Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 8 de 8
Filter
Add more filters










Database
Language
Publication year range
1.
Arch Pharm Res ; 34(9): 1443-50, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21975805

ABSTRACT

In the course of isolating preventive agents against sepsis based on the in vivo assay model, eleven known compounds, (-)-catechin (1), catechin-7-O-ß-apiofuranoside (2), catechin-7-O-α-Lrhamnopyranoside (3), catechin-3-O-α-L-rhamnopyranoside (4), catechin-7-O-ß-D-glucopyranoside (5), butyl (+)-5'-methoxyisolariciresinol-9'-O-ß-D-xylopyranoside (6), lyoniside (7), nudiposide (8), α-nigerose (9), butyl α-D-fructofuranoside (10), and procyanidin B(3) (11) were isolated from the root barks of Ulmus davidiana var. japonica. Compounds 2, 6, and 8 significantly protected against sepsis in a mouse model with survival rates of mice exposed to 10 mg/kg of LPS/D-GalN ranged from 80%-100%. Among them, 8 exhibited the most potent protective effect and decreased the plasma levels of TNF-α, IL-10 and ALT activity.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Plant Extracts/therapeutic use , Shock, Septic/prevention & control , Ulmus/chemistry , Alanine Transaminase/blood , Animals , Anti-Inflammatory Agents, Non-Steroidal/isolation & purification , Chromatography, High Pressure Liquid , Disease Models, Animal , Interleukin-6/blood , Male , Mice , Mice, Inbred ICR , Molecular Structure , Plant Bark/chemistry , Plant Extracts/isolation & purification , Plant Roots/chemistry , Shock, Septic/immunology , Survival Analysis , Tumor Necrosis Factor-alpha/blood
2.
Immunopharmacol Immunotoxicol ; 33(2): 373-9, 2011 Jun.
Article in English | MEDLINE | ID: mdl-20929426

ABSTRACT

We evaluated the ability of the ethylacetate fraction of marine sponge, Cliona celata (ECC), harvested from Korean seaside to regulate the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated murine macrophage-like RAW264.7 cells. ECC dose-dependently inhibited both the expression of iNOS protein and mRNA, resulting in decreased production of nitric oxide (NO), with an IC(50) of 80.5 µg/mL. To investigate action mechanism by which ECC inhibits NO production and iNOS expression, we examined the activation of IκB in LPS-stimulated RAW264.7 cells. ECC clearly inhibited translocation of nuclear factor-κB (NF-κB) p65 subunits from cytosol to nucleus, which correlated with its inhibitory effects on IκB-α phosphorylation and degradation. Furthermore, ECC potently suppressed both the reporter gene expression and DNA-binding activity of NF-κB, which was associated with decreased p65 protein levels in the nucleus. Here, we show for the first time that ECC inhibits NF-κB activation through the inhibition of IκB degradation.


Subject(s)
Acetates/pharmacology , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Macrophages/drug effects , Macrophages/pathology , Porifera/chemistry , Acetates/isolation & purification , Animals , Anti-Inflammatory Agents, Non-Steroidal/isolation & purification , Cell Line , Lipopolysaccharides/toxicity , Macrophages/metabolism , Mice , NF-kappa B/antagonists & inhibitors , NF-kappa B/biosynthesis , Nitric Oxide/antagonists & inhibitors , Nitric Oxide/biosynthesis , Nitric Oxide Synthase Type II/antagonists & inhibitors , Nitric Oxide Synthase Type II/biosynthesis
3.
Arch Pharm Res ; 33(9): 1307-15, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20945128

