Comprehensive assessment of copper's effect on marine organisms under ocean acidification and warming in the 21st century.

Copper (Cu) has sparked widespread global concern as one of the most hazardous metals to aquatic animals. Ocean acidification (OA) and warming (OW) are expected to alter copper's bioavailability based on pH and temperature-sensitive effects; research on their effects on copper on marine organisms is still in its infancy. Therefore, under representative concentration pathways (RCP) 2.6, 4.5, and 8.5, we used the multiple linear regression-water quality criteria (MLR-WQC) method to assess the effects of OA and OW on the ecological risk posed by copper in the Ocean of East China (OEC), which includes the Bohai Sea, Yellow Sea, and East China Sea. The results showed that there was a positive correlation between temperature and copper toxicity, while there was a negative correlation between pH and copper toxicity. The short-term water quality criteria (WQC) values were 1.53, 1.41, 1.30 and 1.13 µg·L-1, while the long-term WQC values were 0.58, 0.48, 0.40 and 0.29 µg·L-1 for 2020, 2099-RCP2.6, 2099-RCP4.5 and 2099-RCP8.5, respectively. Cu in the OEC poses a moderate ecological risk. Under the current copper exposure situation, strict intervention (RCP2.6) only increases the ecological risk of copper exposure by 20 %, and no intervention (RCP8.5) will increase the ecological risk of copper exposure by nearly double. The results indicate that intervention on carbon emissions can slow down the rate at which OA and OW worsen the damage copper poses to marine creatures. This study can provide valuable information for a comprehensive understanding of the combined impacts of climate change and copper on marine organisms.

Comprehensive effects of salinity, dissolved organic carbon and copper on mortality, osmotic regulation and bioaccumulation of copper in Oryzias melastigma.

Cu, as an essential and toxic element, has gained widespread attention. Both salinity and dissolved organic carbon (DOC) are known to influence Cu toxicity in marine organisms. However, the intricate interplay between these factors and their specific influence on Cu toxicity remains ambiguous. So, this study conducted toxicity tests of Cu on Oryzias melastigma. The experiments involved three salinity levels (10, 20, and 30 ppt) and three DOC levels (0, 1, and 5 mg/L) to comprehensively investigate the underlying mechanisms of toxicity. The complex toxic effects were analyzed by mortality, NKA activity, net Na+ flux and Cu bioaccumulation in O. melastigma. The results indicate that Cu toxicity is notably influenced by both DOC and salinity. Interestingly, the discernible variation in Cu toxicity across different DOC levels diminishes as salinity levels increase. The presence of DOC enhances the impact of salinity on Cu toxicity, especially at higher Cu concentrations. Additionally, Visual MINTEQ was utilized to elucidate the chemical composition of Cu, revealing that DOC had a significant impact on Cu forms. Furthermore, we observed that fluctuations in salinity lead to the inhibition of Na+/K+-ATPase (NKA) activity, subsequently hindering the inflow of Na+. The effects of salinity and DOC on the bioaccumulation of copper were not significant. The influence of salinity on Cu toxicity is mainly through its effect on the osmotic regulation and biophysiology of O. melastigma. Additionally, DOC plays a crucial role in the different forms of Cu. Moreover, DOC-Cu complexes can be utilized by organisms. This study contributes to understanding the mechanism of copper's biological toxicity in intricate marine environments and serves as a valuable reference for developing marine water quality criteria for Cu.

Black phosphorus-incorporated novel Ti-12Mo-10Zr implant for multimodal treatment of osteosarcoma.

The repair and reconstruction of large bone defects after bone tumor resection is still a great clinical challenge. At present, orthopedic implant reconstruction is the mainstream treatment for repairing bone defects. However, according to clinical feedback, local tumor recurrence and nonunion of bone graft are common reasons leading to the failure of bone defect repair and reconstruction after bone tumor resection, which seriously threaten the physical and mental health of patients. On this basis, here the self-developed low modulus Ti-12Mo-10Zr alloy (TMZ) was chosen as substrate material. To improve its biological activity and osteointegration, calcium, oxygen, and phosphorus co-doped microporous coating was prepared on TMZ alloy by microarc oxidation (MAO). Then, black phosphorus (BP) nanosheets were incorporated onto MAO treated TMZ alloy to obtain multifunctional composites. The obtained BP-MAO-TMZ implant exhibited excellent photothermal effects and effective ablation of osteosarcoma cancer cells under the irradiation of 808 nm near infrared laser, while no photothermal or therapeutic effects were observed for TMZ alloy. Meanwhile, the structure/component bionic coating obtained after MAO treatment as well as the P-driven in situ biomineralization performance after incorporation of BP nanosheets endowed BP-MAO-TMZ implant with synergistic promoting effect on MC3T3-E1 osteoblasts' activity, proliferation and differentiation ability. This study is expected to provide effective clinical solutions for problems of difficult bone regeneration and tumor recurrence after tumor resection in patients with bone tumors and to solve a series of medical problems such as poor prognosis and poor postoperative quality of patients life with malignant bone tumors.

