 14(46): 4893-4902, 2022 12 01.
Article in English | MEDLINE | ID: mdl-36420616

ABSTRACT

In order to effectively remove grease for the detection of sulfonamides, a non-toxic and low-cost hierarchical porous zirconia material was synthesized using the dual template method. The lipid impurities in an animal-derived food matrix can be absorbed by hierarchical zirconia. A ZrO2 prepolymer was synthesized by mixing amphiphilic triblock copolymer poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (P123) with tannin extract as the double template and Zr(SO4)2 as the metal source. After aging, drying and calcination at high temperature, the prepolymer transforms into a hierarchical porous structure. The synthesized materials were characterized using SEM, XRD, FT-IR, and BET. The results show that the material has an abundant pore structure and hierarchical pore structure. The adsorption conditions were optimized. The hierarchical porous ZrO2 synthesized by this method is relatively uniform, and is characterized by large specific surface area as well as high lipid impurity adsorption capacity. Through the optimization experiment of adsorption conditions, we found that hierarchical porous ZrO2 can reach the maximum adsorption capacity in 60 min under weak acidic conditions. The samples are used for actual sample testing such as HPLC of sulfadiazine (SD), sulfamethazine (SM2), sulfamethoxydiazine (SMD), sulfamethoxazole (SIZ) and sulfadimethoxine (SDM), and the recovery experiment of sulfonamides in chicken was carried out. The recoveries were 80.9-97.6% and the detection limit was 3.8-17.6 µg L-1. This work provides a new strategy for oil removal using hierarchical porous materials.


Subject(s)
Polyethylene Glycols , Sulfonamides , Animals , Porosity , Spectroscopy, Fourier Transform Infrared , Sulfanilamide , Lipids
9.
RSC Adv ; 12(33): 21332-21339, 2022 Jul 21.
Article in English | MEDLINE | ID: mdl-35975086

ABSTRACT

Iron is the main substance for maintaining life. Real-time determination of ferric ion (Fe3+) in living cells is of great significance for understanding the relationship of Fe3+ concentration changes with various physiological and pathological processes. Fluorescent probes are suitable for the detection of trace metal ions in cells due to their low toxicity and high sensitivity. In this work, a boron-dipyrromethene-based fluorescent probe (BODIPY-CL) for selective detection of Fe3+ was synthesized. The fluorescence emission of BODIPY-CL was determined at 516 nm. In a pH range of 1 to 10, the probe BODIPY-CL exhibits a quenching response to Fe3+. Meanwhile, BODIPY-CL showed a highly selective response to Fe3+ compared with 16 kinds of metal ions. The stoichiometry ratio of BODIPY-CL bound to Fe3+ was nearly 2 : 1. The fluorescence quenching response obtained by the sensor was linear with the Fe3+ concentration in the range of 0-400 µM, and the detection limit was 2.9 µM. BODIPY-CL was successfully applied to image Fe3+ in cells. This study provides a promising fluorescent imaging probe for further research on the physiological and pathological effects of Fe3+.

10.
Chemosphere ; 249: 126552, 2020 Jun.
Article in English | MEDLINE | ID: mdl-32217414

ABSTRACT

Microsorum pteropus has been proven to be a potential novel aquatic Cd hyperaccumulator. In this study, Non-invasive Micro-test Technology (NMT) was used to observe the ion fluxes of different M. pteropus tissues under Cd exposure. M. pteropus can hyperaccumulate more than 1000 mg/kg Cd in roots and leaves and approximately 600 mg/kg Cd in stems after seven days of exposure to 500 µM Cd, showing that this plant have a great capacity for Cd enrichment and resistance. The NMT test found H+ fluxes increased in all tissues after Cd exposure, with the largest increases being observed in stems, followed by the leaves and roots. Cd2+ fluxes showed different accumulation levels in different tissues, with low-level Cd exposure leading to influxes into roots and leaves, and high-level Cd exposure resulting in effluxes from roots. No significant influxes or effluxes were observed in leaves under high-level Cd exposure, or in stems under low- and high-levels of Cd exposure. However, transient high-level Cd exposure showed long-term Cd2+ influxes into roots and short-term Cd2+ effluxes out of stems and leaves. The roots of M. pteropus had greater regulation mechanisms for Cd enrichment and resistance, with influxes occurring following low-level exposure and effluxes occurring from high-level exposure. When exposed to Cd, M. pteropus stems showed less transportation and absorption. Low-level Cd exposure resulted in individual leaves directly absorbing Cd from hydroponic solutions. Different Cd enrichment and resistance mechanisms were exhibited by different M. pteropus tissues.