ABSTRACT

Twenty five compounds including ten triterpenes (1-3, 5-11), six flavonoids (12-15, 24, 25), five lignans (17, 18, 21-23), two butenyl clohexnone glycosides (19-20), one fructofuranoside (16) and one fatty acid (4) were isolated from the roots of Ulmus davidiana var. japonica. The structures of those compounds were identified by comparing their physicochemical and spectral data with those of published in literatures. All the compounds were evaluated for DNA topoisomerase inhibitory activities and cytotoxicities. Among the purified compounds, 4 and 19 showed more potent inhibitory acitivities (IC(50): 39 and 19 µM, respectively) than camptothecin, as the positive control (IC(50): 46 µM) against topoisomerase I. Compounds, 4, 10, 12, 19, 24 and 25 showed strong inhibitory activities toward DNA topoisomerase II (IC(50): 0.1, 0.52, 0.47, 0.42, 0.17 µM and 17 nM, respectively), which were more potent than that of etoposide as positive control (IC(50): 20 µM). In A549 cell line, 5 and 6 showed cytotoxicities (IC(50): 4 µM and 3 µM, respectively, with IC(50) of camptothecin as positive control: 10.3 µM). In the HepG2 cell line, 3, 5 and 7 showed cytotoxicity (IC(50): 4, 3 and 4 µM, respectively, with IC(50) of camptothecin: 0.3 µM). Compounds 6, 12 and 23 showed cytotoxicities in the HT-29 cell line (IC(50): 19, 19 and 15 µM, respectively, with IC(50) of camptothecin: 2 µM).


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Drug Discovery , Neoplasms/drug therapy , Topoisomerase I Inhibitors/pharmacology , Topoisomerase II Inhibitors/pharmacology , Ulmus/chemistry , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Cell Line, Tumor , Cyclohexanones/chemistry , Cyclohexanones/isolation & purification , Cyclohexanones/pharmacology , Drug Screening Assays, Antitumor , Eicosanoic Acids/chemistry , Eicosanoic Acids/isolation & purification , Eicosanoic Acids/pharmacology , Flavonoids/chemistry , Flavonoids/isolation & purification , Flavonoids/pharmacology , Glucosides/chemistry , Glucosides/isolation & purification , Glucosides/pharmacology , Humans , Inhibitory Concentration 50 , Medicine, Korean Traditional , Molecular Structure , Plant Bark/chemistry , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plant Roots/chemistry , Spectrometry, Mass, Fast Atom Bombardment , Terpenes , Topoisomerase I Inhibitors/chemistry , Topoisomerase I Inhibitors/isolation & purification , Topoisomerase II Inhibitors/chemistry , Topoisomerase II Inhibitors/isolation & purification , Triterpenes/chemistry , Triterpenes/isolation & purification , Triterpenes/pharmacology
4.
J Ethnopharmacol ; 132(1): 143-9, 2010 Oct 28.
Article in English | MEDLINE | ID: mdl-20699114

ABSTRACT

AIM OF THE STUDY: The aerial part of Saururus chinensis has been used in folk medicine to treat several inflammatory diseases in China and Korea. Previously, our group reported that anti-asthmatic activity of an ethanol extract of Saururus chinensis (ESC) might occur, in part, via the inhibition of prostaglandin D(2) (PGD(2)) and leukotriene C(4) (LTC(4)) production, and degranulation reaction in vitro, as well as through the down-regulation of interleukin (IL)-4 and eotaxin mRNA expression in an in vivo ovalbumin-sensitization animal model. However, the effects of Saururus chinensis on eicosanoid generation, as well as Th2 cytokines and eotaxin production in an in vivo asthma model, have not been fully investigated. Moreover, it has not been determined whether ESC can ameliorate airway inflammation in vivo. In the present study, we investigated the therapeutic activity of Saururus chinensis on ovalbumin (OVA)-sensitized airway inflammation and its major phytochemical compositions. MATERIALS AND METHODS: Asthma was induced in BALB/c mice by ovalbumin-sensitization and inhalation. ESC (10-100 mg/kg) or dexamethasone (5 mg/kg), a positive control, was administered 7 times orally every 12 h from one day before the first challenge to 1 h before the second challenge. The recruitment of inflammatory cells and hyperplasia of goblet cells were evaluated by H&E and PAS staining. Levels of Th2 cytokines, eotaxin, PGD(2) and LTC(4) were measured to evaluate the anti-inflammatory activity of ESC in OVA-sensitized mice. Contents of major components were analyzed by HPLC using a reversed-phase C18 column. RESULTS: ESC (10 mg/kg) suppressed allergic airway inflammation by inhibition of the production of IL-4 (P<0.001), IL-5 (P<0.05), IL-13 (P<0.001), eotaxin (P<0.001), PGE(2) (P<0.001), LTC(4) (P<0.001) in lung extract and IgE level (P<0.001) in the serum. In addition, ESC (50 mg/kg) reduced the infiltration of inflammatory cells and hyperplasia of goblet cells in the lung tissues. The anti-inflammatory effect of ESC was comparable to that of the positive control drug, dexamethasone. Its major phytochemical composition includes manassantin A, B and sauchinone. CONCLUSIONS: These results suggest that ESC decreased inflammation and mucus secretion in the OVA-induced bronchial asthma model, and its anti-asthmatic activity may occur in part via the inhibition of Th2 cytokines and eotaxin protein expression, as well as through prostaglandin E(2) (PGE(2)) and leukotriene C(4) (LTC(4)) generation. This effects may be attributed particularly to the presence of manassantin A, B and sauchinone major component evidenced by a HPLC analysis.


