Inhibition of Pancreatic Lipase by Flavonoid Derivatives: In Vitro and In Silico Investigations.

Obesity, characterized by excessive adipose tissue accumulation, has emerged as a crucial determinant for a wide range of chronic medical conditions. The identification of effective interventions for obesity is of utmost importance. Widely researched antiobesity agents focus on pancreatic lipase, a significant therapeutic target. This study presented the evaluation of ten flavonoid compounds in terms of their inhibitory activities against pancreatic lipase, utilizing both in vitro and in silico approaches. The results indicated that all tested compounds demonstrated modest and weaker inhibitory activities compared to the reference compound, orlistat. Among the compounds investigated, F01 exhibited the highest potency, with an IC50 value of 17.68 ± 1.43 µM. The enzymatic inhibition kinetic analysis revealed that F01 operated through a competitive inhibition mechanism with a determined Ki of 7.16 µM. This value suggested a moderate binding affinity for the pancreatic lipase enzyme. Furthermore, the associated Vmax value was quantified at 0.03272 ΔA·min-1. In silico studies revealed that F01 displayed a binding mode similar to that of orlistat, despite lacking an active functional group capable of forming a covalent bond with Ser152 of the catalytic triad. However, F01 formed a hydrogen bond with this crucial amino acid. Furthermore, F01 interacted with other significant residues at the enzyme's active site, particularly those within the lid domain. Based on these findings, F01 demonstrates substantial potential as a candidate for further investigations.

Characteristics, causes and sequel of pleural thickening in pleural effusion in children

145 children with pleural effusion were investigated at HCM Pediatric Hospital from Jan 1999 to May 2002. Results found that 53,1% of cases occurred in under five years old children, among them 62,8% were admitted in Hospital > 7 days after onset of the disease with the symptoms of fever, tachypnoe, dyspnoe, cough, lung pain. The effusion was commonly occurred in the right side . Pleural effusion S. Aureus was usually occurred 54% off cases were pleural thickening and adhesive. The mortality acconnted for 2,8% with the cause of sepsis and respiratory distress.

Contributing in the research of hematuria in children

71 patients with macroscopic hematuria in 923 patients were treated at the Nephrology Department in Nhi Dong I hospital from November 01 to June 30, 2002, accounted for 7.69%. The mean age was 7.73  3.36 years old. The highest level at age group 4-10. Boys were more affted than girls. The symptoms caused by glomerulo-hematuria were anorexia, edema, hypertension, respiratory signs, urine with dark brown, pink color, dysmorphic red blood cells. Urinary signs, urine with bright red color or blood clots, non-dysmorphic red cells were the symptoms which indicating non-glomerulo hematuria. The most common causes were glomerulo-nephritis accounted for 69%, in which post-streptococcal acute glomerulonephritis was 38.1%. In addition, the recurrent hematuria was 12.7% in which the IgA nephropathy was the most 55.6%. The others were hypercalciuria, nephrotic syndrome, lupus, Henoch-Schonlein

The features of infection of Helicobacter pylori in Hospital I of Pediatrics

In 47 studied cases of Helicobacter pylori infection of children, the age group from 7-10 was the most common. Main clinical symptoms were chronic abdominal pain without typical signs. The infection was usually accompanied with chronic inflammation of gastric mucous membrane, especially in antrum. Therefore further study must be conducted in large population to monitor the progression of lesions and to determine their relations with gastric cancer in adult

Characteristics of thrombocytopenia hemorrhage in infants at Pediatric Hospital No2

Study on having glomerule <100.000/mm3 of 114 children at the 2 to 12 years of age, who treated at inner synthetic deparment 4 of Infant Hospital 2 from 5/2002 to 5/2003. Result: The incidence haemorrhage reduce glimerule rate in infant is 34% (48/144 cases) all of suffer the disease case enter hospital. Boy per girl rate is 1,527. The disease commons in lower 6 months children. The acute initial development relative to vaccinate of infect super bacteria. Clinical symptom is severe: hypodermic haemorrhage in the form of spot in skill and the upper jaw mucous membrane but rarely intracerebral haemorrhage. Para clinical symptoms is glomerule reduce to < 20.000/mm3 but recover quickly