Novel polar lipids of halophilic eubacterium Planococcus H8 and archaeon Haloferax volcanii.

As part of a study to identify novel lipids with immune adjuvant activity, a structural comparison was made between the polar lipids from two halophiles, an archaeon Haloferax volcanii and a eubacterium Planococcus H8. H. volcanii polar lipid extracts consisted of 44% archaetidylglycerol methylphosphate, 35% archaetidylglycerol, 4.7% of archaeal cardiolipin, 2.5% archaetidic acid, and 14% sulfated glycolipids 1 and 2. Nuclear magnetic resonance (NMR) and Fast atom bombardment mass spectrometry (FAB MS) data determined the glycolipids to be 6-HSO(3)-D-Man(p)-alpha1-2-D-Glc(p)-alpha1,1-[sn-2,3-di-O-phytanylglycerol] and a novel glycocardiolipin 6'-HSO(3)-D-Man(p)-alpha1-2-D-Glc(p)-alpha1,1-[sn-2,3-di-O-phytanylglycerol]-6-[phospho-sn-2,3-di-O-phytanylglycerol]. The polar lipids of Planococcus H8 consisted of 49% saturated phosphatidylglycerol and cardiolipin (9:1, w/w), and surprisingly 51% of the photosynthetic membrane lipid sulfoquinovosyldiacylglycerol (SQDG). This study documents archaeal cardiolipin and a novel glycocardiolipin in H. volcanii (lacking purple membrane), and is the first report of SQDG in a non-photosynthetic, halophilic bacterium.

Construction of multivalent sialyl Le(x) from the type Ia group B Streptococcus capsular polysaccharide.

The type Ia group B Streptococcus (GBSIa) capsular polysaccharide was specifically degraded by partial Smith oxidation of 2,3-diol of the Glc in the backbone to fragments representing asialo core repeating units. Sialylation of these oligomers furnished GBSIa multiple repeating units. One, two and three repeating units of GBSIa were obtained pure, and the higher oligomers were obtained as mixtures. After enzymatic fucosylation oligosaccharides carrying bivalent, trivalent and other multivalent sialyl Le(x) epitopes presented as appendages on an oligolactoside scaffold were obtained.

Family-centered care in Ontario general hospitals: the views of pediatric nurses.

Registered nurses and registered practical nurses working on pediatric units in 35 Ontario general hospitals participated in a study that examined their perceptions and practices of family-centered care (FCC). The Family-Centered Care Questionnaire-Revised (FCCQ-R) was used to collect the data. The participants were asked to rate their level of agreement regarding necessary elements (perceptions) of family-centered care and whether these elements are part of their current work (practice). The participants had a reasonable knowledge of the necessary elements of family-centered care, but were not consistently including these in their every day work. A number of barriers to the implementation of family-centered care were also identified. Perception and practice scores were higher among those who had participated in continuing education on family-centered care than those who had not. The findings suggest that some nurses may be having difficulties shifting from a medical helping model of care to an enabling helping model of care, which is considered the foundation of family-centered care. Difficulties in implementing family-centered care appear to be systemic, both at the unit and organizational level. Based on the findings, implications for practice, education and research are suggested.

Synthesis and NMR assignments of galactosylgloboside and its beta-D-GalNAc-(1-->4)-alpha-D-Gal-linked positional isomer in a conjugatable form.

Two pentasaccharides suitable for conjugation, namely 3-aminopropyl glactosylgloboside and its beta-D-GalNAc-(1-->4)-alpha-D-Gal-linked positional isomer, were synthesized from 3III,4III-di-O-unprotected globotrioside and the trichloroacetimidate of beta-D-Gal-(1-->3)-beta-D-GalNPhth derivative. Glycosylation at both positions led to the formation of beta-D-GalNPhth-(1-->4)-alpha-D-Gal and beta-D-GalNPhth-(1-->3)-alpha-D-Gal-linked products in a ratio of 1:1 without selectivity. Complete NMR spectral assignments are also described.

[Measure of parental behavior during children's ambulatory surgery]. / Mesure des conduites parentales lors d'une chirurgie de l'enfant en court séjour.

