Subject(s)
Hernia, Inguinal/pathology , Mesothelioma, Cystic/pathology , Peritoneal Neoplasms/pathology , Pseudomyxoma Peritonei/pathology , Aged , Diagnosis, Differential , Hernia, Inguinal/complications , Hernia, Inguinal/surgery , Humans , Male , Peritoneal Neoplasms/complications , Peritoneal Neoplasms/surgery , Pseudomyxoma Peritonei/complications , Pseudomyxoma Peritonei/surgeryABSTRACT
We have determined the mass spectra of three polypeptides which have been designed as an effective reagent to be used in the diagnosis of a Chinese strain of Hepatitis E Virus (HEV). The spectra were determined by electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometer. These are peptide 30, peptide 33 and peptide 42, where the guanidine amino group of arginine in each polypeptide was protected. The molecular weights were respectively measured as 3575, 4094 and 5390 Dalton, against the molecular weights of bovine serum albumin (66430 Da) and bovine insulin (5732.77 Da).
Subject(s)
Peptides/analysis , Viral Proteins/analysis , Amino Acid Sequence , Cyclotrons , Fourier Analysis , Hepatitis E virus/chemistry , Mass Spectrometry , Molecular Sequence DataABSTRACT
The growth and differentiation of L6 myoblasts are subject to control by two proteins secreted by cells of the Buffalo rat liver line. The first of these, rat insulinlike growth factor-II (formerly designated multiplication stimulating activity) is a potent stimulator of myoblast proliferation and differentiation, as well as associated processes such as amino acid uptake and incorporation into protein, RNA synthesis, and thymidine incorporation into DNA. In addition, this hormone causes a significant decrease in the rate of protein degradation. All of these actions seem to be attributable to a single molecular species, although their time courses and sensitivity to the hormone differ substantially. The second protein, the differentiation inhibitor (DI), is a nonmitogenic inhibitor of all tested aspects of myoblast differentiation, including fusion and the elevation of creatine kinase. Indirect immunofluorescence experiments demonstrated that DI also blocks accumulation of myosin heavy chain and myomesin. Upon removal of DI after 72 h incubation, all of these effects were reversed and normal myotubes containing the usual complement of muscle-specific proteins were formed. Thus, this system makes it possible to achieve specific stimulation or inhibition of muscle cell differentiation by addition of purified proteins to cloned cells in serum-free medium.