Subject(s)
Cadmium/toxicity , Polypodiaceae/physiology , Soil Pollutants/toxicity , Animals , Biological Transport , Chiroptera , Hydroponics , Plant Leaves/physiology , Plant Roots
11.
Plant Mol Biol ; 102(3): 239-252, 2020 Feb.
Article in English | MEDLINE | ID: mdl-31832900

ABSTRACT

KEY MESSAGE: Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peony flowers. To explore the factors underlying inflorescence stem bending, major cell walls contents were measured, and stem structure was observed in two herbaceous peony varieties with contrasting stem straightness traits ('Da Fugui', upright; 'Chui Touhong', bending). In addition, Illumina sequencing was performed and weighted correlation network analysis (WGCNA) was used to analyze the results. The results showed significant differences in lignin, hemicellulose and soluble sugar contents, sclerenchyma and xylem areas and thickening in cell walls in pith at stage S3, when bending begins. In addition, 44,182 significantly differentially expressed genes (DEGs) were found, and these DEGs were mainly enriched in 36 pathways. Among the DEGs, hub genes involved in lignin, cellulose, and xylan biosynthesis and transcription factors that regulated these process were identified by WGCNA. These results suggested that the contents of compounds that provided cell wall rigidity were vital factors affecting inflorescence stem straightness in herbaceous peony. Genes involved in or regulating the biosynthesis of these compounds are thus important; lignin and hemicellulose are of great interest, and cellulose and glycan should not be ignored. This paper lays a foundation for developing new herbaceous peony varieties suitable for cut flowers by molecular-assisted breeding.


Subject(s)
Inflorescence/metabolism , Paeonia/metabolism , Transcriptome , Carbohydrate Metabolism , Cell Wall , Cellulose/metabolism , Flowers , Gene Expression Regulation, Plant , Genes, Plant/genetics , Lignin/metabolism , Paeonia/genetics , Plant Stems/cytology , Plant Stems/growth & development , Polysaccharides
12.
Environ Pollut ; 248: 1020-1027, 2019 May.
Article in English | MEDLINE | ID: mdl-31091634

ABSTRACT

Microsorum pteropus is a novel potential Cd (cadmium) aquatic hyperaccumulator. In the present study, hydroponic experiments were conducted to assess the accumulation and subcellular distribution of Cd in the root, stem and leaf of M. pteropus. SEM (scanning electron microscopy) - EDX (energy dispersive X-ray fluorescence spectrometer) and TEM (transmission electron microscopy) were used to observe the ultrastructure of different tissues under 500 µM Cd exposure. After exposure to 500 µM Cd for 7 days, the root, stem and leaf of M. pteropus can accumulate to be > 400 mg/kg Cd in dry mass with no significant influence on the growth. In the root and leaf of M. pteropus, the Cd was more likely to store in the cell wall fraction. However, Cd in the stem was mainly stored in both the cell wall fraction and the cytoplasm fraction. Under SEM observation and EDX detection, 1) Cd was found to be sequestrated in the epidermis or chelated in the root cells, 2) no significant deposit spots were observed in the stem, 3) Cd was found in the trichome of the leaf, and the sporangium was not damaged. TEM observations revealed 1) possible Cd precipitations in the root cell and 2) no significant ultrastructure variation in the stem, and 3) the chloroplast retained its structure and was not affected by the Cd. M. pteropus showed great capacity for Cd accumulation without influencing growth. In addition, the ultrastructure of all the tissues was not damaged by the Cd. M. pteropus showed a great potential in phytoremediation in heavy metal polluted water solutions, and may provide new directions for the study of resistance mechanisms of aquatic hyperaccumulators.


