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1.
Cardiovasc Intervent Radiol ; 45(10): 1415-1427, 2022 Oct.
Article in English | MEDLINE | ID: mdl-35853955

ABSTRACT

PURPOSE: This study aims to examine outcomes of immature arteriovenous fistula salvage using balloon angioplasty (PTA) without and with accessory vein obliteration (PTA + VO). MATERIALS AND METHODS: PubMed and Embase were accessed on 21 September 2020 to retrieve cohort studies on adult patients with end-stage renal failure (ESRF) requiring dialysis. Risk of bias was assessed using Newcastle-Ottawa Scale. Studies were pooled into PTA or PTA + VO arms, with outcomes (technical/clinical success, primary/secondary post-intervention patency until 12 months) reported as event rates with 95% confidence intervals. Random-effects model and maximum likelihood meta-regression were used for meta-analysis. RESULTS: Fourteen studies (1030 participants) were included. The between-subgroup difference in outcomes was largely non-significant (p > 0.050). CONCLUSION: The evidence does not support balloon angioplasty with concomitant accessory vein obliteration for immature fistula salvage.


Subject(s)
Angioplasty, Balloon , Arteriovenous Fistula , Arteriovenous Shunt, Surgical , Adult , Angioplasty, Balloon/adverse effects , Arteriovenous Fistula/etiology , Arteriovenous Shunt, Surgical/adverse effects , Graft Occlusion, Vascular/surgery , Humans , Renal Dialysis/adverse effects , Retrospective Studies , Treatment Outcome , Vascular Patency
2.
J Prim Prev ; 40(6): 591-606, 2019 12.
Article in English | MEDLINE | ID: mdl-31655950

ABSTRACT

Studies in the U.S. and Mexico have observed the clustering of food resources around schools, which may promote the use of these resources. Our study characterized and compared school neighborhood food environments in Guadalajara, Jalisco, and Houston, Texas, and examined socioeconomic disparities in food resource availability across school neighborhoods. We used the Goods and Services Inventory to document the frequency and type of resources within each school neighborhood. School neighborhoods in Guadalajara had significantly more food resources than those in Houston. We found that convenience stores and table service restaurants were the most prevalent food resources in school neighborhoods in both cities. Guadalajara school neighborhoods had a higher prevalence of supermarkets and grocery stores than Houston. Low-income school neighborhoods in Guadalajara with poorly educated residents had significantly more food carts than high-income neighborhoods with more educated residents. In Houston, we found significantly more fast food restaurants and convenience stores in school neighborhoods with more educated residents than school neighborhoods with less educated residents. The influence of food resources within school neighborhoods on the dietary habits of schoolchildren should be further explored in both the U.S. and Mexico. The characterization of school neighborhood food environments can inform policymakers, city planners, and school officials who seek to implement policies to create healthier food environments.


Subject(s)
Feeding Behavior , Food Supply , Residence Characteristics , Schools , Humans , Mexico , Pediatric Obesity/prevention & control , Restaurants , Texas
3.
Cell Death Dis ; 5: e1261, 2014 May 29.
Article in English | MEDLINE | ID: mdl-24874733

ABSTRACT

One of the most common observations in cell death assays is that not all cells die at the same time, or at the same treatment dose. Here, using the perspective of the systems biology of apoptosis and the context of cancer treatment, we discuss possible sources of this cell-to-cell variability as well as its implications for quantitative measurements and computational models of cell death. Many different factors, both within and outside of the apoptosis signaling networks, have been correlated with the variable responses to various death-inducing treatments. Systems biology models offer us the opportunity to take a more synoptic view of the cell death process to identify multifactorial determinants of the cell death decision. Finally, with an eye toward 'systems pharmacology', we discuss how leveraging this new understanding should help us develop combination treatment strategies to compel cancer cells toward apoptosis by manipulating either the biochemical state of cancer cells or the dynamics of signal transduction.


