ABSTRACT
Potential probiotic yeast strains isolated from fermented food need to meet safe and beneficial conditions for the host's health. The Pichia kudriavzevii YGM091 strain isolated from fermented goat milk has outstanding probiotic characteristics, including: the high survival percentage in digestive system conditions (reaching up 247.13 ± 0.12 and 145.03 ± 0.06% at pH 3.0 and bile salt 0.5%, respectively); good tolerance to temperature, salt, phenol, ethanol; good surface properties such as high hydrophobicity percentage (> 60%), the high auto-aggregation percentage rate (66.56 ± 1.45% after 45 min of incubation) and the high co-aggregation percentage rate with pathogenic bacteria in a short time (> 40% after 2 h of incubation); biofilm forming after 24 h of incubation on abiotic surfaces; antioxidant activity reached excellent level after only 24 h of incubation (The percentage free radical scavenging and the Trolox equivalent reaching up 79.86 ± 0.70% and 92.09 ± 0.75 µg/mL after 72 h of incubation); extracellular enzymes production protease and cellulase with high activity, amylase and pectinase with moderate activity and non-lipase activity. Simultaneously, the YGM091 strain is the in vitro safety yeast: insensitive to antibiotics and fluconazole, negative for gelatinase, phospholipase, coagulase, and non-hemolysis activities. Furthermore, this strain is in vivo safety yeast with the dosages below 106 CFU/larva in the Galleria mellonella model with over 90% survival larvae and the yeast density reduced to just 102-103 CFU/larva after 72 h post-injection. Research results have demonstrated that the Pichia kudriavzevii YGM091 strain is a safe potential probiotic yeast and could become a candidate probiotic food to be used in the future.
ABSTRACT
This article present dataset that reflects the perception of extracurricular activities and positive development among youth at 12 schools in Vietnam. In addition, the dataset also explores the relationship between extracurricular activities and positive youth development in Vietnam context. The dataset was collected from adolescents living in three provinces in Vietnam, including: Ha Noi, Nam Dinh, Nghe An and took place in March 2021. The final dataset included 416 fulfilled responses and was used for quantitative analysis through three steps, including: test coefficient alpha, exploratory factors analysis; confirming factors analysis; structural equation modelling. The dataset was obtained as a reference source for future research on positive youth development.
ABSTRACT
The molecular technique for 168 rRNA gene sequences analysis and Western Blot were applied for determination of the Bacillus probiotic strains used in biosubtyl production at IVAC. The results showed that the nucleotide sequence identity of the NT strains was 99.74% in comparison with B. pumilus and the identity of the DL strains was 99.61% in comparison with B. cereus strains from the GenBank/EMBL/DDBJ Database. The result of Western blot analysis showed that, the antigen components of the strains NT and DL were not similar in their antigens, and they were not similar to the B. subtilis ATCC6633.
Subject(s)
Bacillus , Blotting, WesternABSTRACT
Biochemical characteristics of B. subtilis Du, B. subtilis NT, B. subtilis ATCC 6633 were primarily the same, but their size was smaller with small colonies, R form and opaquely white color. 72 hours was culture duration for obtaining highest amount of amylase in B. subtilis ATCC, while in B. subtilis NT and B. subtilis Du – 96 hours
Subject(s)
Bacillus , Bacillus subtilis , BiochemistryABSTRACT
The sporulation of Bacillus subtilis varied depending on the difference between the strains the culture duration and the medium conditions. Edward medium was observed as proper for the sporulation. 48 hours is proper duration for B subtilis Du and ATCC6633, 72 hours for B subtilis NT
