ABSTRACT
Estudios clínicos y la función in vitro del neutrófilo fueron estudiados en 10 pacientes con acné vulgar y lesiones inflamatorias severas tratados con isotretinoína (134-cis-retinoide). Luego de 12 semanas de tratamiento se observó la mejoria clínica de las lesiones. Al inicio se comprobó en todos los pacientes una depresión de las funciones quimiotácticas, prueba NAT y actividad lisosómica, que fueron más evidentes cuando se inclcuyó suero autólogo en estas pruebas. Luego de 8 semanas de tratamiento la función quimiotáctica y la prueba NAT aumentaron significativamente. En cambio, la actividad lisosómica aumentó significativamente luego de 12 semanas de tratamiento. La mejoría de la función in vitro se evidencío también en las pruebas con suero autológo. Los autores sugieren que la isotretinoína posee múltiples acciones locales y sistémicas, en particular la disminución del Priopionibacterium acnes con la subsequente disminución de las respuestas inmunes específicas e inespecíficas
Subject(s)
Adolescent , Adult , Humans , Male , Female , Acne Vulgaris/drug therapy , Granulocytes/physiology , Tretinoin/therapeutic use , Acne Vulgaris/pathologyABSTRACT
Estudios clínicos y la función in vitro del neutrófilo fueron estudiados en 10 pacientes con acné vulgar y lesiones inflamatorias severas tratados con isotretinoína (134-cis-retinoide). Luego de 12 semanas de tratamiento se observó la mejoria clínica de las lesiones. Al inicio se comprobó en todos los pacientes una depresión de las funciones quimiotácticas, prueba NAT y actividad lisosómica, que fueron más evidentes cuando se inclcuyó suero autólogo en estas pruebas. Luego de 8 semanas de tratamiento la función quimiotáctica y la prueba NAT aumentaron significativamente. En cambio, la actividad lisosómica aumentó significativamente luego de 12 semanas de tratamiento. La mejoría de la función in vitro se evidencío también en las pruebas con suero autológo. Los autores sugieren que la isotretinoína posee múltiples acciones locales y sistémicas, en particular la disminución del Priopionibacterium acnes con la subsequente disminución de las respuestas inmunes específicas e inespecíficas (AU)
Subject(s)
Adolescent , Adult , Humans , Male , Female , Granulocytes/physiology , Acne Vulgaris/drug therapy , Tretinoin/therapeutic use , Acne Vulgaris/pathologyABSTRACT
Sixteen patients with acne vulgaris were studied. All patients had severe inflammatory lesions, nodulocystic and conglobata. Of them, 12 was male and 4 female, their age was between 16 and 24 years old. In all the cases laboratory test to evaluate chemotactic, nitroblue-tetrazolium and lysosomal activity of granulocyte were performed. The protocol included in vitro incubations with and without autologous serum for considered their action on the phagocytic cell. The results shows an inhibitory action of the autologous serum in chemotactic (d-mean, 34.25%; SEM, 2.39, p less than 0.01), nitroblue-tetrazolium test (d-mean, 21.06%; SEM, 2.85, p less than 0.05), and lysosomal activity (d-mean, 21.43%; SEM, 2.80, p less than 0.05). The authors suggest that granulocyte alterations that occur in inflammatory forms of acne vulgaris is a secondary phenomenon may be caused by seric IgE or vasoactive substances released during immune responses.
Subject(s)
Acne Vulgaris/blood , Chemotaxis, Leukocyte , Neutrophils/physiology , Adolescent , Adult , Female , Humans , MaleABSTRACT
Twenty seven patients with acne vulgaris were studied. Of them, 10 had minimal lesions and 17 had severe inflammatory lesions: nodulocystic, and conglobata. NBT and lysosomal neutrophil function was performed. The results shows a significative decreased values for the three test in patients with inflammatory lesions compared to patients with non-inflammatory lesions. The values for chemotactic function was 56.23 and 93%, for NBT test 50.82 and 93% and for lysosomal activity 60.88 and 88.9%, respectively. The authors proposed that the altered in vitro neutrophil function is a secondary phenomenon caused by specific and non-specific immunologic factors that occur in patients with severe inflammatory acne vulgaris.
