[Neurophysiologic and histopathologic correlates of the effect of deferoxamine on the formation of an epileptic focus during blood injection into the rat cerebral cortex]. / Neirofiziologicheskie i gistopatologischeskie korreliaty deistviia deferoksamina na formirovanie épilepticheskogo ochaga u krys pri in"ektsii krovi v koru golovnogo mozga.

Blood, 5 microliters, was injected into the brain cortex of 80 adult male Wistar rats weighing 200-250 g. The animals were randomly divided into 2 groups: (1) control and (2) experimental. The second group was used to investigate the effects of deferoxamine on the development of epileptic activity and histopathological changes. The drug was injected intramuscularly in a daily dose of 80 mg/kg during 30 days after subdural blood injection. Epileptic activity appeared on day 2 after operation. The number of epileptic discharges was increased and reached the maximum values on days 3-10. Significant changes were not observed in the following period. In the experimental animals, the frequency of epileptic discharges on day 2 was comparable with those seen in the control group. A significant decrease in epileptic discharges occurred on day 4 following the surgery. Spike-waves disappeared on day 10 of the drug injection and only single spikes were registered on day 30. The histopathological analysis revealed a massive accumulation of pigments in the necrotic zone in the animals on day 5 after operation in both groups. The above changes were shown to retain in control animals on day 30. A negative response to the pigments was obtained only after 60 days. In contrast, responses to the pigment appeared to be negative on day 15 after the operation.(ABSTRACT TRUNCATED AT 250 WORDS)

[Amplification of the erbb-2 (Her-2/NEU), erbb-1 (HER-1) and c-myc oncogenes is often combined with the deletion of the short arm of chromosome 17 in human carcinoma]. / Amplifikatsiia onkogenom erbb-2 (HER-2/NEU), erbb-1 (HER-1) i c-myc chasta sochetaetsia s deletsiei korotkogo plecha khromosomy 17 v kartsinomakh u chelovekakh.

Amplification of oncogenes erbb-2, erbb-1, c-myc and losses of heterozygosity (LOH) at chromosomes 11p (probe hras-1), 17p (probe ynz-22) and 17q (probe thh-59) were studied in 165 human tumours (60 breast, 22 ovary, 40 colorectal, 23 lung, and 20 thyroid carcinomas). The correlation (P < 0.01) between the increased copy number of mentioned oncogenes and LOH at 17p was demonstrated for tumours tested: extra copies of these oncogenes were revealed in 11 of 46 DNA specimens with LOH on ynz-22, but only in 3 of 61 without LOH. This correlation was mostly due to frequent combinations between erbb-2 amplification and 17p deletions; the incidence of increased copy number of erbb-1 and c-myc oncogenes was not high enough for final conclusions about the association of their alterations with LOH at chromosome 17p.