Subject(s)
Biodegradation, Environmental , Cadmium/analysis , Plant Leaves/chemistry , Plant Roots/chemistry , Plant Stems/chemistry , Polypodiaceae/metabolism , Soil Pollutants/analysis , Chloroplasts/metabolism , Hydroponics , Plant Leaves/growth & development , Plant Roots/growth & development , Plant Stems/growth & development
13.
Sensors (Basel) ; 19(24)2019 Dec 16.
Article in English | MEDLINE | ID: mdl-31888173

ABSTRACT

The linear spectral emissivity constraint (LSEC) method has been proposed to separate temperature and emissivity in hyperspectral thermal infrared data with an assumption that land surface emissivity (LSE) can be described by an equal interval piecewise linear function. This paper combines a pre-estimate shape method with the LSEC method to provide an initial-shape estimation of LSE which will create a new piecewise scheme for land surface temperature (LST) and LSE separation. This new scheme is designated as the pre-estimate shape (PES)-LSEC method. Comparisons with the LSEC method using simulated data sets show that the PES-LSEC method has better performance in terms of accuracy for both LSE and LST. With an at-ground error of 0.5 K, the root-mean-square errors (RMSEs) of LST and LSE are 0.07 K and 0.0045, respectively, and with the scale factor of moisture profile 0.8 and 1.2, the RMSEs of LST are 1.11 K and 1.14 K, respectively. The RMSEs of LSE in each channel are mostly below 0.02 and 0.04, respectively, which are better than for the LSEC method. In situ experimental data are adopted to validate our method: The results show that RMSE of LST is 0.9 K and the mean value of LSE accuracy is 0.01. The PES-LSEC method with fewer segments achieves better accuracy than that of LSEC and preserves most of the crest and trough information of emissivity.

14.
Sci Total Environ ; 649: 1209-1223, 2019 Feb 01.
Article in English | MEDLINE | ID: mdl-30308892

ABSTRACT

Microsorum fortunei (M. fortunei), a close relative to the cadmium (Cd) hyperaccumulator Microsorum pteropus, is an epiphytic Polypodiaceae fern with strong antioxidant activity. The Cd-accumulation capacities and Cd-resistance mechanisms of M. fortunei were analyzed in this study by measuring metal contents (Cd, Fe, Mg, Ca, Zn, Mn, K and Na) and chlorophyll fluorescence parameters (Fv/Fm, qN, qP, Y(II), Y(NPQ) and Y(NO)) and by performing an RNA-sequencing analysis. M. fortunei could accumulate up to 2249.10 µg/g DW Cd in roots under a 15-day 1000 µmol/L Cd treatment, with little Cd translocated into the leaves (maximum 138.26 µg/g DW). The M. fortunei leaves could maintain their normal physiological functions with no phytosynthesis damage and few changes in metal contents or differentially expressed genes. M. fortunei roots showed a decrease in Zn concentration, with potential Cd-tolerance mechanisms such as heavy metal transporters, vesicle trafficking and fusion proteins, antioxidant systems, and primary metabolites like plant hormones, revealed by differentially expressed functional genes. In conclusion, M. fortunei may serve as a potential cadmium-hypertolerant fern that sequesters and detoxifies most cadmium in the roots, with a minimum root-to-shoot Cd translocation to guarantee the physiological functions in the more vulnerable leaves.


Subject(s)
Cadmium/metabolism , Plant Proteins/metabolism , Polypodiaceae/metabolism , Soil Pollutants/metabolism , Transcriptome , China , Plant Leaves/drug effects , Plant Leaves/metabolism , Polypodiaceae/drug effects , Transcriptome/drug effects
15.
Sci Total Environ ; 642: 1369-1377, 2018 Nov 15.
Article in English | MEDLINE | ID: mdl-30045517

ABSTRACT

Microsorum pteropus is a fully or partially submerged Polypodiaceae fern that has been proven to be a potential Cd aquatic hyperaccumulator. Proteomic analysis was used in this study to investigate the resistance mechanisms of M. pteropus root and leaf tissues under Cd stress. M. pteropus plants were exposed to up to 500 µM Cd in hydroponics for 7 days. The plant can accumulate >4,000 mg/kg Cd in both root and leaf dry mass. Meanwhile, the proteins in roots and leaves in the 500 µM Cd treatment were separated and analyzed by proteomics. Eight proteins with altered expression in roots and twenty proteins with altered expression in leaves were identified using MALDI-TOF/TOF-MS (matrix-assisted laser desorption/ionization time of flight mass spectrometry) in this study. The proteins were involved in energy metabolism, antioxidant activity, cellular metabolism and protein metabolism. However, just three proteins were significantly differentially expressed in both tissues, and they were all involved in basal metabolism, indicating different resistance mechanisms between roots and leaves. Root tissues of M. pteropus mainly resist Cd damage by antioxidants and the enhancement of energy metabolism, while leaf tissues of M. pteropus mainly protect themselves by maintaining photosynthetic functions and the regulation of cellular metabolism.