Astaxanthin alleviates chronic prostatitis/chronic pelvic pain syndrome by increasing colonization of Akkermansia muciniphila in the intestine.

BACKGROUND: Astaxanthin (AST) is a natural compound with anti-inflammatory/immunomodulatory properties that has been found to have probiotic properties. However, the role and mechanism of AST in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) are still not fully understood. PURPOSE: The aim of this study was to evaluate the effect of AST on CP/CPPS and elucidate the mediating role of the gut microbiota. MATERIALS AND METHODS: An experimental autoimmune prostatitis (EAP) mouse model was utilized to test the potential role of AST on CP/CPPS. Antibiotic cocktail (ABX) treatment and fecal microbiota transplantation (FMT) were used to elucidate the gut microbiota-mediated effects on AST. In addition, 16S rRNA gene sequencing and qRT-PCR analyses were used to analyze changes in the gut microbiota of EAP mice and CP/CPPS patients. Finally, the mechanism by which AST exerts a protective effect on CP/CPPS was explored by untargeted metabolomics and gut barrier function assays. RESULTS: Oral administration of AST reduced prostate inflammation scores, alleviated tactile sensitization of the pelvic region in EAP mice, reduced CD4+ T cell and CD68+ macrophage infiltration in the prostatic interstitium, and inhibited the up-regulation of systemic and localized pain/pro-inflammatory mediators in the prostate. After ABX, the protective effect of AST against CP/CPPS was attenuated, whereas colonization with fecal bacteria from AST-treated EAP mice alleviated CP/CPPS. 16S rRNA gene sequencing and qRT-PCR analyses showed that Akkermansia muciniphila in the feces of EAP mice and CP/CPPS patients showed a trend toward a decrease, which was associated with poor progression of CP/CPPS. In contrast, oral administration of AST increased the relative abundance of A. muciniphila, and oral supplementation with A. muciniphila also alleviated inflammation and pain in EAP mice. Finally, we demonstrated that both AST and A. muciniphila interventions increased serum levels of SCFAs acetate, up-regulated expression of colonic tight junction markers, and decreased serum lipopolysaccharide levels in EAP mice. CONCLUSION: Our results showed that AST improved CP/CPPS by up-regulating A. muciniphila, which provides new potentially effective strategies and ideas for CP/CPPS management.

A comprehensive review of the effects of salinity, dissolved organic carbon, pH, and temperature on copper biotoxicity: Implications for setting the copper marine water quality criteria.

In recent years, there has been a growing concern about the ecological hazards associated with copper, which has sparked increased interest in copper water quality criteria (WQC). The crucial factors affecting the bioavailability of copper in seawater are now acknowledged to be salinity, dissolved organic carbon (DOC), pH, and temperature. Research on the influence of these four water quality parameters on copper toxicity is rapidly expanding. However, a comprehensive and clear understanding of the relevant mechanisms is currently lacking, hindering the development of a consistent international method to establish the seawater WQC value for copper. As a response to this knowledge gap, this study presents a comprehensive summary with two key focuses: (1) It meticulously analyzes the effects of salinity, DOC, pH, and temperature on copper toxicity to marine organisms. It takes into account the adaptability of different species to salinity, pH and temperature. (2) Additionally, the study delves into the impact of these four water parameters on the acute toxicity values of copper on marine organisms while also reviewing the methods used in establishing the marine WQC value of copper. The study proposed a two-step process: initially zoning based on the difference of salinity and DOC, followed by the establishment of Cu WQC values for different zones during various seasons, considering the impacts of water quality parameters on copper toxicity. By providing fundamental scientific insights, this research not only enhances our understanding and predictive capabilities concerning water quality parameter-dependent Cu toxicity in marine organisms but also contributes to the development of copper seawater WQC values. Ultimately, this valuable information facilitates more informed decision-making in marine water quality management efforts.