Subject(s)
Apoptosis , Computer Simulation , Models, Biological , Neoplasms/metabolism , Signal Transduction , Systems Biology/methods , Animals , Humans , Neoplasms/pathology
4.
ISME J ; 7(8): 1632-40, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23552624

ABSTRACT

We present evidence for the directed formation of ice by planktonic communities dominated by filamentous diatoms sampled from the ice-covered Laurentian Great Lakes. We hypothesize that ice formation promotes attachment of these non-motile phytoplankton to overlying ice, thereby maintaining a favorable position for the diatoms in the photic zone. However, it is unclear whether the diatoms themselves are responsible for ice nucleation. Scanning electron microscopy revealed associations of bacterial epiphytes with the dominant diatoms of the phytoplankton assemblage, and bacteria isolated from the phytoplankton showed elevated temperatures of crystallization (T(c)) as high as -3 °C. Ice nucleation-active bacteria were identified as belonging to the genus Pseudomonas, but we could not demonstrate that they were sufficiently abundant to incite the observed freezing. Regardless of the source of ice nucleation activity, the resulting production of frazil ice may provide a means for the diatoms to be recruited to the overlying lake ice, thereby increasing their fitness. Bacterial epiphytes are likewise expected to benefit from their association with the diatoms as recipients of organic carbon excreted by their hosts. This novel mechanism illuminates a previously undescribed stage of the life cycle of the meroplanktonic diatoms that bloom in Lake Erie and other Great Lakes during winter and offers a model relevant to aquatic ecosystems having seasonal ice cover around the world.


Subject(s)
Diatoms/physiology , Ice , Lakes , Phytoplankton/physiology , Bacteria/classification , Bacteria/isolation & purification , Bacteria/ultrastructure , DNA, Ribosomal/genetics , Diatoms/microbiology , Diatoms/ultrastructure , Ecosystem , Microscopy, Electron, Scanning , Molecular Sequence Data , Phytoplankton/microbiology , Temperature
6.
Ir J Med Sci ; 176(1): 37-40, 2007 Mar.
Article in English | MEDLINE | ID: mdl-17849522

ABSTRACT

BACKGROUND: To determine the impact of obtaining routine peritoneal fluid cultures during appendicectomy, on the treatment and the clinical outcomes. METHODS: The case notes of 137 consecutive patients having appendicectomy, selected from the microbiology database over a period of 1 year were reviewed. The microorganisms in peritoneal cultures, selection of antibiotics and clinical outcomes were recorded. Patients were subdivided into two groups; group I: uncomplicated appendicitis and group II: complicated appendicitis. RESULTS: The study included 137 patients with a median age of 19 years. Cultures were obtained from 79.5% of patients (group I: 67/84, group II: 42/53). Cultures were positive in 28.3% (19/67) patients in group I and 69% (29/ 42) in group II. Wound infection (5.6%), prolonged ileus (7.5%) and intra-abdominal abscess (3.7%) were the recorded complications in group II. Antibiotics were modified in 3 out of 109 patients. CONCLUSION: Intra-operative peritoneal cultures during appendicectomy do not significantly contribute towards patient management.


Subject(s)
Abdominal Abscess/diagnosis , Appendectomy/methods , Appendicitis/surgery , Ascitic Fluid/microbiology , Bacterial Infections/drug therapy , Cells, Cultured , Surgical Wound Infection/prevention & control , Abdominal Abscess/prevention & control , Abdominal Cavity , Adolescent , Adult , Anti-Bacterial Agents/therapeutic use , Antibiotic Prophylaxis , Ascitic Fluid/chemistry , Bacterial Infections/microbiology , Cefuroxime/therapeutic use , Child , Child, Preschool , Female , Humans , Length of Stay , Male , Metronidazole/therapeutic use , Middle Aged , Postoperative Complications/prevention & control , Retrospective Studies , Risk Factors
7.
J Comp Physiol B ; 177(2): 205-15, 2007 Feb.
Article in English | MEDLINE | ID: mdl-17115223

ABSTRACT

The seabird tick Ixodes uriae is exposed to extreme environmental conditions during the off-host phase of its life cycle on the Antarctic Peninsula. To investigate how this tick resists desiccation, water requirements of each developmental stage were determined. Features of I. uriae water balance include a high percentage body water content, low dehydration tolerance limit, and a high water loss rate, which are characteristics that classify this tick as hydrophilic. Like other ticks, I. uriae relies on water vapor uptake as an unfed larva and enhanced water retention in the adult, while nymphs are intermediate and exploit both strategies. Stages that do not absorb water vapor, eggs, fed larvae and fed nymphs, rely on water conservation. Other noteworthy features include heat sensitivity that promotes water loss in eggs and unfed larvae, an inability to drink free water from droplets, and behavioral regulation of water loss by formation of clusters. We conclude that I. uriae is adapted for life in a moisture-rich environment, and this requirement is met by clustering in moist, hydrating, microhabitats under rocks and debris that contain moisture levels that are higher than the tick's critical equilibrium activity.