Subject(s)
Acne Vulgaris/physiopathology , Chemotaxis, Leukocyte , Neutrophils/physiology , Adolescent , Adult , Female , Humans , MaleABSTRACT
The authors study three cytotoxicity system in psoriasis: direct cytotoxicity (DCC), antibodies mediated cytotoxicity (ADCC) and activated lymphocytes mediated cytotoxicity (PCC). The results of DCC and PCC are in the normal values, while ADCC are a somewhat increased, showing a hyperactivity of this system, which could explain some physiopathological phenomena of this disease. We consider this finding as secondary to an alteration of the immune system by dysbalance of B-T lymphocytes.
Subject(s)
Psoriasis/immunology , Adolescent , Adult , Cytotoxicity Tests, Immunologic , Cytotoxicity, Immunologic , Female , Humans , Male , Middle Aged , Monocytes/immunologyABSTRACT
By Hondius-Boldingh. Laurence and Bullough, Laurence method, the activity of the epidermal chalone receptors was studied in 36 psoriasis and 36 healthy controls. The lymphocyte chalone receptors was also studied in 26 patients, by Garcia Giralt et al. and Lasalvia et al. method. The results showed that the epidermal chalone receptors are significantly decreased in the involved skin, and in a lesser degree in the uninvolved one of the patients with psoriasis. The lymphocyte chalone receptors were normal. This decreasing in the function of the epidermal chalone system by means of different "antireceptor factors", interrupt the physiological cell control of the epidermis. The absence of the chalone specific function, produces an intracelular disbalance which provokes, in particular, the high number of mitosis and the lack of terminal differentiation. These elements admit that in psoriasis there is an abnormal epidermopoyesis. Over a genetic disorder, multiple factors leading to a deffect of the cell membrane. These factors, acquired, local or systemic interfering with the receptors of membrane, would alter the cellular regulation induced my the chalones.
Subject(s)
Growth Inhibitors , Lymphocytes/analysis , Psoriasis , Receptors, Cell Surface/analysis , Adolescent , Adult , Female , Humans , MaleABSTRACT
The epidermic chalone receptor of skin with lesion and without lesion are studied on 20 patients with active psoriasis. The testlest were performed before and two month after oral treatment with RO 10-9359. Roche. The results show significant variation with an increased a the number of receptors after treatment. As these receptors are in the cell membrane, the authors consider that RO 10-9359 action in on the membrane and its surrounding, making in possible to the epithelial cell to begin its autoregulation, autocontrol and differentiation when the specific activity of the chalones become normal.
Subject(s)
Growth Inhibitors , Psoriasis , Receptors, Cell Surface/analysis , Adolescent , Adult , Child , Etretinate/therapeutic use , Female , Humans , Male , Middle Aged , Psoriasis/drug therapy , Skin/analysisABSTRACT
The concentration of total cholesterol and that of its sterified fraction is studied in skin biopsies and blood of psoriatic patients. The results show high cholesterol levels in skin of psoriatic plaques and of healthy areas. The sterified cholesterol proportion is significantly less in plaques than in skin without lesions of the same patients, and that of the normal controls. These alterations are not accompanied by a modification of the plasmatic lipidic profile. The results could be the expression of a raise of the skin cholesterol preceeding and perhaps predisposing the evolution of the psoriatic lesion, and the deficit on the cholesterol enzymes sterification as a biochemical marker of the accelerated differentiation and cornification at the psoriatic plaque.
Subject(s)
Cholesterol Esters/analysis , Cholesterol/analysis , Lipoproteins, HDL/analysis , Psoriasis/metabolism , Skin/analysis , Cholesterol, HDL , Humans , Psoriasis/bloodABSTRACT
The circunlant antimelanin antibodies are studied in common or idiopathic vetiligo. The patients did not have other skin or systemic lesions. The results show the presence antimelanin antibodies in a very significant fashion. Based on these elements, the authors consider that this observation is very important and that in this disease the autoimmune mechanisms have a fundamental character. In vitiligo as in every autoimmune disease, the etiology can be multiple.