[Spatiotemporal Variation Characteristics of Main Pollutant Fluxes in the Yangtze River Basin from 2017 to 2020].

Based on the monitoring data of various pollutants at important water system points in the Yangtze River Basin from 2017 to 2020, research on the flux change law of the main and tributary water systems in the Yangtze River Basin was carried out at the spatiotemporal scale, and the spatial change response, interannual change trend, and flux correlation analysis were analyzed from the aspects of water quantity, quality, and flux so as to reveal the spatial-temporal contribution characteristics of pollutant flux in the upper, middle, and lower reaches of the Yangtze River Basin. The results showed that over the past four years, the concentration of major pollutants in the Yangtze River Basin showed an overall downward trend. The concentration of total phosphorus (TP) and ammonia (NH+4-N) decreased significantly. The concentration of total nitrogen (TN) and total phosphorus (TP) in the main stream gradually increased from west to east. The annual concentration of permanganate index in the upper, middle, and lower reaches decreased by 18.5%, 16.0%, and 14.0%, respectively, from 2017 to 2020, with the highest decline in the upper reaches. The four-year average value of the spatial distribution of runoff significantly increased from 466×108 m3 to 9923×108 m3. The two lake basins in the tributary river lake water system had the largest contribution to the water volume. The fluxes of permanganate index, total phosphorus (TP), and total nitrogen (TN) among the main pollutants showed an annual increase and then decrease trend. The pollutant fluxes in the Minjiang River, Tuojiang River, Jialing River, and the middle reaches of the two lakes contributed greatly to the river inflow. There were differences in fluxes in different regional water environments. The results of correlation and hierarchical cluster analysis showed that the permanganate index and TP fluxes were highly significantly correlated with water volume, and there was a significant correlation between biochemical oxygen demand (BOD5) and total nitrogen (TN), total phosphorus (TP), and chemical oxygen demand (COD). The main pollutants showed strong differences in the flood and non-flood periods and had a significant response in the flood season from July to September. The research results can provide scientific basis and theoretical support for the integrated management and precise prevention and control of the aquatic environment in the Yangtze River Basin.

Platinum based theranostics nanoplatforms for antitumor applications.

Platinum (Pt) based nanoplatforms are biocompatible nanoagents with photothermal antitumor performance, while exhibiting excellent radiotherapy sensitization properties. Pt-nanoplatforms have extensive research prospects in the realm of cancer treatment due to their highly selective and minimally invasive treatment mode with low damage, and integrated diagnosis and treatment with image monitoring and collaborative drug delivery. Platinum based anticancer chemotherapeutic drugs can kill tumor cells by damaging DNA through chemotherapy. Meanwhile, Pt-nanoplatforms also have good electrocatalytic activity, which can mediate novel electrodynamic therapy. Simultaneously, Pt(II) based compounds also have potential as photosensitizers in photodynamic therapy for malignant tumors. Pt-nanoplatforms can also modulate the immunosuppressive environment and synergistically ablate tumor cells in combination with immune checkpoint inhibitors. This article reviews the research progress of platinum based nanoplatforms in new technologies for cancer therapy, starting from widely representative examples of platinum based nanoplatforms in chemotherapy, electrodynamic therapy, photodynamic therapy, photothermal therapy, and immunotherapy. Finally, multimodal imaging techniques of platinum based nanoplatforms for biomedical diagnosis are briefly discussed.

Multiple isotopes reveal the driving forces of nitrogen cycling from freshwater to brackish water.

