ABSTRACT
A Gram-positive, alkaliphilic bacterium, designated strain BA288(T), was isolated from sandy soil. Cells were facultatively anaerobic, endospore-forming rods that were motile by means of peritrichous flagella. The strain grew at 15-40 degrees C and pH 7.0-11.0 (optimally at 30 degrees C and pH 9.0-9.5) and at salinities of 0-4 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BA288(T) belonged to the genus Bacillus and that Bacillus herbersteinensis D-1,5a(T), Bacillus humi LMG 22167(T), Bacillus cohnii DSM 6307(T) and Bacillus litoralis SW-211(T) were the closest neighbours (96.2, 96.0, 96.0 and 95.9 % sequence similarity, respectively). The genomic DNA G+C content was 37.9 mol% and the predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0), C(16 : 0) and iso-C(14 : 0). The peptidoglycan type was A1gamma (meso-diaminopimelic acid). Therefore, on the basis of phylogenetic, phenotypic and chemotaxonomic properties, strain BA288(T) represents a novel species of the genus Bacillus, for which the name Bacillus alkalitelluris sp. nov. is proposed. The type strain is BA288(T) (=KCTC 3947(T) =DSM 16976(T)).
Subject(s)
Bacillus/classification , Silicon Dioxide , Soil Microbiology , Bacillus/genetics , Bacillus/isolation & purification , Bacillus/physiology , Bacterial Typing Techniques , Base Composition , DNA, Bacterial/analysis , DNA, Ribosomal/analysis , Fatty Acids/analysis , Genes, rRNA , Hydrogen-Ion Concentration , Korea , Molecular Sequence Data , Phenotype , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Species SpecificityABSTRACT
A moderately halophilic, Gram-negative bacterium, designated strain BH843(T), was isolated from a solar saltern in Korea and subjected to a taxonomic analysis. Strain BH843(T) grew at salinities of 1-14 % (w/v) NaCl and at temperatures of 10-40 degrees C. The cells were motile cocci or short rods with single flagella and contained C(16 : 0), C(19 : 0) cyclo omega8c and C(17 : 0) cyclo as the major fatty acids. The G+C content of the genomic DNA was 66 mol% and the predominant ubiquinone was Q-9. Comparative 16S rRNA gene sequence analyses showed that strain BH843(T) formed a distinct phyletic line within the genus Halomonas, and the levels of 16S rRNA gene sequence similarity with respect to recognized Halomonas species were below 95.1 %. The levels of DNA-DNA relatedness between strain BH843(T) and the type strains of phylogenetically closely related Halomonas species were below 25 %. On the basis of phenotypic, chemotaxonomic and molecular data, strain BH843(T) represents a novel species within the genus Halomonas, for which the name Halomonas kribbensis is proposed. The type strain is BH843(T) (=KCTC 12584(T)=DSM 17892(T)).
Subject(s)
Halomonas/classification , Halomonas/isolation & purification , Seawater/microbiology , Base Composition , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Fatty Acids/analysis , Genes, rRNA , Halomonas/chemistry , Halomonas/genetics , Korea , Locomotion/physiology , Molecular Sequence Data , Nucleic Acid Hybridization , Phylogeny , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid , Sodium Chloride/metabolism , Temperature , Ubiquinone/analysisABSTRACT
A strictly aerobic, non-spore-forming, Gram-negative bacterium, designated strain HW7(T), was isolated from a rice field in Korea. Cells of strain HW7(T) were short rod-shaped and motile with single polar flagella. The major cellular fatty acids of strain HW7(T) were C(16 : 0) and summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH). The genomic DNA G+C content was 56 mol% and the major isoprenoid quinone was Q-8. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HW7(T) forms a distinct lineage with respect to closely related genera within the class Betaproteobacteria and that the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 93 %. On the basis of the physiological and phylogenetic data, strain HW7(T) represents a novel genus and species of the Betaproteobacteria, for which the name Leeia oryzae gen. nov., sp. nov. is proposed. The type strain is HW7(T) (=KCTC 12585(T)=DSM 17879(T)).
Subject(s)
Betaproteobacteria/classification , Betaproteobacteria/isolation & purification , Soil Microbiology , Aerobiosis , Base Composition , Betaproteobacteria/genetics , Betaproteobacteria/physiology , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Fatty Acids/analysis , Genes, rRNA , Korea , Locomotion , Molecular Sequence Data , Oryza , Phylogeny , Quinones/analysis , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid , Spores, BacterialABSTRACT
A Gram-positive, spore-forming and moderately halophilic bacterium, strain BH139(T), was isolated from saline sediment of Xinjiang Province, China. Cells of strain BH139(T) were motile, flagellated rods. The organism grew optimally at 30-35 degrees C in the presence 12-14 % (w/v) NaCl. The major fatty acids were branched saturated fatty acids such as iso-C(16 : 0), anteiso-C(15 : 0), iso-C(15 : 0), iso-C(14 : 0) and anteiso-C(17 : 0). The G + C content of the genomic DNA was about 43 mol% and the predominant isoprenoid quinone was MK-7. Comparative 16S rRNA gene sequence analyses showed that strain BH139(T) was most closely related to Lentibacillus salicampi KCCM 41560(T) (96.9 % 16S rRNA gene sequence similarity) and formed a distinct phyletic line from that species. On the basis of physiological and molecular properties, the isolate represents a novel species within the genus Lentibacillus, for which the name Lentibacillus salarius sp. nov. is proposed. The type strain is BH139(T) (= KCTC 3911(T) = DSM 16459(T)).
