ABSTRACT
This study aimed to compare the in vivo effectiveness between curcumin-oligochitosan nanoplexes (CUR-OCH nanoplexes) and oligochitosan-coated curcumin-encapsulated liposomes (OCH-Lip-CUR) with respect to wound healing and scar treatment. Firstly, CUR-OCH nanoplexes was prepared by drug-polysaccharide complexation method and OCH-Lip-CUR was prepared by a combining method of lipid-film hydration and sonication. Their in vitro cytotoxicity and in vivo wound healing and scar treatment effectiveness were evaluated using 3T3 cells and mice Mus musculus var. Albino, respectively. The resutls indicated that both of them were in nanosize with a moderate PDI (less than 0.3), and exhibited negligible cytotoxicity at low CUR concentration (0.01 mg/mL). Moreover, their application onto wounds resulted in faster healing and higher scar treatment effectiveness than control samples. Interestingly, OCH-Lip-CUR exhibited higher in vivo effectiveness than CUR-OCH nanoplexes. However, based on their own advantages, both of them were good candidates for a commercial formulation for wound healing and scar treatment.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/administration & dosage , Cicatrix/drug therapy , Curcumin/administration & dosage , Wound Healing/drug effects , 3T3 Cells , Animals , Anti-Inflammatory Agents, Non-Steroidal/chemistry , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Chitin/analogs & derivatives , Chitin/chemistry , Chitosan , Curcumin/analogs & derivatives , Curcumin/therapeutic use , Drug Liberation , Liposomes/chemistry , Male , Mice , Nanoconjugates/chemistry , OligosaccharidesABSTRACT
A study on nurses and delivers were carried out at 14 hospitals, Medical Centers belong to Ha Noi Department of Health and 14 National Hospitals, Hospital belong to some ministries that located in Ha Noi. The results showed that: in these hospitals, the number of nurses and delivers don’t meet the requirements of the Ministry of Health‘s rule. The index of doctor/nurse and deliver: 1/1.49; Professional qualification: most of them are secondary school (nurses: 92%, delivers: 90.48%), low rate in nurse and deliver whom hold university or junior college bachelor: in 3 facilities, a few nurses who are university bachelor (0.7%); in 7 facilities, there are nurses who graduated from junior colleges (1.9%). There is not any hospital among 14 hospitals which the number of doctor/nurse get the standard as the ministry of health rule.( the highest rate in Viet Duc hospital:1/1,9, Pediatric hospital:1/1,8. Most of them graduated from secondary school (71.7% to 94.4%), 1.6% nurses are university bachelor and 6.9% nurse are junior college bachelor, 9 hospitals have nurse with elementary qualification.