Rivers play a crucial role in global nitrogen (N) cycling, but revealing the driving mechanism of N cycling remains challenging because of the complex natural background gradients. The Qiantang River Basin provides an opportunity to elucidate the driving mechanism due to the complex climatic and hydrological conditions. In this study, the multiple stable isotopes suggested that the conservative mixing of two end members was insufficient to explain the complex behavior of N in both seasons. In-soil processes were the primary N cycling processes that controlled riverine N loading during the wet season, whereas in-stream N biological transformation processes (nitrification and assimilation) were more prevalent during the dry season. The results of MixSIAR revealed that soil sources (soil N and N fertilizer) contributed the most to NO3- during the wet season, accounting for 64.3 %, followed by manure and sewage (31.6 %) and atmospheric precipitation (4.1 %). During the dry season, manure and sewage were the predominant contributors to NO3- (52.1 %), followed by soil N (26.6 %), N fertilizer (18.8 %), and atmospheric precipitation (2.5 %). The relationships between d-excess and δ15N-NH4+ or δ15N-NO3- suggested that both climatic and hydrological conditions would be the driving forces regulating the N transportation and transformation in this basin, leading to the high spatiotemporal heterogeneity in N loading and isotopic compositions. In the wet season, precipitation patterns served as the primary driving forces regulating in-soil biological processes and soil leaching. While the hydrological conditions, especially water residence time, were the crucial factors controlling in-stream biological processes during the dry season. This study elucidates N sources, biotransformation processes, and their driving forces from freshwater to brackish water, which has applications for understanding the N fate from terrene to ocean.

Comparison of the retinal microvasculature between compressive and glaucomatous optic neuropathy.

PURPOSE: To compare the patterns of retinal microvasculature change in the peripapillary and macular region between compressive optic neuropathy (CON) and glaucomatous optic neuropathy (GON), and to assess the ability of optical coherence tomography angiography (OCTA) in differentiating the two conditions. METHODS: This cross-sectional study included 108 participants (108 eyes), 36 with CON, 36 with GON, and 36 healthy controls. The CON and GON eyes were matched by the average peripapillary retinal nerve fiber layer (pRNFL) thickness (1:1). Optical coherence tomography (OCT) and OCTA were performed to compare the structural and vascular change of the peripapillary and macular region between groups. RESULTS: Both CON and GON eyes showed more severe structural and vascular damage than the control eyes. The CON eyes had lower pRNFL thickness than the GON eyes in the temporal and nasal quadrants, and thicker pRNFL thickness in the inferior quadrant. The average GCC thickness did not differ between the two groups. The peripapillary vessel density of the CON group was significantly higher in the inferior sectors than that of the GON group. In the macular region, the CON group had significantly higher vessel density in the whole image, the temporal sector in parafovea region, and the temporal, superior, and inferior sectors in perifovea region. CONCLUSION: To a similar degree of structural damage, CON had less retinal vascular impairment than GON, especially in the macular region, and the significance of the finding needs further evaluation.

Exploration of the factors that influence total phosphorus in surface water and an evaluation of surface water vulnerability based on an advanced algorithm and traditional index method.

Due to the continuous influence of human activities, phosphorus pollution in surface water has become a persistent problem that needs to be addressed since phosphorous entails certain risks and degrees of damage to ecosystems and humans. The presence and accumulation of total phosphorus (TP) concentrations in surface waters is the result of a combined effect of many natural and anthropogenic factors, and it is often difficult to intuitively identify the individual importance of each factor in regard to the pollution of the aquatic environment. Considering these issues, this study provides a new methodology to better understand the vulnerability of surface water to TP pollution and the factors that influence TP pollution through the application of two modeling approaches. This includes the boosted regression tree (BRT), an advanced machine learning method, and the traditional comprehensive index method (CIM). Different factors, such as natural variables (including slope, soil texture, normalized difference vegetation index (NDVI), precipitation, and drainage density) and point and nonpoint source anthropogenic factors were included to model the vulnerability of surface water to TP pollution. Two methods were used to produce a vulnerability map of surface water to TP pollution. Pearson correlation analysis was used to validate the two vulnerability assessment methods. The results showed that BRT was more strongly correlated than CIM. In addition, the importance ranking results showed that slope, precipitation, NDVI, decentralized livestock farming and soil texture had a greater influence on TP pollution. Industrial activities, scale livestock farming and population density, which are all contributing sources of pollution, were all relatively less important. The introduced methodology can be used to quickly identify the area most vulnerable to TP pollution and to develop problem specific adaptive policies and measures to reduce the damage from TP pollution.

Environmentally adaptive polysaccharide-based hydrogels and their applications in extreme conditions: A review.

