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1.
J Vet Cardiol ; 36: 186-190, 2021 Aug.
Article in English | MEDLINE | ID: mdl-34304107

ABSTRACT

A 10-year-old female Labrador retriever was presented for evaluation and management of syncope. On presentation, idioventricular tachycardia (IT) was electrocardiographically evident but neither ventricular capture nor fusion complexes were recorded. Spontaneous termination of the IT resulted in prolonged ventricular asystole and revealed the underlying rhythm of third-degree atrioventricular block. The serum concentration of troponin-I was elevated, suggesting myocarditis, but this was not diagnostically confirmed. It is likely that overdrive suppression of subsidiary idioventricular pacemakers, and perhaps also disease of subsidiary ventricular pacemaker fibers, importantly contributed to the duration of asystole.


Subject(s)
Arrhythmias, Cardiac , Atrioventricular Block , Dog Diseases , Animals , Arrhythmias, Cardiac/etiology , Arrhythmias, Cardiac/veterinary , Atrioventricular Block/complications , Atrioventricular Block/diagnosis , Atrioventricular Block/veterinary , Dog Diseases/diagnosis , Dogs , Electrocardiography/veterinary , Female , Heart Rate , Tachycardia/veterinary
2.
Epilepsy Behav ; 59: 13-20, 2016 06.
Article in English | MEDLINE | ID: mdl-27084978

ABSTRACT

OBJECTIVE: The aim of this study was to evaluate long-term safety, efficacy, and quality of life (QOL) of ≤400-mg/day USL255, Qudexy® XR (topiramate) extended-release capsules, as adjunctive therapy for partial-onset seizures (POS) in adults. METHODS: Patients who completed the 11-week double-blind treatment phase of the phase 3 PREVAIL study were eligible to enroll in this 1-year open-label extension (OLE) study (PREVAIL OLE). The primary objective was to evaluate the safety and tolerability of USL255 (including treatment-emergent adverse events [TEAEs]). The secondary objective was to assess seizure frequency in patients (e.g., median percent reduction from baseline in weekly POS frequency, responder rate [proportion of patients with ≥25%, ≥50%, ≥75%, or 100% reduction from baseline in POS frequency], and seizure-free intervals [proportion of patients who were seizure-free for 4, 12, 24, 36, or 48weeks]). Exploratory clinical-status endpoints included the Global Impression of Change (CGI-C) and Quality of Life in Epilepsy-Problems (QOLIE-31-P) questionnaires. Post hoc analyses evaluated neurocognitive TEAE incidences during the first 11 and entire 55weeks of treatment and efficacy by patient age and drug-resistant status. RESULTS: Of the 217 patients who completed PREVAIL (USL255, n=103; placebo, n=114), 210 (97%) enrolled in PREVAIL OLE and were included in the ITT population. Across the entire 55-week treatment period, USL255 was generally safe and well tolerated, with low individual neurocognitive TEAE incidences. Seizure reduction was sustained across the year-long study and observed in patient subgroups, including those with highly drug-resistant seizures and those ≥50years of age. Improvements in CGI-C and QOLIE-31-P were also observed. SIGNIFICANCE: The results of PREVAIL OLE are consistent with those from PREVAIL and demonstrate that adjunctive treatment with up to 400mg/day of USL255 may be a safe and effective treatment option for a variety of adult patients with refractory POS.


Subject(s)
Anticonvulsants/adverse effects , Anticonvulsants/therapeutic use , Drug Resistant Epilepsy/drug therapy , Epilepsies, Partial/drug therapy , Fructose/analogs & derivatives , Seizures/drug therapy , Adult , Aging , Anticonvulsants/administration & dosage , Cognition Disorders/chemically induced , Cognition Disorders/psychology , Delayed-Action Preparations , Double-Blind Method , Drug Resistant Epilepsy/psychology , Female , Fructose/administration & dosage , Fructose/adverse effects , Fructose/therapeutic use , Humans , Male , Quality of Life , Seizures/psychology , Surveys and Questionnaires , Topiramate , Treatment Outcome
3.
Geobiology ; 10(5): 457-66, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22726612

