ABSTRACT
BACKGROUND: Statherin is a small phosphoprotein chiefly studied for its protective roles towards teeth and oral tissues. Although generally considered as exclusively secreted by salivary glands, circumstantial evidences suggested that other tissues also produce it. This article first demonstrates statherin immunoreactivity in human prostate and seminal vesicles. METHODS: Surgical samples of prostate and seminal vesicles were fixed in a mixture of paraformaldehyde and glutaraldehyde, and embedded in Epon resin without previous osmication. Ultrathin sections were treated for the intracellular localization of statherin by means of an immunogold staining method. RESULTS: Reactive statherin was revealed in secreting cells of both seminal vesicle and prostate epithelia: labeling was found in secretory granules of seminal vesicle cells and in cytoplasmic vesicles of prostatic cells. CONCLUSIONS: The different staining patterns suggested that the two glands secrete statherin through different pathways. Prostate 71:671-674, 2011. © 2010 Wiley-Liss, Inc.
Subject(s)
Prostate/metabolism , Salivary Proteins and Peptides/metabolism , Seminal Vesicles/metabolism , Aged , Epithelium/metabolism , Epithelium/ultrastructure , Humans , Immunohistochemistry , Male , Microscopy, Electron, Transmission , Middle Aged , Prostate/ultrastructure , Salivary Proteins and Peptides/analysis , Seminal Vesicles/ultrastructureABSTRACT
A method is described for embedment of multiple confluent sheets of flat tissue culture cells that permits sectioning for thin or semithin sections in precise planes. The technique is especially useful for obtaining en face sections.