Polysaccharide hydrogels are one of the most promising hydrogel materials due to their inherent characteristics, including biocompatibility, biodegradability, renewability, and easy modification, and their structure and functional designs have been widely researched to adapt to different application scenarios as well as to broaden their application fields. As typical wet-soft materials, the high water content and water-absorbing ability of polysaccharide-based hydrogels (PHs) are conducive to their wide biomedical applications, such as wound healing, tissue repair, and drug delivery. In addition, along with technological progress, PHs have shown potential application prospects in some high-tech fields, including human-computer interaction, intelligent driving, smart dressing, flexible sensors, etc. However, in practical applications, due to the poor ability of PHs to resist freezing below zero, dehydration at high temperature, and acid-base/swelling-induced deformation in a solution environment, they are prone to lose their wet-soft peculiarities, including structural integrity, injectability, flexibility, transparency, conductivity and other inherent characteristics, which greatly limit their high-tech applications. Hence, reducing their freezing point, enhancing their high-temperature dehydration resistance, and improving their extreme solution tolerance are powerful approaches to endow PHs with multienvironmental adaptability, broadening their application areas. This report systematically reviews the study advances of environmentally adaptive polysaccharide-based hydrogels (EAPHs), comprising anti-icing hydrogels, high temperature/dehydration resistant hydrogels, and acid/base/swelling deformation resistant hydrogels in recent years. First, the construction methods of EAPHs are presented, and the mechanisms and properties of freeze-resistant, high temperature/dehydration-resistant, and acid/base/swelling deformation-resistant adaptations are simply demonstrated. Meanwhile, the features of different strategies to prepare EAPHs as well as the strategies of simultaneously attaining multienvironmental adaptability are reviewed. Then, the applications of extreme EAPHs are summarized, and some meaningful works are well introduced. Finally, the issues and future outlooks of PH environment adaptation research are elucidated.

Discriminating Between Compressive Optic Neuropathy With Glaucoma-Like Cupping and Glaucomatous Optic Neuropathy Using OCT and OCTA.

Purpose: To discriminate between compressive optic neuropathy with glaucoma-like cupping (GL-CON) and glaucomatous optic neuropathy (GON) by comparing the peripapillary retinal nerve fiber layer (pRNFL) thickness and retinal microvasculature using optical coherence tomography (OCT) and optical coherence tomography angiography (OCTA). Methods: In this retrospective cross-sectional study, OCT scans were performed on 28 eyes of GL-CON, 34 eyes of GON, and 41control eyes to determine the pRNFL thickness, ganglion cell complex thickness, and cup/disc ratio. OCTA scans were conducted for 12 eyes of GL-CON, 15 eyes of GON, and 15 control eyes to measure the vessel density of the peripapillary and macular areas. Analysis of covariance was used to perform the comparisons, and the area under the curve was calculated. Results: The GON eyes had a significantly thinner pRNFL in the inferior quadrant and greater vertical cup/disc ratio than the GL-CON eyes. In the radial peripapillary capillary segment, the vessel density of the GON in the inferior sectors was significantly lower than in the GL-CON. The superficial macular vessel density in the whole-image, peritemporal, perinasal, and peri-inferior sectors was significantly smaller in the GON group than in the GL-CON group. The best parameter for discriminating between GL-CON and GON was the superficial macular vessel density in the peritemporal sector. Conclusions: GL-CON eyes showed a characteristic pattern of pRNFL and retinal microvascular changes. Translational Relevance: GL-CON can be effectively distinguished from GON by detecting the alterations in the pRNFL and retinal microvasculature using OCT and OCTA.

Use of non-linear multiple regression models for setting water quality criteria for copper: Consider the effects of salinity and dissolved organic carbon.

Cu pollution is a global concern because of its high toxicity and persistence. Few investigations have been conducted on the effects of salinity and dissolved organic carbon (DOC) on Cu toxicity and water quality criteria (WQC). To analyze their impact on the WQC of Cu, non-linear multiple regression (NLMR) models based on salinity and DOC were constructed. The NLMR models demonstrated that when salinity increased, the toxicity values for Cu on fish, mollusca, rotifer, and echinodermata first increased and then declined, whereas those for arthropoda and algae increased. These findings demonstrate that salinity has a substantial impact on Cu toxicity, primarily owing to changes in physiological activity. The original and corrected WQC values in the upper, middle, and outer regions of the Yangtze Estuary were derived based on the species sensitivity distribution method. These values were 1.49, 3.49, 8.86, and 0.87 µg·L-1. An important finding was that lower Cu concentrations in the outer areas posed the highest ecological risk owing to the effects of salinity and DOC. NLMR models are applicable to other coastal areas worldwide. This provides valuable information for the establishment of an accurate and protective estuary for Cu-related WQC.

