Analysis of changes in gene expression and metabolic profiles induced by silica-coated magnetic nanoparticles.

Magnetic nanoparticles (MNPs) have proven themselves to be useful in biomedical research; however, previous reports were insufficient to address the potential dangers of nanoparticles. Here, we investigated gene expression and metabolic changes based on the microarray and gas chromatography-mass spectrometry with human embryo kidney 293 cells treated with MNPs@SiO(2)(RITC), a silica-coated MNP containing Rhodamine B isothiocyanate (RITC). In addition, measurement of reactive oxygen species (ROS) and ATP analysis were performed to evaluate the effect of MNPs@SiO(2)(RITC) on mitochondrial function. Compared to the nontreated control, glutamic acid was increased by more than 2.0-fold, and expression of genes related to the glutamic acid metabolic pathway was also disturbed in 1.0 µg/µL of MNPs@SiO(2)(RITC)-treated cells. Furthermore, increases in ROS concentration and mitochondrial damage were observed in this MNPs@SiO(2)(RITC) concentration. The organic acids related to the Krebs cycle were also disturbed, and the capacity of ATP synthesis was decreased in cell treated with an overdose of MNPs@SiO(2)(RITC). Collectively, these results suggest that overdose (1.0 µg/µL) of MNPs caused transcriptomic and metabolic disturbance. In addition, we suggest that a combination of gene expression and metabolic profiles will provide more detailed and sensitive toxicological evaluation for nanoparticles.

Bioinspired polymerization of dopamine to generate melanin-like nanoparticles having an excellent free-radical-scavenging property.

Melanin-like nanoparticles were synthesized with size control through neutralization of dopamine hydrochloride with NaOH, followed by spontaneous air oxidation of dopamine. Although the particle characteristic of natural melanins was understood to be significantly affected by the biological and structural environment, melanin-lke nanoparticles can be realized through the chemical reactions only. Melanin-like nanoparticles that are <100 nm showed excellent dispersion stability in water as well as biological media and good biocompatibility to HeLa cells after the appropriate surface modification with thiol-terminated methoxy-poly(ethylene glycol) (mPEG-SH). Furthermore, the demonstrated ability of melanin-like nanoparticles to reduce 2,2-diphenyl-1-picrylhydrazyl (DPPH) suggests free radical scavenging activity of the material.

Stable biomimetic superhydrophobic surfaces fabricated by polymer replication method from hierarchically structured surfaces of Al templates.

We have developed a simple, efficient, and highly reproducible method to fabricate the large-area biomimetic superhydrophobic polymer surfaces having hierarchical structures of micrometer-sized irregular steps and nanometer-sized fibrils. Commercial Al plates (99.0%) were etched using Beck's dislocation etchant (mixture of HCl and HF) for different time periods in order to alter the structure of the etched Al surfaces from micrometer-sized to highly rough nanometer-sized irregular steps. These hierarchical structures could be easily replicated onto the surface of various thermoplastic polymer plates from the etched Al templates by applying heat and pressure; many polymer replicas without any significant deviations from each other could be duplicated from the same etched Al master templates. All of thermoplastic polymer replicas having hierarchical structures exhibited superhydrophobic properties with water contact angles of larger than 150 degrees. Especially, the surfaces of the high-density polyethylene (HDPE) replicas having nanometer-sized curled strands exhibited superhydrophobicity with a static water contact angle of approximately 160 degrees and a sliding angle of less than 2 degrees. These superhydrophobic HDPE replicas having nanometer-sized curled strands showed excellent stability after being exposed to various organic solvents and aqueous solutions of various pH.

Dual-modal nanoprobes for imaging of mesenchymal stem cell transplant by MRI and fluorescence imaging.

OBJECTIVE: To determine the feasibility of labeling human mesenchymal stem cells (hMSCs) with bifunctional nanoparticles and assessing their potential as imaging probes in the monitoring of hMSC transplantation. MATERIALS AND METHODS: The T1 and T2 relaxivities of the nanoparticles (MNP@SiO(2)[RITC]-PEG) were measured at 1.5T and 3T magnetic resonance scanner. Using hMSCs and the nanoparticles, labeling efficiency, toxicity, and proliferation were assessed. Confocal laser scanning microscopy and transmission electron microscopy were used to specify the intracellular localization of the endocytosed iron nanoparticles. We also observed in vitro and in vivo visualization of the labeled hMSCs with a 3T MR scanner and optical imaging. RESULTS: MNP@SiO(2)(RITC)-PEG showed both superparamagnetic and fluorescent properties. The r(1) and r(2) relaxivity values of the MNP@SiO(2)(RITC)-PEG were 0.33 and 398 mM(-1) s(-1) at 1.5T, respectively, and 0.29 and 453 mM(-1) s(-1) at 3T, respectively. The effective internalization of MNP@SiO(2)(RITC)-PEG into hMSCs was observed by confocal laser scanning fluorescence microscopy. The transmission electron microscopy images showed that MNP@SiO(2)(RITC)-PEG was internalized into the cells and mainly resided in the cytoplasm. The viability and proliferation of MNP@SiO(2)(RITC)-PEG-labeled hMSCs were not significantly different from the control cells. MNP@SiO(2)(RITC)-PEG-labeled hMSCs were observed in vitro and in vivo with optical and MR imaging. CONCLUSION: MNP@SiO(2)(RITC)-PEG can be a useful contrast agent for stem cell imaging, which is suitable for a bimodal detection by MRI and optical imaging.

Acupuncture muscle channel in the subcutaneous layer of rat skin.

Using a mixed-dye injection technique, we found a novel kind of muscle fiber with a lumen, established its precise location in the subcutaneous muscle layer along the acupuncture muscle of the bladder line, and determined its detailed ultrastructure. The channels with flowing liquid were a novel kind of muscle fibers with lumens and they were located in the subcutaneous muscle layer of rat. Their detection was realized by using chrome-hematoxylin and a mixture of fluorescent nanoparticles and commercial Pelikan ink. These acupuncture muscle channels were hidden among the neighboring skin skeletal muscle fibers and were barely distinguishable from them with light microscopes. Only with a transmission electron microscope were their characteristic features shown to be different from normal skin skeletal muscle. These features included undifferentiated muscle fibers that resembled immature myofibrils without Z-lines and reassembled telophase nuclei.