Subject(s)
Asthma/drug therapy , Plant Extracts/therapeutic use , Saururaceae/chemistry , Animals , Asthma/immunology , Asthma/pathology , Chromatography, High Pressure Liquid , Cytokines/blood , Cytokines/immunology , Dinoprostone/blood , Dinoprostone/immunology , Disease Models, Animal , Ethanol/chemistry , Female , Hyperplasia , Immunoglobulin E/blood , Immunoglobulin E/immunology , Leukotriene C4/blood , Leukotriene C4/immunology , Lung/drug effects , Lung/immunology , Lung/pathology , Medicine, Korean Traditional , Mice , Mice, Inbred BALB C , Ovalbumin/immunology , Plant Components, Aerial/chemistry , Plant Extracts/isolation & purification , Plant Extracts/pharmacology , Respiratory Mucosa/drug effects , Respiratory Mucosa/immunology , Respiratory Mucosa/pathology
5.
J Ethnopharmacol ; 130(1): 61-9, 2010 Jul 06.
Article in English | MEDLINE | ID: mdl-20420895

ABSTRACT

AIM OF THE STUDY: Traditionally, the stem and root bark of Ulmus davidiana var. japonica (Ulmaceae) are Korean herbal medicines used for anti-inflammatory and anticancer therapy. In this study, we investigated the protective effects of Ulmus davidiana var. japonica ethanolic extract (UD) in a murine asthma model. Furthermore, we determined whether heme oxygenase (HO)-1 is required for the protective activity of UD. MATERIALS AND METHODS: Airways of ovalbumin (OVA)-sensitized mice exposed to OVA challenge developed eosinophilia, mucus hypersecretion and increased cytokine levels. UD was applied 1h prior to OVA challenge. Mice were administered UD orally at doses of 100 and 200mg/kg once daily on days 18-23. Bronchoalveolar lavage fluid (BALF) was collected 48 h after the final OVA challenge. Levels of interleukin (IL)-4 and IL-5 in BALF were measured using enzyme-linked immunosorbent assays (ELISAs). Lung tissue sections 4 microm in thickness were stained with Mayer's hematoxylin and eosin for assessment of cell infiltration and mucus production with PAS (periodic acid shift reagent) staining, in conjunction with ELISA, immunohistochemistry and Western blot analyses for HO-1 protein expression. RESULTS AND CONCLUSION: Orally administered UD significantly inhibited the number of OVA-induced inflammatory cells and IgE production, along with reduced T-helper (Th)2 cytokine levels, such as IL-4 and IL-5, in BALF and lung tissue. In addition, UD induced a marked decrease in OVA-induced reactive oxygen species (ROS), inflammatory cell infiltration and mucus production in lung tissue. These effects were correlated with HO-1 mRNA and protein induction. Our results indicate that UD protects against OVA-induced airway inflammation, at least in part, via HO-1 upregulation.


Subject(s)
Asthma/drug therapy , Heme Oxygenase (Decyclizing)/metabolism , Ovalbumin/pharmacology , Plant Extracts/therapeutic use , Ulmus/chemistry , Up-Regulation/drug effects , Animals , Asthma/chemically induced , Asthma/enzymology , Base Sequence , Blotting, Western , Bronchoalveolar Lavage Fluid , Chromatography, High Pressure Liquid , DNA Primers , Enzyme-Linked Immunosorbent Assay , Female , Immunohistochemistry , Mice , Mice, Inbred BALB C , Plant Extracts/pharmacology , Reactive Oxygen Species/metabolism , Reverse Transcriptase Polymerase Chain Reaction
6.
Arch Pharm Res ; 32(10): 1409-15, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19898804

