ABSTRACT
Phenylmercuric chloride was applied in three doses (5 ppm, 30 ppm Hg, and 30 ppm Hg + 4 ppm Se) via the food for 60 days. The effect of Hg with an without Se was studied histologically and the data of a shortened spermatogram were evaluated. Treatment with 30 ppm Hg resulted in hypospermia, occurrence of abnormally maturing spermatozoa, reduction of the volume of semen, and decrease in the number of spermatozoa. The dose of 5 ppm Hg only resulted in the appearance of abnormally developing cells and decreased sperm motility. The addition of Se maintained spermatogenesis and the values of semen on the control level.
Subject(s)
Chickens , Phenylmercury Compounds/toxicity , Seminiferous Epithelium/drug effects , Animals , Male , Phenylmercury Compounds/administration & dosage , Selenium/administration & dosage , Selenium/toxicity , Semen/drug effects , Seminiferous Epithelium/pathology , Sperm Motility/drug effects , Spermatozoa/drug effects , Spermatozoa/ultrastructureABSTRACT
Ejaculate of cooks was diluted by the Wishart and Davtjan diluents at a ratio of 1 : 1 and stored at a temperature of 4 degrees C during 2, 24, 48 and 72 hours before insemination of laying hens by the doses of 25, 50, 75, 100 and 150. 10(6) spermatozoa. With both diluents, egg fertility higher than 90 per cent was gained after 48 hours of keeping the ejaculate in sperm doses of 75 and 100 . 10(6) spermatozoa in the case of both diluents.
Subject(s)
Chickens , Fertilization , Semen Preservation/veterinary , Sperm Count/veterinary , Animals , Insemination, Artificial/veterinary , Male , Time FactorsABSTRACT
Two experiments were performed to evaluate the influence of supercooling and temperature of ejaculate crystallization in white broad-breasted male turkeys, hybrid Ivagal, on the motility, proportion of live and morphologically normal spermatozoa. As follows from the results obtained by testing the quality of spermatozoa, increasing supercooling and decreasing temperature of crystallization have a destructive effect.
Subject(s)
Spermatozoa/physiology , Temperature , Turkeys , Animals , Cold Temperature , Crystallization , Male , Sperm Motility , Spermatozoa/cytologyABSTRACT
Ethylene glycol at concentrations of 8 + 8, 4 + 12 or 0 + 16% was added to the ejaculate of the White Broad-Breasted turkey-stags by the method of (ejaculate : diluent) 1 : (1 + 1), 1 : (1 + 1/4 + 1/4 + 2/4) or 1 : (1 + 1/15 + 1/15 + 1/15 + 2/15 + + 4/15 + 6/15) at temperatures of 25, 15 or 5 degrees C. The dissolved ejaculate was equilibrated for 15 minutes, three minutes it was left to freeze in liquid nitrogen vapours and 24 hours it was stored at a temperature of -196 degrees C. Motility, percentage of live spermatozoa and percentage of morphologically normal spermatozoa were evaluated after sample thawing at 15 degrees C. The quality of cryo-preserved spermatozoa of stags was most effectively influenced by the addition of ethylene glycol to ejaculate at the concentration of 0 + 16% by the method of 1 : (1 + 1/15 + 1/15 + + 1/15 + 2/15 + 4/15 + 6/15) at the temperature of 25 degrees C.
Subject(s)
Ethylene Glycols , Semen Preservation/methods , Turkeys , Animals , Cell Survival , Ethylene Glycol , Freezing , Male , Sperm Motility , Spermatozoa/cytologyABSTRACT
After exposure of sexually immature chickens to lindan in doses of 2.5 to 25.0 mg kg-1 of feeds, the effect of the insecticide on the biological activity of estradiol was investigated. The changes in the effectiveness of estrogen, expressed by an increase of the mass of the oviduct and of the level of serum calcium, were evaluated by means of comparison of animals exposed to the insecticide with a control group that had been applied only the standard hormone dose. Proofs were obtained for the fact that the application of lindan in the quantity of the mentioned doses for 14 days caused a statistically conclusive depression of the biological effectiveness of estrogens. Compared with the control group, the stimulated mass of the oviduct of animals exposed to the insecticide was smaller by 12.7 to 17.1 p. c. and the average concentration of the serum calcium decreased by 8.0 to 27.6 p. c. The results obtained indicate that the influence of lindan already in relatively low concentrations caused significant changes in the effectiveness of exogenous estrogens.
