Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
2.
BMC Bioinformatics ; 9: 218, 2008 Apr 28.
Article in English | MEDLINE | ID: mdl-18442400

ABSTRACT

BACKGROUND: It is useful to develop a tool that would effectively describe protein mutation matrices specifically geared towards the identification of mutations that produce either wanted or unwanted effects, such as an increase or decrease in affinity, or a predisposition towards misfolding. Here, we describe a tool where such mutations are efficiently identified, categorized and visualized. To categorize the mutations, amino acids in a mutation matrix are arranged according to one of three sets of physicochemical characteristics, namely hydrophilicity, size and polarizability, and charge and polarity. The magnitude and frequencies of mutations for an alignment are subsequently described using color information and scaling factors. RESULTS: To illustrate the capabilities of our approach, the technique is used to visualize and to compare mutation patterns in evolving sequences with diametrically opposite characteristics. Results show the emergence of distinct patterns not immediately discernible from the raw matrices. CONCLUSION: Our technique enables effective categorization and visualization of mutations by using specifically-arranged mutation matrices. This tool has a number of possible applications in protein engineering, notably in simplifying the identification of mutations and/or mutation trends that are associated with specific engineered protein characteristics and behavior.


Subject(s)
Amino Acids/chemistry , Amino Acids/classification , Audiovisual Aids , Mutation , Proteins/genetics , User-Computer Interface , Amyloid/immunology , Animals , Antibodies/analysis , Antibodies/genetics , Computer Graphics , Databases, Protein , Electric Capacitance , Hemagglutinins/analysis , Hemagglutinins/genetics , Humans , Hydrophobic and Hydrophilic Interactions , Iodide Peroxidase/immunology , Mutagenesis , Protein Folding , Proteins/chemistry , Receptors, Odorant/analysis , Receptors, Odorant/genetics , Research Design , Sequence Alignment , Sequence Analysis, Protein , Static Electricity
SELECTION OF CITATIONS
SEARCH DETAIL
...