ABSTRACT

Thirteen lignans, erythro-austrobailignan-6 (1), meso-dihydroguaiaretic acid (2), sauchinone (3), 1'-epi-sauchinone (4), saucerneol D (5), manassantin B (6), manassantin A (7), nectandrin B (8), machilin D (9), saucerneol F (10), saucerneol G (11), saucerneol H (12) and saucerneol I (13), were isolated from the ethyl acetate extract of the roots of Saururus chinensis. Among these compounds, 5 showed potent inhibitory activities against DNA topoisomerase I and II, and 5, 6, 7 and 10 showed mild cytotoxicities against HT-29 (IC(50) values; 13, 12, 11, and 10 microM, respectively) and HepG2 cell lines (IC(50) values; 16, 11, 12, and 11 microM, respectively).


Subject(s)
Enzyme Inhibitors/pharmacology , Lignans/pharmacology , Saururaceae/chemistry , Topoisomerase I Inhibitors , Topoisomerase II Inhibitors , Cell Line, Tumor , Cell Survival/drug effects , Enzyme Inhibitors/isolation & purification , Humans , Lignans/isolation & purification , Molecular Structure , Plant Extracts/isolation & purification , Plant Extracts/pharmacology , Plant Roots/chemistry
7.
Biol Pharm Bull ; 31(3): 523-6, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18310923

ABSTRACT

In the course of isolating preventive agents from sepsis based on the in vivo assay model from the EtOAc extract of the roots of Saururus chinensis, twelve lignans, sarisan (1), erythro-austrobailignan-6 (2), meso-dihydroguaiaretic acid (3), saucerneol B (4), manassantin B (5), manassantin A (6), rel-(8R,8'R)-dimethyl-(7S,7'R)-bis(3,4-methylenedioxyphenyl)tetrahydro-furan (7), (+)-saururinone (8), sauchinone (9), sauchinone B (10), nectandrin B (11) and machilin D (12), were isolated. Compounds 9 and 10, at a dose of 10 mg/kg, increased survival rates to 80% from 20% for the control experiment, and decreased the plasma levels of tumor necrosis factor-alpha (TNF-alpha) and alanine aminotransferase (ALT) activity in mice administered LPS/D-GalN.


Subject(s)
Lignans/therapeutic use , Saururaceae/chemistry , Sepsis/drug therapy , Alanine Transaminase/blood , Animals , Cytokines/blood , Cytokines/immunology , Disease Models, Animal , Lignans/isolation & purification , Lipopolysaccharides/toxicity , Liver/drug effects , Liver/enzymology , Male , Mice , Mice, Inbred ICR , Plant Extracts/chemistry , Plant Roots/chemistry , Sepsis/enzymology , Sepsis/immunology
8.
Planta Med ; 73(12): 1287-91, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17973203

ABSTRACT

Two new benzofurans, 1-furan-2-yl-2-(4-hydroxyphenyl)-ethanone (1) and 5-(4-hydroxybenzyloxymethyl)-furan-2-carbaldehyde (2), together with five known compounds, 4-hydroxybenzyl methyl ether (3), 5-(hydroxymethyl)-furfural (4), gastrodin (5), beta-sitosterol (6) and beta-sitosterol glucoside (7) were isolated from the rhizome of Gastrodia elata Blume. In DNA topoisomerase I and II inhibition assays in vitro at a concentration of 20 microM, 1 showed potent inhibitory activity, 66% and 71% inhibition, respectively, compared to the positive control compounds, camptothecin and etoposide, 71% and 22% inhibition, respectively. All of these compounds exhibited weak or no cytotoxicities against human colon carcinoma cell line (HT-29), human breast carcinoma cell line (MCF-7) and human hepatocellular carcinoma cell line (HepG-2).


Subject(s)
Benzofurans/pharmacology , Gastrodia/chemistry , Topoisomerase I Inhibitors , Topoisomerase II Inhibitors , Antineoplastic Agents/isolation & purification , Antineoplastic Agents/pharmacology , Benzofurans/isolation & purification , Cell Line, Tumor , Humans , Plants, Medicinal/chemistry , Rhizome/chemistry
SELECTION OF CITATIONS
SEARCH DETAIL
...