Tracheal replacement revisited: Use of a vascularized tracheal transplant in a porcine model.

OBJECTIVES/HYPOTHESIS: To determine if a long segment of trachea can be transplanted as a vascularized organ and to determine if a tracheal transplant is a potential surgical option for a long-segment circumferential tracheal defect. STUDY DESIGN: Animal model. METHODS: Four (two donors and two recipients) adult domestic Yorkshire swine were used. Two sets of transplants were performed from a donor to recipient pig. The transplant was placed heterotopically (not in continuity with the airway), and the recipient animals were monitored for 14 days to ensure the transplants were well vascularized. Immunosuppressive therapies included methylprednisolone, cyclosporine, and azathioprine. Gross as well as histological examination of multiple tissues types including mucosa, cartilage, muscle, and blood vessels were performed postsacrifice on day 14. RESULTS: Recipient animal weights ranged from 40 to 42 kilograms. Both recipient pigs survived the full 14 days of study and exhibited normal activity and appetite. Ischemia time of transplanted grafts ranged from 63 to 72 minutes. Transplanted tracheas included a minimum of 15 cartilaginous rings and measured greater than 10 cm in length. Both grafts maintained a robust blood supply throughout the duration of study. CONCLUSIONS: The entire visceral compartment can be reliably transplanted, either as a single component (trachea) or as a chimeric flap with multiple components (trachea, esophagus, larynx, and pharynx). Further studies in the swine model should be considered to study the effects of transplanting the trachea orthotopically into the native airway. Further studies are needed into the reliability of this technique of transplantation in humans. LEVEL OF EVIDENCE: NA Laryngoscope, 128:S1-S9, 2018.

The changing picture of high-grade anal intraepithelial neoplasia in men who have sex with men: the effects of 10 years of experience performing high-resolution anoscopy.

BACKGROUND: Ten years ago, we published findings from anal dysplasia screening in a metropolitan surgical practice where the majority of men who have sex with men had biopsy-proven high-grade anal intraepithelial neoplasia. OBJECTIVE: This study aimed to determine the effect of 10 years of experience in anal dysplasia screening on the prevalence of high-grade anal intraepithelial neoplasia. DESIGN: A retrospective chart review was performed of all anal cytology results of 1189 men who have sex with men screened in a 1-year period, with subsequent high-resolution anoscopy and biopsy as necessary. PATIENTS: The patients studied were men who have sex with men. MAIN OUTCOME MEASURE: The main outcome measure was biopsy-proven high-grade anal intraepithelial neoplasia. RESULTS: : There were 315 (37.2%) biopsy-verified instances of high-grade anal intraepithelial neoplasia. Regression analysis determined that age, HIV status, infection by high-risk human papillomavirus, and abnormal cytology results were significant predictors of high-grade anal intraepithelial neoplasia. In a 1-year period, the number of men who have sex with men screened was nearly 7 times greater than in the 2-year period studied 10 years earlier. LIMITATIONS: We did not separately analyze patients who had previously been treated for high-grade anal intraepithelial neoplasia. CONCLUSIONS: Severity of cytology and infection with high-risk human papillomavirus are the most significant predictors of high-grade anal intraepithelial neoplasia, underscoring the importance of anal dysplasia screening. Our ability to identify high-grade anal intraepithelial neoplasia has improved with 10 years of experience performing high-resolution anoscopy.

Predictors of anal dysplasia in men who have sex with men with benign cytology.

PURPOSE: Anal cytology is used to screen patients for anal dysplasia. Anal pap smears are collected without direct visualization of the canal, whereas cervical cytology is obtained with direct visualization. Current screening guidelines dictate that patients with abnormal cytology are to be evaluated with high-resolution anoscopy, and patients with benign anal cytology forgo high-resolution anoscopy. We routinely perform standard anoscopy after cytology collection. We endeavored to determine whether our standard procedure would reveal findings supporting high-resolution anoscopy even with benign cytology. METHODS: We performed a retrospective chart review of all anal cytology results from January 2008 to March 2009, identifying the patients with benign results who had undergone high-resolution anoscopy. Records were analyzed to determine the indication for high-resolution anoscopy and biopsy results. RESULTS: We identified 2084 patients with anal cytology results, including 613 (29%) patients who had benign results and 169 (28%) patients who had undergone high-resolution anoscopy. The main reasons for high-resolution anoscopy were high risk of recurrent dysplasia based on prior high-grade squamous intraepithelial lesion with cytology done concurrently in 77 (46%), and abnormal anoscopy findings thought to be indicative of dysplasia (plaque or nodule suspicious of dysplasia, internal or external condyloma, dysplastic appearing fissure) in 92 (54%). Of subjects with abnormal anoscopy findings indicative of dysplasia, 25 (27%) had high-grade and 39 (42%) had low-grade dysplasia. Of those undergoing cytology and high-resolution anoscopy simultaneously, 14 (18%) had high-grade and 12 (16%) had low-grade dysplasia. In total, 90 (53%) of those with benign cytology who had high-resolution anoscopy had abnormal pathology, and 39 (23%) had high-grade dysplasia. CONCLUSIONS: Men having sex with men can have significant dysplasia with benign cytology. Standard anoscopy identifies findings indicative of dysplasia that, if present, should lead to high-resolution anoscopy even with benign cytology. Standard anoscopy should become an integral part of anal dysplasia screening. Prior recent history of high-grade dysplasia should lead to high-resolution anoscopy even with benign cytology.