ABSTRACT

Water chemistry, energetic modeling, and molecular analyses were combined to investigate the microbial ecology of a biofilm growing in a thermal artesian spring within Hot Springs National Park, AR. This unique fresh water spring has a low dissolved chemical load and is isolated from both light and direct terrestrial carbon input - resulting in an oligotrophic ecosystem limited for fixed carbon and electron donors. Evaluation of energy yields of lithotrophic reactions putatively linked to autotrophy identified the aerobic oxidation of methane, hydrogen, sulfide, ammonia, and nitrite as the most exergonic. Small subunit (SSU) rRNA gene libraries from biofilm revealed a low-diversity microbial assemblage populated by bacteria and archaea at a gene copy ratio of 45:1. Members of the bacterial family 'Nitrospiraceae', known for their autotrophic nitrite oxidation, dominated the bacterial SSU rRNA gene library (approximately 45%). Members of the Thaumarchaeota ThAOA/HWCGIII (>96%) and Thaumarchaeota Group I.1b (2.5%), which both contain confirmed autotrophic ammonia oxidizers, dominated the archaeal SSU rRNA library. Archaea appear to dominate among the ammonia oxidizers, as only ammonia monooxygenase subunit A (amoA) genes belonging to members of the Thaumarchaeota were detected. The geochemical, phylogenetic, and genetic data support a model that describes a novel thermophilic biofilm built largely by an autotrophic nitrifying microbial assemblage. This is also the first observation of 'Nitrospiraceae' as the dominant organisms within a geothermal environment.


Subject(s)
Archaea/isolation & purification , Bacteria/isolation & purification , Biofilms/growth & development , Biota , Hot Springs/microbiology , Nitrification , Archaea/classification , Archaea/metabolism , Arkansas , Bacteria/classification , Bacteria/metabolism , Cluster Analysis , DNA, Archaeal/chemistry , DNA, Archaeal/genetics , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Genes, rRNA , Molecular Sequence Data , Oxidoreductases/genetics , Phylogeny , RNA, Archaeal/genetics , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid
4.
J Am Assoc Lab Anim Sci ; 48(5): 486-91, 2009 Sep.
Article in English | MEDLINE | ID: mdl-19807968

ABSTRACT

This study was conducted to investigate the possible effect of rack type on the blastocyst yield of mouse embryo donors. The first phase of the study consisted of housing some mice (group A) in a ventilated rack and others (group B) in a static rack in the same room for 3 d, followed by euthanasia for blastocyst collection and corticosterone assay. Parametric tests were used to compare groups. The number of blastocysts per donor was lower in group A (5.0 +/- 1.4 blastocysts) than group B (13.1 +/- 3.7 blastocysts). Mean noise was higher in the ventilated rack (80.4 dBC) than in the static rack (69.2 dBC). Serum corticosterone concentrations did not differ between groups. For the second phase of the study, a third group of mice (group C) was housed in a static rack without a ventilated rack in the same room. The noise level for group C was even lower (45.18 +/- 2.91 dBC), and the blastocyst count per donor (16.4 +/- 2.4) was higher than that of group B. The mean noise levels of empty ventilated and static racks differed significantly between groups for 10 different sound frequencies. Plotting mean blastocyst production against mean rack noise revealed a negative linear relationship with good strength of correlation. These results support the earlier observation that decreased blastocyst count occurs following housing of bred C57BL/6 donor mice in ventilated cages.


Subject(s)
Blastocyst/cytology , Housing, Animal , Mice/embryology , Noise , Animals , Corticosterone/blood , Female , Laboratory Animal Science , Mice, Inbred C57BL , Ventilation
5.
Neurochem Res ; 34(8): 1355-62, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19205880

