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Biotechniques ; 13(6): 892-4, 897, 1992 Dec.
Article in English | MEDLINE | ID: mdl-1476741

ABSTRACT

Some primers frequently used in the double-stranded dideoxy DNA sequencing technique with radioactive markers are not suited for fluorescent detection. In fact oligonucleotides have a different annealing efficiency related to their base sequence, and this is reflected in nonequivalent results, particularly in fluorescent automated DNA sequencing. We present a method for the evaluation of primer performance in automated DNA sequencers and show its application to the search for a better set of primers for pBluescript vector.


Subject(s)
Base Sequence , DNA/genetics , Genetic Techniques , DNA Probes , Evaluation Studies as Topic , Molecular Probe Techniques , Molecular Sequence Data , Thermodynamics
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