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2.
Blood ; 92(1): 291-9, 1998 Jul 01.
Article in English | MEDLINE | ID: mdl-9639529

ABSTRACT

The role of neutrophils during Epstein-Barr virus (EBV) infection is not known. Disruption of the initial and nonspecific immune response may favor the spread of EBV infection. We have previously shown that EBV interacts with human neutrophils and modulates protein expression. In this study we have investigated the ability of EBV to infect neutrophils. Electron microscopy studies showed penetration of virus and its subsequent localization to the nucleus. The presence of viral genomes in isolated nuclei from neutrophils was also shown by polymerase chain reaction (PCR). Expression of viral transcripts like EBNA-2 (Epstein-Barr nuclear antigen-2) and ZEBRA (BamHI Z EBV replication activator) was not detected by reverse transcriptase (RT)-PCR, suggesting that EBV does not seem to establish a latent or a lytic infection in neutrophils. However, at 20 hours post-EBV infection, 77% of cells were apoptotic as compared to 22% in uninfected cell cultures, as evaluated by flow cytometry. This EBV-induced apoptosis was prevented by the addition of granulocyte-macrophage colony-stimulating factor to the cell cultures. Apoptotic cell death seems to implicate the Fas/Fas ligand (L) pathway, as reflected by an increase of Fas/Fas L expression on neutrophils treated with EBV and an increase of soluble Fas L, which may function in an autocrine/paracrine pathway to mediate cell death. Lastly, EBV genome was detected from neutrophils of infectious mononucleosis (IM) patients in contrast to neutrophils obtained from healthy EBV-seropositive donors. Our findings on the interactions of EBV with neutrophils will then provide new insights on the immunosuppressive effects associated with EBV infection.


Subject(s)
Apoptosis , Herpesviridae Infections/pathology , Herpesvirus 4, Human , Neutrophils/pathology , Neutrophils/virology , Tumor Virus Infections/pathology , Apoptosis/physiology , Fas Ligand Protein , Humans , Membrane Glycoproteins/physiology , Neutrophils/physiology , fas Receptor/physiology
3.
J Immunol ; 160(5): 2442-8, 1998 Mar 01.
Article in English | MEDLINE | ID: mdl-9498788

ABSTRACT

We have recently demonstrated that EBV binds to human neutrophils and stimulates a wide range of activities, including homeotypic aggregation, total RNA synthesis, and expression of the chemokines IL-8 and macrophage inflammatory protein-1alpha (MIP-1alpha). Neutrophil function is also known to be modulated by priming with granulocyte-macrophage colony-stimulating factor (GM-CSF). We have therefore investigated the modulation of EBV-induced activation of human neutrophils by GM-CSF. Treatment of neutrophils with GM-CSF before EBV activation enhanced the production of both MIP-1alpha and IL-8. The IL-8 produced under these conditions was biologically active as determined in the calcium mobilization assay. GM-CSF was also found to increase the ability of EBV to prime neutrophils for increased leukotriene B4 (LTB4) synthesis. Prior treatment of GM-CSF with neutralizing Abs inhibited these effects. GM-CSF also increased the specific binding of FITC-EBV to the neutrophil surface, as evaluated by fluorocytometry. Local production of GM-CSF in tissues invaded by EBV could therefore serve to potentiate a host defense mechanism directed toward the destruction of the infectious virus via increased production of chemotactic factors. Since both IL-8 and MIP-1alpha are reported to be chemoattractants in vitro for T cells and T and B cells, respectively, the ability of EBV to induce their production by neutrophils may enhance its ability to infect B and T lymphocytes via increased recruitment to sites of infection.


Subject(s)
Adjuvants, Immunologic/pharmacology , Chemotactic Factors/biosynthesis , Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology , Herpesvirus 4, Human/immunology , Neutrophils/immunology , Neutrophils/metabolism , Calcium/metabolism , Chemokine CCL3 , Chemokine CCL4 , Herpesvirus 4, Human/physiology , Humans , Interleukin-8/biosynthesis , Interleukin-8/genetics , Interleukin-8/metabolism , Leukotriene B4/metabolism , Macrophage Inflammatory Proteins/biosynthesis , Macrophage Inflammatory Proteins/genetics , Neutrophil Activation/drug effects , Neutrophil Activation/immunology , Neutrophils/virology , RNA, Messenger/metabolism
4.
Virology ; 238(2): 344-52, 1997 Nov 24.
Article in English | MEDLINE | ID: mdl-9400607