ABSTRACT

S100beta is a soluble protein released by glial cells mainly under the activation of the 5-HT1A receptor. It has been reported as a neuro-trophic and -tropic factor that promotes neurite maturation and outgrowth during development. This protein also plays a role in axonal stability and the plasticity underlying long-term potentiation in adult brains. The ability of S100beta to rapidly regulate neuronal morphology raises the interesting point of whether there are daily rhythm or gender differences in S100beta level in the brain. To answer this question, the S100beta expression in adult female and male rats, as well as in adult female CD-21 and S100beta -/- female mice, were investigated. Scintillation counting and morphometric analysis of the immunoreactivity of S100beta, showed rhythmic daily expression. The female and male rats showed opposite cycles. Females presented the highest value at the beginning of the rest phase (5:00 h), while in males the maximum value appeared in the beginning of the motor activity period (21:00 h). These results confirm previous S100beta evaluations in human serum and cerebrospinal fluid reporting the protein's function as a biomarker for brain damage (Gazzolo et al. in Clin Chem 49:967-970, 2003; Clin Chim Acta 330:131-133, 2003; Pediatr Res 58:1170-1174, 2005), similar behavior was also observed for GFAP in relation to Alzheimer Disease (Fukuyama et al. in Eur Neurol 46:35-38, 2001). The data should be taken into account when considering S100beta as a biomarker of health condition. In addition, the results raise questions on which structure or condition imposes these rhythms as well as on the physiological meaning of the observed gender differences.


Subject(s)
Aging/metabolism , Nerve Growth Factors/biosynthesis , S100 Proteins/biosynthesis , Animals , Circadian Rhythm/physiology , Female , Hippocampus/cytology , Hippocampus/growth & development , Hippocampus/metabolism , Humans , Immunoassay , Immunohistochemistry , Male , Mice , Mice, Knockout , Motor Activity/physiology , Nerve Growth Factors/genetics , Prosencephalon/metabolism , Rats , S100 Calcium Binding Protein beta Subunit , S100 Proteins/genetics , Sex Characteristics
6.
Astrobiology ; 9(1): 1-22, 2009.
Article in English | MEDLINE | ID: mdl-19203238

ABSTRACT

The discovery of extrasolar planets is one of the greatest achievements of modern astronomy. The detection of planets that vary widely in mass demonstrates that extrasolar planets of low mass exist. In this paper, we describe a mission, called Darwin, whose primary goal is the search for, and characterization of, terrestrial extrasolar planets and the search for life. Accomplishing the mission objectives will require collaborative science across disciplines, including astrophysics, planetary sciences, chemistry, and microbiology. Darwin is designed to detect rocky planets similar to Earth and perform spectroscopic analysis at mid-infrared wavelengths (6-20 mum), where an advantageous contrast ratio between star and planet occurs. The baseline mission is projected to last 5 years and consists of approximately 200 individual target stars. Among these, 25-50 planetary systems can be studied spectroscopically, which will include the search for gases such as CO(2), H(2)O, CH(4), and O(3). Many of the key technologies required for the construction of Darwin have already been demonstrated, and the remainder are estimated to be mature in the near future. Darwin is a mission that will ignite intense interest in both the research community and the wider public.


Subject(s)
Exobiology/methods , Extraterrestrial Environment , Origin of Life , Planets , Space Flight , Astronomy , Bayes Theorem , Image Processing, Computer-Assisted , Spacecraft , Spectrophotometry, Infrared , Stars, Celestial
7.
QJM ; 101(2): 99-109, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18194975

ABSTRACT

AIM: To identify case-mix variables measured shortly after admission to be included in a patient classification system (ACMEplus) that best explains hospital outcome for older people in different health care systems. DESIGN: Observational prospective cohort study collecting patient factors (sociodemographics, functional, mental, clinical, administrative and perceived health) at different time assessments. METHODS: Multicentre study involving eight hospitals in six European countries (United Kingdom, Spain, Italy, Finland, Greece and Poland). It included consecutive patients aged 65 years or older admitted to hospital for acute medical problems. MAIN OUTCOME MEASURES: discharge status, hospital readmission, mortality and length of stay. RESULTS: Of the 1667 included patients (mean age = 78.1 years; male gender = 43.5%) two-third had at least one 'Geriatric Giant' (immobility, confusion, incontinence or falls) on admission or shortly after. The most frequently affected system was cardiovascular (29.2%) and 31% of patients declared poor or very poor health. Mean length of stay was 17.9 days, 79% of patients were discharged to their usual residence; in-hospital and 1-month follow up mortality were 7.4% and 11.6%, respectively. Physical function explained the highest variation (between 8% and 21%), followed by cognitive status and number of Geriatric Giants, for almost all outcomes except readmission. CONCLUSION: Factors other than diagnosis (physical function, cognition and presenting problems) are important in predicting key outcomes of acute hospital care for older people and are consistent across countries. Their inclusion in a standardized system of measurement may be a way of improving quality and equity of medical care in older people.


