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1.
Neuromuscul Disord ; 31(8): 726-735, 2021 08.
Article in English | MEDLINE | ID: mdl-34304969

ABSTRACT

The tolerance of exercise and its effects on quality of life in myasthenia gravis are not currently backed up by strong evidence. The aim of this study was to determine whether exercise as an adjunct therapy is well tolerated and can improve health-related quality of life (HRQoL) in stabilized, generalized autoimmune myasthenia gravis (gMG). We conducted a parallel-group, multi-center prospective RCT using computer-generated block randomization. Adults with stabilized, gMG, and no contra-indication to exercise, were eligible. Participants received usual care alone or usual care and exercise. The exercise intervention consisted of 3-weekly 40 min sessions of an unsupervised, moderate-intensity home rowing program over 3 months. The primary endpoint was the change in HRQoL from randomization to post-intervention. Assessor-blinded secondary endpoints were exercise tolerance and effects on clinical, psychological and immunological status. Of 138 patients screened between October 2014 and July 2017, 45 were randomly assigned to exercise (n = 23) or usual care (n = 20). Although exercise was well tolerated, the intention-to-treat analysis revealed no evidence of improved HRQoL compared to usual care (MGQOL-15-F; mean adjusted between-groups difference of -0.8 points, 95%CI -5.4 to 3.7). Two patients hospitalized for MG exacerbation were from the usual care group.


Subject(s)
Exercise Therapy/methods , Myasthenia Gravis/therapy , Adult , Aged , Exercise , Exercise Tolerance , Female , Humans , Male , Middle Aged , Prospective Studies , Quality of Life
2.
Rev Med Interne ; 35(7): 413-20, 2014 Jul.
Article in French | MEDLINE | ID: mdl-24156976

ABSTRACT

Myasthenia gravis is characterized by muscle weakness and abnormal fatigability. It is an autoimmune disease caused by the presence of antibodies against components of the muscle membrane localized at the neuromuscular junction. In most cases, the autoantibodies are directed against the acetylcholine receptor (AChR). Recently, other targets have been described, such as muscle-specific kinase protein (MuSK) or lipoprotein related protein 4 (LRP4). The origin of the autoimmune response is not known, but thymic abnormalities and defects in immune regulation certainly play a major role in patients with anti-AChR antibodies. Genetic predisposition probably influences the occurrence of the disease. Sex hormones seem to play a role in the early form of the disease. Muscle weakness is fluctuating and worsens with exercise. Myasthenia gravis could be classified according to the location of the affected muscles (ocular versus generalized), the age of onset of symptoms, thymic abnormalities and profile of autoantibodies. These criteria are used to optimize the management and treatment of patients. In this review, we analyze the latest concepts of the pathophysiology of myasthenia gravis according to the different subgroups of the disease, including a description of the role of immunological, genetic and environmental factors. The potential viral hypothesis of this disease is discussed. Finally, we also discuss the biological assays available to validate the diagnosis.


Subject(s)
Autoantibodies/blood , Myasthenia Gravis/physiopathology , Humans , LDL-Receptor Related Proteins/immunology , Neuromuscular Junction/immunology , Receptor Protein-Tyrosine Kinases/immunology , Receptors, Cholinergic/immunology , Thymus Gland/physiopathology
3.
Rev Neurol (Paris) ; 169(8-9): 640-9, 2013.
Article in English | MEDLINE | ID: mdl-24008049

ABSTRACT

In autoimmune Myasthenia Gravis (MG), a neuromuscular disease generally mediated by autoantibodies against the acetylcholine receptor (AChR), the muscle is the target organ of the autoimmune attack, while the thymus seems to be the primary production site of the autoantibodies. In the majority of patients with anti-AChR antibodies, it is characterized by the presence of germinal centers, which contain B cells that produce anti-AChR antibodies. In this review, we summarize recent results regarding neoangiogenic processes, cell infiltration and modified chemokine expression in the MG thymus, which are typical features of secondary lymphoid organs. The structural and functional changes in the MG thymus therefore allow us to declare it to be an archetype for tertiary lymphoid neogenesis providing optimal settings for the interaction between lymphocytes and antigen presenting cells in order to elicit an immune response. We further discuss factors that may have a key role in the transformation of the MG thymus into a tertiary lymphoid organ, such as IFN type I and dsRNA signaling. These factors could also be of importance in other autoimmune diseases, especially those characterized by tertiary lymphoid neogenesis.


