Automated Virtual Fencing Can Effectively Contain Sheep: Field Trials and Prospects.

Virtual fencing technology uses on-animal devices to communicate boundaries via a warning audio tone and electrical pulse signals. There is currently limited validation work on sheep. This study used modified cattle eShepherd® virtual fencing neckbands on reduced-wool sheep with clipped necks to enable automated trials with small groups across both day and night. The first 5-day trial with six Dorper crossbred sheep was conducted in an experimental paddock setting, with a second 5-day trial conducted with 10 Ultra White sheep on a commercial farm. The animals across both trials were contained in the inclusion zone for 99.8% and 92.2% of the trial period, with a mean percentage (±SD) of total audio cues as audio only (i.e., not followed by an electrical pulse) being 74.9% ± 4.6 in the first trial, and 83.3% ± 20.6 for the second trial. In the second trial, sheep crossed over into the exclusion zone on the third night and remained there until they were walked out for their daily yard check in the morning. These preliminary trial results are promising for the use of automated technology on sheep, but suitable devices and algorithms still need to be designed specifically for sheep in the long term.

Beef Cattle Preference and Usage of Environmental Enrichments Provided Simultaneously in a Pasture-Based Environment.

Environmental enrichment can improve livestock welfare through increasing environmental complexity to promote a greater range of natural behaviours. However, there is limited understanding of the need for and impacts of enrichments for extensively managed beef cattle that can sometimes be kept in grassed paddocks devoid of additional natural and artificial features, i.e., 'barren pastures'. This trial assessed which enrichments beef cattle preferred and utilised in a barren paddock environment. Eight groups of seven Angus steers housed on pastured paddocks devoid of natural or artificial features were observed during daylight hours for two days a week over a period of three weeks, after being presented with four enrichments simultaneously: a cattle brush, a piece of hanging rope, a tree stump, and a woodchip pile. Although enrichment use generally decreased over time, the brush, stump, and woodchip maintained a higher level of use than the rope, based on the frequency of interactions and number of displacements around the enrichments (both p < 0.001). This suggests that the brush, stump, and woodchip pile were more valuable resources to the cattle, allowing for grooming and lying behaviours, although oral manipulations also occurred on the stump, woodchip, and rope. The inclusion of these enrichments can increase the complexity of barren pasture environments and allow for the increased expression of natural behaviours, potentially contributing to improved welfare.

Behavior and welfare impacts of water provision via misting in commercial Pekin ducks.

Ducks will access water to maintain feather condition and exhibit natural water-related behaviors such as wet preening. Providing water to ducks commercially is challenging as it may reduce litter and air quality leading to higher duck mortality or illness. This research aimed to measure the behavioral and welfare impacts of water provision via a misting system for commercial Pekin grower ducks in Victoria, Australia. Seven grower flocks were observed (four misted and three nonmisted in open-sided sheds) during May and November 2021. From 26 until 33 d of age, treatment ducks were provided 1 h of misting with shed curtains closed in both treatment and control sheds. At the start and end of the misting application period, external health and welfare measures were taken directly on the ducks via transect walks throughout each shed and catch-and-inspect observations on a sample of 150 ducks from each shed. Video recordings were also made of the misted and nonmisted ducks for 3 h representing time periods prior to, during, and after the 1-h misting across all sheds for all 8 d of the treatment period. Observations were made of all behavior that ducks exhibited at 10-min scan sample intervals across four cameras per shed, totaling 4,198 scans across the seven sheds. General linear mixed models showed the misting application predominantly had impacts on the patterns of behavioral change across the treatment time periods between the misted and nonmisted ducks rather than increasing or decreasing the overall expression of specific behaviors (interaction terms all P ≤ 0.003). The misted ducks increased drinking, tail wagging, and walking, and reduced preening, rooting litter, sitting, and stretching during misting relative to what they showed prior. The nonmisted ducks showed less sitting and more panting during misting relative to prior. Pearson's Chi-square tests showed some differences between the treatment groups in feather cleanliness on the back and wings (both P < 0.0001), likely resulting from pre-existing differences between sheds in blood from pin feathers. Most welfare indicators showed no positive or negative effect of the misting treatment. These results indicate overhead misting does affect duck behavior to some degree without compromising their welfare, but further research with larger water droplet sizes resulting in greater accumulation of surface water or extended durations of misting may lead to greater effects.

