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1.
Analyst ; 140(13): 4521-9, 2015 Jul 07.
Article in English | MEDLINE | ID: mdl-25988771

ABSTRACT

Metabolic profiling is increasingly being used for understanding biological processes but there is no single analytical technique that provides a complete quantitative or qualitative profiling of the metabolome. Data fusion (i.e. joint analysis of data from multiple sources) has the potential to circumvent this issue facilitating knowledge discovery and reliable biomarker identification. Another field of application of data fusion is the simultaneous analysis of metabolomic changes through several biofluids or tissues. However, metabolomics typically deals with large datasets, with hundreds to thousands of variables and the identification of shared and individual factors or structures across multiple sources is challenging due to the high variable to sample ratios and differences in intensity and noise range. In this work we apply a recent method, Joint and Individual Variation Explained (JIVE), for the integrated unsupervised analysis of metabolomic profiles from multiple data sources. This method separates the shared patterns among data sources (i.e. the joint structure) from the individual structure of each data source that is unrelated to the joint structure. Two examples are described to show the applicability of JIVE for the simultaneous analysis of multi-source data using: (i) plasma samples subjected to different analytical techniques, sample treatment and measurement conditions; and (ii) plasma and urine samples subjected to liquid chromatography-mass spectrometry measured using two ionization conditions.


Subject(s)
Metabolomics/methods , Statistics as Topic/methods , Blood Chemical Analysis , Humans , Software , Urinalysis
2.
Anal Chim Acta ; 752: 11-29, 2012 Nov 08.
Article in English | MEDLINE | ID: mdl-23101648

ABSTRACT

Organic UV filters are chemical compounds added to cosmetic sunscreen products in order to protect users from UV solar radiation. The need of broad-spectrum protection to avoid the deleterious effects of solar radiation has triggered a trend in the cosmetic market of including these compounds not only in those exclusively designed for sun protection but also in all types of cosmetic products. Different studies have shown that organic UV filters can be absorbed through the skin after topical application, further metabolized in the body and eventually excreted or bioaccumulated. These percutaneous absorption processes may result in various adverse health effects, such as genotoxicity caused by the generation of free radicals, which can even lead to mutagenic or carcinogenic effects, and estrogenicity, which is associated with the endocrine disruption activity caused by some of these compounds. Due to the absence of official monitoring protocols, there is a demand for analytical methods that enable the determination of UV filters in biological fluids and tissues in order to retrieve more information regarding their behavior in the human body and thus encourage the development of safer cosmetic formulations. In view of this demand, there has recently been a noticeable increase in the development of sensitive and selective analytical methods for the determination of UV filters and their metabolites in biological fluids (i.e., urine, plasma, breast milk and semen) and tissues. The complexity of the biological matrix and the low concentration levels of these compounds inevitably impose sample treatment processes that afford both sample clean-up to remove potentially interfering matrix components as well as the enrichment of analytes in order to achieve their determination at very low concentration levels. The aim of this review is to provide a comprehensive overview of the recent developments in the determination of UV filters in biological fluids and tissues, with special emphasis on the elucidation of new metabolites, sample preparation and analytical techniques as well as occurrence levels.


Subject(s)
Body Fluids/chemistry , Organic Chemicals/analysis , Animals , Body Fluids/metabolism , Humans , Organic Chemicals/metabolism , Organic Chemicals/pharmacokinetics , Tissue Distribution
3.
Anal Bioanal Chem ; 401(3): 1003-11, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21637928

ABSTRACT

The proven endocrine disruption nature of the sunscreen ingredient 2-ethylhexyl 4-(N,N-dimethylamino)benzoate (EDP) calls for research to understand its distribution and bioaccumulation in the human body. A sensitive analytical method to determine EDP and its metabolites in human semen based on online SPE-LC-MS/MS is described. The method has been fully validated and a standard addition calibration has been used for quantification to correct the observed matrix effects. The on-column detection limits of the analytes are between 0.2 and 0.6 ng, depending on the analyte and the sample. The repeatability of the method, expressed as relative standard deviation, was in the range 4.6-9.4%. The method was satisfactorily applied to semen samples from male volunteers who were subjected to single and repeated whole-body applications of an EDP-containing sunscreen product. EDP metabolites were found at different concentrations in semen samples from the repeated application study, thus showing evidences of bioaccumulation in humans.


Subject(s)
4-Aminobenzoic Acid/metabolism , Chromatography, Liquid , Semen/chemistry , Sunscreening Agents/metabolism , Tandem Mass Spectrometry , 4-Aminobenzoic Acid/analysis , Humans , Male , Molecular Structure , Reproducibility of Results , Semen/metabolism , Solid Phase Extraction , Sunscreening Agents/analysis
4.
J Chromatogr A ; 1218(20): 3013-21, 2011 May 20.
Article in English | MEDLINE | ID: mdl-21481400

ABSTRACT

The in vivo metabolism of the xenobiotic agent 2-ethylhexyl 4-(N,N-dimethylamino)benzoate (EDP), a UV filter commonly used in sunscreen cosmetic products, was studied by targeting metabolomics analysis in human urine. The metabolomic study involved the use of urine from male and female volunteers before and after application of an EDP-containing sunscreen cosmetic. The metabolism of EDP in urine was studied by using the triple quadrupole detector in a combination of Precursor Ion Scanning and Neutral Loss Scanning modes, with and without enzymatic hydrolysis. Detected metabolites were subsequently confirmed as glucuronide conjugates of 4-(N,N-dimethylamino)benzoic acid and 4-(N-methylamino)benzoic acid by liquid chromatography-time-of-flight/mass spectrometry (LC-TOF/MS) in the accurate mass mode. In this way, the existence of phase II metabolism in the detoxification of EDP by effects of the lipophilic character of this sunscreen agent was confirmed. Hence, to study the in vivo metabolism of EDP, a fully automated method using a solid-phase extraction (SPE) workstation connected on-line to a liquid chromatograph and a triple quadrupole mass analyzer (LC-MS/MS) was developed. The ensuing hyphenated method is very simple and requires minimal human intervention. Following thorough optimization of the SPE and LC-MS/MS conditions, the analytical procedure was validated and standard addition calibration used for the quantitative correction of matrix effects. The proposed method was applied to determine the phase I metabolites of EDP in urine samples and afforded limits of detection from 0.1 to 1.1 ng and accuracy of 91-107% with relative standard deviations in the range 1.5-8.7% (sample volume: 100 µL). Based on the results of in vivo percutaneous absorption of a single application of the sunscreen, about 0.5% of the amount of the applied EDP is excreted in urine.


Subject(s)
Chromatography, Liquid/methods , Metabolomics/methods , Solid Phase Extraction/methods , Sunscreening Agents/analysis , para-Aminobenzoates , 4-Aminobenzoic Acid/metabolism , 4-Aminobenzoic Acid/urine , Drug Stability , Female , Glucuronidase/chemistry , Glucuronidase/metabolism , Humans , Hydrolysis , Male , Metabolome , Skin Absorption , Sonication , Sulfatases/chemistry , Sulfatases/metabolism , Sunscreening Agents/metabolism , Tandem Mass Spectrometry/methods
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