Subject(s)
Lymphoid Tissue/immunology , Myasthenia Gravis/physiopathology , Thymus Gland/physiology , Animals , Autoantibodies/metabolism , Humans , Lymphoid Tissue/growth & development , Myasthenia Gravis/pathology , Thymus Gland/pathology , Thymus Hyperplasia/immunology , Thymus Hyperplasia/metabolism , Virus Diseases/complications , Virus Diseases/immunology
4.
Cell Death Differ ; 12(5): 463-72, 2005 May.
Article in English | MEDLINE | ID: mdl-15775997

ABSTRACT

Thymic myoid cells correspond to a muscle-like cell population present in the thymic medulla. They are well conserved throughout species evolution, but their biological role is not known. We demonstrated that myoid cells protected thymocytes from apoptosis as evidenced by a strong decrease of annexin-V-FITC positive thymocytes. This effect was (1) specific of myoid cells compared to thymic epithelial cells; (2) dependent on direct cell-to-cell contacts and (3) triggered rapidly after 2 h in cocultures. This protective phenomenon was due to the activation of prosurvival mechanisms. Indeed, myoid cells activated extracellular-regulated kinases (ERK1/2) and Akt in thymocytes. Myoid cells also influenced thymocyte maturation. We observed an increase in CD4(+) and a decrease in CD8(+) single positive (SP) thymocytes when cocultured with myoid cells, independently of a CD8(+)SP increased death or a CD4(+)SP overproliferation. Consequently, thymic myoid cells protect thymocytes from apoptosis and could also modulate their differentiation process.


Subject(s)
Apoptosis , Cell Differentiation , Signal Transduction , Thymus Gland/cytology , Thymus Gland/metabolism , Annexin A5/metabolism , Blotting, Western , Cell Line, Transformed , Coculture Techniques , Enzyme Activation , Flow Cytometry , Fluorescein-5-isothiocyanate , Fluorescent Dyes , Humans , Mitogen-Activated Protein Kinase 1/metabolism , Mitogen-Activated Protein Kinase 3/metabolism , Protein Serine-Threonine Kinases/metabolism , Proto-Oncogene Proteins/metabolism , Proto-Oncogene Proteins c-akt , Time Factors
6.
Mol Cell Biol ; 18(4): 1844-54, 1998 Apr.
Article in English | MEDLINE | ID: mdl-9528756

ABSTRACT

Anisomycin, a translational inhibitor secreted by Streptomyces spp., strongly activates the stress-activated mitogen-activated protein (MAP) kinases JNK/SAPK (c-Jun NH2-terminal kinase/stress-activated protein kinase) and p38/RK in mammalian cells, resulting in rapid induction of immediate-early (IE) genes in the nucleus. Here, we have characterized this response further with respect to homologous and heterologous desensitization of IE gene induction and stress kinase activation. We show that anisomycin acts exactly like a signalling agonist in eliciting highly specific and virtually complete homologous desensitization. Anisomycin desensitization of a panel of IE genes (c-fos, fosB, c-jun, junB, and junD), using epidermal growth factor (EGF), basic fibroblast growth factor, (bFGF), tumor necrosis factor alpha (TNF-alpha), anisomycin, tetradecanoyl phorbol acetate (TPA), and UV radiation as secondary stimuli, was found to be extremely specific both with respect to the secondary stimuli and at the level of individual genes. Further, we show that anisomycin-induced homologous desensitization is caused by the fact that anisomycin no longer activates the JNK/SAPK and p38/RK MAP kinase cascades in desensitized cells. In anisomycin-desensitized cells, activation of JNK/SAPKs by UV radiation and hyperosmolarity is almost completely lost, and that of the p38/RK cascade is reduced to about 50% of the normal response. However, all other stimuli produced normal or augmented activation of these two kinase cascades in anisomycin-desensitized cells. These data show that anisomycin behaves like a true signalling agonist and suggest that the anisomycin-desensitized signalling component(s) is not involved in JNK/SAPK or p38/RK activation by EGF, bFGF, TNF-alpha, or TPA but may play a significant role in UV- and hyperosmolarity-stimulated responses.