ABSTRACT

Neutrophils play an important role in the control of viral infections by releasing a variety of potent agents. We previously demonstrated that Epstein-Barr virus (EBV) binds to human neutrophils and stimulates cytokine synthesis including interleukin-1 (IL-1) and IL-1 receptor antagonist (IL-1Ra). Since neutrophil functions are known to be modulated by the priming effect of granulocyte-macrophage colony-stimulating factor (GM-CSF), we therefore investigated the cellular source of GM-CSF synthesis following treatment of leukocytes with EBV and the effect of GM-CSF on the production of IL-1, IL-1Ra, and superoxide by EBV-treated neutrophils. In enriched-cell populations, only monocytes were found to produce GM-CSF in response to EBV, which was maximal after 12 h of incubation. The results obtained with UV-irradiated particles or EBV neutralized with monoclonal antibody 72A1 suggest that contact between the cell and the gp350 of the viral envelope is sufficient to induce the release of GM-CSF. On the other hand, GM-CSF differentially upregulated EBV-induced IL-1 and IL-1Ra production by neutrophils. Pretreatment of neutrophils with GM-CSF prior to EBV activation synergistically enhanced the production of IL-1 alpha and IL-1 beta, but only marginally affected IL-1Ra synthesis. In addition, GM-CSF was also found to synergistically enhance the superoxide production by neutrophils in response to EBV. Molecular analysis showed that GM-CSF did not alter the IL-1 beta and IL-1Ra mRNA synthesis induced by EBV, suggesting that GM-CSF could act at a posttranslational level. Local production of GM-CSF by monocytes in tissues invaded by EBV could serve to potentiate the host defense mechanisms directed toward the destruction of the infectious virus.


Subject(s)
Granulocyte-Macrophage Colony-Stimulating Factor/biosynthesis , Herpesvirus 4, Human/physiology , Interleukin-1/metabolism , Neutrophils/metabolism , Neutrophils/virology , Receptors, Interleukin-1/biosynthesis , Sialoglycoproteins/biosynthesis , Animals , Blotting, Northern , Callithrix , Cell Line, Transformed , Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology , Humans , Interleukin 1 Receptor Antagonist Protein , Kinetics , Leukocytes, Mononuclear/cytology , Leukocytes, Mononuclear/metabolism , Neutrophils/drug effects , Superoxides/metabolism , Time Factors , Up-Regulation
5.
J Immunol ; 159(12): 6164-8, 1997 Dec 15.
Article in English | MEDLINE | ID: mdl-9550418

ABSTRACT

As the first line of defense in the immune system, neutrophils may release a variety of potent agents upon exposure to infectious agents. In this study we have investigated the ability of human neutrophils to produce chemotactic cytokines, or chemokine in response to EBV. Exposure of neutrophils to EBV led to an increase in accumulation of mRNA for IL-8 and macrophage inflammatory protein-1alpha (MIP-1alpha). EBV stimulated a time-dependent production of immunoreactive IL-8 and MIP-1alpha by neutrophils. The ability of EBV to stimulate the synthesis of IL-8 and MIP-1alpha protein was reflected by both an accumulation of the protein in the intracellular compartment as well as increased secretion. A variety of control studies support the idea that infectious EBV is not required for induction of chemokine gene expression; however, the response is dependent on the interaction between the glycoprotein gp350 of the viral envelope and the neutrophil surface. Since both IL-8 and MIP-1alpha are reported to be chemoattractants in vitro for T cells and for T and B cells, respectively, the ability of EBV to induce their production by neutrophils may enhance the ability of this virus to infect B and T lymphocytes via increased recruitment to sites of infection.


Subject(s)
Herpesvirus 4, Human/immunology , Interleukin-8/biosynthesis , Macrophage Inflammatory Proteins/biosynthesis , Neutrophils/metabolism , Chemokine CCL3 , Chemokine CCL4 , Cycloheximide/pharmacology , Gene Expression Regulation/drug effects , Herpesvirus 4, Human/physiology , Humans , Infectious Mononucleosis/blood , Infectious Mononucleosis/immunology , Infectious Mononucleosis/virology , Interleukin-8/blood , Interleukin-8/genetics , Kinetics , Macrophage Inflammatory Proteins/blood , Macrophage Inflammatory Proteins/genetics , Neutrophils/drug effects , Neutrophils/immunology , Neutrophils/virology , Phosphonoacetic Acid/pharmacology , RNA, Messenger/biosynthesis , RNA, Messenger/drug effects , Virus Activation/immunology
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