Subject(s)
Cadmium/toxicity , Ferns/physiology , Soil Pollutants/toxicity , Animals , Biodegradation, Environmental , Cadmium/analysis , Chiroptera , Ferns/drug effects , Photosynthesis , Plant Leaves , Plant Roots , Proteomics , Soil Pollutants/analysis
16.
Environ Sci Pollut Res Int ; 25(13): 12507-12514, 2018 May.
Article in English | MEDLINE | ID: mdl-29464599

ABSTRACT

To better monitor and remediate environments contaminated by cadmium (Cd), plants are used as hyperaccumulators or biomonitors; however, few have been identified for aquatic Cd pollution. In our study, two aquatic ornamental plants, Microsorum pteropus (Blume) Copel. and Echinodorus grisebachii Small, were studied for their Cd accumulation capacity, morphological characteristics, and leaf physiological indexes. Microsorum pteropus (Blume) Copel. leaf has the potential to hyperaccumulate Cd (166 mg/kg dry weight for 1 mg/L exposure), with no significant physiological difference under exposure. Echinodorus grisebachii Small had sensitive diagnostic responses to Cd toxicity, such as significant decreases in Chl (a + b) and Chl-a/b, increased peroxidase (POD) activity, greater malondialdehyde (MDA) content, and increased soluble sugar content. These results suggest that Microsorum pteropus (Blume) Copel. could have the potential to be a Cd hyperaccumulator, while Echinodorus grisebachii Small could serve as a biomonitor for Cd-contaminated water bodies.


Subject(s)
Alismataceae/metabolism , Cadmium/metabolism , Polypodiaceae/metabolism , Soil Pollutants/metabolism , Alismataceae/anatomy & histology , Alismataceae/physiology , Biodegradation, Environmental , Plant Leaves/physiology , Polypodiaceae/anatomy & histology , Polypodiaceae/physiology
17.
Sci Total Environ ; 616-617: 480-490, 2018 Mar.
Article in English | MEDLINE | ID: mdl-29127802

ABSTRACT

Microsorum pteropus (M. pteropus), an aquatic Polypodiaceae fern, was identified as a novel potential cadmium (Cd) hyperaccumulator in our previous study. This study reveals the Cd-resistance mechanisms and their difference between the root and leaf of M. pteropus based on analyses of photosynthesis, antioxidant systems and gene expression. A high level of Cd at 500µM was used to treat the samples to test the effects of this compound. Superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA) and flavonoids were used as indicators for antioxidant system changes. Five chlorophyll fluorescent parameters including the maximal photochemical efficiency of photosystem II (Fv/Fm), effective quantum yield of photosystem II (Y(II)), photochemical quenching (qP), nonphotochemical quenching (qN) and electron transport rate (ETR) were measured to determine the photosynthetic changes. RNA-sequencing analysis was used to study the changes in gene expression. The results showed that after exposure to high levels of Cd, the concentrations of enzymatic oxidants (SOD and POD) were significantly increased, while the MDA levels were significantly decreased. There were no significant changes for the chlorophyll fluorescent parameters during Cd stress, which indicates that M. pteropus is highly effective at protecting itself. Certain functional genes, including photosystem genes and secondary metabolites, had significantly altered levels of expression. Different Cd-resistance mechanisms were found between the root and leaf tissues of M. pteropus. The root tissues of M. pteropus resist Cd damage using antioxidants, while its leaf tissues mainly protect themselves using photosystem self-protection.


