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1.
Neurosci Lett ; 329(3): 354-8, 2002 Sep 06.
Article in English | MEDLINE | ID: mdl-12183047

ABSTRACT

Cyclooxygenases (COX), key enzymes in prostanoid biosynthesis, may represent important therapeutic targets in various neurodegenerative diseases. In the present study, we explored the role of COX in Parkinson's disease (PD) by using 1-methyl-4-phenyl1, 2, 3, 6-tetrahydropyridine (MPTP) as a tool to create a rodent Parkinsonian model. MPTP (20 mg/kg, subcutaneously) was injected daily into COX-1- and COX-2-deficient mice and wild-type (WT) controls for five consecutive days. Immunocytochemical analysis of tissues collected 7 days after the final MPTP treatment showed that MPTP significantly decreased the number of tyrosine hydroxylase-immunoreactive (TH-ir) neurons in the substantia nigra pars compacta (SNc) of WT (40% decrease) and COX-1(-/-) (45% decrease) mutants. However, a much smaller loss of TH-ir neurons in COX-2(-/-) mutants (20% decrease) was observed. Furthermore, electrochemical analysis revealed a more than 70% decrease in the levels of dopamine and its metabolites (3,4-dihydroxyphenylacetic acid and homovanillic acid) in the striatum of the WT control COX-1(-/-) and COX-2(-/-) mutant mice. These results indicate that loss of COX-2 activity reduces MPTP-induced damage to the dopaminergic neurons of the SNc, but does not alter the levels of dopamine and its metabolites in the striatum. Interestingly, MPTP caused the same degree of loss of dopaminergic neurons in both COX-2(+/-) and COX-2(-/-) mice (20% loss). The results of this study indicate an important role of COX-2 in MPTP-induced neuronal degeneration and suggest the possibility that manipulation of the COX-2 could be an important target for therapeutic interventions in PD.


Subject(s)
Isoenzymes/genetics , MPTP Poisoning/genetics , MPTP Poisoning/pathology , Neurons/pathology , Prostaglandin-Endoperoxide Synthases/genetics , Substantia Nigra/pathology , Animals , Cyclooxygenase 1 , Cyclooxygenase 2 , Dopamine/physiology , Homozygote , Immunity, Innate , Membrane Proteins , Mice , Mice, Mutant Strains , Neurons/enzymology , Substantia Nigra/enzymology , Tyrosine 3-Monooxygenase/metabolism
2.
Circulation ; 104(20): 2453-8, 2001 Nov 13.
Article in English | MEDLINE | ID: mdl-11705824

ABSTRACT

BACKGROUND: The purpose of this study was to examine the effects of cyclooxygenase (COX) deficiency on baseline functional characteristics and on recovery of left ventricular developed pressure (LVDP) after 20 minutes of global ischemia and 40 minutes of reperfusion in untreated and preconditioned hearts. METHODS AND RESULTS: Compared with hearts from wild-type (WT) and COX-2(-/-) mice, baseline cardiac prostaglandin (PG) E(2) and 6-keto-PGF(1alpha) levels were significantly decreased in hearts from COX-1(-/-) mice. After ischemia, cardiac PGE(2) levels increased in WT, COX-1(-/-), and COX-2(-/-) mice (P<0.05). Recovery of function (LVDP) after global ischemia in hearts from COX-1(-/-) and COX-2(-/-) mice was significantly less than in WT hearts. Pretreatment of WT mice with indomethacin for 2 days before ischemia significantly decreased LVDP recovery; however, perfusion of WT hearts with indomethacin for 40 minutes before ischemia did not significantly alter LVDP recovery. Postischemic recovery of LVDP in COX-1(-/-) and COX-2(-/-) was unchanged by perfusion with 5 micromol/L PGE(2), PGD(2), PGF(2alpha), or carboprostacyclin. Hearts from COX-2(-/-) mice showed an increase in ischemic contracture compared with hearts from WT and COX-1(-/-) mice; however, hearts did not differ in intracellular pH, ATP, or inorganic phosphate during ischemia. Ischemic preconditioning significantly improved postischemic LVDP recovery in COX-1(-/-), COX-2(-/-), and WT mice. CONCLUSIONS: Genetic disruption or 2-day chemical inhibition of COX-1 and COX-2 decreases recovery of LVDP after ischemia; however, acute perfusion with indomethacin is not detrimental. These data are consistent with protection due to the altered expression of some protein that is modulated by COX or its metabolites.


Subject(s)
Ischemic Preconditioning, Myocardial , Isoenzymes/genetics , Isoenzymes/physiology , Myocardial Reperfusion Injury/etiology , Prostaglandin-Endoperoxide Synthases/genetics , Prostaglandin-Endoperoxide Synthases/physiology , Animals , Cyclooxygenase 1 , Cyclooxygenase 2 , Cyclooxygenase 2 Inhibitors , Cyclooxygenase Inhibitors/pharmacology , Hemodynamics , Hydrogen-Ion Concentration , Indomethacin/pharmacology , Isoenzymes/antagonists & inhibitors , Kinetics , Membrane Proteins , Mice , Mice, Knockout , Myocardial Contraction , Myocardial Reperfusion Injury/physiopathology , Myocardial Reperfusion Injury/therapy , Organ Culture Techniques , Phosphates/metabolism , Prostaglandins/pharmacology , Ventricular Pressure
3.
Am J Respir Cell Mol Biol ; 25(4): 457-65, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11694451