Subject(s)
Ecosystem , Ixodes/physiology , Animals , Antarctic Regions , Body Water/physiology , Dehydration/physiopathology , Feeding Behavior/physiology , Female , Ixodes/growth & development , Life Cycle Stages/physiology , Male , Ovum/physiology , Temperature , Water-Electrolyte Balance/physiology
8.
J Comp Physiol B ; 175(3): 209-17, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15739066

ABSTRACT

Freezing survival in hatchling turtles may be limited by ischemic anoxia in frozen tissues and the associated accumulation of lactate and reactive oxygen species (ROS). To determine whether mechanisms for coping with anoxia are also important in freeze tolerance, we examined the association between capacities for freezing survival and anoxia tolerance in hatchlings of seven species of turtles. Tolerance to freezing (-2.5 degrees C) was high in Emydoidea blandingii, Chrysemys picta, Terrapene ornata, and Malaclemys terrapin and low in Graptemys geographica, Chelydra serpentina, and Trachemys scripta. Hatchlings survived in a N(2) atmosphere at 4 degrees C for periods ranging from 17 d (M. terrapin) to 50 d (G. geographica), but survival time was not associated with freeze tolerance. Lactate accumulated during both stresses, but plasma levels in frozen/thawed turtles were well below those found in anoxia-exposed animals. Activity of the antioxidant enzyme catalase in liver increased markedly with anoxia exposure in most species, but increased with freezing/thawing only in species with low freeze tolerance. Our results suggest that whereas oxygen deprivation occurs during somatic freezing, freeze tolerance is not limited by anoxia tolerance in hatchling turtles.


Subject(s)
Acclimatization/physiology , Animals, Newborn/physiology , Hypoxia/physiopathology , Turtles/physiology , Animals , Catalase/metabolism , Freezing , Lactates/blood , Liver/anatomy & histology , Liver/enzymology , Organ Size , Oxidative Stress/physiology , Species Specificity
9.
Arch Dis Child Fetal Neonatal Ed ; 89(4): F285-8, 2004 Jul.
Article in English | MEDLINE | ID: mdl-15210656

ABSTRACT

Autopsy remains the best method of investigating perinatal deaths. Recent years have, however, seen a decline in autopsy rates. This review looks at some of the options available for investigating perinatal deaths when the family decline to give consent for standard autopsy.


Subject(s)
Autopsy/statistics & numerical data , Death, Sudden/etiology , Informed Consent , Biopsy/methods , Cause of Death , Death, Sudden/pathology , Humans , Infant, Newborn , Laparoscopy/methods , Magnetic Resonance Imaging/methods , Physical Examination , Radiography/methods , Skin/pathology
10.
J Comp Physiol B ; 173(8): 643-51, 2003 Nov.
Article in English | MEDLINE | ID: mdl-12925879

ABSTRACT

We conducted a 3-year field and laboratory study of winter biology in hatchlings of the northern map turtle ( Graptemys geographica). At our study area in northern Indiana, hatchlings routinely overwintered in their natal nests, emerging after the weather warmed in spring. Winter survival was excellent despite the fact that hatchlings were exposed frequently to subfreezing temperatures (to -5.4 degrees C). In the laboratory, cold-acclimated hatchlings exhibited low rates of evaporative water loss (mean=2.0 mg g(-1) day(-1)), which would enable them to conserve body water during winter. Laboratory-reared hatchlings were intolerant of freezing at -2.5 degrees C for 24 h, conditions that are readily survived by freeze-tolerant species of turtles. Winter survival of hatchling G. geographica probably depended on their extensive capacity for supercooling (to -14.8 degrees C) and their well-developed resistance to inoculative freezing, which may occur when hatchlings contact ice and ice-nucleating agents present in nesting soil. Supercooled hatchlings survived a brief exposure to -8 degrees C. Others, held at -6 degrees C for 5 days, maintained ATP concentrations at control levels, although they did accumulate lactate and glucose, probably in response to tissue hypoxia. Therefore, anoxia tolerance, as evidenced by the viability of hatchlings exposed to N(2) gas for 8 days, may promote survival during exposure to subfreezing temperatures.