Subject(s)
Cadmium/metabolism , Plant Leaves/physiology , Plant Roots/physiology , Polypodiaceae/physiology , Antioxidants/physiology , Chlorophyll , Gene Expression Regulation, Plant , Photosynthesis
19.
Environ Pollut ; 219: 883-896, 2016 Dec.
Article in English | MEDLINE | ID: mdl-27613328

ABSTRACT

The temporal-spatial distributions of DDT-related contaminants (DDXs), including DDT (dichlorodiphenyltrichloroethane), DDE (dichlorodiphenyldichloroethylene) and DDD (dichlorodiphenyldichloroethane), in the sediments of Lake Chaohu and their influencing factors were studied. p,p-DDE and p,p-DDD were found to be the two dominant components of DDXs in both surface and core sediments. The parent DDT compounds were still detectable in sediment cores after the late 1930s. Historical usage of technical DDT was identified as the primary source of DDXs in sediments, as indicated by DDT/(DDD + DDE) ratios of less than one. The residual levels of DDXs were higher in the surface and core sediments in the western lake area than in other lake areas, which might be due to the combined inflow effects of municipal sewage, industrial wastewater and agricultural runoff. The DDX residues in the sediment cores reached peak values in the late 1970s or early 1980s. There were significant positive relationships between DDX residues in sediment cores with annual DDT production and with fine particulate sizes (<4.5 µm). The relationship between the DDXs and TOC in sediment was complex, as indicated by the significant differences among the surface and core sediments. The algae-derived organic matter significantly influenced the amount of residue, composition and distribution of DDXs in the sediments. The DDD/DDE ratios responded well to the anaerobic conditions in the sediments that were caused by algal blooms after the late 1970s in the western lake area. This suggests that the algae-derived organic matter was an important factor and served as a biomarker of eutrophication and also affected the DDX residues and lifecycle in the lake ecosystem.


Subject(s)
DDT/analogs & derivatives , DDT/analysis , Geologic Sediments/chemistry , Hydrocarbons, Chlorinated/analysis , Lakes/chemistry , Water Pollutants, Chemical/analysis , China , Environmental Monitoring , Eutrophication
20.
Environ Pollut ; 219: 724-734, 2016 Dec.
Article in English | MEDLINE | ID: mdl-27427173

ABSTRACT

In the shallow lakes, the partitioning of organic contaminants into the water phase from the solid phase might pose a potential hazard to both benthic and planktonic organisms, which would further damage aquatic ecosystems. This study determined the concentrations of polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs), and phthalate esters (PAEs) in both the sediment and the pore water from Lake Chaohu and calculated the sediment - pore water partition coefficient (KD) and the organic carbon normalized sediment - pore water partition coefficient (KOC), and explored the effects of particle size, organic matter content, and parallel factor fluorescent organic matter (PARAFAC-FOM) on KD. The results showed that log KD values of PAHs (2.61-3.94) and OCPs (1.75-3.05) were significantly lower than that of PAEs (4.13-5.05) (p < 0.05). The chemicals were ranked by log KOC as follows: PAEs (6.05-6.94) > PAHs (4.61-5.86) > OCPs (3.62-4.97). A modified MCI model can predict KOC values in a range of log 1.5 at a higher frequency, especially for PAEs. The significantly positive correlation between KOC and the octanol - water partition coefficient (KOW) were observed for PAHs and OCPs. However, significant correlation was found for PAEs only when excluding PAEs with lower KOW. Sediments with smaller particle sizes (clay and silt) and their organic matter would affect distributions of PAHs and OCPs between the sediment and the pore water. Protein-like fluorescent organic matter (C2) was associated with the KD of PAEs. Furthermore, the partitioning of PARAFAC-FOM between the sediment and the pore water could potentially affect the distribution of organic pollutants. The partitioning mechanism of PAEs between the sediment and the pore water might be different from that of PAHs and OCPs, as indicated by their associations with influencing factors and KOW.


Subject(s)
Geologic Sediments/chemistry , Organic Chemicals/analysis , Organic Chemicals/chemistry , Water Pollutants, Chemical/analysis , Water Pollutants, Chemical/chemistry , Hydrocarbons, Chlorinated/analysis , Hydrocarbons, Chlorinated/chemistry , Lakes/chemistry , Particle Size , Polycyclic Aromatic Hydrocarbons/analysis , Polycyclic Aromatic Hydrocarbons/chemistry , Solubility
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