In vivo assessment of hypertensive nephrosclerosis using ultrasound localization microscopy.

PURPOSE: As a typical chronic kidney disease (CKD), hypertensive nephrosclerosis (HN) is a common syndrome of hypertension, characterized by chronic kidney microvascular damage. Early diagnosis of microvascular damage using conventional ultrasound imaging encounters challenges in sensitivity and specificity owing to the inherent diffraction limit. Ultrasound localization microscopy (ULM) has been developed to obtain microvasculature and microvascular hemodynamics within the kidney, and would be a promising tool for the early diagnosis of CKD. METHODS: In this study, the advantage of quantitative indexes obtained by using ULM (mean arterial blood flow speeds of different segments of interlobular arteries) over indexes obtained using conventional clinical serum (ß2-microglobulin, serum urea nitrogen, and creatinine) and urine (24-h urine volume and urine protein) tests and ultrasound Doppler imaging (peak systolic velocity [PSV], end-diastolic velocity [EDV], and resistance index [RI]) and contrast-enhanced ultrasound imaging (CEUS; rise time [RT], peak intensity [IMAX], mean transit time [mTT], and area under the time-intensity curve [AUC]) for early diagnosis of HN were investigated. Examinations were carried out on six spontaneously hypertensive rats (SHR) and five normal Wistar-Kyoto (WKY) rats at the age of 10 weeks. RESULTS: The experimental results show that the indicators derived from conventional clinical inspections (serum and urine tests) and ultrasound imaging (PSV, EDV, RI, RT, IMAX, mTT, and AUC) do not show significant difference between hypertensive and healthy rats (p > 0.05), while the TTP of the SHR group (28.52 ± 5.52 s) derived from CEUS is significantly higher than that of the WKY group (18.68 ± 7.32 s; p < 0.05). The mean blood flow speed in interlobular artery of SHR (12.47 ± 1.06 mm/s) derived from ULM is significantly higher than that of WKY rats (10.13 ± 1.17 mm/s; p < 0.01). CONCLUSION: The advantages of ULM over conventional clinical inspections and ultrasound imaging methods for early diagnosis of HN were validated. The quantitative results show that ULM can effectively diagnose HN at the early stage by detecting the blood flow speed changes of interlobular arteries. ULM may promise a reliable technique for early diagnosis of HN in the future.

Evaluating HIFU-mediated local drug release using thermal strain imaging: Phantom and preliminary in-vivo studies.

PURPOSE: High-intensity focused ultrasound (HIFU)-mediated drug release becomes a promising therapeutic technique for treatment of cancer, which has merits of deep penetration, noninvasive approach and nonionizing radiation. However, conventional thermocouple-based approach for treatment monitoring would encounter big challenges such as the viscous heating artifact and difficulty in monitoring in the deep region. In this study, we develop an effective method based on thermal strain imaging (TSI) for the evaluation of HIFU-mediated drug release. METHODS: Both phantom experiments and preliminary animal experiments were performed to investigate the feasibility of the proposed approach. Doxorubicin (DOX)-loaded cerasomes (HIFU and temperature-sensitive cerasomes, HTSCs) were prepared. In the phantom experiments, the HTSC solution is contained inside a cylindrical chamber within a tissue-mimicking phantom. In the animal experiments, the HTSCs are intravenously injected into tumor-bearing mice. An HIFU transducer is used to trigger DOX release from the HTSCs within the phantom or mice, and TSI is performed during HIFU heating. In the phantom experiments, the accuracy of temperature estimation using TSI is validated by measuring with a thermocouple. In animal experiments, the spatial consistency between the distribution of DOX released within the tumor and the location of the heating region estimated by TSI is validated using a spectrofluorophotometer. RESULTS: In the phantom experiments, the HTSCs show a burst release of DOX when the temperature of the HTSC solution estimated by TSI reaches about 42°C, which is in agreement with the condition for drug release from the HTSCs. The temperature estimation using TSI has high accuracy with error below 2.5%. In animal experiments, fluorescence imaging of the tumor validates that the heating region of HIFU could be localized by the low-strain region of TSI. CONCLUSION: The present framework demonstrates a reliable and effective solution to the evaluation of HIFU-mediated local drug delivery.

Electromagnetic tracking-based freehand 3D quasi-static elastography with 1D linear array: a phantom study.

Recent developments in hardware and scanning protocols have advanced conventional 2D quasi-static elastography to 3D level, which provides an intuitive visualization of lesions. A 2D linear array or scanning mechanism is typically required for 3D quasi-static elastography, requiring expensive and specifically designed hardware. In this study, we propose a novel method based on a commercial electromagnetic tracking system for freehand 3D quasi-static elastography with 1D linear array. Phantom experiments are performed to validate the feasibility of the proposed method. During data acquisition, the probe contacts the surface of an elasticity phantom and moves in the elevational direction, while applying sinusoidal-like axial compression to the phantom. For each frame of ultrasound data, the 3D coordinates and orientations of the probe are obtained from an electromagnetic tracking system. A correlation-based algorithm is adopted to obtain a series of axial strain images. Volumetric strain data are reconstructed by using the recorded 3D coordinates and orientations of the probe corresponding to each strain image. The diameters of inclusions are then obtained from the slice plots of the volumetric strain data. The volumes of inclusions are estimated from the isosurface plots. The experimental result shows that the volume estimation of the inclusions has good accuracy, with errors within 2%, while the diameters of the inclusions estimated from three orthogonal planes have larger errors up to 18%. In conclusion, the present framework would promise a reliable and effective solution for freehand 3D quasi-static elastography with 1D linear array.

Novel Method for Vessel Cross-Sectional Shear Wave Imaging.

Many studies have investigated the applications of shear wave imaging (SWI) to vascular elastography, mainly on the longitudinal section of vessels. It is important to investigate SWI in the arterial cross section when evaluating anisotropy of the vessel wall or complete plaque composition. Here, we proposed a novel method based on the coordinate transformation and directional filter in the polar coordinate system to achieve vessel cross-sectional shear wave imaging. In particular, ultrasound radiofrequency data were transformed from the Cartesian to the polar coordinate system; the radial displacements were then estimated directly. Directional filtering was performed along the circumferential direction to filter out the reflected waves. The feasibility of the proposed vessel cross-sectional shear wave imaging method was investigated through phantom experiments and ex vivo and in vivo studies. Our results indicated that the dispersion relation of the shear wave (i.e., the guided circumferential wave) within the vessel can be measured via the present method, and the elastic modulus of the vessel can be determined.

Infrared thermal imaging system on a mobile phone.

A novel concept towards pervasively available low-cost infrared thermal imaging system lunched on a mobile phone (MTIS) was proposed and demonstrated in this article. Through digestion on the evolutional development of milestone technologies in the area, it can be found that the portable and low-cost design would become the main stream of thermal imager for civilian purposes. As a representative trial towards this important goal, a MTIS consisting of a thermal infrared module (TIM) and mobile phone with embedded exclusive software (IRAPP) was presented. The basic strategy for the TIM construction is illustrated, including sensor adoption and optical specification. The user-oriented software was developed in the Android environment by considering its popularity and expandability. Computational algorithms with non-uniformity correction and scene-change detection are established to optimize the imaging quality and efficiency of TIM. The performance experiments and analysis indicated that the currently available detective distance for the MTIS is about 29 m. Furthermore, some family-targeted utilization enabled by MTIS was also outlined, such as sudden infant death syndrome (SIDS) prevention, etc. This work suggests a ubiquitous way of significantly extending thermal infrared image into rather wide areas especially health care in the coming time.