Subject(s)
Adaptation, Physiological , Hibernation , Turtles/physiology , Acclimatization , Animals , Animals, Newborn , Body Temperature Regulation , Body Water , Dehydration , Female , Freezing , Ice , Seasons , Time Factors
11.
J Insect Physiol ; 49(4): 385-93, 2003 Apr.
Article in English | MEDLINE | ID: mdl-12769992

ABSTRACT

We examined seasonal changes in freeze tolerance and the susceptibility of larvae of the gall fly, Eurosta solidaginis to inoculative freezing within the goldenrod gall (Solidago sp.). In late September, when the water content of the galls was high (approximately 55%), more than half of the larvae froze within their galls when held at -2.5 degrees C for 24 h, and nearly all larvae froze at -4 or -6 degrees C. At this time, most larvae survived freezing at > or = -4 degrees C. By October plants had senesced, and their water content had decreased to 33%. Correspondingly, the number of larvae that froze by inoculation at -4 and -6 degrees C also decreased, however the proportion of larvae that survived freezing increased markedly. Gall water content reached its lowest value (10%) in November, when few larvae froze during exposure to subzero temperatures > or = -6 degrees C. In winter, rain and melting snow transiently increased gall water content to values as high as 64% causing many larvae to freeze when exposed to temperatures as high as -4 degrees C. However, in the absence of precipitation, gall tissues dried and, as before, larvae were not likely to freeze by inoculation. Consequently, in nature larvae freeze earlier in the autumn and/or at higher temperatures than would be predicted based on the temperature of crystallization (T(c)) of isolated larvae. However, even in early September when environmental temperatures are relatively high, larvae exhibited limited levels of freezing tolerance sufficient to protect them if they did freeze.


Subject(s)
Adaptation, Physiological , Body Water/metabolism , Cold Temperature , Diptera/physiology , Larva/metabolism , Larva/physiology , Seasons , Animals , Body Weight , Diptera/metabolism , Freezing , Hemolymph/metabolism , Osmolar Concentration , Rain
12.
Br J Cancer ; 87(10): 1092-8, 2002 Nov 04.
Article in English | MEDLINE | ID: mdl-12402147

ABSTRACT

One aspect of concern for survivors of Wilms' tumour has been the late outcome in terms of renal function. Previous studies have documented low glomerular filtration rate and high blood pressure in some patients. Furthermore, disorders in tubular function (especially urinary concentration defects) have been suggested but not confirmed in small studies. The aim of this study was to determine the prevalence and nature of subclinical and overt glomerular, proximal and distal renal tubular toxicity in a population based cohort of survivors of Wilms' tumour. Forty patients (24 female) with a median age of 4.3 years (3 months-11.8 years) at diagnosis were studied. Median follow-up was 8.8 (range 0.06-27.5) years. Glomerular filtration rate was measured by (51)Cr-EDTA plasma clearance, proximal tubular function by electrolyte fractional excretions, urine excretion of low molecular weight proteins (retinol-binding protein) and renal tubular enzymes (alanine aminopeptidase; N-acetylglucosaminidase) and distal tubular function by the osmolality of the first two urines of the day on 3 consecutive days. Renal size (ultrasound) and blood pressure were also measured. Mean (range) glomerular filtration rate was 100 (61-150) ml min(-1) 1.73 m(-2). Nine were below the reference range for healthy individuals with two kidneys. Most serum electrolyte concentrations (sodium, potassium, chloride, calcium, magnesium and phosphate) fell within the normal range for age, as did the fractional excretions. The values that fell outside the normal range were only marginally abnormal. Subclinical measures of tubular toxicity (retinal-binding protein, alanine aminopeptidase, N-acetylglucosaminidase) were abnormal in only four patients. Thirty-seven patients achieved maximal urine osmolalities > or =800 mOsm kg(-1), but three failed to achieve this value even after DDAVP administration. Two patients had evidence of increased urinary albumin excretion. Compensatory renal hypertrophy was seen in all but two patients, but blood pressure was within normal limits in all patients. Current and past treatment for Wilms' tumour does not have any clinically important nephrotoxic effect in the majority of patients. This finding will enable paediatric oncologists to reassure patients and parents that treatment for Wilms' tumour rarely causes long-term renal impairment.


