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1.
Food Chem ; 245: 981-988, 2018 Apr 15.
Article in English | MEDLINE | ID: mdl-29287469

ABSTRACT

Feed sustainability is one of the biggest challenges for the next few years. Solutions have to be found that take feed quality and safety into account. Animal by-products are one valuable source of proteins. However, since the bovine spongiform encephalopathy (BSE) crisis, their use has been strictly regulated. The objective of this study was to propose a routine, sensitive and specific method using ultra-high performance liquid chromatography coupled to tandem mass spectrometry for the detection of blood-derived products and milk powder in feed. Contaminated aquafeeds were analysed in order to evaluate the sensitivity and specificity of the method. This new method meets both selectivity and sensitivity (0.1% (w/w)) requirements imposed by the European Commission for animal proteins detection methods. It offers an innovative and complementary solution for the simultaneously identification of authorised and unauthorised animal by-products such as processed animal proteins (PAPs).


Subject(s)
Animal Feed/analysis , Blood , Limit of Detection , Milk/chemistry , Tandem Mass Spectrometry/methods , Animals , Cattle , Chromatography, High Pressure Liquid , Food Contamination/analysis , Time Factors
2.
Food Chem ; 213: 417-424, 2016 Dec 15.
Article in English | MEDLINE | ID: mdl-27451199

ABSTRACT

Animal by-products are valuable protein sources in animal nutrition. Among them are blood products and blood meal, which are used as high-quality material for their beneficial effects on growth and health. Within the framework of the feed ban relaxation, the development of complementary methods in order to refine the identification of processed animal proteins remains challenging. The aim of this study was to identify specific biomarkers that would allow the detection of bovine blood products and processed animal proteins using tandem mass spectrometry. Seventeen biomarkers were identified: nine peptides for bovine plasma powder; seven peptides for bovine haemoglobin powder, including six peptides for bovine blood meal; and one peptide for porcine blood. They were not detected in several commercial compound feed or feed materials, such as blood by-products of other animal origins, milk-derived products and fish meal. These biomarkers could be used for developing a species-specific and blood-specific detection method.


Subject(s)
Animal Feed/analysis , Blood Proteins/analysis , Spectrometry, Mass, Electrospray Ionization/methods , Tandem Mass Spectrometry/methods , Amino Acid Sequence , Animals , Biomarkers/analysis , Biomarkers/blood , Blood Proteins/genetics , Cattle , Chromatography, High Pressure Liquid/methods , Dairy Products/analysis , Encephalopathy, Bovine Spongiform/diagnosis , Encephalopathy, Bovine Spongiform/prevention & control , Food Contamination/analysis , Hemoglobins/analysis , Hemoglobins/genetics , Poultry , Species Specificity , Swine
3.
Sci Rep ; 4: 5730, 2014 Jul 17.
Article in English | MEDLINE | ID: mdl-25034259

ABSTRACT

Molecular biology techniques such as PCR constitute powerful tools for the determination of the taxonomic origin of bones. DNA degradation and contamination by exogenous DNA, however, jeopardise bone identification. Despite the vast array of techniques used to decontaminate bone fragments, the isolation and determination of bone DNA content are still problematic. Within the framework of the eradication of transmissible spongiform encephalopathies (including BSE, commonly known as "mad cow disease"), a fluorescence in situ hybridization (FISH) protocol was developed. Results from the described study showed that this method can be applied directly to bones without a demineralisation step and that it allows the identification of bovine and ruminant bones even after severe processing. The results also showed that the method is independent of exogenous contamination and that it is therefore entirely appropriate for this application.


Subject(s)
Animal Feed , Bone and Bones/chemistry , DNA/genetics , Animals , Cattle , DNA Probes/genetics , In Situ Hybridization, Fluorescence
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