Ducks use bathing water for wet preening and feather maintenance. Commercially, it is challenging to provide clean bathing water without compromising litter quality and duck health. Overhead misting may be a mode of water delivery that will wet the ducks' bodies with continuously clean water. This study compared seven grower flocks of Pekin ducks (four misted and three nonmisted treatment flocks) in open-sided sheds during May and November 2021 in Australia. From 26 until 33 d of age, treatment ducks were provided 1 h of misting with shed curtains closed in both treatment and control sheds. External welfare measures were taken directly on the ducks at 26 and 33 d of age. Daily video recordings were made to observe if behaviors differed before, during, or after the 1 h of misting in both treatment and control sheds. Results showed the misting application predominantly affected the way behaviors changed across time between the misted and nonmisted ducks rather than increasing or decreasing the overall expression of specific behaviors. The differences may have in part been related to the curtain closure. Most welfare indicators showed no positive or negative effect of the misting treatment. Larger water droplet sizes may have greater effects on duck behavior.

Relationship between sunlight and range use of commercial free-range hens in Australia.

In Australia, summer brings intense, bright sunlight with high ultraviolet (UV) radiation and hot temperatures, which might impact free-range hens' ranging outside. To determine how range use was correlated with different sunlight variables and weather factors, a study was carried out on three commercial free-range layer farms during the summer/autumn period (December-May) across diverse regions of Australia in Tasmania (TAS), Queensland (QLD), and Western Australia (WA). Hens' range distribution was determined by counting the number of hens in the direct sunlight ('sun') or 'cloud' and shaded areas ('sun-shade' or 'cloud-shade', in sunny or cloudy conditions, respectively) using image snapshots taken at 30 min intervals from video recordings of a portion of one shed comprising 20,000-30,000 hens on each farm during the production phase of the laying cycle. The solar radiation spectrum [UV radiation (UVAB) (288-432 nm), photosynthetically active radiation (PAR; visible light) (400-700 nm), and total solar radiation (TSR) (285 nm-3000 nm)] and weather data (ambient temperature and relative humidity) were recorded through an on-site weather station. Data were analysed separately due to discrepancies between the farms' layouts. The effects of time of day and months on range use were analysed using General Linear Models in JMP® 16.0 and the relationship of sunlight and weather variables with hens' distribution in 'sun'/'sun-shade' and 'cloud'/'cloud-shade' in sunny and cloudy conditions respectively was determined by fitting linear ridge regression models using the 'lmridge' package in R. Overall, the time of day and month had significant effects on hens' distribution on the range (all p < 0.0001). Hens' range occupancy in the 'sun' decreased during the midday period with gradual increases in the late afternoon to evening, and the opposite pattern in the 'sun-shade'. A linear increase in the number of hens on the range over the months indicated the seasonal effects on hen ranging patterns. Temperature, UVAB and PAR were the most important factors for discouraging hens' range use in the 'sun' suggesting free-range systems in Australia should be designed to account for the extreme sunlight using adequate shade for optimum ranging across summer.

Impacts of Rearing Enrichments on Pullets' and Free-Range Hens' Positive Behaviors across the Flock Cycle.

