Bacteriohopanepolyols along redox gradients in the Humboldt Current System off northern Chile.

Marine oxygen minimum zones (OMZs) are characterized by the presence of subsurface suboxic or anoxic waters where diverse microbial processes are responsible for the removal of fixed nitrogen. OMZs have expanded over past decades and are expected to continue expanding in response to the changing climate. The implications for marine biogeochemistry, particularly nitrogen cycling, are uncertain. Cell membrane lipids (biomarkers), such as bacterial bacteriohopanepolyols (BHPs) and their degradation products (hopanoids), have distinctive structural attributes that convey information about their biological sources. Since the discovery of fossil hopanoids in ancient sediments, the study of BHPs has been of great biogeochemical interest due to their potential to serve as proxies for bacteria in the geological record. A stereoisomer of bacteriohopanetetrol (BHT), BHT II, has been previously identified in OMZ waters and has as been unequivocally identified in culture enrichments of anammox bacteria, a key group contributing to nitrogen loss in marine OMZs. We tested BHT II as a proxy for suboxia/anoxia and anammox bacteria in suspended organic matter across OMZ waters of the Humboldt Current System off northern Chile, as well as in surface and deeply buried sediments (125-150 ky). The BHT II ratio (BHT II/total BHT) increases as oxygen content decreases through the water column, consistent with previous results from Perú, the Cariaco Basin and the Arabian Sea, and in line with microbiological evidence indicating intense anammox activity in the Chilean OMZ. Notably, BHT II is transported from the water column to surface sediments, and preserved in deeply buried sediments, where the BHT II ratio correlates with changes in δ15 N sediment values during glacial-interglacial transitions. This study suggests that BHT II offers a proxy for past changes in the relative importance of anammox, and fluctuations in nitrogen cycling in response to ocean redox changes through the geological record.

Developing human capital for successful implementation of international marine scientific research projects.

The oceans play a crucial role in the global environment and the sustainability of human populations, because of their involvement in climate regulation and provision of living and non-living resources to humans. Maintenance of healthy oceans in an era of increasing human pressure requires a high-level understanding of the processes occurring in the marine environment and the impacts of anthropogenic activities. Effective protection and sustainable resource management must be based, in part, on knowledge derived from successful research. Current marine research activities are being limited by a need for high-quality researchers capable of addressing critical issues in broad multidisciplinary research activities. This is particularly true for developing countries which will require the building of capacity for marine scientific research. This paper reviews the current activities aimed at increasing marine research capacity in developing and emerging countries and analyses the challenges faced, including: appropriate alignment of the research goals and societal and policy-relevant needs; training in multidisciplinary research; increasing capacity for overall synthesis of scientific data; building the capacity of technical staff; keeping highly qualified personnel in marine scientific research roles; cross-cultural issues in training; minimising duplication in training activities; improving linkages among human capital, project resources and infrastructure. Potential solutions to these challenges are provided, along with some priorities for action aimed at improving the overall research effort.

Historical overfishing and the recent collapse of coastal ecosystems.

Ecological extinction caused by overfishing precedes all other pervasive human disturbance to coastal ecosystems, including pollution, degradation of water quality, and anthropogenic climate change. Historical abundances of large consumer species were fantastically large in comparison with recent observations. Paleoecological, archaeological, and historical data show that time lags of decades to centuries occurred between the onset of overfishing and consequent changes in ecological communities, because unfished species of similar trophic level assumed the ecological roles of overfished species until they too were overfished or died of epidemic diseases related to overcrowding. Retrospective data not only help to clarify underlying causes and rates of ecological change, but they also demonstrate achievable goals for restoration and management of coastal ecosystems that could not even be contemplated based on the limited perspective of recent observations alone.

Upwelling intensification as part of the Pliocene-Pleistocene climate transition.

A deep-sea sediment core underlying the Benguela upwelling system off southwest Africa provides a continuous time series of sea surface temperature (SST) for the past 4.5 million years. Our results indicate that temperatures in the region have declined by about 10 degrees C since 3.2 million years ago. Records of paleoproductivity suggest that this cooling was associated with an increase in wind-driven upwelling tied to a shift from relatively stable global warmth during the mid-Pliocene to the high-amplitude glacial-interglacial cycles of the late Quaternary. These observations imply that Atlantic Ocean surface water circulation was radically different during the mid-Pliocene.

Corticosteroid receptors in liver cytosol of the clawed toad, Xenopus laevis: influence of thyroid and ovarian hormones.

The glucocorticoid receptor capacity Ro and the dissociation constant Kd were determined in the liver of Xenopus laevis by Scatchard analysis. In 5-year-old female toads Ro was about three times higher than that in males (153.9, 54.3 fmol/mg protein) and Kd was similar in both sexes (4.0, 4.1 nM). Some of the animals used had abnormal enlarged thyroid glands, atrophic ovaries, or both defects in connection with different levels of Ro, but not of Kd, compared to those of normal animals. Females with ovarian atrophy showed significantly lower Ro values, in the same range as in normal males, and a high liver weight. In male and female toads with enlarged thyroid glands and in animals with both defects a significantly higher Ro occurred compared to that of the corresponding group without this abnormality. To study the influence of thyroid hormones on glucocorticoid receptors, young toads (2-3 years old) received injections of 4-phenyl-2-thiouracil, T3, or T4 on 7 consecutive days. Ro and Kd were determined on the following day. Doses of 50 and 500 ng T3 and of 500 and 5000 ng T4 per gram of body weight and day resulted in an increase of Ro up to 250% of the controls. Injections of T3 were more efficient in males than in females. The effect of thyroxine was about the same in both sexes. These observations suggest that thyroid and ovarian hormones exert an influence on glucocorticoid receptor capacity and may belong to the factors which regulate glucocorticoid receptors.

Corticosteroid receptors in liver cytosol of the clawed toad, Xenopus laevis: daily and seasonal variations.

In postmetamorphic Xenopus laevis liver cytosol the glucocorticoid binding capacity R0 and the dissociation constant Kd were determined. The receptor assay included an incubation period of 24 hr at 0-4 degrees with sodium molybdate to stabilize the receptor. Dexamethasone, triamcinolone acetonide, and corticosterone as tritiated ligands were compared regarding the R0 (67.6, 57.2, and 30.7 fmol/mg protein), the Kd (3.54, 0.56, and 9.03 nM), and the rate of dissociation in young specimens of X. laevis. In adult toads the [3H]dexamethasone receptor binding capacity was threefold higher in females than in males (153.86 +/- 12.19, 54.29 +/- 4.5 fmol/mg protein)--with about the same Kd (3.97 +/- 0.57, 4.08 +/- 0.28 nM). Young toads were kept under an artificial light regime (light from 600 to 1800 hr) and dexamethasone binding was measured every 3 hr. Unlike Kd, R0 showed a significant diurnal variation with maximal values at 600 and 1800 hr, which occurred about 9 hr after a maximal level of corticosterone in serum was reached (900, 2100). Seasonal variations of the [3H]dexamethasone and [3H]corticosterone binding capacity were different in both sexes of adult X. laevis. Maximal values in males were found in June/July and October/November. In females, the R0 was increased in the second half of the year with the maximum in August (275.5 +/- 45.02 fmol/mg protein). No correlation between R0 and the concentrations of corticosterone or aldosterone existed.