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1.
Nurs Child Young People ; 28(4): 75-6, 2016 May 09.
Article in English | MEDLINE | ID: mdl-27214445

ABSTRACT

UNLABELLED: Theme: Accreditation and quality improvement. AIM: Dislocated fractures are common in the children's emergency department (ER). All forms of fracture reduction are very painful requiring nitrous oxide. The purpose is to shorten the length of stay in the hospital as well as sustain a high quality of care. METHODS: All nurses received theoretical and practical training in the use of nitrous oxide. Evaluations with the families were made by telephone. RESULT: A total of 40 enclosed fracture reductions were made at the ER, leading to a reduction of 33 patients in the operating department and the length of stay was shortened - this compared to the same time in 2014. No adverse event was reported and no patient felt any increase in pain during the treatment. All patients would repeat the procedure if necessary. CONCLUSION: The treatment has reduced the length of stay in the hospital without affecting the other patients in the ER or the quality of care.


Subject(s)
Fracture Fixation/methods , Nitrous Oxide/pharmacology , Nitrous Oxide/therapeutic use , Pediatrics/methods , Child , Child, Preschool , Emergency Service, Hospital , Humans , Length of Stay/trends , Pediatrics/standards , Prospective Studies
2.
Mol Biol Cell ; 17(7): 2986-95, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16624864

ABSTRACT

The mode of activation of glycoprotein 130 kDa (gp130) and the transmission of the activation status through the plasma membrane are incompletely understood. In particular, the molecular function of the three juxtamembrane fibronectin III-like domains of gp130 in signal transmission remains unclear. To ask whether forced dimerization of gp130 is sufficient for receptor activation, we replaced the entire extracellular portion of gp130 with the c-jun leucine zipper region in the chimeric receptor protein L-gp130. On expression in cells, L-gp130 stimulates ligand-independent signal transducer and activator of transcription (STAT) 3 and extracellular signal-regulated kinase 1/2 phosphorylation. gp130 activation could be abrogated by the addition of a competing peptide comprising the leucine zipper region of c-fos. When stably expressed in the interleukin-3-dependent Ba/F3 murine pre-B-cells, these cells showed constitutive STAT3 activation and cytokine-independent growth over several months. Because gp130 stimulation completely suppressed differentiation of murine embryonic stem cells in vitro, we also stably expressed L-gp130 in these cells, which completely blocked their differentiation in the absence of cytokine stimulation and was consistent with high constitutive expression levels of the stem cell factor OCT-4. Thus, L-gp130 can be used in vitro and in vivo to mimic constitutive and ligand-independent activation of gp130 and STAT3, the latter of which is frequently observed in neoplastic diseases.


Subject(s)
Cell Differentiation , Cytokine Receptor gp130/metabolism , Leucine Zippers , STAT3 Transcription Factor/agonists , Stem Cells/cytology , Animals , Cell Differentiation/genetics , Cytokine Receptor gp130/agonists , Cytokine Receptor gp130/genetics , Cytokines/metabolism , Dimerization , Humans , Interleukin-6/metabolism , Leucine Zippers/genetics , Mice , Protein Structure, Tertiary/genetics , Proto-Oncogene Proteins c-jun/genetics , Recombinant Fusion Proteins/agonists , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , STAT3 Transcription Factor/metabolism , Stem Cells/metabolism , Transcription, Genetic
3.
J Biol Chem ; 279(34): 35542-50, 2004 Aug 20.
Article in English | MEDLINE | ID: mdl-15201271

ABSTRACT

Spinocerebellar ataxia (SCA) type 10, an autosomal dominant disease characterized by cerebellar ataxia, is caused by a novel pentanucleotide (ATTCT) repeat expansion in the SCA10 gene. Although clinical features of the disease are well characterized, nothing is known so far about the affected SCA10 gene product, ataxin-10 (Atx-10). We have cloned the rat SCA10 gene and expressed the corresponding protein in HEK293 cells. Atx-10 has an apparent molecular mass of approximately 55 kDa and belongs to the family of armadillo repeat proteins. In solution, it tends to form homotrimeric complexes, which associate via a tip-to-tip contact with the concave sides of the molecules facing each other. Atx-10 immunostaining of mouse and human brain sections revealed a predominantly cytoplasmic and perinuclear localization with a clear restriction to olivocerebellar regions. Knock down of SCA10 in primary neuronal cells by small interfering RNAs resulted in an increased apoptosis of cerebellar neurons, arguing for a loss-of-function phenotype in SCA10 patients.


Subject(s)
Cerebellum/pathology , Nerve Tissue Proteins/genetics , Spinocerebellar Ataxias/genetics , Amino Acid Sequence , Animals , Apoptosis/genetics , Ataxin-10 , Base Sequence , Cell Survival/genetics , Cells, Cultured , Cerebellum/metabolism , Down-Regulation , Humans , Mice , Molecular Sequence Data , Rats , Rats, Wistar , Repetitive Sequences, Nucleic Acid , Spinocerebellar Ataxias/etiology , Spinocerebellar Ataxias/pathology , Spinocerebellar Degenerations/etiology , Spinocerebellar Degenerations/genetics , Tissue Distribution
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