Subject(s)
Diagnosis-Related Groups , Health Status , Hospitalization , Outcome Assessment, Health Care , Acute Disease , Aged , Aged, 80 and over , Diagnosis-Related Groups/classification , Diagnosis-Related Groups/statistics & numerical data , Europe , Female , Hospitalization/statistics & numerical data , Humans , International Cooperation , Male , Outcome Assessment, Health Care/classification , Outcome Assessment, Health Care/statistics & numerical data , Program Development , Prospective Studies , Socioeconomic Factors
8.
Appl Opt ; 47(31): 5728-35, 2008 Nov 01.
Article in English | MEDLINE | ID: mdl-19122712

ABSTRACT

We demonstrate the modal filtering properties of newly developed single mode silver halide fibers for use at midinfrared wavelengths, centered at 10.5 microm. The goal was to achieve a suppression of nonfundamental modes greater than a factor of 300 to enable the detection and characterization of Earthlike exoplanets with a space-based nulling interferometer. Fiber segments of 4.5 cm, 10.5 cm, 15 cm, and 20 cm lengths were tested. We find that the performance of the fiber was limited not by the modal filtering properties of the core but by the unsuppressed cladding modes present at the output of the fiber. In 10.5 cm and longer sections, this effect can be alleviated by properly aperturing the output. Exclusive of coupling losses, the fiber segments of 10.5-20 cm length can provide power suppression of undesirable components of the input field by a factor of 15,000 at least. The demonstrated performance thus far surpasses our requirements, such that even very short sections of fiber provide adequate modal filtering for exoplanet characterization.

9.
Evol Appl ; 1(2): 252-70, 2008 May.
Article in English | MEDLINE | ID: mdl-25567630

ABSTRACT

Salmon life histories are finely tuned to local environmental conditions, which are intimately linked to climate. We summarize the likely impacts of climate change on the physical environment of salmon in the Pacific Northwest and discuss the potential evolutionary consequences of these changes, with particular reference to Columbia River Basin spring/summer Chinook (Oncorhynchus tshawytscha) and sockeye (Oncorhynchus nerka) salmon. We discuss the possible evolutionary responses in migration and spawning date egg and juvenile growth and development rates, thermal tolerance, and disease resistance. We know little about ocean migration pathways, so cannot confidently suggest the potential changes in this life stage. Climate change might produce conflicting selection pressures in different life stages, which will interact with plastic (i.e. nongenetic) changes in various ways. To clarify these interactions, we present a conceptual model of how changing environmental conditions shift phenotypic optima and, through plastic responses, phenotype distributions, affecting the force of selection. Our predictions are tentative because we lack data on the strength of selection, heritability, and ecological and genetic linkages among many of the traits discussed here. Despite the challenges involved in experimental manipulation of species with complex life histories, such research is essential for full appreciation of the biological effects of climate change.

10.
Contemp Top Lab Anim Sci ; 44(3): 18-21, 2005 May.
Article in English | MEDLINE | ID: mdl-15934718

ABSTRACT

In this study, we fed a standard NIH-31 diet fortified with vitamin E to C57BL/6 mice and strains of mice with a C57BL/6 background that had spontaneously developed ulcerative dermatitis (UD). In addition to the therapeutic response to increased levels of vitamin E, we also defined the occurrence of UD within our facility in terms of age, sex, coat color, and lesion location on the body. Mice with spontaneous UD were fed a vitamin E-fortified diet (3000 IU/kg) for a period of 8 weeks and entered the study without regard to vendor source, age, sex, coat color, or the site or number of UD lesions. We found that lesions occurred most commonly on the dorsal cervical and scapular regions and spared the ventral abdomen and thorax. No sex or coat color predilection was noted for the development of UD, however males were older than females at the time of lesion development. Of 71 mice, 32 (45%) had complete lesion re-epithelialization with hair regrowth. Complete lesion repair was not influenced by sex, age, or coat color. The average time to complete lesion repair ranged from 2 to 5 weeks, and there was no correlation with sex or coat color. The positive response to vitamin E suggests that protection from oxidative injury may play a role in the resolution of UD lesions and offers veterinarians and investigators a new treatment option with ease of compliance.


