ABSTRACT
Acetobacter methanolicus MB 70 was shown to be related to the type strain of this species MB 58/4 (IMET 10945) having the same galactan-->2)-beta-D-Gal f-(1-->3)-beta-D-Gal p-(1-->as the capsular polysaccharide (CPS) and the O-side-chain of the lipopolysaccharide (LPS). Additionally, a glucan built up of the disaccharide repeating unit-->6)-alpha-D-Glc p-(1-->2)-alpha-D-Glc p-(1-->was identified in strain MB 70. In the CPS, the polymers were present in the ratio approximately 1:1, whereas the glucan preponderated in the LPS. Bacteriophage Acm6 specific to A. methanolicus MB 70 hydrolysed selectively the glucan component of both CPS and LPS. Structural elucidation of the resulting oligosaccharides led to the identification of the phage-associated depolymerase as an endo-alpha-(1-->6)-D-glucopyranoside hydrolase.
Subject(s)
Acetobacter/chemistry , Bacteriophages/metabolism , Lipopolysaccharides/chemistry , Oligosaccharides/chemistry , Polysaccharides, Bacterial/chemistry , Acetobacter/metabolism , Carbohydrate Conformation , Carbohydrate Sequence , Chromatography, Gel , Chromatography, Ion Exchange , Galactose/analysis , Glucose/analysis , Lipopolysaccharides/isolation & purification , Lipopolysaccharides/metabolism , Magnetic Resonance Spectroscopy , Methylation , Molecular Sequence Data , O Antigens , Oligosaccharides/isolation & purification , Polysaccharides, Bacterial/isolation & purification , Polysaccharides, Bacterial/metabolismSubject(s)
Acetobacter/chemistry , Lipopolysaccharides/chemistry , Oligosaccharides/chemistry , Polysaccharides, Bacterial/chemistry , Carbohydrate Conformation , Carbohydrate Sequence , Galactose/analysis , Glucose/analysis , Heptoses/analysis , Lipopolysaccharides/isolation & purification , Magnetic Resonance Spectroscopy , Mannose/analysis , Molecular Sequence Data , O Antigens , Oligosaccharides/isolation & purification , Optical Rotation , Polysaccharides, Bacterial/isolation & purification , Rhamnose/analysisABSTRACT
The capsular polysaccharide (CPS) and the O-side-chain of the lipopolysaccharide (LPS) of Acetobacter methanolicus MB 58/4 (IMET 10945) have been shown to contain the same disaccharide repeating unit, namely, ----2)-beta-D-Galf-(1----3)-beta-D-Galp-(1----. Degradation of the CPS and the LPS with the bacteriophage Acml gave fragments built up of 1-5 repeating units; the octasaccharide preponderated. The phage-associated depolymerase proved to be a beta-D-galactofuranoside hydrolase.