Enrichment during the indoor rearing of pullets destined for free-range systems may optimize pullet development including increasing motivated natural behaviors (termed 'positive behaviors') including foraging, dust bathing and chick play. Hy-Line Brown® chicks (n = 1700) were floor-reared indoors across 16 weeks with three enrichment treatments (n = 3 pens/treatment): (1) standard control, (2) weekly novel objects-'novelty', (3) perching/navigation structures-'structural'. At 16 weeks, pullets (n = 1386) were transferred to nine identical pens within rearing treatments with outdoor range access from 25 to 65 weeks. Video cameras recorded the pullet pens, adult indoor pens, and outside range. During rearing, observations of play behavior (running, frolicking, wing-flapping, sparring) in chicks at 2, 4 and 6 weeks (total of 432 thirty-second scans: 16 observations × 3 days × 9 pens) showed no overall effect of rearing treatment (p = 0.16). At 11 and 14 weeks only the 'novelty' hens were observed to increase their foraging across age (p = 0.009; dust bathing: p = 0.40) (total of 612 thirty-second scans per behavior: 17 observations × 2 days × 2 age points × 9 pens). Observations of adult hens at 26, 31, 41, 50, 60 and 64 weeks showed that the structural hens exhibited overall more dust bathing and foraging than the control hens (both p < 0.04) but both novelty and/or structural hens showed small increases depending on the behavior and location (total of 4104 scans per behavior: 17 observations × 2 days × 6 age points × 9 pens × 2 locations = 3672 + an additional 432 observations following daylight saving). Across age, adult hens differed in the degree of dust bathing performed inside or outside (both p ≤ 0.001) and foraging outside (p < 0.001) but not inside (p = 0.15). For litter-reared pullets, additional enrichments may result in some long-term increases in positive behaviors.

Commercial Free-Range Laying Hens' Preferences for Shelters with Different Sunlight Filtering Percentages.

Extreme sunlight might be aversive to free-range laying hens, discouraging them from going outside. Range enrichment with artificial shelters may protect hens from sunlight and increase range use. The preferences of 34-40-week-old Hy-Line Brown laying hens for artificial shelters were assessed by counting the number of hens under three densities of individual shelters (three replicates/density) from video recordings for 14 to 17 days for two flocks. The artificial shelters used shade cloth marketed as blocking 50%, 70%, and 90% of ultraviolet light, although other sunlight wavelengths were also reduced. Different sunlight spectral irradiances (ultraviolet radiation (UVAB) (288-432 nm), photosynthetically active radiation (PAR) (400-700 nm), and total solar radiation (TSR) (285 nm-3000 nm), ambient temperature, and relative humidity were recorded with an on-site weather station. There was a significant interaction between sunlight-filtering shelter and time of day (both Flocks, p < 0.0001), i.e., hens preferred shelters with the highest amount of sunlight-filtering at most time points. Regression models showed that the most variance in shelter use throughout the day resulted from the ambient temperature in both flocks, while sunlight parameters had different degrees of effect depending on the shelter type and flock. However, fewer hens under the shelters during the midday period suggest that during periods of intense sunlight, hens prefer to remain indoors, and artificial structures might not be sufficient to attract more hens outside.

Virtual Fence Responses Are Socially Facilitated in Beef Cattle.

Group-living can be socially advantageous where the behavior of individuals may be modified by group members through socially facilitative processes. Virtual fencing contains cattle by providing audio and electrical signals via a neckband device. However, little is known about social influences on learning to appropriately respond to the virtual fence (VF) cues. This study aimed to determine whether cattle respond to the behavior of conspecifics during their initial interactions with a VF across 3 days. Sixty-four Angus steers, naïve to virtual fencing, were placed into 8 paddocks (8 animals/group), divided with a VF into two areas- an inclusion and exclusion zone. The animals received an audio cue if they approached the VF followed by an electrical pulse if they continued into the exclusion zone. The GPS and audio and electrical stimuli data were recorded. To quantify social facilitation, individual VF interactions were grouped into 179 "events" across 3 days; starting from when the first animal (leader) approached the VF. The responses of other animals were categorized as (1) followed the leader to move into the exclusion zone (followers, F), (2) accompanied the leader back into the inclusion zone (facilitated, Fa), (3) did not show any reaction (non-facilitated, NFa). A social facilitation score (SFaS) was calculated as SFaS (%) = (F/(Fa+NFa+F)) * 100. A single leader animal led on average 37% of events with 76.2% of all reactions categorized as facilitated by other individuals. Animals responded to the behavior of conspecifics more during the VF implementation compared with facilitated movement during natural grazing patterns when no VF was present (P < 0.001). On average, cattle stopped or turned away to 3.8 (± 2.9 SE) audio cues before ever receiving their first electrical pulse. There was a positive correlation (R = 0.34, P = 0.006) between the number of audio cues received prior to the first electrical pulse and the proportion of all audio cues that were not followed by an electrical pulse. In conclusion, cattle stayed within the inclusion zone based on the response of conspecifics, including some social impacts on individual rates of associative learning between the audio and electrical cues.