ABSTRACT

Bacterial lipopolysaccharide (LPS) is a risk factor for exacerbation of asthma and causes airway inflammation. The aim of this study was to examine the effects of disruption of prostaglandin (PG) H synthase (PGHS)-1 and PGHS-2 genes on pulmonary responses to inhaled LPS. PGHS-1(-/-), PGHS-2(-/-), and wild-type (WT) mice were exposed to 4 to 6 microg/m(3) LPS via aerosol. Enhanced pause (PenH), a measure of bronchoconstriction, was assessed using a whole-body plethysmograph before and immediately after a 4-h LPS exposure. Bronchoalveolar lavage (BAL) was performed after LPS exposure to assess inflammatory cells, cytokines/chemokines (tumor necrosis factor-alpha, interleukin-6, and macrophage inflammatory protein-2), and PGE(2). The degree of lung inflammation was scored on hematoxylin-and-eosin-stained sections. PGHS-1 and PGHS-2 protein levels were determined by immunoblotting. All mice exhibited increased PenH and methacholine responsiveness after LPS exposure; however, these changes were much more pronounced in PGHS-1(-/-) and PGHS-2(-/-) mice relative to WT mice (P < 0.05). There were no significant differences in inflammation as assessed by BAL fluid (BALF) cells or lung histology between the genotypes despite reduced BALF cytokines/chemokines and PGE(2) in PGHS-1(-/-) and PGHS-2(-/-) mice relative to WT mice (P < 0.05). PGHS-2 was upregulated more in PGHS-1(-/-) mice compared with WT mice after LPS exposure. We conclude that: (1) airway inflammation and hyperresponsiveness are dissociated in PGHS-1(-/-) and PGHS-2(-/-) mice exposed to LPS; (2) the balance of PGHS-1 and PGHS-2 is important in regulating the functional respiratory responses to inhaled LPS; and (3) neither PGHS-1 nor PGHS-2 is important in regulating basal lung function or the inflammatory responses of the lung to inhaled LPS.


Subject(s)
Isoenzymes/genetics , Lipopolysaccharides/pharmacology , Pneumonia/physiopathology , Prostaglandin-Endoperoxide Synthases/genetics , Administration, Inhalation , Animals , Bronchoalveolar Lavage Fluid , Chemokines/metabolism , Cyclooxygenase 1 , Cyclooxygenase 2 , Cytokines/metabolism , Dinoprostone/biosynthesis , Disease Models, Animal , Female , Isoenzymes/drug effects , Isoenzymes/metabolism , Leukotriene B4/biosynthesis , Lipopolysaccharides/administration & dosage , Lung/pathology , Male , Membrane Proteins , Mice , Mice, Inbred Strains , Mice, Mutant Strains , Pneumonia/chemically induced , Prostaglandin-Endoperoxide Synthases/drug effects , Prostaglandin-Endoperoxide Synthases/metabolism , Proteins/metabolism , Up-Regulation
5.
J Bone Miner Res ; 16(4): 660-70, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11315993

ABSTRACT

Aseptic loosening is a major complication of prosthetic joint surgery and is manifested as chronic inflammation, pain, and osteolysis at the bone implant interface. The osteolysis is believed to be driven by a host inflammatory response to wear debris generated from the implant. In our current study, we use a selective inhibitor (celecoxib) of cyclo-oxygenase 2 (COX-2) and mice that lack either COX-1 (COX-1-/-) or COX-2 (COX-2-/-) to show that COX-2, but not COX-1, plays an important role in wear debris-induced osteolysis. Titanium (Ti) wear debris was implanted surgically onto the calvaria of the mice. An intense inflammatory reaction and extensive bone resorption, which closely resembles that observed in patients with aseptic loosening, developed within 10 days of implantation in wild-type and COX-1-/- mice. COX-2 and prostaglandin E2 (PGE2) production increased in the calvaria and inflammatory tissue overlying it after Ti implantation. Celecoxib (25 mg/kg per day) significantly reduced the inflammation, the local PGE2 production, and osteolysis. In comparison with wild-type and COX-1-/- mice, COX-2-/- mice implanted with Ti had a significantly reduced calvarial bone resorption response, independent of the inflammatory response, and significantly fewer osteoclasts were formed from cultures of their bone marrow cells. These results provide direct evidence that COX-2 is an important mediator of wear debris-induced osteolysis and suggests that COX-2 inhibitors are potential therapeutic agents for the prevention of wear debris-induced osteolysis.


Subject(s)
Isoenzymes/physiology , Osteolysis/enzymology , Prostaglandin-Endoperoxide Synthases/physiology , Prosthesis Failure , Animals , Bone Resorption/etiology , Celecoxib , Cells, Cultured/drug effects , Crosses, Genetic , Cyclooxygenase 1 , Cyclooxygenase 2 , Cyclooxygenase 2 Inhibitors , Cyclooxygenase Inhibitors/pharmacology , Dinoprostone/biosynthesis , Female , Interleukin-1/biosynthesis , Interleukin-6/biosynthesis , Isoenzymes/antagonists & inhibitors , Isoenzymes/deficiency , Isoenzymes/genetics , Macrophage Activation , Membrane Proteins , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Mice, Knockout , Osteoclasts/pathology , Osteolysis/etiology , Osteolysis/pathology , Prostaglandin-Endoperoxide Synthases/deficiency , Prostaglandin-Endoperoxide Synthases/genetics , Prostheses and Implants , Pyrazoles , Skull , Sulfonamides/pharmacology , Titanium , Tumor Necrosis Factor-alpha/biosynthesis
6.
Mol Pharmacol ; 59(4): 860-6, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11259631

