ABSTRACT
The vocal fold is formed by tissues with abundant cells surrounded by an extracellular matrix. One of the most important components of the extracellular matrix is hyaluronic acid (HA). This study evaluated the inflammatory response of the vocal folds of rabbits submitted to local injection of Restylane HA. We performed a prospective, experimental, and controlled study in 22 New Zealand rabbits with injection in vocal fold. The 22 adult male rabbits underwent a 0.1-ml injection of Restylane HA in one vocal fold and a 0.1-ml injection of saline solution in the other vocal fold, at random. The animals were then subdivided in two distinct groups. One group was sacrificed after a 1-week follow-up and the other group after 3 months. Slides were then stained with hematoxylin-eosin (HE), Masson's trichrome, and toluidine blue. HA was found microscopically in all specimens of both groups. Using the stain (HE), it was easily distinguished. We have measured the inflammatory reaction by histopathology conducted by a pathologist. An increase in connective tissue was found surrounding the HA, and was always associated with a mild inflammatory response. The extended time of exposure did not increase the intensity of the inflammatory reaction. Tissue necrosis and foreign body inflammatory reaction were not observed in either group. The current study suggests that HA is a good alternative for a filling material of the vocal folds when treating glottal insufficiency.
Subject(s)
Hyaluronic Acid/analogs & derivatives , Laryngitis/chemically induced , Vocal Cords/drug effects , Animals , Fibrosis , Hyaluronic Acid/pharmacology , Laryngitis/pathology , Male , Necrosis , Neovascularization, Physiologic , Rabbits , Vocal Cords/pathologyABSTRACT
O papilomavírus humano (HPV) é universalmente aceito como agente causal do câncer de colo uterino e, recentemente, vem se especulando sobre sua possível relação com câncer oral e de orofaringe. O carcinoma espinocelular (CEC) oral representa 90 por cento de todos os tumores malignos que afetam a cavidade bucal. Estudos sobre a prevalência de HPV em pacientes com CEC variam de 0 a 100 por cento. O efeito citopático viral mais conhecido é a coilocitose, considerado "critério maior" na infecção pelo HPV do ponto de vista histopatológico. OBJETIVO: O objetivo deste estudo foi verificar a prevalência de achados sugestivos de HPV - coilocitose - em CEC oral e de orofaringe. FORMA DE ESTUDO: coorte transversal. MATERIAL E MÉTODO: Foram examinadas no microscópio 20 lâminas com o diagnóstico de CEC de cavidade oral ou orofaringe sendo que em 15 delas foi encontrada coilocitose, correspondendo a 75 por cento. RESULTADO: Apesar de termos conhecimento que o método com maior sensibilidade atual para pesquisa de HPV ser a reação de polimerase em cadeia (PCR), iniciamos esta pesquisa com a investigação de coilocitose, o que é muito sugestivo de infecção por HPV. CONCLUSÃO: O estudo em questão trata-se de um projeto-piloto pois será dada continuidade a esta pesquisa através da realização de PCR a fim de confirmar a alta prevalência de infecção por HPV em CEC oral e de orofaringe.
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged, 80 and over , Carcinoma, Squamous Cell/pathology , Papillomavirus Infections/pathology , Mouth Neoplasms/pathology , Oropharyngeal Neoplasms/pathology , Papillomaviridae , Biopsy , Carcinoma, Squamous Cell/virology , Papillomavirus Infections/complications , Oropharyngeal Neoplasms/virologyABSTRACT
UNLABELLED: Human papillomavirus (HPV) is considered to be an etiologic agent of cervical cancer and, recently its relation to oral and oropharyngeal cancer has also been investigated. Oral squamous cell carcinoma (SCC) represents 90% of all malignant tumors that affect the oral cavity. The prevalence of HPV in patients with SCC ranges from 0 to 100%. The most known viral cytopathic effect is koilocytosis, considered to be a major characteristic of HPV infection. AIM: The aim of this study was to verify the prevalence of some peculiar characteristics of HPV--koilocytosis--in oral and oropharyngeal SCC. STUDY DESIGN: Transversal cohort. MATERIAL AND METHOD: Twenty slides with oral and/or oropharyngeal SCC were examined under microscopy. RESULTS: In 15 of them, koilocytosis was found, amounting to 75%. Although we know that polymerase chain reaction (PCR) is the method with the best sensitivity for HPV detection, we began this research looking for koilocytosis, which is highly suggestive of HPV infection. CONCLUSION: This study is a trial project and we will continue this research with PCR measures to confirm this high prevalence of HPV infection in oral and oropharyngeal SCC.
Subject(s)
Carcinoma, Squamous Cell/pathology , Mouth Neoplasms/pathology , Oropharyngeal Neoplasms/pathology , Papillomaviridae , Papillomavirus Infections/pathology , Adult , Aged , Aged, 80 and over , Biopsy , Carcinoma, Squamous Cell/virology , Female , Humans , Male , Middle Aged , Oropharyngeal Neoplasms/virology , Papillomavirus Infections/complicationsABSTRACT
Os autores relatam um raro caso de criança portadora de anomalia originada da primeira fenda cervial cervical. Ela apresentava otorréia no ouvido esquerdo, em concomitância com a presença de um sínus servical. Após drenagem cirúrgica para tratamento do abscesso no triángulo anterior do pescoço, surgiu fístula que se manteve e colocou em comunicaçäo a pele com o assoalho do conduto auditovo externo. Comentam ainda a etiologia, incidência e tratamento cirúrgico
Subject(s)
Humans , Female , Infant , Fistula , Branchial Region/abnormalities , Fistula/complications , Fistula/pathology , Fistula/surgery , Microcephaly/complications , Branchial Region/surgery , SuppurationABSTRACT
É apresentado um caso de uma paciente de 33 anos de idade, com história de amenorréia e galactorréia, com hiperprolactinemia. Radiologicamente evidenciou-se presença de tumor hipofisário (macroprolactinoma). Ao exame anatomopatológico de esvaziamento selar e para-selar diagnosticou-se carcinoma hipofisário. Analisam-se as controvérsias e dificuldades diagnósticas dessa rara entidade nosológica
Subject(s)
Adult , Humans , Female , Pituitary Neoplasms/diagnosis , Pituitary Neoplasms/pathologyABSTRACT
Registro de um caso da doença de Creutzfeldt-Jakob, forma de Heidenhain, que se caracteriza por comprometimento visual importante. As manifestaçöes iniciais consistiram de distúrbios visuais. A paciente evoluiu para franca demência, ao lado de manifestaçöes pirâmido-extrapiramidais. Além dos dados clínicos o EEG mostrou atividade periódica característica da doença. O estudo anátomo-patológico confirmou o diagnóstico clínico mostrando alteraçäo, esponjosa, degeneraçäo neuronal e astrogliose. Além do quadro clínico, eletrencefalográfico e anátomo-patológico säo discutidos os aspectos etiopatogênicos da doença
