Anatomopathological aspects of avian aspergillosis.

Aspergillosis is a fungal disease caused by fungi of the genus Aspergillus, in particular A. fumigatus and A. flavus. This paper focuses on anatomopathological aspects resulting from a chronic infection from Aspergillus spp in the chicken (Gallus domesticus), in the herring gull (Larus cachinnans micaelli) and in the red-legged partridge (Alectoris rufa rufa). Microscopically, we observed some histological lesions that are related to the two typical forms of Aspergillosis: a deep nodular form, typical of organs with a non-aerated parenchyma, and a non-encapsulated superficial diffuse form typical of the serosae and the lung. The observed forms of aspergillosis have been found in animals raised in poor hygienic environmental conditions or malnourished animals (chicken); in wild birds from wildlife recovery centres (herring gull), which underwent some forms of stress, such as traumas, detention, starvation, extended antibiotic treatments; in game birds (red-legged partridge) used for restocking natural areas that had been negatively affected by such stressors as captivity in aviaries, containment and transport in cages, release in unsuitable environments and malnutrition. The observed anatomopathological and istopathological aspects can therefore be regarded as the outcome of a number of factors that have reduced the typical resistance of the species and impaired the efficiency of their immune systems.

The effects of ovine lentivirus infection on some productive aspects in a Sardinian sheep flock from Italy.

The effects of ovine lentivirus infection on the fat, protein and lactose concentrations on the somatic cell counts in ewes' milk, on milk production and on the birth weight and growth of lambs were studied in a flock of Sardinian sheep from central Italy. Data on 61 lactations of ewes positive to both the agargel immunodiffusion test and an enzyme-linked immunosorbent assay were compared with those on 46 lactations of seronegative ewes. Confounders such as parity, age, lactation length, litter size, and the sex of the lambs were considered. None of these traits seemed to be negatively influenced by the infection.

Isolation and identification of avian rotavirus from pheasant chicks with signs of clinical enteritis.

Three rotaviruses were isolated from intestinal contents obtained from a flock of 6-8-day-old pheasant chicks showing diarrhoea and increased mortality. The isolates were characterized as avian group A rotavirus by immunoenzymatic technique (ELISA) and polyacrylamide gel electrophoresis (PAGE).

Isolation and characterization of cytopathic strains of rotavirus from hares (Lepus europaeus).

Ten cytopathic rotavirus were recovered from stools of leverets affected by an enteric syndrome. The ten isolates, examined by means of ELISA and SDS-PA-GE, were identified as group A rotaviruses.

Molecular epidemiology of bovine rotaviruses. Characterization of rotaviruses isolated from diarrhoeic calves by genome profile analysis.

Fifteen bovine rotavirus group A strains were isolated in several Italian regions over the period 1981-1989 from calves in ten neonatal diarrhoea outbreaks. The electrophoretical analysis of the genoma showed genomic variations and five different profiles were observed, including one with thirteen dsRNA segments. The finding of extra RNA fragments, with respect to the regular eleven genome segments, suggests the possibility of simultaneous or sequential infection by more than one electropherotype or a modification in the length of RNA segments during infection.

Electrophoretic typing of some strains of enteric viruses isolated in rabbits suffering from diarrhoea.

Fifty strains of enteric viruses were isolated on MA-104 cell cultures from the diarrhoeic stools of rabbits from Tuscan and Umbrian breedings. Four of these were identified by means of serological and electrophoretical assays as Group A rotavirus; one produced a negative result in the ELISA test and an electrophoretic pattern related to reovirus.

Isolation and identification of rotaviruses as aetiological agents of neonatal diarrhoea in kids. Electrophoretical characterization by PAGE.

Six strains of cytopathogenic enteric virus for the continuous cell line MA-104 were isolated from samples of the diarrhoeic stools of kids (Capra hyrcus) with acute enteritis. The samples were obtained from two stock farms (RM and SI) in central Italy. Four viruses from the RM stock were identified as rotaviruses of the antigenic group A by the Elisa test performed with SA-11 antirotavirus monoclonal antibodies and the direct immunofluorescence test with IgG bovine antirotavirus. Electrophoretical analysis of the viral dsRNA in these strains showed a pattern of eleven bands arranged in the typical "long pattern" of rotavirus group A. Two electrophoretical variants were identified in two separate cases of neonatal diarrhoea in the RM stock over a period of one year. The other two strains, one isolated in the RM stock and the other in the SI stock, gave negative results in serological tests for antigenic group A rotavirus and showed a double chain RNA consisting of 9-10 segments, unrelated to rotavirus or pararotavirus.

Rotavirus infection in horses. Genome profile analysis of a rotavirus isolated from an infected foal.

Electrophoretic analysis in polyacrylamide gel (PAGE) of the equine rotavirus 106/88/LI/EQ, isolated from the diarrhea of an 18 day old foal was compared to the bovine strain NCDV. There was a notable difference in the migration of some segments of the viral RNA. Bands 2 and 3 of the equine rotavirus comigrated while there was a clear separation of segments 7, 8 and 9. Moreover, the migration of segments 1, 4 and 5 revealed a lower molecular weight than the corresponding segments of NCDV.

Caprine arthritis-encephalitis. Virus isolation and identification in goat herds in Italy.

Caprine arthritis-encephalitis virus (CAEV) was isolated by explant cultures of carpal synovial membrane, udder, lymph-nodes, lung and choroid plexus from six goats in two different herds with histories of arthritis-encephalitis. The virus was also isolated from milk, heparinized blood and carpal synovial fluid, using secondary goat synovial membrane cells. Virus identification was made by means of cytopathology, agar gel immunodiffusion test, immunofluorescence, in vitro host range and electron microscopy. Efficacy of different virus isolation procedures from live and necropsied animals are discussed.