ABSTRACT
The antagonistic activity of strains Bacillus amyloliquefaciens YKM B-7243 and YKM B-7404 against bacterial and fungal agents of winter wheat diseases was demonstrated. Low antagonistic level against bacterial agents of plant diseases was shown. Antifungal activity of strains against agents of winter wheat was on the low level. High biological efficiency was shown under binary compositions of the studied strains. It was concluded that the enhancing of fungicide and growth-stimulation effects in binary compositions were found.
Subject(s)
Antibiosis , Bacillus/physiology , Biological Control Agents , Plant Diseases , Triticum/microbiology , Bacillus/chemistry , Bacillus/growth & development , Plant Diseases/microbiology , Plant Diseases/prevention & control , Triticum/growth & developmentABSTRACT
Synthesis of extracellular phytohormones by bacteria strains of the genus Bacillus isolated from different ecological sources (soil and plant) has been researched. It has been shown that phytohormone's synthesis ability of the endophytic and free-living Bacillus strains is significantly different as regards the level and spectrum of synthesized compounds. It was concluded that such differences in synthesis of extracellular phytohormones by bacilli are associated with different type of interactions between the studied microorganisms and host plant.
Subject(s)
Antibiosis , Bacillus subtilis/metabolism , Endophytes/metabolism , Plant Growth Regulators/biosynthesis , Plants/microbiology , Soil Microbiology , Bacillus subtilis/growth & development , Bacillus subtilis/isolation & purification , Biomass , Endophytes/growth & development , Endophytes/isolation & purificationABSTRACT
This work was aimed at the study of biological and genetic peculiarities of the strains Bacillus subtilis 2/10 and 63z which were selected for protection of strawberries from Botrytis fructigena and for apples under storage. The obtained strains were shown to possess a broad spectrum of antagonistic activity against phytopathogenic fungi and bacteria and this activity was manifested at low temperatures specific for the agriculture production storage. These cultures were not toxigenic or pathogenic in respect of plants and hot-blooded. By RAPD-typing and sequencing of the cloned parts of DNA the genetic specificity of the selected cultures was shown compared with other Bacillus subtilis strains which had not been so active. A set of genetic primes was selected to allow one to reliably identify the given strains during production of biopreparations and to control their use in the field conditions.
Subject(s)
Bacillus subtilis/physiology , Food Microbiology , Fragaria/microbiology , Fruit/microbiology , Bacillus subtilis/genetics , Botrytis/physiology , Random Amplified Polymorphic DNA TechniqueABSTRACT
AIMS: To study Bacillus contamination of wheat flour and ropy bread, to analyse genetic diversity of isolated strains and to evaluate the ability of these strains to produce ropy bread. METHODS AND RESULTS: Classical and molecular methods [16S rDNA sequencing and random amplified polymorphic DNA (RAPD)-PCR] were used to identify and type-isolated strains. The predominant species isolated were Bacillus subtilis and B. licheniformis. RAPD analysis demonstrated that the same sample may harbor different strains. Ten of 15 strains of B. subtilis and four of six strains of B. licheniformis were able to cause rope spoilage of the laboratory-baked bread. CONCLUSION: RAPD typing can be useful in the tracking of Bacillus strains during bakery processing and in the understanding of the role of different Bacillus strains in the rope spoilage of bread. SIGNIFICANCE AND IMPACT OF THE STUDY: The results indicate the variability of Bacillus strains isolated from flour and responsible for rope spoilage of bread.