Subject(s)
Anisomycin/pharmacology , Calcium-Calmodulin-Dependent Protein Kinases/metabolism , Mitogen-Activated Protein Kinases , Nucleic Acid Synthesis Inhibitors/pharmacology , Proto-Oncogene Proteins c-fos/biosynthesis , Proto-Oncogene Proteins c-jun/biosynthesis , Signal Transduction/drug effects , Animals , Cells, Cultured , Enzyme Activation , Epidermal Growth Factor/pharmacology , Fibroblast Growth Factor 2/pharmacology , JNK Mitogen-Activated Protein Kinases , Mice , Mice, Inbred C3H , Osmolar Concentration , Tetradecanoylphorbol Acetate/pharmacology , Tumor Necrosis Factor-alpha/pharmacology , Ultraviolet Rays , p38 Mitogen-Activated Protein Kinases
7.
Eur J Pharmacol ; 307(3): 339-45, 1996 Jul 04.
Article in English | MEDLINE | ID: mdl-8836623

ABSTRACT

In this study, we demonstrated that epidermal growth factor (EGF) stimulated the phosphorylation of myelin basic protein (MBP), a mitogen-activated protein kinase (MAPK) substrate, in crude extracts of human dermal fibroblasts. Moreover, using a selective protein kinase C inhibitor, GF 109203X (3-[1-[3-(dimethylamino)propyl]-1 H-indol-3-yl]-4 (1 H-indol-3-yl)-1 H-pyrrole-2,5-dione monohydrochloride), we observed that protein kinase C was partially involved in the total MBP phosphorylation. To determine the role of protein kinase C in the MBP phosphorylation, we separated, using fast protein liquid chromatography, the proteins present in the fibroblast crude extracts; we thus detected two distinct MBP kinase activities. The first one was stimulated by EGF and corresponded to p42mapk and p44mapk isoforms; this stimulation was not modified by GF 109203X. The second MBP kinase activity was not stimulated by EGF and was due to two protein kinase C isoforms reacting with an anti-protein kinase C zeta antibody. These results show that, in human dermal fibroblasts, EGF stimulates p42mapk and p44mapk isoforms in a protein kinase C-independent manner.


Subject(s)
Calcium-Calmodulin-Dependent Protein Kinases/metabolism , Epidermal Growth Factor/pharmacology , Protein Kinase C/physiology , Skin/enzymology , Adult , Cells, Cultured , Chromatography, Liquid , Enzyme Activation/drug effects , Enzyme Inhibitors/pharmacology , Female , Fibroblasts/enzymology , Humans , Immunoblotting , Indoles/pharmacology , Maleimides/pharmacology , Myelin Basic Protein/metabolism , Phosphorylation , Protein Kinase C/antagonists & inhibitors , Skin/cytology
8.
Exp Dermatol ; 5(2): 108-14, 1996 Apr.
Article in English | MEDLINE | ID: mdl-8734920