ABSTRACT

Transgenic mice (K5-PKC alpha) in which the keratin 5 promoter directs the expression of protein kinase C-alpha (PKC alpha) to epidermal keratinocytes display a 10-fold increase in PKC alpha protein in their epidermis and alterations in phorbol ester-induced cutaneous inflammation [J Cell Science 1999;112:3497-3506]. In the current study, we have used these K5-PKC alpha mice to examine the role of PKC alpha in keratinocyte phospholipid metabolism/eicosanoid production and cutaneous inflammation. Primary keratinocytes from wild-type and transgenic mice were prelabeled in culture with [(3)H]arachidonic acid (AA) and subsequently treated with TPA. Compared with wild-type keratinocytes, K5-PKC alpha keratinocytes displayed a 2-fold increase in AA release. TPA treatment resulted in the phosphorylation of cPLA(2). PKC inhibitors GF-109203X or H7, but not mitogen-activated protein/extracellular signal-regulated protein kinase (MEK) inhibitor PD 98059, could inhibit phosphorylation and AA release. Topical 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment of K5-PKC alpha mice resulted in a 5-fold increase in epidermal COX-2 induction and a 2- to 3-fold increase in prostaglandin (PG) E(2) levels above that observed in TPA-treated wild-type mice. PD 98059, GF-109203X, or H7 could block cyclooxygenase-2 (COX-2) induction by TPA. Because C/EBP beta, a basic leucine zipper transcription factor, can be activated via a PKC alpha/mitogen-activated protein kinase pathway and can influence COX-2 expression, we examined whether C/EBP beta is involved in TPA-induced epidermal COX-2 expression. TPA-induced COX-2 expression was similar in C/EBP beta nullizygous and wild-type mice. In summary, our results indicate that epidermal PKC alpha coordinately regulates cPLA(2) activity and COX-2 expression resulting in increased levels of AA and PGE(2). Furthermore, PKC alpha-induced AA release and cPLA(2) phosphorylation are independent of MEK, whereas PKC alpha-induced COX-2 expression and PGE(2) production are MEK-dependent and C/EBP beta-independent events.


Subject(s)
Cytosol/enzymology , Isoenzymes/biosynthesis , Isoenzymes/metabolism , Keratinocytes/metabolism , Phospholipases A/metabolism , Prostaglandin-Endoperoxide Synthases/biosynthesis , Protein Kinase C/metabolism , Animals , Arachidonic Acid/metabolism , CCAAT-Enhancer-Binding Protein-beta/metabolism , Cells, Cultured , Cyclooxygenase 2 , Dinoprostone/metabolism , Enzyme Inhibitors/pharmacology , Inflammation/metabolism , Isoenzymes/antagonists & inhibitors , Isoenzymes/genetics , Keratinocytes/cytology , Keratinocytes/drug effects , Keratins/genetics , Mice , Mice, Transgenic , Mitogen-Activated Protein Kinase Kinases/antagonists & inhibitors , Phospholipids/metabolism , Phosphorylation/drug effects , Promoter Regions, Genetic , Protein Isoforms/genetics , Protein Kinase C/antagonists & inhibitors , Protein Kinase C/genetics , Protein Kinase C-alpha , Signal Transduction/drug effects , Signal Transduction/physiology , Skin/drug effects , Skin/metabolism , Tetradecanoylphorbol Acetate/pharmacology
7.
Proc Natl Acad Sci U S A ; 98(3): 1059-64, 2001 Jan 30.
Article in English | MEDLINE | ID: mdl-11158594

ABSTRACT

The transition to pulmonary respiration following birth requires rapid alterations in the structure of the mammalian cardiovascular system. One dramatic change that occurs is the closure and remodeling of the ductus arteriosus (DA), an arterial connection in the fetus that directs blood flow away from the pulmonary circulation. A role for prostaglandins in regulating the closure of this vessel has been supported by pharmacological and genetic studies. The production of prostaglandins is dependent on two cyclooxygenases (COX-1 and COX-2), which are encoded by separate genes. We report here that the absence of either or both COX isoforms in mice does not result in premature closure of the DA in utero. However, 35% of COX-2(-/-) mice die with a patent DA within 48 h of birth. In contrast, the absence of only the COX-1 isoform does not affect closure of the DA. The mortality (35%) and patent DA incidence due to absence of COX-2 is, however, significantly increased (79%) when one copy of the gene encoding COX-1 is also inactivated. Furthermore, 100% of the mice deficient in both isoforms die with a patent DA within 12 h of birth, indicating that in COX-2-deficient mice, the contribution of COX-1 to DA closure is gene dosage-dependent. Together, these data establish roles for COX-1, and especially for COX-2, in the transition of the cardiopulmonary circulation at birth.