Analysis of Cattle Social Transitional Behaviour: Attraction and Repulsion.

Understanding social interactions in livestock groups could improve management practices, but this can be difficult and time-consuming using traditional methods of live observations and video recordings. Sensor technologies and machine learning techniques could provide insight not previously possible. In this study, based on the animals' location information acquired by a new cooperative wireless localisation system, unsupervised machine learning approaches were performed to identify the social structure of a small group of cattle yearlings (n=10) and the social behaviour of an individual. The paper first defined the affinity between an animal pair based on the ranks of their distance. Unsupervised clustering algorithms were then performed, including K-means clustering and agglomerative hierarchical clustering. In particular, K-means clustering was applied based on logical and physical distance. By comparing the clustering result based on logical distance and physical distance, the leader animals and the influence of an individual in a herd of cattle were identified, which provides valuable information for studying the behaviour of animal herds. Improvements in device robustness and replication of this work would confirm the practical application of this technology and analysis methodologies.

Virtual Fencing Technology Excludes Beef Cattle from an Environmentally Sensitive Area.

The eShepherd® virtual fencing system being commercialized for cattle has the potential to exclude cattle from environmentally sensitive areas. Animals are given audio cues to indicate a fence line via a neckband device. An electrical pulse is administered if the animal continues moving forward following an audio cue. A commercial trial was conducted in South Australia to assess whether virtual fencing technology could exclude 20 cattle from an area of regenerating saplings; across 44 days; using a contoured fence line. The results showed that the cattle were able to rapidly learn the virtual fencing cues; responding appropriately to the audio cue for 74.5% of 4378 audio signals; and were excluded from the regenerating area for 99.8% of the trial period with the more complex fence line (contoured; not straight) in place. IceQube R's® measuring lying time and bouts showed no consistent increasing or decreasing pattern of change. At the trial conclusion; the feed available in the protected zone was double the quantity and quality of the grazed zone. Technical issues occurred with some of the pre-commercial prototype devices; but those versions are now obsolete. This study observed a single group of cattle in one paddock; further testing of the virtual technology is warranted.

Virtual Fencing Is Comparable to Electric Tape Fencing for Cattle Behavior and Welfare.

Virtual fencing technology restricts animal movement via communicated signals without physical boundaries. Specifically, the eShepherd™ automated virtual fencing system operates via GPS technology and provides stimuli via a neckband device. An audio warning tone is emitted at the virtual boundary which is followed by an electrical pulse if the animal continues moving forward. Animal welfare is a priority consideration for the commercial implementation of virtual fencing systems. The current study assessed the effects of a virtual fence, in comparison to an electric tape fence, to contain eight groups of eight 12-14 month old steers within a 6-ha area across eight separate paddocks for 4 weeks following 1 week acclimation to the paddocks. Cattle were assessed across two cohorts (four groups/cohort) from January until March 2019 in Australia. Body weight and fecal samples from each animal were taken weekly. Fecal samples were processed for fecal cortisol metabolite (FCM) concentrations. IceQube R®'s fitted to the leg measured individual lying and standing time and the virtual fencing neckbands recorded GPS location and all administered audio and electrical stimuli. Cattle were maintained within their allocated area by both fence types across the 4-week period and those with the virtual fences were responding correctly to the audio cue with an average of 71.51 ± 2.26% of all cues across all animals being audio only. There was individual variation in rate of learning. The electric tape groups in cohort 1 showed a greater increase in body weight over 4 weeks than the virtual fence groups (P < 0.001) but this difference was not confirmed in cohort 2. The fence type statistically influenced the total daily lying time (P = 0.02) with less lying in cattle from the virtual fence groups but this difference equated to an average of <20 min per day. There were no differences between fence types in FCM concentrations (P = 0.39) and the concentrations decreased across time for all cattle (P < 0.001). These results indicate that virtual fencing technology effectively contains animals in a prescribed area across 4 weeks without substantial behavioral and welfare impacts on the cattle.