Subject(s)
Kidney Neoplasms/physiopathology , Kidney/physiopathology , Wilms Tumor/physiopathology , Age Factors , Blood Pressure , Child , Child, Preschool , Female , Humans , Infant , Kidney Neoplasms/pathology , Kidney Neoplasms/therapy , Kidney Tubules, Distal/physiopathology , Kidney Tubules, Proximal/physiopathology , Male , Survivors , Wilms Tumor/pathology , Wilms Tumor/therapy
13.
Am J Public Health ; 91(12): 2019-21, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11726387

ABSTRACT

OBJECTIVES: These studies investigated (1) the effect of community bans of self-service tobacco displays on store environment and (2) the effect of consumer tobacco accessibility on merchants. METHODS: We counted cigarette displays (self-service, clerk-assisted, clear acrylic case) in 586 California stores. Merchant interviews (N = 198) identified consumer tobacco accessibility, tobacco company incentives, and shoplifting. RESULTS: Stores in communities with self-service tobacco display bans had fewer self-service displays and more acrylic displays but an equal total number of displays. The merchants who limited consumer tobacco accessibility received fewer incentives and reported lower shoplifting losses. In contrast, consumer access to tobacco was unrelated to the amount of monetary incentives. CONCLUSIONS: Community bans decreased self-service tobacco displays; however, exposure to tobacco advertising in acrylic displays remained high. Reducing consumer tobacco accessibility may reduce shoplifting.


Subject(s)
Advertising/legislation & jurisprudence , Community Health Planning/legislation & jurisprudence , Environment , Smoking Cessation/methods , Tobacco Industry/legislation & jurisprudence , California , Humans , Motivation , Theft
14.
Arch Insect Biochem Physiol ; 48(4): 199-205, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11746564

ABSTRACT

Although it is often assumed that survival of freezing requires that ice formation must be restricted to extracellular compartments, fat body cells from freeze-tolerant larvae of the gall fly, Eurosta solidaginis (Diptera, Tephritidae) survive intracellular freezing. Furthermore, these cells are highly susceptible to inoculative freezing by external ice, undergo extensive lipid coalescence upon thawing, and survive freezing better when glycerol is added to the suspension medium. To determine whether these traits are required for intracellular freeze tolerance or whether they are incidental and possessed by fat body cells in general, we investigated the capacity of fat body cells from nondiapause-destined and diapause-destined (i.e., cold-hardy) larvae of the freeze-intolerant flesh fly Sarcophaga crassipalpis (Diptera, Sarcophagidae) to survive intracellular freezing. Fat body cells from both types of larvae were highly susceptible to inoculative freezing; all cells froze between -3.7 to -6.2 degrees C. The highest rates for survival of intracellular freezing occurred at -5 degrees C. The addition of glycerol to the media markedly increased survival rates. Upon thawing, the fat body cells showed little or no lipid coalescence. Fat body cells from E. solidaginis had a water content of only 35% compared to cells from S. crassipalpis larvae that had 52-55%; cells with less water may be less likely to be damaged by mechanical forces during intracellular freezing.


Subject(s)
Diptera/physiology , Fat Body/physiology , Freezing , Animals , Cell Survival/physiology , Diptera/growth & development , Fat Body/chemistry , Lipids/analysis
15.
Women Health ; 32(4): 1-15, 2001.
Article in English | MEDLINE | ID: mdl-11548133

ABSTRACT

Walking for exercise is becoming widely recognized for bestowing health benefits. This study examined the association of walking for exercise and mood in sedentary, ethnic minority women over a five-month period. Ethnic minority women (N = 102) participated in a randomized, controlled trial of a 7-week behaviorally based telephone and mail intervention that promoted the adoption of walking for exercise compared to a non-behavioral minimal intervention. At 2-month post-test and 5-month follow-up, participants reported significant decreases in depressive mood and increases in vigor. Increase in walking over the course of the study was associated with change in vigor. Limited evidence was found to support a relationship between walking for exercise and mood improvement in ethnic-minority women.


