ABSTRACT
The Drosophila melanogaster P-transposable element is an example of mobile DNA transferred horizontally and known to have spread globally over the last 50-60 years. In Drosophila, the P-element causes a syndrome known as 'P-M hybrid dysgenesis' that obstructs normal ovary development in the female progeny of susceptible populations. Despite extensive research, the stability and global population dynamics of P-M dysgenic phenotypes remain poorly understood. Here, we report a recent and rapid transition in the P-M status of D. melanogaster populations from Ukraine. We demonstrate that these populations are currently dominated by the P'-cytotype characterized by active genomic P-elements and unknown from Ukraine just two decades ago. Our results suggest a recent invasion of the P-element in Ukraine, a pattern that matches recent discoveries from Turkey.
Subject(s)
DNA Transposable Elements , Drosophila melanogaster/genetics , Animals , Fertility/genetics , Genetics, Population , Hybridization, Genetic , Phenotype , UkraineABSTRACT
Two small temporal RNAs (stRNAs), lin-4 and let-7, control developmental timing in Caenorhabditis elegans. We find that these two regulatory RNAs are members of a large class of 21- to 24-nucleotide noncoding RNAs, called microRNAs (miRNAs). We report on 55 previously unknown miRNAs in C. elegans. The miRNAs have diverse expression patterns during development: a let-7 paralog is temporally coexpressed with let-7; miRNAs encoded in a single genomic cluster are coexpressed during embryogenesis; and still other miRNAs are expressed constitutively throughout development. Potential orthologs of several of these miRNA genes were identified in Drosophila and human genomes. The abundance of these tiny RNAs, their expression patterns, and their evolutionary conservation imply that, as a class, miRNAs have broad regulatory functions in animals.
Subject(s)
Caenorhabditis elegans/genetics , Gene Expression Regulation , RNA, Helminth/chemistry , RNA, Helminth/genetics , RNA, Untranslated/genetics , Animals , Base Sequence , Blotting, Northern , Cloning, Molecular , Conserved Sequence , Endoribonucleases/metabolism , Gene Expression Regulation, Developmental , Genes, Helminth , Genome , Humans , Molecular Sequence Data , Multigene Family , Nucleic Acid Conformation , RNA Precursors/genetics , RNA Precursors/metabolism , RNA, Helminth/physiology , RNA, Untranslated/chemistry , RNA, Untranslated/physiology , Ribonuclease III , Transcription, GeneticABSTRACT
Under diagnosis-related group prospective payment, the role of the hospital pharmacy department is to develop and promote cost effective and rational drug therapy. This study, conducted in a simulated fashion, evaluated the cost effectiveness of various cimetidine drug delivery systems: oral, nasogastric, and intramuscular. The evaluation was also extended to compare time efficacy between different dosage forms for each route of administration with the exception of the intramuscular route. Each of four nurses and four pharmacy technicians conducted 10 trials for each system to detect a statistical significance difference in pharmacy preparation and nursing administration time with at least 80% statistical power. The results showed no statistically significant difference (P > 0.1) between the total oral administration time of a unit-dose tablet or liquid. A significant difference was detected among the pharmacy-prepared liquids, and unit-dose liquid administered nasogastrically (P < 0.01). The most cost-effective system is the orally administered unit-dose tablet and the most expensive system is the unit-dose liquid administered nasogastrically.
