ABSTRACT
Fungi and yeasts were cultivated on media prepared from Eucheuma carrageenan. The cultures were found to compare well with those grown on agar media.
ABSTRACT
The use of carrageenan from the red alga Eucheuma striatum as a possible substitute for bacteriological agar was investigated. The carrageenan medium was compared with several commercially available media in terms of both their physical properties and the colony characteristics of 16 microorganisms grown on the media.
ABSTRACT
Lymphotoxin is secreted by human lymphocytes stimulated with phytohemagglutinin in vitro. Combinations of DEAE-cellulose and Sephadex chromatography, acrylamide gel electrophoresis, and isoelectric focusing were used to purify lymphotoxin 2000- to 4000-fold; 15-25% of the activity has been recovered. Lymphotoxin appears to be a weakly charged molecule(s) of molecular weight about 90,000-100,000 that migrates in Pevikon block electrophoresis as a beta- or alpha2 globulin. It is a discrete molecule(s), because it is completely separable from medium serum proteins and carrier and phytohemagglutinin proteins. Isoelectric-focusing studies indicate that there may be a limited heterogeneity among lymphotoxin molecules.