A new instrument, l'Inventaire des conduites parentales, was validated by the main author during her doctoral studies. The purpose of her research was to measure the effect of an educational intervention on parent's behaviors. This instrument, based on an educational theoretical framework, was composed of 16 behaviors divided into 3 categories: verbal information, cognitive strategies and attitudes. The reliability of the initial version of the instrument was measured with a sample of 142 parents. The interrater agreement was of 94.3%. The factor analysis showed that 14 of the 16 items were closely related to 4 clusters of variables. Following the factor analysis results, and the utilization of this instrument by research assistants, it was revised and is now described in this article.

Distribution of adenosine A1 receptors in primary visual cortex of developing and adult monkeys.

Adenosine is an endogenous neuromodulator, whose actions in the brain are mediated by several, highly specific receptor systems. This study examined the distribution of adenosine A1 receptors in the primary visual cortex of developing and adult monkeys by way of radioligand-binding autoradiography. Receptor density was found to be highest in layers 2/3 and 5 of adult striate cortex and lowest in layer 4. A similar pattern was found in infant monkeys that were in the critical period of development. Furthermore, removal of visual stimulation appeared to have no effect on receptor density in any of the layers. These results show that levels of adenosine A1 receptors are not affected within ocular dominance columns after removal of visual input and that the geniculorecipient layer is less likely to be influenced by endogenous adenosine than other layers during early development and in the adult.

[A baccalaureate program born from a university and a college collaboration]. / Un baccalauréat né d'une collaboration entre une université et un collège.

Ontario health care reform has necessitated many changes in the nursing profession. To practice effectively, nurses must readjust their roles and training. This article discusses a revised bachelor of nursing program that is the result of collaboration between two teams of educators from the French-language division of the School of Nursing at Laurentian University and the Nursing Program at Collège Boréal in Sudbury, Ontario. It describes the preliminary stages of program development, the major players, the underlying philosophy and an overview of the program in each of the four years of study. The program, which includes the option of earning a college diploma at the end of the third year, is slated for introduction in the fall of 1997.

Activity-dependent regulation of cytochrome b gene expression in monkey visual cortex.

The recent demonstration that certain mitochondrial subunits of cytochrome oxidase (CO) are regulated by neuronal activity has stimulated interest in the molecular processes that coordinate nuclear and mitochondrial gene expression following synaptic stimulation. We have studied the constitutive expression and activity-guided regulation of cytochrome b (cyt b), a gene that is encoded by mitochondrial DNA and that was cloned by subtractive hybridization from the lateral geniculate nucleus in the monkey. We have found cyt b mRNA expression in monkey striate cortex to be similar to that of CO activity with regard to the laminar profile and the presence of blobs in the supragranular layers. Layers 2/3, 4C, and 6 contained large numbers of stained cells, many of which were judged to be excitatory neurons, because they showed a Zif268-immunopositive nucleus. We have also found that removal of functional activity reduced cyt b mRNA content in area V1. Columns of reduced cyt b staining were visible after 3 days and were especially striking after 6 days of monocular deprivation. After 3 months of deprivation, the columns lost their contrast and became blurred. Our principal finding, that neuronal activity regulates cyt b levels, suggests that expression of a mitochondrial gene can be affected in a manner similar to that of several known nuclear genes. The differences in cyt b mRNA levels and CO activity after long-term deprivation suggests that some form of differential control is exerted on cyt b. Cyt b expression, therefore, may be used as a marker of altered mitochondrial transcription that is guided by the metabolic demands of active neurons.

Dual activity maps in primate visual cortex produced by different temporal patterns of zif268 mRNA and protein expression.

The inducible nature of the immediate-early genes (IEGs) c-fos and zif268 allows their products to be used as activity markers in the brain. The utility of such markers in general is restricted because they can resolve only neurons activated by a single stimulus. To overcome this limitation, we have developed a double-label technique that exploits the dissimilar time course of zif268 mRNA and protein induction, allowing them to be separately induced by two different stimuli and independently stained to provide a visual display of neurons that are responsive to each stimulus. Two powerful features of this new imaging technique-the possibility of staining separate populations of activated neurons and the ability to visualize them at the cellular level-should extend IEG applications in biological activity mapping.

Graduating students' initial career plans.

