ABSTRACT
A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05T, was isolated from compost. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055T (96.3â%), Brevibacterium oceani BBH7T (96.2â%) and Brevibacterium epidermidis NBRC 14811T (96.1â%). The DNA G+C content was 62.3 mol%. The predominant quinone was MK-8(H2). The major fatty acids were anteiso-C15â:â0, anteiso-C17â:â0, iso-C16â:â0 and iso-C15â:â0. The cell-wall peptidoglycan of strain BS05T contained meso-diaminopimelic acid. The major polar lipid was phosphatidylglycerol. Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05T from the recognized species of the genus Brevibacterium. Therefore, strain BS05T represents a novel species of the genus Brevibacterium, for which the name Brevibacteriumhankyongi sp. nov. is proposed, with the type strain BS05T (=KACC 18875T=LMG 29562T).
Subject(s)
Brevibacterium/classification , Composting , Phylogeny , Soil Microbiology , Bacterial Typing Techniques , Base Composition , Brevibacterium/genetics , Brevibacterium/isolation & purification , DNA, Bacterial/genetics , Diaminopimelic Acid/chemistry , Fatty Acids/chemistry , Nucleic Acid Hybridization , Peptidoglycan/chemistry , Phosphatidylglycerols/chemistry , RNA, Ribosomal, 16S/genetics , Republic of Korea , Sequence Analysis, DNAABSTRACT
A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated strain KTCe-4T, was isolated from activated sludge. Based on 16S rRNA gene sequencing and phylogenetic analysis, the novel isolate was found to belong to the genus Flavobacterium and was most closely related to Flavobacteriumterrae DSM 18829T (97.8â%), Flavobacteriumvireti THG-SM1T (97.8â%), Flavobacteriumbrevivitae TTM-43T (97.4â%) and shared <96.4â% sequence similarity to the other members of the genus. Strain KTCe-4T contained MK-6 as the predominant isoprenoid quinone and iso-C15â:â0, iso-C15â:â0 G, iso-C15â:â0 3-OH, iso-C17â:â0 3-OH and iso-C17â:â1ω9c, as the major fatty acids. The major polar lipids were phosphatidylethanolamine, two unidentified polar lipids and one unknown amino lipid. The DNA-DNA relatedness values of strain KTCe-4T with respect to type strains of recognized species of the genus Flavobacterium were less than 70â%. Based on 16S rRNA gene sequencing, low values of DNA-DNA hybridization and polyphasic taxonomic analysis, strain KTCe-4T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium hankyongi sp. nov. is proposed. The type strain of Flavobacterium hankyongi is strain KTCe-4T (=KACC 16613T=JCM 18198T).
