Toxicogenetic, biochemical, and anatomical effects of the herbicide Clethodim on Allium cepa L.

Pesticides are compounds with several chemical or biological agents developed to potentiate the biocide action. Their use is associated with increased economic and agricultural productivity worldwide but can harm health and the environment, damaging existing biota. Clethodim is a systemic post-emergent herbicide for grasses, highly selective for cotton, coffee, onions, carrots, soybeans, etc. Therefore, this work aimed to evaluate the harmful effect of the herbicide Clethodim with the model plant Allium cepa. A series of tests were conducted to evaluate the effects of the herbicide under study. Germination tests, root growth, cell, and nucleolar cycle analysis, as well as oxidative stress assessment and histological analysis of the roots, were performed. The results indicated that the herbicide demonstrated phytotoxicity, inhibiting germination at C1 (1.92 g/L) and C3 (0.84 g/L), and root growth at all concentrations, presenting mutagenicity at C1 (1.92 g/L) and C4 (0.24 g/L), evidenced by the increased frequency of micronuclei. In addition, changes were observed in the enzymatic activity of the enzymes catalase at concentrations C1 (1.92 g/L) and C2 (0.96 g/L) and ascorbate peroxidase at concentrations C1 (1.92 g/L), C2 (0. 96 g/L), and C3 (0.48 g/L) and in cell elongation at concentrations C1 (1.92 g/L) and C3 (0.48 g/L), demonstrated in histological analyses of the root apex.

Assessment of surface water using Allium cepa test and histological analysis in Rhamdia quelen.

This study aimed to assess the cytotoxic, genotoxic, and mutagenic potentials of water samples collected in the Alegre River Basin, located in a predominantly rural area with no sewage treatment facilities in the Espírito Santo State, Brazil, using Allium cepa test. Also, gills and liver of Rhamdia quelen, a common fish species of the region, were histologically analyzed. A semi-quantitative analysis was performed and a histopathological alterations index (HAI) was determined. Our findings indicated that the waters of this river basin were cytotoxic (mitotic index reduction) and/or genotoxic (chromosomal abnormalities induction). Mutagenicity (micronuclei induction) was not observed for any water sample. The values for HAI showed that the waters caused moderate histological alterations in R. quelen. Liver was more sensitive than gills. It is necessary to implement a sewage treatment system and raise awareness on inappropriate management and disposal of agrochemicals in order to allow the recovery of Alegre River.