OONO-/MMP2/MMP9 pathway-mediated apoptosis of porcine granulosa cells is associated with DNA damage.

In brief: The apoptosis of granulosa cells (GCs) is the main reason for porcine follicular atresia. This study provides a novel mechanism for peroxynitrite anion-mediated GC apoptosis and follicular atresia in porcine ovary. Abstract: Granulosa cells play a crucial role in the development of follicles, and their cell apoptosis in the porcine ovary is a major contributor to follicular atresia. Here, we provide a new mechanism for follicular atresia by describing a crucial mechanism by which peroxynitrite anion (OONO-) may cause GC death. We discovered that nitric oxide, oxidative stress level, and OONO- were positively correlated with porcine follicular atresia, which was accompanied by high expression of matrix metalloproteinase 2 (MMP2) and MMP9. We created a model of OONO--induced apoptosis in GCs and discovered that OONO- could boost the expression of MMP2 and MMP9 and increase the expression of pro-apoptotic proteins and DNA damage. Furthermore, by inhibiting the activities of MMP2 and MMP9, we found that SB-3CT (a specific inhibitor for MMP2 and MMP9) alleviated the decrease in cell survival rates and DNA damage caused by OONO-, which may have been impacted by reducing the cleavage of PARP1 by MMP2 and MMP9. Therefore, our findings imply that OONO- can cause DNA damage to GCs, participating in mediating the expression of pro-apoptotic proteins and inhibiting DNA repair by preventing the activity of PARP1 through MMP2 and MMP9. These results help explain how OONO-/MMP2/MMP9 affects porcine follicular atresia and GC apoptosis.

Improvement in water pollution control alters nutrient stoichiometry of Guanting Reservoir near Beijing, North China.

Significant improvement in wastewater treatment is the most effective way for eutrophication control, especially in semiarid regions. However, its effect on the nutrient status and stoichiometry of the receiving water body has remained poorly considered and understood at broad temporal scales. Taking Guanting Reservoir (GR) in Hebei-Beijing (P. R. China) as an example, we present a study that links a continuous monitoring dataset for GR with corresponding estimates of human-induced nutrient discharges in its watershed from the year 2006 to 2019. We find that current GR belongs to strict P limitation and the faster decrease of TP than TN concentrations and continuous increase of TN/TP mass ratios in GR are attributed to the water restoration investment-induced declining of nutrient loadings. The improved municipal wastewater treatment capacity is mainly responsible for these significant changes, due to the higher removal efficiency of TP than TN in municipal wastewater. Given the potential ecological impact on aquatic biodiversity as well as ecosystem function of changes in TN/TP ratios and higher retention rate of TP (97.4%) compared with TN (93.1%) in GR, our findings highlight that future strategy for water pollution control should not only concentrate on more nutrient reduction efficiencies but attach importance to their stoichiometric balance to reduce the potential risk of phytoplankton blooms and toxin production during the water quality recovery of lakes or reservoirs.

Dietary curcumin supplementation relieves hydrogen peroxide-induced testicular injury by antioxidant and anti-apoptotic effects in roosters.

This study was aimed to investigate the effects of dietary curcumin supplementation on the hydrogen peroxide (H2O2)-induced testicular oxidative damage in breeder roosters. Thirty-two 20-week roosters were randomly divided into four groups: (1) basal diet (CON); (2) basal diet with H2O2 challenge (H2O2); (3) basal diet with 200 mg/kg curcumin (CUR); (4) basal diet with 200 mg/kg curcumin and H2O2 challenge (CUR + H2O2). The trial lasted for 8 weeks, H2O2 challenged groups got an intraperitoneal injection of H2O2 at the 50 and 53 days, while the CON and CUR groups received an injection of saline. The results showed that dietary curcumin supplementation significantly decreased abnormal sperm rates in the semen, notably improved seminiferous tubules, increased testis scores, and serum testosterone levels. Curcumin supplementation could also ameliorate the redox damage caused by H2O2, by enhancing the capacities of antioxidant enzymes (CAT, GSH-Px, SOD, and T-AOC), and reducing MDA levels. In addition, curcumin normalized the H2O2-induced negative effects, which included downregulations in spermatogenesis-related genes (STAR, HSD3-ß1, SYCP3, AKT1) and antioxidant genes (HMOX-1, NQO-1), reduced protein expressions of Nrf2, PCNA, and Bcl-2, and increased protein expressions of Caspase 3 and Bax. Moreover, H2O2-induced decreased mRNA expressions of EIF2AK3, Caspase3, and BCL-2 were all reversed by dietary curcumin supplementation. In summary, dietary curcumin supplementation could relieve H2O2-induced oxidative damage and reproduction decline through the Nrf2 signaling pathway and anti-apoptotic effects in roosters.