Subject(s)
Dermatitis, Atopic/veterinary , Diet , Food, Fortified , Rodent Diseases/drug therapy , Skin Ulcer/veterinary , Veterinary Medicine/methods , Vitamin E/administration & dosage , Animals , Dermatitis, Atopic/drug therapy , Dermatitis, Atopic/pathology , Female , Male , Mice , Mice, Inbred C57BL , Rodent Diseases/pathology , Skin Ulcer/drug therapy , Skin Ulcer/pathology , Treatment Outcome
12.
Int J Syst Evol Microbiol ; 55(Pt 2): 643-647, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15774637

ABSTRACT

Five strains of an unusual Gram-negative, catalase-positive, oxidase-positive, coccobacillus-shaped bacterium isolated from the lungs and heart of pigs with pneumonia and pericarditis were characterized by phenotypic and molecular genetic methods. On the basis of cellular morphology and biochemical criteria, the isolates were tentatively assigned to the family Neisseriaceae, although they did not appear to correspond to any recognized genus or species. Comparative 16S rRNA gene sequencing showed that the five unidentified strains were phylogenetically highly related to each other and represent a hitherto unknown subline within the family Neisseriaceae. On the basis of both phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from pigs be classified as a novel genus and species within the family Neisseriaceae, for which the name Uruburuella suis gen. nov., sp. nov. is proposed. The type strain of U. suis is 1258/02(T) (=CCUG 47806(T)=CECT 5685(T)).


Subject(s)
Neisseriaceae/classification , Neisseriaceae/genetics , Pericarditis/veterinary , Phylogeny , Pneumonia, Bacterial/veterinary , Swine Diseases/microbiology , Animals , Bacterial Typing Techniques , DNA, Bacterial/analysis , DNA, Ribosomal/analysis , Genes, rRNA , Heart/microbiology , Lung/microbiology , Molecular Sequence Data , Neisseriaceae/isolation & purification , Neisseriaceae/physiology , Neisseriaceae Infections/microbiology , Neisseriaceae Infections/veterinary , Pericarditis/microbiology , Phenotype , Pneumonia, Bacterial/microbiology , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Swine
13.
J Hosp Infect ; 52(4): 250-8, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12473468

ABSTRACT

During a nine-day period, five patients in a 14-bed intensive care unit (ICU) were shown to have seroconverted with a four-fold or greater rise in serum antibody titre to Legionella longbeachae serogroup 1. A further two patients were observed to have high titres consistent with previous exposure but earlier serum samples were not available for comparison. No patients had antibody responses to Legionella pneumophila serogroups 1 and 2. L. longbeachae was not cultured from respiratory secretions from patients or from the environment within the unit. Legionella anisa was recovered from one cooling tower on the ninth floor of the tower block. The ICU is located on the first floor of the same tower and receives external air from two vents, one on the eastern and the other on the western aspect. All patients with serological evidence of L. longbeachae infection were concomitantly infected with multiresistant Staphylococcus aureus, and were located in bays on the eastern side of the unit. A large pigeon nest was discovered within 1-2 m of the eastern vent. Following removal of the birds' nest, no further cases were seen on routine screening of all patients within the unit over the next eight weeks. Alternatively, seroconversion may have been related to demolition of the adjacent nine-storey nurses home. This was begun one month before the first case was diagnosed and was completed four months later. The periodic northerly winds could have carried legionellae from the demolition site directly over the block housing the ICU and may have concentrated them near the eastern air vent. All patients had pneumonia, which was probably multifactorial in origin. There is some uncertainty whether the serological responses seen were an epiphenomenon or were truly indicative of infection with L. longbeachae.


