Diagnosis of suspected trimethylaminuria by NMR spectroscopy.

BACKGROUND: Trimethylamine (TMA) is a volatile substance produced in the gut, absorbed into the blood and further metabolized by healthy individuals into trimethylamine-N-oxide (TMAO) by TMA-oxidase and then excreted in urine. Patients suffering from trimethylaminuria (TMAU) show an impaired enzymatic oxidation of TMA, excreting this amine in breath, urine and other body secretions which confers an unpleasant body odor. METHODS: We diagnosed a Brazilian adult male patient suspected of trimethylaminuria with a burden of choline bitartarate by monitoring the urinary excretion of TMA and TMAO by proton nuclear magnetic resonance spectroscopy ((1)H-NMR). RESULTS: The patient's urinalyses showed an augmented TMA (12.64+/-0.95 mg/l) and TMAO (88.42+/-0.82 mg/l) excretion 6 h after the overload test representing an oxidation capacity of 84.6%, consistent with a heterozygosis condition. Diets containing tuna fish or eggs resulted in an excretion of TMA and TMAO similar to that of the control diet. Only the diet based on dogfish, rich in TMAO, enhanced the excretion of TMA and TMAO reaching 24.65 and 1055.55 mg/l, respectively, in the 0-24 h urine sample. CONCLUSIONS: It was concluded first, that the patient was not able to metabolize the dietary overload of TMA and second, that more studies are needed to substantiate foods that should be avoided, especially regarding fish, due to their high TMA precursor contents.

Effect of harvesting and drying conditions on chlorophyll levels of soybean (Glycine max L. Merr).

Chlorophyll in soybean represents a downgrading factor for the crops. Five Brazilian cultivars were harvested between R(6) and R(8) stage of development (Fehr & Caviness scale) and dried at 25 degrees and 40 degrees C. The effect of maturity stages and two drying conditions after harvest were studied to achieve reduction of moisture and chlorophylls to acceptable levels. When seeds were dried at 25 degrees C, even harvesting at early stages of development such as R(6), the green pigments were almost degraded, and 16 ppm of chlorophyll were found at maximum, accompanied by loss of moisture. Moisture and chlorophyll declines as seed matures, but at intermediary stages (R(6)-R(7)), chlorophyll degrades first, so the rate of moisture loss should not be used to predict chlorophyll contents. At 40 degrees C, complete degradation of chlorophyll pigments is only achieved when seeds are swathed from R(7) stage up, otherwise the seed quality could be compromised. Slow drying allows almost complete removal of green pigments, even when seeds are swathed a few days before the physiological maturity stage.

Relationship between color (instrumental and visual) and chlorophyll contents in soybean seeds during ripening.

The correlation between chlorophyll content and quantitative color parameters was investigated in order to find an indirect method for predicting green pigment in ripening soybean seeds. Five Brazilian soybean varieties harvested at different maturity stages (R(6) to R(8) according to the scale of Fehr & Caviness) and dried under two conditions (in oven at 40 degrees C with circulating air and at ambient temperature around 25 degrees C) were analyzed in two consecutive years. The slow-dried seeds at 25 degrees C lost chlorophyll faster, whereas drying at 40 degrees C did not result in yellowing of seeds. High and significant linear correlations between a value and total chlorophyll were obtained over the whole maturation period and on both conditions of drying. From an industrial point of view it appears that a value, obtained by the CIE-L*a*b* method, seems to be a good tool to be applied for quality control and classifying soybean seeds for different purposes.