ABSTRACT

In this study, we analysed the modulation of keratinocyte growth factor (KGF) mRNA expression in human dermal fibroblasts cultured either in monolayer or within a collagen matrix (dermal equivalent). In monolayer cultures, KGF expression by quiescent fibroblasts was stimulated by different growth substances such as serum, epidermal growth factor and basic fibroblast growth factor. Moreover, we demonstrated that the induction of this gene was mediated by at least 2 different signalling pathways involving protein kinase C (PKC) and cAMP. In dermal equivalents, we observed that the collagen matrix negatively modulated KGF mRNA expression. Indeed, among the growth substances used, only the serum slightly stimulated KGF expression. Nevertheless, as in monolayers, this induction involved at least PKC and cAMP signalling pathways. As the collagen matrix can modulate fibroblast growth, we also studied KGF expression in growing fibroblasts from either monolayer cultures or dermal equivalents. We then showed that this collagen matrix negatively influenced KGF expression independently of the proliferative state of fibroblasts. All these results underline the fact that KGF mRNA expression by human dermal fibroblasts is induced by different substances; however this expression can be modulated by fibroblast-matrix interactions.


Subject(s)
Fibroblast Growth Factors , Growth Substances/biosynthesis , RNA, Messenger/biosynthesis , Skin/metabolism , Transcription, Genetic , Adolescent , Adult , Breast , Cell Division , Cells, Cultured , Cholera Toxin/pharmacology , Culture Media, Conditioned , Enzyme Inhibitors/pharmacology , Epidermal Growth Factor/pharmacology , Female , Fibroblast Growth Factor 10 , Fibroblast Growth Factor 7 , Fibroblasts/cytology , Fibroblasts/drug effects , Fibroblasts/metabolism , Humans , Indoles/pharmacology , Keratinocytes , Maleimides/pharmacology , Middle Aged , Protein Kinase C/antagonists & inhibitors , Signal Transduction , Skin/cytology , Surgery, Plastic , Tetradecanoylphorbol Acetate/pharmacology , Transcription, Genetic/drug effects
9.
Biochem Biophys Res Commun ; 208(1): 245-52, 1995 Mar 08.
Article in English | MEDLINE | ID: mdl-7534073

ABSTRACT

Epidermal growth factor (EGF), 20 ng/ml, stimulated myelin basic protein (MBP) phosphorylation in crude extracts from human keratinocyte primary cultures. In order to identify the involved kinases, we separated by fast protein liquid chromatography proteins participating in MBP phosphorylation. We detected three MBP kinase activities in the keratinocyte crude extracts. The first MBP kinase activity was the only one stimulated by EGF and reacted with anti-mitogen-activated protein kinase (MAPK) antiserum recognising p42mapk and p44mapk isoforms. However, when protein kinase C (PKC) was either inhibited by the PKC inhibitor GF 109203X or depleted by a prolonged TPA treatment, the stimulation of MBP phosphorylation by EGF was strongly inhibited. The second MBP kinase activity eluted was due to a PKC isoform reacting with an anti-PKC zeta antibody, and the third was not identified. With this work, we have thus shown that, in human keratinocytes, EGF activates MAPK activity by a PKC-dependent pathway.


Subject(s)
Calcium-Calmodulin-Dependent Protein Kinases/metabolism , Epidermal Growth Factor/pharmacology , Keratinocytes/enzymology , Protein Kinase C/metabolism , Skin/enzymology , Cells, Cultured , Chromatography, High Pressure Liquid , Cytosol/enzymology , Humans , Immunoblotting , Keratinocytes/drug effects , Kinetics , Myelin Basic Protein/isolation & purification , Myelin Basic Protein/metabolism , Phosphorylation
10.
Biochem Biophys Res Commun ; 204(3): 1081-7, 1994 Nov 15.
Article in English | MEDLINE | ID: mdl-7980581

ABSTRACT

In this study, epidermal growth factor (EGF), 20 ng/ml, and keratinocyte growth factor (KGF), 10 ng/ml, were able to stimulate human keratinocyte growth only in presence of GF 109203X 1 microM, a selective PKC inhibitor. This suggests that PKC negatively controls keratinocyte growth in response to EGF and KGF. On the other hand, EGF and KGF have no significant effect on PKC activity. In contrast, in human fibroblasts, EGF stimulated fibroblast growth in the presence or not of GF 109203X. Thus, EGF seems to stimulate fibroblast growth in a PKC independent manner. Moreover, EGF didn't modify significantly PKC activity in fibroblasts. KGF had no effect on fibroblasts. These results show differences in the interconnections between PKC and EGF transduction pathway in the modulation of human keratinocyte and fibroblast growth. Moreover, they especially demonstrate that PKC would negatively control human keratinocyte growth in response to EGF and also to KGF.