Subject(s)
Antibodies/analysis , Melanins/immunology , Vitiligo/immunology , Adolescent , Adult , Child , Female , Humans , Male , Middle AgedABSTRACT
--The authors study in psoriasis an enzyme of the glucolytic way, the Phospho-hexose-isomerase. --They find significant high levels in blood and damaged skin, while these were "normal" in healthy skin. --Phospho-hexose-isomerase acts as a biological marker in psoriasis, and acquires the significance of an active cellular proliferation. --The highest values are those of the erythrodermaes which become normal with clinical involution. --The authors suggest that psoriasis, by an exaggerated cellular division inhibition mechanism, metabolism is deviated in order to bring the energetic elements and substrate to form the new cell. --Phospho-hexose-isomerase is not specific of the malignant process.
Subject(s)
Glucose-6-Phosphate Isomerase/blood , Psoriasis/enzymology , Adolescent , Cell Division , Female , Humans , Male , Middle Aged , Phosphofructokinase-1/analysis , Psoriasis/pathology , Skin/enzymologyABSTRACT
Three cellular cytotoxic lymphocytary populations were studied in patients with different forms of inflammatory acne vulgaris: direct or spontaneous, antibodies dependent and phytohemagglutinine stimulated. To these was added "Propionibacterium acnes" from cultures of material extracted separately from comedones and pustules. The direct or spontaneous and antibodies dependent cytotoxicity were normal. The subpopulation responsible of the phytohemagglutinine stimulated cytotoxicity has shown a clear hyperactivity which was augmented by the addition of these bacteria. But this hyperactivity stimulation was selective for the "Propionibacterium acnes" from pustules and not for those from comedones. These findings could contribute to the knowledge of the immune cellular mechanisms in acne vulgaris inflammation provoked by these bacteria.
Subject(s)
Acne Vulgaris/immunology , Propionibacterium acnes/immunology , Acne Vulgaris/microbiology , Antibody-Dependent Cell Cytotoxicity , Cytotoxicity Tests, Immunologic/methods , Humans , Killer Cells, Natural/immunology , Lymphocytes/drug effects , Phytohemagglutinins/pharmacology , Propionibacterium acnes/isolation & purification , T-Lymphocytes/immunologyABSTRACT
In both cases, the interferon is significantly increased. This could explain the immunological alterations of the efferent or the afferent ways described in the psoriasis. The interferon anti-proliferative action would not be produced by a failure in the receptors of the cellular membrane at the psoriasis epidermis. In some psoriasis, interferon inductors activated or not by viruses could be associated to the etiology of the disease.
Subject(s)
Interferons/blood , Psoriasis/blood , Adolescent , Adult , Female , Humans , Leukocytes/analysis , Male , Middle AgedABSTRACT
Se estudian los niveles sericos de antiproteasas totales,antitripsina y antiquimiotripsina en pacientes con acne vulgaris inflamatorio. Las lesiones clinicas se clasificaron en dos grados: I-II con lesiones inflamatorias minimas y escasas secuelas y III-IV con lesiones muy inflamatorias pustulosas y abscedadas y multiples y evidentes cicatrices atroficas. Los resultados muestran que los niveles de antiproteasas totales, antitripsina y antiquimiotripsina fueron significativamente bajos en los grados III-IV en relacion con los de I-II. Es probable que las enzimas lisosomales proteoliticas neutras granulocitarias liberadas en el foliculo pilosebaceo durante la inflamacion, especialmente las elastasas y colagenasas no pueden ser inhibidas por las antiproteasas correspondientes del suero. Ello provoca un aumento del proceso inflamatorio y una mayor destruccion de las fibras elasticas y colagenas lo que conduce a cicatrices atroficas con ausencia de los tejidos normales epiteliales y conectivos
Subject(s)
Acne Vulgaris , alpha 1-Antitrypsin , Protease InhibitorsABSTRACT
Los autores estudian una enzima de la via glucolitica, la fosfohexosaisomerasa en la psoriasis. Los hallazgos demuestran una elevacion significativa en sangre y en piel enferma. Luego del tratamiento con Ro 10-9359 se obtuvo evidente mejoria clinica y normalizacion de los niveles de la fosfohexosaisomerasa. Esta enzima es un indice de actividad lesional de la psoriasis, correspondiendo los valores mas elevados a las formas eritrodermicas. Se plantea como probable mecanismo farmacobiologico del Ro 10-9359 su accion sobre la membrana celular, sus receptores u otras estructuras afines