Subject(s)
Affect/physiology , Ethnicity/psychology , Exercise/psychology , Health Behavior/ethnology , Health Promotion/methods , Walking/psychology , Women's Health , Adult , Analysis of Variance , Counseling , Female , Humans , Middle Aged , Persuasive Communication , Prospective Studies , Telephone , United States
16.
Tob Control ; 10(2): 184-8, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11387542

ABSTRACT

OBJECTIVE: To examine the extent and types of cigarette advertising materials in stores and to assess tobacco company compliance with the 1998 Master Settlement Agreement (MSA). DESIGN: A cross-sectional analysis of a random sample of 586 stores that sold cigarettes. SETTING: US state of California. MAIN OUTCOME MEASURES: Trained data collectors classified cigarette advertising materials by type (signs, displays, functional items), location (interior or exterior), and placement (below 3 feet (1 m) or near candy). RESULTS: California retail outlets featured 17.2 (SD 16.1) tobacco advertising materials on average, and 94% of stores featured at least some advertising. About 85% of these were within 4 feet (1.3 m) of the counter. About 50% of the stores had ads at or below 3 feet, and 23% had cigarette product displays next to candy. In violation of the MSA, 3% of stores featured signs with cartoons and 11% had large exterior signs. CONCLUSIONS: Tobacco companies are aggressively using stores to market cigarettes. Moreover, the spirit of the MSA-to protect children from cigarette advertising-has not been realised. Future studies should monitor industry use of this venue and assess the impact of exposure to cigarette advertising materials in stores on adult smokers and youth.


Subject(s)
Advertising , Commerce , Smoking Prevention , Tobacco Industry/legislation & jurisprudence , Advertising/legislation & jurisprudence , California , Child , Child Welfare , Cross-Sectional Studies , Humans , Smoking/economics
17.
J Comp Physiol B ; 171(4): 335-40, 2001 May.
Article in English | MEDLINE | ID: mdl-11409631

ABSTRACT

We investigated physiological responses to supercooling in hatchling painted turtles (Chrysemys picta) which remain in their natal nests over winter and therefore may become exposed to subzero temperatures. These turtles are freeze tolerant but also must rely on supercooling to survive exposure to the lower temperatures occurring in nests during winter. We compared whole-body concentrations of lactate, glucose, glycerol, and ATP in turtles chilled at 0 degrees C, -4 degrees C, or -6 degrees C for 5 days, or at 6 degrees C for 19 days. In a companion experiment, we measured metabolite concentrations in turtles exposed to a hypoxic environment for 1 day, 4 days, or 8 days. Supercooling and hypoxia exposure were both associated with an increase in concentrations of lactate and glucose and a decrease in glycerol concentrations (albeit no change in the ATP pool), suggesting that supercooling induces functional hypoxia. We conclude that hypoxia tolerance may be an important pre-adaptation for surviving exposure to subzero temperatures in hatchling C. picta.


Subject(s)
Animals, Newborn/physiology , Cold Temperature , Hypoxia/physiopathology , Turtles/physiology , Animals , Body Temperature/physiology , Glucose/metabolism , Glycerol/metabolism , Lactic Acid/metabolism , Osmolar Concentration , Turtles/growth & development
18.
J Exp Biol ; 204(Pt 9): 1659-66, 2001 May.
Article in English | MEDLINE | ID: mdl-11398755

ABSTRACT

In contrast to most studies of rapid cold-hardening, in which abrupt transfers to low temperatures are used to induce an acclimatory response, the primary objectives of this study were to determine (i) whether rapid cold-hardening was induced during the cooling phase of an ecologically based thermoperiod, (ii) whether the protection afforded was lost during warming or contributed to increased cold-tolerance during subsequent cycles and (iii) whether the major thermally inducible stress protein (Hsp70) or carbohydrate cryoprotectants contributed to the protection afforded by rapid cold-hardening. During the cooling phase of a single ecologically based thermoperiod, the tolerance of Drosophila melanogaster to 1 h at -7 degrees C increased from 5 +/- 5% survival to 62.5 +/- 7.3% (means +/- S.E.M., N=40-60), while their critical thermal minima (CTmin) decreased by 1.9 degrees C. Cold hardiness increased with the number of thermoperiods to which flies were exposed; i.e. flies exposed to six thermoperiods were more cold-tolerant than those exposed to two. Endogenous levels of Hsp70 and carbohydrate cryoprotectants were unchanged in rapidly cold-hardened adults compared with controls held at a constant 23 degrees C. In nature, rapid cold-hardening probably affords subtle benefits during short-term cooling, such as allowing D. melanogaster to remain active at lower temperatures than they otherwise could.