Subject(s)
Cell Division/drug effects , Epidermal Growth Factor/pharmacology , Fibroblast Growth Factors , Growth Substances/pharmacology , Indoles/pharmacology , Keratinocytes/cytology , Maleimides/pharmacology , Protein Kinase C/metabolism , Adult , Cells, Cultured , Dose-Response Relationship, Drug , Drug Interactions , Fibroblast Growth Factor 10 , Fibroblast Growth Factor 7 , Fibroblasts/cytology , Fibroblasts/drug effects , Humans , Keratinocytes/drug effects , Protein Kinase C/antagonists & inhibitors , Skin/cytology
11.
Mol Pharmacol ; 46(3): 445-51, 1994 Sep.
Article in English | MEDLINE | ID: mdl-7935324

ABSTRACT

Protein kinase C (PKC) is known to be involved in cellular proliferation and differentiation. In this work, we have investigated the effects of a novel PKC inhibitor, GF 109203X, on normal human fibroblast and keratinocyte growth. GF 109203X selectively inhibited PKC activity extracted from either fibroblasts (IC50 = 0.01 microM) or keratinocytes (IC50 = 0.4 microM). The inhibitory effects of GF 109203X on total PKC activity and Ca(2+)-independent PKC activity were similar. Nevertheless, in keratinocytes Ca(2+)-independent PKC activity represented 95% of total PKC activity, whereas in fibroblasts it corresponded to only 32% of total PKC activity. GF 109203X also inhibited a cellular function related to PKC activity in living fibroblasts and keratinocytes; it blocked the inhibitory effect of 12-O-tetradecanoylphorbol-13-acetate on 125I-epidermal growth factor binding. GF 109203X inhibited fibroblast growth, in terms of tritiated thymidine incorporation and cell counts, in a dose-dependent manner. We also observed that GF 109203X at 1 microM inhibited serum stimulation of expression of mRNA for c-fos and c-jun, which are usually involved in cellular proliferation. These results suggest that PKC stimulates fibroblast growth. In contrast, GF 109203X stimulated keratinocyte growth. We also observed that GF 109203X inhibited c-fos and c-jun mRNA expression in these cells. In fact, in keratinocytes these proto-oncogenes would be involved in the cellular differentiation process rather than in cellular proliferation. This suggests that the inhibition of PKC favors keratinocyte proliferation probably by inhibiting their differentiation. Thus, using GF 109203X, we show that PKC is involved differently in human fibroblast and keratinocyte growth.


Subject(s)
Fibroblasts/drug effects , Indoles/pharmacology , Keratinocytes/drug effects , Maleimides/pharmacology , Protein Kinase C/antagonists & inhibitors , Adolescent , Adult , Blotting, Northern , Casein Kinase II , Cell Differentiation/drug effects , Cell Differentiation/genetics , Cell Division/drug effects , Cells, Cultured , Cyclic AMP-Dependent Protein Kinases/metabolism , Epidermal Growth Factor/metabolism , Female , Fibroblasts/cytology , Fibroblasts/enzymology , Gene Expression/drug effects , Gene Expression/genetics , Genes, fos/genetics , Genes, jun/genetics , Glyceraldehyde-3-Phosphate Dehydrogenases/genetics , Humans , Keratinocytes/cytology , Keratinocytes/enzymology , Middle Aged , Protein Binding/drug effects , Protein Kinase C/metabolism , Protein Serine-Threonine Kinases/metabolism , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Tetradecanoylphorbol Acetate/pharmacology
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