Subject(s)
Glucose-6-Phosphate Isomerase , Psoriasis , TretinoinABSTRACT
Se estudian los niveles sericos de antiproteasas totales,antitripsina y antiquimiotripsina en pacientes con acne vulgaris inflamatorio. Las lesiones clinicas se clasificaron en dos grados: I-II con lesiones inflamatorias minimas y escasas secuelas y III-IV con lesiones muy inflamatorias pustulosas y abscedadas y multiples y evidentes cicatrices atroficas. Los resultados muestran que los niveles de antiproteasas totales, antitripsina y antiquimiotripsina fueron significativamente bajos en los grados III-IV en relacion con los de I-II. Es probable que las enzimas lisosomales proteoliticas neutras granulocitarias liberadas en el foliculo pilosebaceo durante la inflamacion, especialmente las elastasas y colagenasas no pueden ser inhibidas por las antiproteasas correspondientes del suero. Ello provoca un aumento del proceso inflamatorio y una mayor destruccion de las fibras elasticas y colagenas lo que conduce a cicatrices atroficas con ausencia de los tejidos normales epiteliales y conectivos
Subject(s)
Acne Vulgaris , alpha 1-Antitrypsin , Protease InhibitorsABSTRACT
Los autores estudian una enzima de la via glucolitica, la fosfohexosaisomerasa en la psoriasis. Los hallazgos demuestran una elevacion significativa en sangre y en piel enferma. Luego del tratamiento con Ro 10-9359 se obtuvo evidente mejoria clinica y normalizacion de los niveles de la fosfohexosaisomerasa. Esta enzima es un indice de actividad lesional de la psoriasis, correspondiendo los valores mas elevados a las formas eritrodermicas. Se plantea como probable mecanismo farmacobiologico del Ro 10-9359 su accion sobre la membrana celular, sus receptores u otras estructuras afines
Subject(s)
Glucose-6-Phosphate Isomerase , Psoriasis , TretinoinABSTRACT
63 patients with Pityriasis capitis (PC) "dandruff" and Seborrheic dermatitis (SD) as well as normal controls were studied. Antigenicity was checked "in vitro" by the lymphocytic transformation test. A significant transformation in the PC to P. ovale was observed while in SD it was to Stanphyloccocus and P. ovale. In view of results the authors suggest that there is a similar immunoalergic response in the host to those antigens. The clinic will differ according to the antigens. This is significant for a new local treatment and general.
Subject(s)
Antigens, Bacterial/immunology , Dermatitis, Seborrheic/immunology , Lymphocyte Activation , Malassezia/immunology , Pityriasis/immunology , Dermatitis, Seborrheic/microbiology , Female , Humans , Lymphocyte Activation/drug effects , Lymphocytes/immunology , Malassezia/isolation & purification , Male , Phytohemagglutinins/pharmacology , Pityriasis/microbiology , Staphylococcus/immunology , Streptococcus/immunologyABSTRACT
The physiopathological mechanisms of the different degrees of the Acne Vulgaris are reviewed. The observations show the important antigenic activity of the "Propionibacterium acnes" and the cellular immunity in the inflammation. An immunologic concept could explain this process.
Subject(s)
Acne Vulgaris/physiopathology , Propionibacterium acnes/immunology , Acne Vulgaris/etiology , Acne Vulgaris/immunology , Androgens/physiology , Antigens, Bacterial/immunology , Bacterial Infections , Humans , Phagocytosis , Propionibacterium acnes/enzymology , T-Lymphocytes/immunologyABSTRACT
Se estudia el metabolismo de estructuras encefalicas dopaminergicas, a traves de la dosificacion del acido homovanilico (HVA) en el liquido cefalo-raquideo (LCR) de 14 enfermos con Parkinson senil o arterioesclerotico. Se confirma que las concentraciones de HVA en tales muestras tienen un valor medio significativamente inferior al obtenido en 10 muestras de sujetos normales del mismo rango de edad. Algunos casos presentan valores que se encuentran en el rango normal. El tratamiento con L-Dopa durante 3 meses obtuvo mejorias clinicas en casi todos los casos que presentaban inicialmente niveles subnormales de HVA y que alcanzaron o sobrepasaron el nivel normal durante el tratamiento (8 9). Los casos con niveles inicialmente elevados o que mantuvieron concentraciones subnormales a pesar del tratamiento, fueron fracasos terapeuticos (05)