As recent as six years ago, a shortage of nurses in Canadian hospitals meant that graduating nursing students had little difficulty finding work to begin their careers. But the realities of provincial budgetary deficits and extensive restructuring of the health care system have translated into downsizing of hospital staff, a shift to casual and part-time work, a loss of nursing positions, a surplus of RNs, and limited employment opportunities for graduating students. Today, things are slowly starting to turn around.

Neonatal endotoxin exposure alters the development of the hypothalamic-pituitary-adrenal axis: early illness and later responsivity to stress.

The long-term consequences of neonatal endotoxin exposure on hypothalamic-pituitary-adrenal axis (HPA) function were assessed in adult female and male Long-Evans rats. At 3 and 5 d of age, pups were administered endotoxin (Salmonella enteritidis, 0.05 mg/kg, i.p.) at a dose that provokes a rapid and sustained physiological response, but with no mortality. As adults, neonatally endotoxin-treated animals exhibited significantly greater adrenocorticotrophic hormone (ACTH) and corticosterone responses to restraint stress than controls. In addition, dexamethasone pretreatment was less effective in suppressing ACTH responses to restraint stress in endotoxin-treated animals than in controls, suggesting decreased negative-feedback sensitivity to glucocorticoids. Neonatal endotoxin treatment elevated resting-state median eminence levels of corticotropin-releasing hormone (CRH) and arginine vasopressin in adult male animals, and arginine vasopressin in both adult males and females. Neonatal exposure to endotoxin also increased CRH mRNA expression in the paraventricular nucleus of the hypothalamus of adult males, with no difference in females. Finally, glucocorticoid receptor density was reduced across a wide range of brain regions in the neonatal endotoxin-treated, adult animals. These data illustrate the interactive nature of immune and endocrine systems during development. It appears that endotoxin exposure during critical stages of development decreases glucocorticoid negative-feedback inhibition of ACTH secretagogue synthesis, thus increasing HPA responsiveness to stress. The implication of these findings is that exposure to gram-negative LPS in early life can alter the development of neural systems which govern endocrine responses to stress and may thereby predispose individuals to stress-related pathology.

Postnatal handling alters glucocorticoid, but not mineralocorticoid messenger RNA expression in the hippocampus of adult rats.

Postnatal handling alters hypothalamic-pituitary-adrenal (HPA) responses to stress in the rat. Handling also increases hippocampal glucocorticoid receptor density, and this effect appears to form, in part at least, the basis for the effect of handling on HPA responsiveness to stress. In the present study we have used in situ hybridization techniques to examine the effect of postnatal handling on the expression of glucocorticoid and mineralocorticoid receptor mRNAs in various cell fields of the dorsal hippocampus in adult rats. Grain counting analysis over individual cells showed that postnatal handling significantly increased (40-50%) glucocorticoid receptor mRNA in all hippocampal cell fields. In contrast, handling had no effect on mineralocorticoid receptor mRNA expression. These findings are consistent with the results of receptor binding studies showing that handling increases hippocampal glucocorticoid receptor, but not mineralocorticoid receptor density. Thus, the increase in glucocorticoid receptor binding in handled animals is likely associated with altered rates of receptor biosynthesis. Moreover, the handling effect is quite specific, altering glucocorticoid receptor, but not mineralocorticoid receptor mRNA expression. The mechanism(s) whereby glucocorticoid receptor gene expression is permanently increased by postnatal handling remains to be determined.

Molecular basis for the development of individual differences in the hypothalamic-pituitary-adrenal stress response.

1. Several years ago, investigators described the effects of infantile handling on the development of hypothalamic-pituitary-adrenal (HPA) responses to stress in the rat. Rat pups exposed to brief periods of innocuous handling early in life showed reduced HPA responses to a wide variety of stressors, and the effect persists throughout the life of the animal. These effects are robust and provide an excellent model for understanding how early environmental stimuli, which are external to the organism, alter neural differentiation and, thus, neuroendocrine responsivity to stress. 2. This paper reviews the endocrine mechanisms affected by early handling and our current understanding of the neural transduction of environmental events and their effects at the level of the target neurons (in the hippocampus and frontal cortex). 3. In brief, handling serves to increase glucocorticoid receptor gene transcription, increasing sensitivity to glucocorticoid negative feedback regulation and, thus, altering the activity within hypothalamic corticotropin-releasing factor/vasopressin neurons. Together these changes serve to determine neuroendocrine responsivity to stress.