ABSTRACT
The paper deals with the influence of sweeteners and aromatic compounds to the oxidative phosphorylation in rat liver mitochondria. The effect of food additives depends from its concentrations and time action.
Subject(s)
Food Additives/administration & dosage , Mitochondria, Liver/metabolism , Animals , Odorants , Oxidative Phosphorylation , Rats , Sweetening Agents/administration & dosageABSTRACT
Oxidative phosphorylation in the liver mitochondria of new-born and adult rats has been studied as affected by nitrates. It was established that the conjugation between the processes of respiration and phosphorylation is disturbed, energy efficiency of respiration in the liver mitochondria decreases in the new-born and adult animals. The found disturbances are more distinct in the new-borns that is evidenced by the dose-dependent effect under nitrate intoxications established in this group of animals.
Subject(s)
Aging/metabolism , Mitochondria, Liver/drug effects , Nitrates/toxicity , Oxidative Phosphorylation/drug effects , Animals , Animals, Newborn , Dose-Response Relationship, Drug , Oxygen Consumption/drug effects , RatsABSTRACT
Animals received saccharol adequate (by sweetness) to sugar, and in 10- and 50-fold increased amounts. The data of chronic 10-month experiments studied in the time course after 2, 5, 10 months have permitted a conclusion that saccharol included into the ration during long periods, does not produce a significant effect on the parameters of metabolic processes and morphological picture of the internal organs in test animals. Saccharol in 50-fold increased amounts inhibits the activity of oxidative phosphorylation in the hepatic tissue of rats.
Subject(s)
Liver Glycogen/metabolism , Mitochondria, Liver/drug effects , Models, Biological , Plant Extracts/pharmacology , Plants, Medicinal , Saccharin/pharmacology , Sweetening Agents/pharmacology , Animal Feed , Animals , Blood Glucose/analysis , Dose-Response Relationship, Drug , Mitochondria, Liver/metabolism , Oxidative Phosphorylation/drug effects , Plant Extracts/administration & dosage , Rats , Saccharin/administration & dosage , Sweetening Agents/administration & dosageABSTRACT
The influence of new exopolysaccharide, synthesized by Candida tropicalis and Pseudomonas sp. consortium in ethanol, on the animal body was studied in chronic experiments conducted in 200 non-inbred white rats. The animals were sacrificed after 3, 10 and 18 months of follow-up. The results of the investigations permit a conclusion that exopolysaccharide given to the animals during a long period does not produce a significant effect on the animal's growth and development, on the antitoxic function of the liver, on the parameters of protein, lipid and carbohydrate metabolism, on oxidizing phosphorylation in the mitochondria of the liver, and is low-hazard with respect to allergenic properties.
Subject(s)
Body Weight/drug effects , Candida/metabolism , Liver/metabolism , Polysaccharides, Bacterial/pharmacology , Polysaccharides/pharmacology , Pseudomonas/metabolism , Administration, Oral , Animal Feed , Animals , Biotransformation/drug effects , Biotransformation/physiology , Body Weight/physiology , Liver/drug effects , Oxidative Phosphorylation/drug effects , Polysaccharides/administration & dosage , Polysaccharides/biosynthesis , Polysaccharides, Bacterial/administration & dosage , Polysaccharides, Bacterial/biosynthesis , RatsABSTRACT
Experimental rats were given rations with 100% substitution of fats for phospholipid concentrate, during 1 and 3 months. It was found that such rations produced hypolipidemic and hypocholesterolemic effects, that were most pronounced at the end of the experiment. A control ration with sunflower oil containing high levels of unsaturated fatty acids induced accumulation of lipid peroxides in the hepatic tissue and blood plasma of the animals fed with the ration during 3 months. The ration containing the phospholipid concentrate did not produce such an effect. The data obtained have evidenced a positive influence of the phospholipid concentrate on the lipid metabolism parameters studied.
Subject(s)
Dietary Fats/administration & dosage , Lipid Metabolism , Mitochondria, Liver/drug effects , Oxidative Phosphorylation/drug effects , Phospholipids/administration & dosage , Animals , Blood Proteins/metabolism , Dietary Fats, Unsaturated/administration & dosage , Female , Lipid Peroxides/metabolism , Mitochondria, Liver/metabolism , Phospholipids/isolation & purification , Plant Oils/administration & dosage , Rats , Glycine max , Sunflower Oil , Time FactorsABSTRACT
The biological value of beef and pork of the animals implanted with antithyroid preparations did not differ from that of controls in varying tests. A long-term feeding of rats with diets containing meat of young bulls and pigs, implanted with betazine and diiodotyrosine, did not influence the content of free and bound cholesterol and total phospholipids in the liver, and produced no effect on the intensity of oxidative phosphorylation in the mitochondria which was proved by the results of the morphological, histochemical and ultrastructural investigations.
Subject(s)
Antithyroid Agents/pharmacology , Diiodotyrosine/analogs & derivatives , Diiodotyrosine/pharmacology , Meat , Animals , Body Burden , Cattle , Drug Implants , Liver/drug effects , Liver/metabolism , Male , Nutritive Value/drug effects , Rats , Swine , Time FactorsABSTRACT
A study was made of the effect of sugar beet saponins on motor activity of animals, energy, lipid and carbohydrate metabolism, osmotic resistance of red blood cells, antitoxic liver function. The 50- and 100-fold increase of the initial dose gave rise to an elevation of motor activity, lowering of the content of pre-beta-lipoproteins and increase of the amount of alpha-lipoproteins in blood serum, reduction of the osmotic resistance of red blood cells, dissociation of the processes of oxidation and phosphorylation, and decline of the energy efficacy of respiration.
Subject(s)
Plants, Edible , Saponins/pharmacology , Animals , Dose-Response Relationship, Drug , Lipoproteins/blood , Mitochondria, Liver/drug effects , Mitochondria, Liver/metabolism , Motor Activity/drug effects , Osmotic Fragility/drug effects , Oxidative Phosphorylation/drug effects , Rats , Time FactorsABSTRACT
It is established that sodium fluoride inhibits the activity of isolated soluble dehydrogenase and succinate dehydrogenase in the rat liver mitochondria in experiments in vitro. The degree of inhibition depends on the fluoride concentration and period of action. At the same time no changes are observed in the NADH-dehydrogenase activity of the mitochondria in the presence of 100 mM of sodium fluoride. With the intragastric administration of 35 and 24 mg/kg sodium fluoride to animals for 3 and 60 days, respectively, the succinate and NADH-dehydrogenase activities of the rat liver mitochondria lower significantly.
Subject(s)
Cytochrome Reductases/metabolism , Fluorides/pharmacology , Mitochondria, Liver/enzymology , NADH Dehydrogenase/metabolism , Sodium Fluoride/pharmacology , Succinate Dehydrogenase/metabolism , Animals , Kinetics , Male , RatsABSTRACT
This paper deals with studying ultrastructural changes in hepatocytes of rats caused by long introduction of sodium fluoride separately and in combination with glutamine, glutaminic acid and riboflavin. Sodium fluoride promotes the development of significant changes in hepatocytes ultrastructure. An attempt was made to reduce effect of the long-period influence of sodium fluoride by means of preliminary introduction of some agents...
