ABSTRACT
BACKGROUND: Mesenchymal progenitor cells (MPCs or mesenchymal stem cells, MSC) have the capability for differentiation into various lineages of mesenchymal tissue. MPCs are widely distributed in a variety of tissues in the adult human body and also present in the fetal environment. However, MPCs are a rare population in these tissues. In this study we evaluated the possibility that MPCs or cells with MPC-like potency are present in the umbilical cord (UC). METHODS: Term UCs were collected and stored in sterile saline solution. The UCs (10 cm) were cut into 1 cm length, the vessels were striped manually and the tissue immersed in an enzyme cocktail for 3 h at 37 degrees C. The isolated umbilical cord mesenchymal progenitor cells (UCMPCs) were pelleted by low speed centrifugation, suspended and cultured. RESULTS: (1) Umbilical cord mesenchymal progenitor cells (UMPCs) could be isolated in sufficient quantities and (2) could be cultured easily. (3) These cells demonstrated a fibroblast-like phenotype. (4) They could be expanded in culture and induced to form several different types of cells. (5) In immunochemistry these cells express mesenchymal markers (CD 13, CD 105) but not haematopoetic lineage markers (CD 14 and CD 34). CONCLUSION: Our observation suggested that MPCs are present in human umbilical cord. Instead, it should be considered a valuable resource for the isolation of potent cells for cell-based therapies, especially in general and pediatric surgery.
Subject(s)
Mesenchymal Stem Cells/cytology , Umbilical Cord/cytology , Cell Culture Techniques , Cell Differentiation , Cell Lineage , Cell Separation , Cells, Cultured , Centrifugation , Fibroblasts/cytology , Humans , Immunochemistry , PhenotypeABSTRACT
This study investigated the impact of three types of support--from family and friends, the workplace, and community services agencies--on the perceived stress and health outcomes of 133 employed caregivers of dependent parents. The stressor-support-stress-reaction model guided the research. Support from family and friends and community agencies was found to buffer the negative impact of caregiving and work and family role strain, and a supportive work environment was one predictor of lessened physical strain.
Subject(s)
Caregivers/psychology , Home Nursing/psychology , Social Support , Stress, Psychological/prevention & control , Activities of Daily Living , Aged , Community Health Services , Cross-Sectional Studies , Dependency, Psychological , Employment , Female , Health Status , Humans , Male , Middle Aged , Models, Psychological , New York City/epidemiology , Risk Factors , Role , Stress, Psychological/epidemiology , Stress, Psychological/psychologyABSTRACT
A 22 year-old woman with diabetes insipidus on chronic therapy with desmopressin acetate (DDAVP) developed recurrent venous thromboembolism and transient thrombocytopenia temporally related to the administration of DDAVP. Large increases in plasma von Willebrand factor (vWF), vWF-activity, and relative increases in the concentrations of the larger multimeric forms of vWF-antigen were observed, as well as a plasma factor which sensitized normal platelets to undergo spontaneous aggregation in vitro. Additional studies showed that the patient's plasma retained the platelet aggregation inducing activity after selective removal of vWF by immunoabsorption. The nature of the platelet activating factor and the relationship of this factor and the excessively increased and transiently abnormal vWF to the recurrent venous thromboembolism in this patient remain uncertain. Although the findings do not implicate definitively DDAVP in the elevation of vWF in this patient, it is suggested that its use be considered with caution in patients with diabetes mellitus and increased levels of vWF.