Temporary Exclusion of Cattle from a Riparian Zone Using Virtual Fencing Technology.

Grazing cattle can both negatively and positively impact riparian zones, dependent on controlled grazing management. Virtual fencing technology, using collar devices that operate via GPS can provide audio cues and electrical stimuli to temporarily exclude cattle from specified areas as desired. An early experimental prototype automated virtual fencing system was tested in excluding ten cattle from a riparian zone in Australia. Animals were given free access to an 11.33-hectare area for three weeks, excluded from river access by a virtual fence for ten days (2.86-hectare inclusion zone), followed by free access again for six days. Animals were almost exclusively contained by the virtual fence. All animals received audio cues and electrical stimuli with daily fence interactions, but there was high individual variation with some animals first approaching the fence more often than others. Overall, there was an approximately 25% probability that animals would receive an electrical stimulus following an audio cue. Individual associative learning may have been socially-facilitated by the group's behaviour. Following fence deactivation, all animals re-entered the previously excluded area. Further research with more groups and longer periods of exclusion using updated collar devices would determine the scope of virtual fencing technology for cattle grazing control.

Relationship between Rectal Temperature and Vaginal Temperature in Grazing Bos taurus Heifers.

This study evaluated the relationship between rectal temperature (TREC, °C) and vaginal temperature (TVAG, °C) in grazing Bos taurus heifers, to develop an understanding of the reliability of these measures as estimates of core body temperature. Nineteen Angus heifers (BW = 232.2 ± 6.91 kg) were implanted with intra-rectal and intra-vaginal data loggers. Rectal temperature and TVAG were simultaneously recorded at 20 s intervals over 18.5 h. Heifers were housed as a singular cohort on grazing pastures for the duration of the study. A strong linear relationship (R² = 0.72, p < 0.0001) between the measurement sites was identified. The mean difference between TREC and TVAG was small, in which TVAG was on average 0.22 ± 0.01 °C lower than TREC. Individual twenty second TREC and TVAG data were used to determine the pooled mean TREC and TVAG and then to highlight the within measure variation over time. The coefficient of variation was, on average, lower (p < 0.001) for TVAG (0.38%) than TREC (0.44%), indicating that TVAG exhibited less variation. Overall, the results from the current study suggest that a strong relationship exists between TREC and TVAG, and that TVAG may be a more reliable estimate of core body temperature than TREC in grazing Bos taurus heifers.

Tech-Savvy Beef Cattle? How Heifers Respond to Moving Virtual Fence Lines.

Global Positioning System (GPS)-based virtual fences offer the potential to improve the management of grazing animals. Prototype collar devices utilising patented virtual fencing algorithms were placed on six Angus heifers in a 6.15 hectare paddock. After a "no fence" period, sequential, shifting virtual fences restricted the animals to 40%, 60%, and 80% of the paddock area widthways and 50% lengthways across 22 days. Audio cues signaled the virtual boundary, and were paired with electrical stimuli if the animals continued forward into the boundary. Within approximately 48 h, the cattle learned the 40% fence and were henceforth restricted to the subsequent inclusion zones a minimum of 96.70% (±standard error 0.01%) of the time. Over time, the animals increasingly stayed within the inclusion zones using audio cues alone, and on average, approached the new fence within 4.25 h. The animals were thus attentive to the audio cue, not the fence location. The time spent standing and lying and the number of steps were similar between inclusion zones (all p ≥ 0.42). More lying bouts occurred at the 80% and lengthways inclusion zones relative to "no fence" (p = 0.04). Further research should test different cattle groups in variable paddock settings and measure physiological welfare responses to the virtual fencing stimuli.