ABSTRACT
The bone wasting induced by estrogen deficiency is, in part, a consequence of increased T cell production of the osteoclastogenic cytokine TNF-alpha. This phenomenon is due to an expansion of T cells, but the responsible mechanism is unknown. We now show that ovariectomy (ovx) disregulates T lymphopoiesis and induces bone loss by stimulating, through a rise in IL-7 levels, both thymic-dependent differentiation of bone marrow-derived progenitors and thymic-independent, peripheral expansion of mature T cells. Attesting to the relevance of the thymic effects, thymectomy decreases by approximately 50% the bone loss and the stimulation of T lymphopoiesis induced by ovx. In contrast, in vivo attenuation of the elevated IL-7 completely prevents the stimulation of T lymphopoiesis and the bone loss that follow ovx. Thus, the disruption of both T cell and bone homeostasis induced by ovx is mediated by IL-7 and due to both the thymic and extrathymic mechanisms. We conclude that IL-7 is a pivotal upstream target through which estrogen regulates hematopoietic and immune functions that are critical for bone homeostasis.
Subject(s)
Estrogens/deficiency , Interleukin-7/physiology , Osteoporosis/etiology , T-Lymphocytes/cytology , Thymus Gland/cytology , Animals , Base Sequence , DNA Primers , Female , Homeostasis , Interleukin-7/biosynthesis , Lymphocyte Activation , Mice , Mice, Inbred C57BL , Osteoporosis/immunology , Ovariectomy , T-Lymphocytes/immunology , Thymus Gland/immunology , Tumor Necrosis Factor-alpha/biosynthesisABSTRACT
Chagas disease is caused by persistent Trypanosoma cruzi infection in muscle tissue that ultimately results in chronic inflammation and tissue destruction. It is unclear why T. cruzi is cleared from some tissues but persists in others, despite an active inflammatory response. In this study, we show that the majority of CD8(+) T cells present in muscle tissue express memory and effector cell surface markers but have sharply attenuated effector function compared with their splenic counterparts. The dysfunction of CD8(+) T cells in the muscle tissue suggests a mechanism by which T. cruzi can persist in that location and cause inflammatory damage.