ABSTRACT
The national reference Center for blood groups checked samples of reagents and devices used in France for a definitive verification of pretransfusion ABO tests performed at the patient's bedside, as defined by French health authority regulations. The results of an initial inquiry was published in 1991. The new study shows no significant improvement of the quality of reagents and devices. This is a major concern considering the importance of ABO incompatibility in severe hemolytic transfusion reactions.
Subject(s)
ABO Blood-Group System/analysis , Blood Banks/statistics & numerical data , Blood Group Incompatibility/diagnosis , Blood Grouping and Crossmatching/statistics & numerical data , Blood Grouping and Crossmatching/instrumentation , Blood Grouping and Crossmatching/methods , Blood Transfusion , Data Collection , Erythrocyte Transfusion , Evaluation Studies as Topic , Forms and Records Control , France , Humans , Indicators and Reagents , Medical Records , Reproducibility of Results , Safety , Blood Banking/methodsABSTRACT
In this paper we chose to emphasize three aspects of our work. First we underlined that "low grade and high grade" D weak red blood cells studied at the DNA level could, when monoclonal antibodies were used, give patterns of positive and negative reactions like partial RH1(D) cells. Secondly, we showed the importance of the technical conditions of the study which are essential for establishing a pattern of reactivity defining an epitope. It appears that the use of papain treated cells at room temperature can be misleading for the definition of epitope especially with IgM antibodies. Lastly we pointed out the interest of Rh variant cells, defined at the gene level, to study the expression of RH1(D) epitopes on the external part of the membrane.
Subject(s)
Antibodies, Monoclonal/immunology , Rh-Hr Blood-Group System/immunology , Humans , SerologyABSTRACT
The use of red blood cells coated with complement fractions using low ionic strength saline conditions is part of the evaluation of anti-complement antibodies, specially monoclonal antibodies used for antiglobulin reagents. This work shows that such cells stored in liquid nitrogen allow satisfactory long-term studies except for the anti-C3g antibodies.