Subject(s)
Acclimatization , Cold Temperature , Drosophila melanogaster/physiology , Periodicity , Aging , Animals , Carbohydrate Metabolism , Cryoprotective Agents/metabolism , Female , HSP70 Heat-Shock Proteins/metabolism , Hot Temperature , Male , Sex Characteristics
19.
J Comp Physiol B ; 171(3): 181-8, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11352100

ABSTRACT

Seasonal changes in metabolic rate and the potential for morphological development demonstrated that third-instar larvae of the goldenrod gall fly, Eurosta solidaginis Fitch, exhibit a distinct winter diapause. Metabolic rate (CO2 production) was significantly lower from 15 October to 9 February than in early autumn (9 September) and spring (1 March) samples. The induction of diapause coincided with the development of cold-hardening, maximum larval mass, and gall senescence, but our experiments did not identify specific cues triggering diapause induction. We examined the influence of exposure to 0 degrees C and -20 degrees C on diapause development. Diapause development in larvae stored at 0 degrees C occurred at approximately the same rate as in nature. Until 15 December the larvae were in the refractory phase of diapause (incapable of morphological development, even at permissive temperatures), but afterward moved to the activated phase within which diapause intensity decreased until termination in February. Diapause development occurred in larvae collected during the winter and stored at -20 degrees C for periods of 1 week to 3 months. Diapause intensity decreased in frozen larvae through the winter but at a slower rate than in larvae stored at 0 degrees C.


Subject(s)
Cold Temperature , Diptera/growth & development , Seasons , Animals , Diptera/metabolism , Freezing , Larva/metabolism , Larva/physiology
20.
Mol Cell Biol ; 21(6): 2221-34, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11238955

ABSTRACT

CTCF is a widely expressed and highly conserved multi-Zn-finger (ZF) nuclear factor. Binding to various CTCF target sites (CTSs) is mediated by combinatorial contributions of different ZFs. Different CTSs mediate distinct CTCF functions in transcriptional regulation, including promoter repression or activation and hormone-responsive gene silencing. In addition, the necessary and sufficient core sequences of diverse enhancer-blocking (insulator) elements, including CpG methylation-sensitive ones, have recently been pinpointed to CTSs. To determine whether a posttranslational modification may modulate CTCF functions, we studied CTCF phosphorylation. We demonstrated that most of the modifications that occur at the carboxy terminus in vivo can be reproduced in vitro with casein kinase II (CKII). Major modification sites map to four serines within the S(604)KKEDS(609)S(610)DS(612)E motif that is highly conserved in vertebrates. Specific mutations of these serines abrogate phosphorylation of CTCF in vivo and CKII-induced phosphorylation in vitro. In addition, we showed that completely preventing phosphorylation by substituting all serines within this site resulted in markedly enhanced repression of the CTS-bearing vertebrate c-myc promoters, but did not alter CTCF nuclear localization or in vitro DNA-binding characteristics assayed with c-myc CTSs. Moreover, these substitutions manifested a profound effect on negative cell growth regulation by wild-type CTCF. CKII may thus be responsible for attenuation of CTCF activity, either acting on its own or by providing the signal for phosphorylation by other kinases and for CTCF-interacting protein partners.


Subject(s)
DNA-Binding Proteins/metabolism , Repressor Proteins , Transcription Factors/metabolism , Amino Acid Sequence , Amino Acid Substitution , Animals , Binding Sites , CCCTC-Binding Factor , Casein Kinase II , Cell Division/genetics , Cell Line , Chickens , DNA-Binding Proteins/genetics , Genes, myc , Humans , Molecular Sequence Data , Mutation , Phosphorylation , Promoter Regions, Genetic , Protein Serine-Threonine Kinases/genetics , Protein Serine-Threonine Kinases/metabolism , Transcription Factors/genetics
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