Subject(s)
Ductus Arteriosus, Patent/genetics , Isoenzymes/metabolism , Prostaglandin-Endoperoxide Synthases/metabolism , Animals , Animals, Newborn , Cyclooxygenase 1 , Cyclooxygenase 2 , Death , Ductus Arteriosus/pathology , Ductus Arteriosus, Patent/epidemiology , Female , Genomic Imprinting , Genotype , Isoenzymes/deficiency , Isoenzymes/genetics , Male , Membrane Proteins , Mice , Mice, Inbred C57BL , Mice, Inbred Strains , Mice, Knockout , Prostaglandin-Endoperoxide Synthases/deficiency , Prostaglandin-Endoperoxide Synthases/genetics , Time Factors
8.
Cancer Res ; 60(17): 4705-8, 2000 Sep 01.
Article in English | MEDLINE | ID: mdl-10987272

ABSTRACT

Two isoforms of cyclooxygenase (COX) are known, and to date most studies have implicated COX-2, rather than COX-1, as the isoform involved in colon carcinogenesis. In the present study, we show that homologous disruption of either Ptgs-1 or Ptgs-2 (genes coding for COX-1 or COX-2, respectively) reduced polyp formation in Min/+ mice by approximately 80%. Only COX-1 protein was immunohistochemically detected in normal intestinal tissue, whereas both COX-1 and variable levels of COX-2 protein were detected in polyps. Prostaglandin E2 was increased in polyps compared with normal tissue, and both COX-1 and COX-2 contributed to the PGE2 produced. The results indicate that COX-1, as well as COX-2, plays a key role in intestinal tumorigenesis and that COX-1 may also be a chemotherapeutic target for nonsteroidal anti-inflammatory drugs.


Subject(s)
Intestinal Neoplasms/enzymology , Intestinal Neoplasms/prevention & control , Intestinal Polyps/enzymology , Intestinal Polyps/prevention & control , Isoenzymes/genetics , Prostaglandin-Endoperoxide Synthases/genetics , Animals , Crosses, Genetic , Cyclooxygenase 1 , Cyclooxygenase 2 , Dinoprostone/biosynthesis , Female , Intestinal Neoplasms/genetics , Intestinal Polyps/genetics , Intestines/enzymology , Isoenzymes/deficiency , Male , Membrane Proteins , Mice , Mice, Inbred C57BL , Mice, Knockout , Prostaglandin-Endoperoxide Synthases/deficiency , Reference Values
9.
J Clin Invest ; 105(4): 469-78, 2000 Feb.
Article in English | MEDLINE | ID: mdl-10683376

ABSTRACT

To investigate roles in intestinal inflammation for the 2 cyclooxygenase (COX) isoforms, we determined susceptibility to spontaneous and induced acute colitis in mice lacking either the COX-1 or COX-2 isoform. We treated wild-type, COX-1(-/-), COX-2(-/-), and heterozygous mice with dextran sodium sulfate (DSS) to provoke acute colonic inflammation, and we quantified tissue damage, prostaglandin (PG) E(2), and interleukin-1beta. No spontaneous gastrointestinal inflammation was detected in mice homozygous for either mutation, despite almost undetectable basal intestinal PGE(2) production in COX-1(-/-) mice. Both COX-1(-/-) and COX-2(-/-) mice showed increased susceptibility to a low-dose of DSS that caused mild colonic epithelial injury in wild-type mice. COX-2(-/-) mice were more susceptible than COX-1(-/-) mice, and selective pharmacologic blockade of COX-2 potentiated injury in COX-1(-/-) mice. At a high dose, DSS treatment was fatal to 50% of the animals in each mutant group, but all wild-type mice survived. DSS treatment increased PGE(2) intestinal secretion in all groups except COX-2(-/-) mice. These results demonstrate that COX-1 and COX-2 share a crucial role in the defense of the intestinal mucosa (with inducible COX-2 being perhaps more active during inflammation) and that neither isoform is essential in maintaining mucosal homeostasis in the absence of injurious stimuli.


Subject(s)
Colitis/chemically induced , Intestinal Mucosa/pathology , Isoenzymes/deficiency , Prostaglandin-Endoperoxide Synthases/deficiency , Animals , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Colitis/mortality , Cyclooxygenase 1 , Cyclooxygenase 2 , Cyclooxygenase 2 Inhibitors , Cyclooxygenase Inhibitors/pharmacology , Dextrans/pharmacology , Dinoprostone/metabolism , Interleukin-1/metabolism , Isoenzymes/antagonists & inhibitors , Isoenzymes/genetics , Isoenzymes/pharmacology , Membrane Proteins , Mice , Mice, Mutant Strains , Nitrobenzenes/pharmacology , Prostaglandin-Endoperoxide Synthases/genetics , Prostaglandin-Endoperoxide Synthases/pharmacology , Sulfates/pharmacology , Sulfonamides/pharmacology
10.
Kidney Int ; 57(2): 414-22, 2000 Feb.
Article in English | MEDLINE | ID: mdl-10652018

