ABSTRACT
We report on the chiral separation of pharmacologically active dihydropyrimidinones by capillary electrophoresis (CE) using carboxymethyl-beta-cyclodextrin as chiral selector. The influence of selector concentration, pH, and the addition of varying amounts of methanol is investigated. Out of 21 compounds investigated, 19 were resolved, 13 with baseline separation.
Subject(s)
Cyclodextrins , Electrophoresis, Capillary/methods , Pyrimidinones/isolation & purification , beta-Cyclodextrins , Cyclodextrins/chemistry , Hydrogen-Ion Concentration , Indicators and Reagents , Pyrimidinones/chemistry , StereoisomerismABSTRACT
This paper deals with the enantioseparation of hydroxy acids by ligand-exchange capillary electrochromatography. A chiral continuous bed was easily prepared by in situ polymerization of monomers, including an L-4-hydroxyproline derivative. This phase showed chiral recognition for several hydroxy acids, in addition to amino acids.
Subject(s)
Electrophoresis, Capillary/methods , Hydroxy Acids/isolation & purification , Amino Acids/chemistry , Amino Acids/isolation & purification , Hydroxy Acids/chemistry , Ligands , Mandelic Acids/chemistry , Mandelic Acids/isolation & purification , Polymers , StereoisomerismABSTRACT
This review summarizes the application of capillary electrophoresis and capillary electrochromatography for the chiral separation of various substance classes using the principle of ligand exchange. The application of this principle to various substance classes is reported.
Subject(s)
Chromatography/methods , Electrophoresis, Capillary/methods , Ligands , Models, Chemical , Stereoisomerism , Time FactorsABSTRACT
The application of the principle of ligand-exchange capillary electrophoresis to two substance classes is described. As chiral selector N-(2-hydroxyoctyl)-L-4-hydroxyproline-copper(II) complex was used. This principle was applied to the chiral separation of alpha-hydroxy acids and drugs containing amino alcohol structure such as beta-blockers. The enantioselectivity was found to be strongly dependent on pH corresponding to the optimal conditions for complex formation for each structure class.