Subject(s)
Air Conditioning , Cross Infection/etiology , Disease Outbreaks/statistics & numerical data , Intensive Care Units , Legionellosis/etiology , Pneumonia, Bacterial/etiology , Water Microbiology , Aged , Animals , Antibodies, Bacterial/blood , Columbidae/microbiology , Cross Infection/diagnosis , Cross Infection/epidemiology , Cross Infection/prevention & control , Disease Outbreaks/prevention & control , Environmental Monitoring/methods , Epidemiological Monitoring , Female , Hospital Design and Construction , Humans , Infection Control/methods , Interior Design and Furnishings , Legionellosis/diagnosis , Legionellosis/epidemiology , Legionellosis/prevention & control , Male , Methicillin Resistance , Middle Aged , Pneumonia, Bacterial/diagnosis , Pneumonia, Bacterial/epidemiology , Pneumonia, Bacterial/prevention & control , Risk Factors , South Australia/epidemiology , Staphylococcal Infections/etiology , Staphylococcus aureus
14.
Int J Syst Evol Microbiol ; 52(Pt 2): 665-9, 2002 Mar.
Article in English | MEDLINE | ID: mdl-11931181

ABSTRACT

Biochemical, molecular chemical and molecular genetic studies were performed on an unknown gram-positive, catalase-negative, coccus-shaped organism isolated from the intestine of a cow affected with catarrhal enteritis. The organism was tentatively identified as a streptococcal species based on results of cellular morphological and biochemical tests. 16S rRNA gene sequencing studies confirmed its provisional identification as a member of the genus Streptococcus, but the organism did not correspond to any recognized species of this genus. The nearest phylogenetic relatives of the unknown coccus from a calf were Streptococcus acidominimus and Streptococcus suis. The unknown bacterium, however, was distinguished from these species and other animal streptococci by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on both phenotypic and phylogenetic findings, it is proposed that the unknown bacterium be classified as a novel species of the genus Streptococcus, Streptococcus entericus sp. nov. The type strain is CECT 5353T (= CCUG 44616T).


Subject(s)
Cattle Diseases/microbiology , Diarrhea/veterinary , Streptococcal Infections/veterinary , Streptococcus/classification , Animals , Animals, Suckling , Cattle , Diarrhea/microbiology , Genes, rRNA , Molecular Sequence Data , Phylogeny , RNA, Bacterial/chemistry , RNA, Ribosomal, 16S/chemistry , Species Specificity , Streptococcal Infections/microbiology , Streptococcus/genetics , Streptococcus/isolation & purification
16.
Int J Syst Evol Microbiol ; 51(Pt 3): 853-855, 2001 May.
Article in English | MEDLINE | ID: mdl-11411706

ABSTRACT

Three strains of a previously undescribed Actinomyces-like bacterium were isolated from human clinical specimens. Phenotypic studies indicated that the strains were members of the genus Actinomyces and were presumptively identified as Actinomyces turicensis. Comparative 16S rRNA gene sequencing studies showed that although the bacterium is phylogenetically closely related to Actinomyces turicensis, it nevertheless constitutes a new sub-line within the genus Actinomyces. Based on phenotypic and molecular chemical and molecular genetic evidence, it is proposed that the unknown Actinomyces-like bacterium from human clinical specimens be classified as Actinomyces funkei sp. nov. The type strain of Actinomyces funkei is CCUG 42773T (= CIP 106713T).


Subject(s)
Actinomycetales/classification , Phylogeny , Actinomycetales/genetics , Actinomycetales/isolation & purification , Adult , Cefuroxime/therapeutic use , DNA, Bacterial/genetics , DNA, Ribosomal/genetics , Drug Therapy, Combination/therapeutic use , Endocarditis, Bacterial/complications , Endocarditis, Bacterial/drug therapy , Female , Humans , Molecular Sequence Data , Phenotype , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Staphylococcal Infections/complications , Staphylococcal Infections/drug therapy , Substance Abuse, Intravenous/complications
17.
Int J Syst Evol Microbiol ; 51(Pt 3): 967-970, 2001 May.
Article in English | MEDLINE | ID: mdl-11411722

ABSTRACT

A Gram-positive, non-spore-forming, catalase-negative, facultatively anaerobic coccibacillus to rod-shaped bacterium isolated from a parrot was characterized using phenotypic and molecular taxonomic methods. The unknown bacterium phenotypically resembled lactobacilli and comparative 16S rRNA gene sequencing demonstrated that the organism represents a distinct subline within the Lactobacillus delbrueckii rRNA cluster of the genus. 16S rRNA sequence divergence values of > 6% with recognized Lactobacillus species clearly demonstrated the phylogenetic separateness of the parrot bacterium. On the basis of phylogenetic evidence and the phenotypic distinctiveness of the unknown bacterium, a new species, Lactobacillus psittaci sp. nov., is proposed. The type strain of Lactobacillus psittaci is CCUG 42378T (= CIP 106492T).