ABSTRACT

BACKGROUND: Antenatal exposure to nonsteroidal anti-inflammatory drugs (NSAIDs) has been associated with renal dysgenesis in humans. METHODS: These studies characterized cyclooxygenase-2 (COX-2) versus COX-1-selective inhibition on nephrogenesis in the rodent using histomorphometry, immunohistology, and in situ hybridization. RESULTS: Administration of a COX-2-selective inhibitor (SC58236), started during pregnancy until weaning, significantly impaired development of the renal cortex and reduced glomerular diameter in both mice and rats. An identical phenotype was demonstrated in COX-2 -/- mice. In contrast to its effects on the developing kidney, a COX-2 inhibitor had no effect on glomerular volume in adult mice. This effect was specific for COX-2 because maternal administration of a COX-1-selective inhibitor (SC58560) did not affect renal development despite significantly inhibiting gastric mucosal prostaglandin E2 (PGE2) synthesis in pups. The expression of COX-2 immunoreactivity peaked in the first postnatal week and was localized to S-shaped bodies and the macula densa in the cortex. Treatment with a COX-2 inhibitor during this period (from postnatal day 0 to day 21) severely reduced glomerular diameter, whereas treatment limited to pregnancy did not affect glomerular size. CONCLUSION: These data demonstrate an important role for COX-2 activity in nephrogenesis in the rodent, and define a specific time period of susceptibility to these effects.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Cyclooxygenase Inhibitors/pharmacology , Isoenzymes/pharmacology , Kidney Cortex/embryology , Kidney Glomerulus/embryology , Organic Chemicals , Prostaglandin-Endoperoxide Synthases/pharmacology , Pyrazoles , Sulfonamides , Animals , Cyclooxygenase 1 , Cyclooxygenase 2 , Cyclooxygenase 2 Inhibitors , Dinoprostone/biosynthesis , Female , Gastric Mucosa/drug effects , Gastric Mucosa/enzymology , Gene Expression Regulation, Developmental/drug effects , Gene Expression Regulation, Enzymologic/drug effects , In Situ Hybridization , Isoenzymes/genetics , Isoenzymes/metabolism , Kidney Cortex/enzymology , Kidney Glomerulus/enzymology , Membrane Proteins , Mice , Mice, Inbred C57BL , Pregnancy , Prostaglandin-Endoperoxide Synthases/genetics , Prostaglandin-Endoperoxide Synthases/metabolism , RNA, Messenger/analysis , Rats , Rats, Sprague-Dawley
11.
Exp Cell Res ; 254(2): 232-40, 2000 Feb 01.
Article in English | MEDLINE | ID: mdl-10640421

ABSTRACT

Two isoforms of cyclooxygenase (COX-1 or COX-2) have been identified in the prostanoid biosynthetic pathway. The constitutive form, COX-1, is thought to maintain cellular homeostasis and the inducible form, COX-2, is recognized as a primary response gene thought to be involved in modulating cell proliferation and differentiation. To further characterize the role of the cyclooxygenases in cell proliferation, differentiation, and tumorigenicity we developed embryonic stem (ES) cell lines which contain homozygous disruptions in either the COX-1 or the COX-2 gene. These lines were then examined in terms of their viability, proliferation, and in vitro differentiation potential. Our results demonstrate that the wild-type ES cells do not express either COX-1 or COX-2 until the cells undergo differentiation. And the lack of either cyclooxygenase has no apparent effect on ES cell proliferation in vitro. However, the absence of a functional COX-2 gene leads to a dramatic reduction in the formation and growth of teratocarcinomas that appear when ES cells are injected into syngeneic mice. Histological microscopy shows that the few very small tumors that were generated from ES cells lacking COX-2 appear more differentiated than tumors emerging from COX-1 -/- or wild-type cells by exhibiting greater keratinization in the areas of squamous epithelium and the ossification of bone-forming cartilage. We conclude that the presence of a functional COX-2 enzyme is necessary for the efficient growth of these teratocarcinomas in animals.


Subject(s)
Isoenzymes/metabolism , Prostaglandin-Endoperoxide Synthases/metabolism , Teratocarcinoma/pathology , Teratocarcinoma/prevention & control , Animals , Cell Differentiation , Cell Division/genetics , Cell Transformation, Neoplastic , Cyclooxygenase 1 , Cyclooxygenase 2 , Genotype , Isoenzymes/deficiency , Isoenzymes/genetics , Membrane Proteins , Mice , Mice, Knockout , Prostaglandin-Endoperoxide Synthases/deficiency , Prostaglandin-Endoperoxide Synthases/genetics , Stem Cell Transplantation , Stem Cells/cytology , Stem Cells/physiology , Teratocarcinoma/genetics , Transplantation, Isogeneic
12.
Biochem Biophys Res Commun ; 265(1): 205-10, 1999 Nov.
Article in English | MEDLINE | ID: mdl-10548515

ABSTRACT

Activation of mast cells by aggregation of their high-affinity IgE receptors stimulates prostaglandin (PG) D(2) synthesis and secretion. An immediate phase of PGD(2) synthesis, complete within 30 min, is followed by a delayed, second phase of PGD(2) production that reaches a maximum 4 to 8 h after activation. Activation of mast cells from COX-2 (-/-) mice stimulates the release of PGD(2) during the first 30 min, whereas activation of mast cells from COX-1 (-/-) mice does not generate any PGD(2) in the first 2 h. On the other hand, COX-2 (-/-) cells do not participate in delayed phase of PGD(2) synthesis, while COX-1 (-/-) cells secrete low levels of PGD(2) between 2 and 4 h after activation. These data demonstrate that (i) the first phase of PG synthesis is COX-1 dependent and (ii) the second, delayed phase of PG synthesis is dependent on activation-induced synthesis and activity of COX-2.


Subject(s)
Isoenzymes/genetics , Isoenzymes/metabolism , Mast Cells/physiology , Prostaglandin-Endoperoxide Synthases/genetics , Prostaglandin-Endoperoxide Synthases/metabolism , Prostaglandins/biosynthesis , Animals , Bone Marrow Cells/cytology , Cell Line , Cyclooxygenase 1 , Cyclooxygenase 2 , Isoenzymes/deficiency , Kinetics , Mast Cells/enzymology , Membrane Proteins , Mice , Mice, Knockout , Polymerase Chain Reaction , Prostaglandin D2/biosynthesis , Prostaglandin-Endoperoxide Synthases/deficiency , Time Factors
13.
J Clin Invest ; 104(6): 721-32, 1999 Sep.
Article in English | MEDLINE | ID: mdl-10491407

