The effect of essential oil of Ammoides pusilla (brot.) breistr on the growth and the production of solanapyrone a by Ascochyta rabiei.

Biological control such as the use of plant extracts has emerged as promising option to the phenomena of fungi resistance to chemical. Several constituent of essential oil have been studied for their biological activity including antibacterial and antifungal activity. In this study the effect of Ammoides pusilla essential oil with different concentrations was test against the growth of Ascochyta rabiei and the production of solanapyrone A by the fungus. After 14 days the mycelium was collected and the dry weight measured. A. rabiei did not grow at a final concentration of 6 and 3 mg/ml, at 1.5 mg/ml and 0.625ml there was little growth of the fungus with a dry weight of 55 mg and 99 mg respectively compared to the control with 519 mg dry weight, but there was no solanapyrone A produced. However a new compound appeared at the HPLC at 10 min. 30 sec. compared with the solanapyrone A which elutes at nearly 14 minutes.

Study of the effect of Ammoides pusilla (Brot.) Breist, essential oil against Pseudomonas sp.

The economic losses caused by plant pathogens, the development of genetic resistance to applied pesticides by major pathogenic bacteria and public concerns about the use of synthetic chemicals on food and environment, increased interest in the search of alternative safe methods to control diseases spreading. Biological control with plant extracts has emerged as a promising option. The present study was carried out to determine the effect of Ammoides pusilla essential oil on the growth of two pathogenic bacteria, Pseudomonas syringae pv. syringae and Pseudomonas syringae pv. mosprunorum Three essential oil treatments, 1/2 v/v, 1/5 v/v and 1/10 v/v concentrations, were tested onto these bacteria, using the agar diffusion test. The results showed that 1/2 v/v concentration treatment completely inhibited Pseudomonas syringae pv. mosprunorum growth and produced a 35 mm diameter inhibition zone for Pseudomonas syringae pu. syringae. 1/5 v/v essential oil concentration produced inhibition zones of 19 and 17 mm diameter for Pseudomonas syringae pv. syringae and Pseudormonas syringae pv. mosprunorum respetively, while 1/10 v/v essential oil concentration gave inhibition plates of 11 and 12 mm diameter for both stains in the order cited. In in vitro culture essential oil gave promising results suggesting that future studies should be carried out and focus on determining appropriate formulation of this bio-molecule to be use as a bio-control agent.

Laboratory evaluation of Melia azedarach L. and Eucalyptus globulus Labill. Extracts in order to control Ocneridia volxemi Bolivar (Orthoptera, Pamphaginae) hoppers.

This study was carried out to evaluate the effect of Melia azedarach L. (Meliaceae) dry fruit extracts (oil and water) and Eucalyptus globulus Labill. (Myrtaceae) dry leaves extracts (water) on Oneridia volxemi hoppers (fifth-instar) feeding on cereals leaves (Triticum durum) under laboratory conditions. Larva feeding reduced and mortality was significatly (p < 0.05) on cereals leaves sprayed with three concentrations 0, 5, 1 and 2% of oil extracted from Melia azederach. In addition, O. volxemi feeding and mortality was significantly (p < 0.05) on cereals leaves sprayed with two concentrations 50 g/l and 80 g/l of water extracts from Melia azedarach dry fruits soaked for 24 hours. the water extract solution of 80 g/l significantly reduced feeding than the other concentrations 50 and 25 g/l. This study showed also the water extract solution from Eucalyptus globulus dry leaves reduced also feeding and induced the mortality of O. volxemi but not better than Melia azedarach dry fruit extracts water at the same concentration (80 g/l). The aim of this study is in an integrated management program for control of O.

[Operative risk factors and polyvalvular cardiac surgery]. / Facteurs de risque opératoire en chirurgie cardiaque polyvalvulaire.

The aim of this study was to identify risk factors for morbidity and mortality in cardiac valvular surgery. Two hundred and fifty-four patients underwent polyvalvular surgery between May 1996 and December 1998. The mean age was 36.8 years (range 4-66 years) and the group comprised 148 women. Two subgroups were defined: the first (184 patients), characterised by associated mitral and tricuspid valve disease: the second (70 patients), characterised by associated mitral and aortic valve disease with a tricuspid lesion in about half the cases. The risk factors of morbidity and mortality analysed were: Clinical: previous history of cardiac failure, NYHA classification, atrial fibrillation and cardiothoracic index. Doppler echocardiographic: left ventricular end systolic dimension > 50 mm, left ventricular and diastolic dimension > 70 mm, fractional shortening < 28%, ejection fraction < 40%: systolic pulmonary artery pressure > 30 mmHg. Haemodynamic: capillary wedge pressure > 20 mmHg, systolic pulmonary artery pressure > 30 mmHg. The authors identified three statistically significant risk factors for operative morbidity and mortality in polyvalvular cardiac surgery: Previous history of one or more episodes of cardiac failure NYHA functional Classes III or IV fractional shortening < 28%. The morbidity and mortality were lower when patients were operated before these poor prognostic factors were observed.