Subject(s)
DNA, Ribosomal/genetics , Lactobacillus/classification , Lactobacillus/genetics , Parrots/microbiology , Phylogeny , Psittaciformes/microbiology , RNA, Ribosomal, 16S/genetics , Animals , Genetic Variation , Lactobacillus/isolation & purification , Male , Molecular Sequence Data , Phenotype
18.
Int J Syst Evol Microbiol ; 51(Pt 2): 425-431, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11324591

ABSTRACT

A spore-forming, halophilic bacterium was isolated from surface sediment located on the beach of Palaeochori Bay near to a shallow water hydrothermal vent area, Milos, Greece. The bacterium, designated SH 714T, consisted of motile, strictly aerobic rods which contained an Orn-D-Glu type murein and a G+C content of 35 mol%. Thin sections showed a cell wall typical for Gram-positive bacteria; the peptidoglycan layer, however, was very thin. The Gram-reaction of the organism was negative. Comparative 16S rRNA gene sequencing demonstrated that the isolate represents a new line of descent within the spore-forming rods branching at the periphery of the rRNA group 1 Bacillus (Bacillus sensu stricto). The nearest phylogenetic neighbours of the unknown bacterium were Bacillus haloalkaliphilus, Marinococcus albus and Halobacillus species. Based on phylogenetic and phenotypic evidence it is proposed that the unknown bacterium be classified as Filobacillus milensis gen. nov., sp. nov. The type strain is SH 714T (= DSM 13259T = ATCC 700960T).


Subject(s)
Bacillus/classification , Peptidoglycan/chemistry , Water Microbiology , Bacillus/chemistry , Bacillus/genetics , Bacillus/ultrastructure , Bacteria, Aerobic , DNA, Ribosomal , Geologic Sediments , Glutamic Acid/analysis , Hot Temperature , Molecular Sequence Data , Ornithine/analysis , RNA, Ribosomal, 16S , Seawater , Terminology as Topic
19.
Int J Syst Evol Microbiol ; 51(Pt 2): 475-479, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11321093

ABSTRACT

Phenotypic and phylogenetic studies were performed on an unknown, Gram-positive, catalase-negative coccus isolated from human blood. Comparative 16S rRNA gene sequencing demonstrated that the organism represents a new subline within the genus Aerococcus. The unknown bacterium was readily distinguished from the three currently recognized Aerococcus species, Aerococcus christensenii, Aerococcus urinae and Aerococcus viridans, by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that this unknown bacterium from blood be classified as Aerococcus sanguicola sp. nov. The type strain of Aerococcus sanguicola is CCUG 43001T (= CIP 106533T).


Subject(s)
Blood/microbiology , Streptococcaceae/classification , DNA, Ribosomal/genetics , Molecular Sequence Data , RNA, Ribosomal, 16S/genetics , Streptococcaceae/genetics , Streptococcaceae/isolation & purification
20.
Int J Syst Evol Microbiol ; 51(Pt 2): 683-686, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11321115

ABSTRACT

Phenotypic and phylogenetic studies were performed on an unknown Gram-positive catalase-negative coccus isolated from human urine. Comparative 16S rRNA gene sequencing demonstrated that the organism represents a new subline within the genus Aerococcus. The unknown bacterium was readily distinguished from the three currently recognized Aerococcus species, Aerococcus christensenii, Aerococcus urinae and Aerococcus viridans, by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium from urine be classified as Aerococcus urinaehominis sp. nov. The type strain of Aerococcus urinaehominis is CCUG 42038bT (= CIP 106675T).


Subject(s)
Bacteriuria/microbiology , Streptococcaceae/classification , Bacterial Typing Techniques , Genes, rRNA/genetics , Humans , Molecular Sequence Data , Phylogeny , RNA, Ribosomal, 16S/genetics , Streptococcaceae/isolation & purification , Terminology as Topic
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