ABSTRACT

To investigate the function of prostaglandin H synthase-1 and synthase-2 (PGHS-1 and PGHS-2) in the normal lung and in allergic lung responses, we examined allergen-induced pulmonary inflammation and airway hyperresponsiveness in wild-type mice and in PGHS-1(-/-) and PGHS-2(-/-) mice. Among nonimmunized saline-exposed groups, we found no significant differences in lung function or histopathology, although PGE(2) was dramatically reduced in bronchoalveolar lavage (BAL) fluid from PGHS-1(-/-) mice, relative to wild-type or PGHS-2(-/-) mice. After ovalbumin sensitization and challenge, lung inflammatory indices (BAL cells, proteins, IgE, lung histopathology) were significantly greater in PGHS-1(-/-) mice compared with PGHS-2(-/-) mice, and both were far greater than in wild-type mice, as illustrated by the ratio of eosinophils in BAL fluid (8:5:1, respectively). Both allergic PGHS-1(-/-) and PGHS-2(-/-) mice exhibited decreased baseline respiratory system compliance, whereas only allergic PGHS-1(-/-) mice showed increased baseline resistance and responsiveness to methacholine. Ovalbumin exposure caused a modest increase in lung PGHS-2 protein and a corresponding increase in BAL fluid PGE(2) in wild-type mice. We conclude that (a) PGHS-1 is the predominant enzyme that biosynthesizes PGE(2) in the normal mouse lung; (b) PGHS-1 and PGHS-2 products limit allergic lung inflammation and IgE secretion and promote normal lung function; and (c) airway inflammation can be dissociated from the development of airway hyperresponsiveness in PGHS-2(-/-) mice.


Subject(s)
Hypersensitivity/etiology , Isoenzymes/physiology , Lung/immunology , Prostaglandin-Endoperoxide Synthases/physiology , Allergens/immunology , Animals , Bronchoalveolar Lavage Fluid/cytology , Dinoprostone/biosynthesis , Female , Immunoglobulin E/analysis , Isoenzymes/deficiency , Leukotriene B4/biosynthesis , Lung/pathology , Lung Compliance , Lysosomes/enzymology , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Prostaglandin-Endoperoxide Synthases/deficiency
14.
Biochem Pharmacol ; 58(8): 1237-46, 1999 Oct 15.
Article in English | MEDLINE | ID: mdl-10487525

ABSTRACT

The development of cyclooxygenase (COX) deficient mice has allowed investigation into the individual physiological roles of the COX-1 and COX-2 isoforms. In the following article, the phenotypes of the two Ptgs (genes coding for COX-1 and COX-2) knockouts are summarized, and recent studies to investigate the effects of COX deficiency on cancer susceptibility, inflammatory response, gastric ulceration, and female reproductive processes are discussed. Also, the development and potential uses of mice deficient in both COX isoforms and mice containing only a single copy of one isoform are discussed. Additionally, when the data permit, the effects of genetic ablation of COX activity are compared with those of pharmacological inhibition of COX activity by nonsteroidal anti-inflammatory drugs. The data suggest that prostaglandins derived via the individual COX isoforms have separate as well as common functions. However, for the maintenance of normal physiology, it appears that deficiency of COX-2 has more profound effects than deficiency of COX-1.


Subject(s)
Isoenzymes/metabolism , Prostaglandin-Endoperoxide Synthases/metabolism , Animals , Cell Transformation, Neoplastic , Cyclooxygenase 1 , Cyclooxygenase 2 , Female , Humans , Inflammation/enzymology , Isoenzymes/deficiency , Isoenzymes/genetics , Membrane Proteins , Mice , Mice, Knockout , Phenotype , Prostaglandin-Endoperoxide Synthases/deficiency , Prostaglandin-Endoperoxide Synthases/genetics , Reproduction/physiology , Stomach Ulcer/enzymology
15.
J Exp Med ; 190(4): 451-59, 1999 Aug 16.
Article in English | MEDLINE | ID: mdl-10449516

ABSTRACT

In this study, we use primary embryonic fibroblasts derived from cyclooxygenase-deficient transgenic embryos to further investigate the role of the two cyclooxygenases, cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2), in the process of neoplastic transformation. Cells with either, neither, or both of the cyclooxygenases were transformed by Ha-ras and/or SV40. Our results show that when a cyclooxygenase enzyme is present, the transformed cells have marked increases in COX-2 and/or COX-1 expression. Nevertheless, each type of cell, deficient in either or both cyclooxygenases, can be readily transformed at almost equal efficiency. Different nonsteroidal antiinflammatory drugs (NSAIDs) were used to examine their possible antineoplastic effects on the transformed cells, which have various levels of expression of COX-1 or COX-2. Our results show that NSAIDs suppress the colony formation in soft agar in a dosage-dependent manner in the absence of the cyclooxygenase(s). Thymidine incorporation and apoptosis analyses further demonstrate that the NSAIDs are effective in the cyclooxygenase-null cells. Our findings with cyclooxygenase knockout cells confirm recent reports that some of the antiproliferative and antineoplastic effects of NSAIDs are independent of the inhibition of either COX-1 or COX-2. They also show that transformation is independent of the status of cyclooxygenase expression, suggesting that the involvement of the cyclooxygenases in tumorigenesis may occur at later steps.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Antineoplastic Agents/pharmacology , Cell Transformation, Neoplastic/drug effects , Isoenzymes/deficiency , Prostaglandin-Endoperoxide Synthases/deficiency , Animals , Arachidonic Acid/metabolism , Cell Line , Cell Transformation, Viral , Cyclooxygenase 1 , Cyclooxygenase 2 , Dinoprostone/biosynthesis , Embryo, Mammalian/cytology , Fibroblasts/cytology , Ibuprofen/pharmacology , Indomethacin/pharmacology , Isoenzymes/genetics , Membrane Proteins , Mice , Mice, Knockout , Nitrobenzenes/pharmacology , Oncogene Proteins, Viral , Piroxicam/pharmacology , Prostaglandin-Endoperoxide Synthases/genetics , Proto-Oncogene Proteins p21(ras)/genetics , RNA, Messenger/isolation & purification , Simian virus 40 , Sulfonamides/pharmacology , Sulindac/pharmacology
16.
Endocrinology ; 140(6): 2685-95, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10342859

