[Effect of human somatotropin on metabolism in hypothalamo-hypophyseal nanism patients]. / Vliianie somatotropina cheloveka na obmen veshchestv u bol'nykh gipotalamo-gipofizarnym nanizmom.

The effect of pharmacopoeic human somatotropin (HS) produced by the Kaunas plant of endocrine preparations, on the proteic, lipid, carbohydrate and mineral metabolism was studied in 40 patients with hypothalamohypophyseal nanism (HHN). The experiments were performed before treatment on an empty stomach, 4 hs after HS administration in a dose of 4 I. U. and during the course of treatment (with 1-2 or 3-4 monthly intervals). The HS administration resulted in the increase of urea, phosphorus and alkaline phosphatase levels, decreased those of beta-lipoproteins, potassium and sodium and caused the redistribution of proteic fractions in the blood of HHN patients. Simultaneous reduction of the urea, creatinine and phosphoric excretion in lime with enhanced urinary excretion of potassium was observed. During a long-term intermittent HS therapy no changes were revealed in carbohydrate metabolism in HHN patients.

[Insulin-like substance and insulin-degrading complex in hemolysates of human erythrocytes]. / Insulinopodobnoe veshchestvo i insulindegradiruiushchii kompleks gemolizata éritrotsitov cheloveka.

Human erythrocyte lysate was fractionated on various gel filtration media and immunoreactive insulin, insulinase and the influence of individual fractions of the insulin-degrading activity were determined. The hemolysate was shown to contain a complex of substances including an insulin-like substance, insulinase, protease inhibitor and insulinase activator. The insulin-like substance eluted from a Sephadex G-50 column in the same manner as native insulin, and its concentration exceeded the plasma level. Insulinase (Mr 100,000) degraded insulin to the trichloroacetic acid soluble fragments but did not degrade protein or glycoprotein hormones from human pituitaries. Insulinase was inhibited by low temperature, aprotinin and by a newly discovered protease inhibitor from erythrocytes which also inhibits serine proteases--trypsin and chymotrypsin. Another newly discovered substance eluted from a Sephadex G-100 column in the region of low molecular weight substances and showed an insulinase activating activity. The elution patterns of the protease inhibitor and insulinase activator suggest the possibility of the presence of more than one inhibiting and activating factor. The experimental results suggest that the insulin-degrading complex plays a role of a regulator of plasma insulin level. The nonpancreatic origin of the insulin-like substance is also possible.

[Clinical use of Soviet-made human somatotropin preparation. 1. Dynamics of physical development]. / Opyt klinicheskogo primeneniia otechestvennogo preparata somatotropina cheloveka. Soobshchenie 1. Dinamika fizicheskogo razvitiia.

Human somatotropin preparation made in the USSR was used under clinical conditions. Treatment was instituted to 28 patients with various forms of nanism; observation period--up to 18 months. The preparation proved to be effective in hyposomatotropic hypophysial and cerebral nanism.