ABSTRACT
An efficient method is described to tritiate spiperone at high specific activity.
Subject(s)
Spiperone/chemistry , Tritium/chemistry , Catalysis , Magnetic Resonance Spectroscopy/methods , Radioligand Assay , Spectrometry, Mass, Electrospray IonizationABSTRACT
Methods are presented to synthesize and characterize [methylene-3H] haloperidol 2 and [N-methyl-3H]trifluoperazine 6.
Subject(s)
Antipsychotic Agents/chemical synthesis , Haloperidol/chemical synthesis , Trifluoperazine/chemical synthesis , Tritium/chemistry , Haloperidol/analogs & derivatives , Magnetic Resonance SpectroscopyABSTRACT
Methods are presented to synthesize and characterize [N-methyl-3H] imipramine 3, [N-methyl-3H] 3-cyanoimipramine 6 and [N-methyl-3H] 2-nitroimipramine 9.
Subject(s)
Antidepressive Agents, Tricyclic/chemical synthesis , Imipramine/analogs & derivatives , Radiopharmaceuticals/chemical synthesis , Tritium/chemistry , Antidepressive Agents, Tricyclic/chemistry , Imipramine/chemical synthesis , Imipramine/chemistry , Radiopharmaceuticals/chemistryABSTRACT
Gamma-aminobutyric acid was labelled with tritium by the tritium gas catalytic reduction of a convenient 4-aminotetrolic acid derivative. The purified product was characterized by HPLC and tritium NMR.
Subject(s)
Tritium/chemistry , gamma-Aminobutyric Acid/chemistry , Aminobutyrates/chemistry , Catalysis , Chromatography, High Pressure Liquid , Isotope Labeling , Magnetic Resonance Spectroscopy/methods , Oxidation-Reduction , gamma-Aminobutyric Acid/metabolismABSTRACT
To study its mechanism of antimalarial action, a tritium labelled analogue of (+/-)-chloroquine was required at high specific activity. Two synthetic methods were successfully employed. [3-3H] (+/-)-Chloroquine 2 was prepared by the catalytic tritium dehalogenation of an iodo precursor and [N-ethyl-3H] (+/-)-chloroquine 4 was synthesized by the alkylation of (+/-)-desethylchloroquine with [3H] ethyl iodide.