ABSTRACT

Mice carrying a null mutation for either of the two cyclooxygenase (COX) isoenzymes, necessary for prostanoid production, exhibit several isotype-specific reproductive abnormalities. Mice deficient in COX-1 are fertile but have decreased pup viability, whereas mice deficient in COX-2 fail to ovulate and have abnormal implantation and decidualization responses. The present study identifies the specific contribution of each COX isoenzyme in hypothalamic, pituitary, and ovarian function and establishes the pathology and rescue of the anovulatory syndrome in the COX-2-deficient mouse. In both COX-1- and COX-2-deficient mice, pituitary gonadotropins were selectively increased, whereas hypothalamic LHRH and serum gonadotropin levels were similar to those in wild-type animals (+/+). No significant differences in serum estrogen or progesterone were noted among the three genotypes. Exogenous gonadotropin stimulation with PMSG and hCG produced a comparable 4-fold increase in ovarian PGE2 levels in wild-type and COX-1(-/-) mice. COX-2(-/-) mice had no increase in PGE2 over PMSG-stimulated levels. Wild-type and COX-1(-/-) mice ovulated in response to PMSG/hCG; very few COX-2(-/-) animals responded to this regimen. The defect in ovulation in COX-2 mutants was attributed to both an abnormal cumulus oophorum expansion and subsequent stigmata formation. Gonadotropin stimulation and concurrent treatment with PGE2 or interleukin-1beta resulted in ovulation of COX-2(-/-) mice comparable to that in COX-2(+/+), whereas treatment with PGF2alpha was less effective. Collectively, these data demonstrate that COX-2, but not COX-1, is required for the gonadotropin induction of ovarian PG levels; that COX-2-related prostanoids are required for stabilization of the cumulus oophorum during ovulation; and that ovulation can be restored in the COX-2(-/-) animals by simultaneous treatment with gonadotropins and PGE2 or interleukin-1beta.


Subject(s)
Anovulation/drug therapy , Dinoprostone/pharmacology , Interleukin-1/pharmacology , Isoenzymes/physiology , Prostaglandin-Endoperoxide Synthases/physiology , Animals , Anovulation/etiology , Chorionic Gonadotropin/pharmacology , Cyclooxygenase 1 , Cyclooxygenase 2 , Dinoprostone/analysis , Estrus , Female , Fertility , Follicle Stimulating Hormone/analysis , Isoenzymes/deficiency , Membrane Proteins , Mice , Ovulation/drug effects , Prostaglandin-Endoperoxide Synthases/deficiency
17.
J Clin Invest ; 103(10): 1469-77, 1999 May 15.
Article in English | MEDLINE | ID: mdl-10330429

ABSTRACT

Prostaglandin G and H synthases, or cyclooxygenases (COXs), catalyze the formation of prostaglandins (PGs). Whereas COX-1 is diffusely expressed in lymphoid cells in embryonic day 15.5 thymus, COX-2 expression is sparse, apparently limited to stromal cells. By contrast, COX-2 is predominant in a subset of medullary stromal cells in three- to five-week-old mice. The isozymes also differ in their contributions to lymphocyte development. Thus, experiments with selective COX-1 inhibitors in thymic lobes from normal and recombinase-activating gene-1 knockout mice support a role for this isoform in the transition from CD4(-)CD8(-) double-negative (DN) to CD4(+)CD8(+) double-positive (DP). Concordant data were obtained in COX-1 knockouts. Pharmacological inhibition and genetic deletion of COX-2, by contrast, support its role during early thymocyte proliferation and differentiation and, later, during maturation of the CD4 helper T-cell lineage. PGE2, but not other PGs, can rescue the effects of inhibition of either isoform, although it acts through distinct EP receptor subtypes. COX-dependent PG generation may represent a mechanism of thymic stromal support for T-cell development.


Subject(s)
Isoenzymes/physiology , Prostaglandin-Endoperoxide Synthases/physiology , T-Lymphocytes/cytology , T-Lymphocytes/enzymology , Animals , Base Sequence , CD4 Antigens/metabolism , CD8 Antigens/metabolism , Cell Differentiation/drug effects , Cyclooxygenase 1 , Cyclooxygenase 2 , Cyclooxygenase 2 Inhibitors , Cyclooxygenase Inhibitors/pharmacology , DNA Primers/genetics , Dinoprostone/pharmacology , Gene Expression , Genes, RAG-1 , Isoenzymes/genetics , Membrane Proteins , Mice , Mice, Inbred C57BL , Mice, Knockout , Models, Biological , Prostaglandin-Endoperoxide Synthases/genetics , T-Lymphocytes/immunology
18.
Ann N Y Acad Sci ; 889: 52-61, 1999.
Article in English | MEDLINE | ID: mdl-10668482

ABSTRACT

Cyclooxygenase (COX)-1- and COX-2-deficient mice have unique physiological differences that have allowed investigation into the individual biological roles of the COX isoforms. In the following, the phenotypes of the two COX knockout mice are summarized, and recent studies to investigate the effects of COX deficiency on inflammatory responses and cancer susceptibility are discussed. The data suggest that both isoforms have important roles in the maintenance of physiological homeostasis and that such designations as house-keeping and/or response gene may not be entirely accurate. Furthermore, data from COX-deficient mice indicate that both isoforms can contribute to the inflammatory response and that both isoforms have significant roles in carcinogenesis.