Salinity-dependent aquatic life criteria of inorganic mercury in coastal water and its ecological risk assessment.

Mercury (Hg) is one of the most toxic pollutants to aquatic organisms. The influence of salinity on Hg toxicity, an important factor restricting the development of global marine aquatic life criteria (ALC), is unclear. Therefore, mercury toxicity data were corrected based on salinity using the aggregate slope method, and the ALC values were derived. Short-term aquatic life criteria (SALC) and long-term aquatic life criteria (LALC) were derived using the species sensitivity distribution method based on Log-logistic, Log-normal, Burr III, Gumbel, and Weibull models. The hazard quotient (HQ) and joint probability curve (JPC) methods were used to evaluate the ecological risk of Hg in the coastal waters of China. The results showed that the SALC and LALC of Hg in the coastal waters of China were 2.21 and 0.54 µg/L. The toxicity data and salinity were positively correlated for Chordate and Arthropoda and negatively correlated for Mollusca. The SALC values increased by approximately 75%, with salinities ranging from 10 to 20 ppt. A slight peak in the SALC at mid-salinities was also observed. The ecological risk assessment of Hg in China's coastal waters showed that attention should be paid to Hg pollution in the Bohai Sea and East China Sea, especially the ecological risk of Hg to crustacean organisms. This study could promote the development of water quality criteria for coastal waters and provide a technical reference for mercury management in the coastal waters of China.

Highly stretchable, self-healing elastomer hydrogel with universal adhesion driven by reversible cross-links and protein enhancement.

Engineered hydrogels with excellent mechanical properties and multi-functionality have great potential as soft electronic skins, tissue substitutes and flexible robotic joints. However, it has been a challenge to construct multifunctional hydrogels, especially when integrating high stretchability, toughness and strength, low hysteresis, good self-healing and adhesion abilities into a hydrogel system simultaneously. Here, we successfully developed a structural hydrogel composed of a reversible covalently cross-link-based poly-N-(2-hydroxyethyl)acrylamide (PHEMAA) network and available plastically deformable casein micelles. Such a design enabled the reversible covalent cross-links and casein micelles to enhance energy dissipation and toughen the PHEMAA/casein hybrid hydrogel synergistically. More importantly, the hydrogel could respond to the imposed strains reversibly by cross-link and micelle deformation induced-network reconstitution, which led to low hysteresis of the hydrogels. The recoverable gel networks still exhibited their effects on energy dissipation at the stress-focused area, endowing the hydrogels with fatigue resistance. As a result, the hydrogels exhibited a compressive strength of 36.5 MPa, high stretchability (1460%), high toughness (∼5.98 MJ m-3), low hysteresis (<30%) and fatigue resistance with almost completely overlapped hysteresis curves during 10 loading cycles. In addition, the introduction of casein micelles and reversible covalent bonding endowed the elastomer hydrogels with high adhesivity, self-healing abilities and biocompatibility.

Applications of phase change materials in smart drug delivery for cancer treatment.

Phase change materials (PCMs) are materials that are stimulated by the external enthalpy change (temperature) to realize solid-liquid and liquid-solid phase transformation. Due to temperature sensitivity, friendly modification, and low toxicity, PCMs have been widely used in smart drug delivery. More often than not, the drug was encapsulated in a solid PCMs matrix, a thermally responsive material. After the trigger implementation, PCMs change into a solid-liquid phase, and the loading drug is released accordingly. Therefore, PCMs can achieve precise release control with different temperature adjustments, which is especially important for small molecular drugs with severe side effects. The combination of drug therapy and hyperthermia through PCMs can achieve more accurate and effective treatment of tumor target areas. This study briefly summarizes the latest developments on PCMs as smart gate-keepers for anti-tumor applications in light of PCMs becoming a research hot spot in the nanomedicine sector in recent years.