Subject(s)
Disease Models, Animal , Inflammation/genetics , Isoenzymes/genetics , Mice, Knockout , Neoplasms, Experimental/genetics , Prostaglandin-Endoperoxide Synthases/genetics , Animals , Cyclooxygenase 1 , Cyclooxygenase 2 , Genetic Predisposition to Disease , Inflammation/etiology , Membrane Proteins , Mice , Neoplasms, Experimental/etiology
19.
Cell ; 91(2): 197-208, 1997 Oct 17.
Article in English | MEDLINE | ID: mdl-9346237

ABSTRACT

Cyclooxygenase (COX) is the rate-limiting enzyme in the synthesis of prostaglandins (PGs) and exists in two isoforms, COX-1 and COX-2. In spite of long-standing speculation, definitive roles of PGs in various events of early pregnancy remain elusive. We demonstrate herein that the targeted disruption of COX-2, but not COX-1, in mice produces multiple failures in female reproductive processes that include ovulation, fertilization, implantation, and decidualization. Using multiple approaches, we conclude that these defects are the direct result of target organ-specific COX-2 deficiency but are not the result of deficiency of pituitary gonadotropins or ovarian steroid hormones, or reduced responsiveness of the target organs to their respective hormones.


Subject(s)
Embryo Implantation/genetics , Fertilization/genetics , Isoenzymes/genetics , Mice, Knockout/physiology , Ovulation/genetics , Prostaglandin-Endoperoxide Synthases/genetics , Animals , Blastocyst/physiology , Cholera Toxin/pharmacology , Cyclooxygenase 1 , Cyclooxygenase 2 , Decidua/drug effects , Dose-Response Relationship, Drug , Embryo Implantation/drug effects , Enzyme Inhibitors/pharmacology , Epoprostenol/pharmacology , Female , Fertilization/drug effects , Gene Expression Regulation/physiology , Isoenzymes/deficiency , Membrane Proteins , Mice , Mice, Inbred C57BL , Ovulation/drug effects , Peroxidases/deficiency , Peroxidases/genetics , Platelet Aggregation Inhibitors/pharmacology , Pregnancy , Prostaglandin-Endoperoxide Synthases/deficiency , Pseudopregnancy , RNA, Messenger/analysis , Receptors, Prostaglandin E/genetics , Steroids/physiology , Uterus/physiology
20.
J Pharmacol Exp Ther ; 280(2): 606-13, 1997 Feb.
Article in English | MEDLINE | ID: mdl-9023270

ABSTRACT

Mouse embryonic fibroblasts (10T1/2) and Chinese hamster ovary (AS52) cell lines that stably express murine prostaglandin G/H synthase (PGHS)-1 or -2 were used to compare the effects of exogenous and endogenous arachidonic acid (AA) on isozyme-selective inhibition by acetylsalicylic acid, indomethacin, and N-[2-cyclohexyloxyl-4-nitrophenyl] methanesulfonamide (NS-398). The rationale for developing in vitro systems that identify PGHS-2-selective inhibitors is the belief that inhibition of this isoform accounts for the therapeutic benefits of nonsteroidal anti-inflammatory drugs (NSAIDs). Conversely, inhibition of PGHS-1 is believed to cause the toxic effects of NSAIDs, such as gastric and renal damage. When exogenous AA was used, acetylsalicylic acid was a 5- to 10-fold more potent inhibitor of PGHS-1, whereas indomethacin was a 4- to 5-fold more potent inhibitor of PGHS-2. Within the dose range tested (1 x 10(-6) microM to 100 microM), NS-398 was highly selective for PGHS-2. When calcium ionophore A23187 was used to mobilize endogenous AA, acetylsalicylic acid and indomethacin equipotently inhibited both PGHS-1 and PGHS-2 isozymes. NS-398 remained highly selective for PGHS-2 in 10T1/2 and AS52 cells but also effectively (100%) inhibited PGHS-1 in AS52 cells. Pharmacological data derived using endogenous AA correlated better with the anti-inflammatory efficacy of these NSAIDs in laboratory animals and with the therapeutic/toxic activities of these NSAIDs in rheumatoid arthritic patients. Therefore, screening for PGHS-selective NSAIDs may best be conducted in intact cells that express high levels of each isozyme using endogenous sources of AA.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Arachidonic Acid/metabolism , Cyclooxygenase Inhibitors/pharmacology , Isoenzymes/metabolism , Prostaglandin-Endoperoxide Synthases/metabolism , Animals , Aspirin/pharmacology , CHO Cells , Calcimycin/pharmacology , Cell Line , Cell Survival/drug effects , Cricetinae , Cyclooxygenase 1 , Cyclooxygenase 2 , Cyclooxygenase 2 Inhibitors , Indomethacin/pharmacology , Kinetics , Membrane Proteins , Mice , Nitrobenzenes/pharmacology , Sulfonamides/pharmacology
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